LOCUS pDGB3_Ω1-NPTII_T1T5_StGFP_StPPE2 22358 bp DNA circular SYN 06-NOV-2024 DEFINITION o1_NPTII_T1T5_StGFP_StPPE2. ACCESSION VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid. REFERENCE 1 (bases 1 to 22358) AUTHORS SnapGene License TITLE Direct Submission JOURNAL Exported Oct 21, 2024 from SnapGene 7.2.1 https://www.snapgene.com FEATURES Location/Qualifiers misc_feature 1..11 /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" terminator complement(33..300) /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (NOST)" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="2 Hygromycin" /Transferred_Similarity="99.63%" /Primary_Match="99.63%; Adjusted-Interval-0:0/0; tNOS (2 Hygromycin)" /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; tNOS (3 Kanamycin)" /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; tNOS (1 Basta)" /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; tNOS (tNOS v2)" /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /Alternative_Match="99.60%; Adjusted-Interval-0:-13/2; tNOS (tNOS v1)" /label="tNOS" Plant_Selection complement(33..1406) /Color="#006600" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Kanamycin" /Transferred_Similarity="99.64%" /Primary_Match="99.64%; Adjusted-Interval-0:0/0; Kana (Kanamycin)" /Alternative_Match="98.63%; Adjusted-Interval-0:15/0; Kana (3 Kanamycin)" /label="Kana" CDS complement(330..1121) /codon_start=1 /note="/vntifkey=4" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" /label="NptII" promoter complement(1134..1412) /created_by="thjac" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pNOS" /X-Transferred_Similarity="96.53%" /X-Primary_Match="96.53%; Adjusted-Interval-0:0/0; pNOS" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pNOS v1" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (pNOS v1)" /Alternative_Match="99.63%; Adjusted-Interval-0:-6/0; pNOS (Basta)" /Alternative_Match="99.63%; Adjusted-Interval-0:-6/0; pNOS (pNOS v2)" /Alternative_Match="99.63%; Adjusted-Interval-0:-6/0; pNOS (Hygromycin)" /Alternative_Match="99.63%; Adjusted-Interval-0:-6/0; pNOS (Kanamycin)" /Alternative_Match="97.88%; Adjusted-Interval-0:4/0; pNOS (2 Hygromycin)" /label="pNOS" promoter 1425..1743 /label="StU6 promoter" misc_feature 1747..1767 /label="21bp Target" Scaffold 1768..1853 /label="esgRNA" misc_feature 1854..1872 /label="RT template" Change 1855 /label="Silent snp" Change 1865..1867 /label="S72K" Change 1868..1870 /label="P73S" misc_feature 1873..1883 /label="PBS" Linker 1884..1891 /label="Peg-Linker" misc_feature 1892..1977 /note="TYMV TLS" terminator 1978..1997 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 2010..2328 /label="StU6 promoter" misc_feature 2332..2352 /label="21bp Target" Scaffold 2353..2438 /label="esgRNA" misc_feature 2439..2453 /label="RT template" Change 2443..2444 /label="Silent snp" Change 2451..2453 /label="A76D" misc_feature 2454..2464 /label="PBS" Linker 2465..2472 /label="Peg-Linker" misc_feature 2473..2558 /note="TYMV TLS" terminator 2559..2578 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 2591..2909 /label="StU6 promoter" misc_feature 2914..2933 /label="20bp Target" Scaffold 2934..3019 /label="esgRNA" misc_feature 3020..3047 /label="RT template" Change 3031 /label="Silent SNP" Change 3037 /label="Silent SNP" Change 3040..3042 /label="G110R" Change 3046 /label="Silent SNP" misc_feature 3048..3057 /label="PBS" Linker 3058..3065 /label="Peg-Linker" misc_feature 3066..3151 /note="TYMV TLS" terminator 3152..3171 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 3184..3502 /label="StU6 promoter" misc_feature 3506..3525 /label="20bp Target" Scaffold 3526..3611 /label="esgRNA" misc_feature 3612..3639 /label="Rt template" Change 3621..3622 /label="S172K" Change 3630 /label="Silent SNP" Change 3633 /label="Silent SNP" Change 3636 /label="Silent SNP" misc_feature 3640..3650 /label="PBS" Linker 3651..3658 /label="Peg-Linker" misc_feature 3659..3744 /note="TYMV TLS" terminator 3745..3764 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 3777..4095 /label="StU6 promoter" misc_feature 4100..4119 /label="20bp Target" Scaffold 4120..4205 /label="esgRNA" misc_feature 4206..4233 /label="RT template" Change 4217 /label="Silent SNP" Change 4221 /label="D112G" Change 4223 /label="Silent SNP" Change 4226 /label="Silent SNP" Change 4232 /label="Silent SNP" misc_feature 4234..4243 /label="PBS" Linker 4244..4251 /label="Peg-Linker" misc_feature 4252..4337 /note="TYMV TLS" terminator 4338..4357 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 4370..5379 /label="PromStUbiQ10" 5'UTR 5380..5478 intron 5479..5972 NLS 5980..6033 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS BP" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP (NLS BP)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; BP (NLS BP translation)" /label="BP" CDS 6034..10134 /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AtCas9-H840A" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AtCas9-H840A" /Alternative_Match="99.98%; Adjusted-Interval-0:0/0; AtCas9-D10A,H840A" /Alternative_Match="99.95%; Adjusted-Interval-0:0/0; AtCas9" /Alternative_Match="99.93%; Adjusted-Interval-0:0/0; AtCas9-D10A" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SpCas9(H840A)" /Alternative_Match="99.93%; Adjusted-Interval-0:0/0; SpCas9" /Alternative_Match="99.93%; Adjusted-Interval-0:0/0; SpCas9(D10A,H840A)" /Alternative_Match="99.85%; Adjusted-Interval-0:0/0; SpCas9(D10A)" /label="AtCas9-H840A" NLS 10156..10176 /created_by="warddecaestecker" /modified_by="wacae" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS SV40" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /label="SV40" Linker 10183..10278 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="32-aa linker" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 32-aa linker" /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24; XTEN (32-aa linker)" /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24; XTEN" /label="32-aa linker" CDS 10285..12315 /note="Reverse transcriptase from Moloney murine leukemia virus. To be used for prime editing" /modified_by="mamee" /label="MoMLV-RT" NLS 12328..12378 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS BP*" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (NLS BP*)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; BP* (NLS BP* translation)" /Alternative_Match="100.00%; Adjusted-Interval-0:30/0; SV40 (NLS SV40)" /label="BP*" terminator 12386..12854 /created_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Pea3AT" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Pea3AT" /label="Pea3AT" promoter 12867..13876 /label="PromStUbiQ10" 5'UTR 13877..13975 intron 13976..14469 CDS join(14471..14473,14474..14476,14477..15190) /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITHGMDELYK" /label="EGFP" terminator 15195..15663 /created_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Pea3AT" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Pea3AT" /label="Pea3AT" misc_feature complement(15668..15678) /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" T-DNA_border 15685..15709 /note="right border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="RB T-DNA repeat" regulatory 17010..17639 /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" regulatory 18068..19140 /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" rep_origin 19206..19400 /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label="pVS1 oriV" regulatory 19774..20034 /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" rep_origin complement(20070..20658) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pColE1 ori" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pColE1 ori" /Alternative_Match="99.83%; Adjusted-Interval-0:0/0; pUC ori" /label="pColE1 ori" misc_feature complement(20746..21537) /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="99.75%" /Primary_Match="99.75%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" Promoter complement(21538..21812) /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="99.64%" /Primary_Match="99.64%; Adjusted-Interval-0:0/0; P1 (SpR)" /label="P1" T-DNA_border 22323..22347 /note="left border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="LB T-DNA repeat" ORIGIN 1 ggtctcagga gagcgatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg 61 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat 121 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta 181 attattacat gcttaacgta attcaacaga aattatatga taatcatggc aagaccggca 241 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag 301 ctagagtccg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggct 361 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg 421 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc 481 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc 541 ggcaagcagg cgtcgccgtg ggtcacgacg agatcctcgc cgtcgggcat ccgcgccttg 601 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga 661 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcggtgttt cgcttggtgg 721 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg 781 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc 841 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg 901 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt ggagttcatt cagggcaccg 961 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg 1021 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa 1081 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcctcgatc gagttgagag 1141 tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt 1201 tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg 1261 gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga gtggctcctt 1321 caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg 1381 gtcataacgt gactccctta attctcatgt atctccgtca ggagtttcac ttagttcagt 1441 tgcattatgt ctttatacac ccctacctag atgattaagt tttactttag ttggtgtgaa 1501 atggataaat tctaaaatat gaggtgtgga atgaaggatt gtcatcaatt agttggcccc 1561 aaccaagtaa aataagaagg ccggcccatt acaattaagt cgtcacacaa gtgggcttca 1621 ttgaaacaag cgcaaaaagg agtccaggcc cgtgttagcg tgaagactca accagcgatt 1681 tctccctcat cggttgcaca gaaaagctgt gtgttgttta tatggcgaaa cctaacagtc 1741 tgacttgttt gtcgagattt agcaatagtt taagagctat gctggaaaca gcatagcaag 1801 tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctctggtt 1861 tgataagagc attgctaaat ctctctctct cgttagctcg ccagttagcg aggtctgtcc 1921 ccacacgaca gataatcggg tgcaactccc gcccctcttc cgagggtcat cggaaccttt 1981 ttttgttttt tatgtctcgc tgtcaggagt ttcacttagt tcagttgcat tatgtcttta 2041 tacaccccta cctagatgat taagttttac tttagttggt gtgaaatgga taaattctaa 2101 aatatgaggt gtggaatgaa ggattgtcat caattagttg gccccaacca agtaaaataa 2161 gaaggccggc ccattacaat taagtcgtca cacaagtggg cttcattgaa acaagcgcaa 2221 aaaggagtcc aggcccgtgt tagcgtgaag actcaaccag cgatttctcc ctcatcggtt 2281 gcacagaaaa gctgtgtgtt gtttatatgg cgaaacctaa cagtctgact tgctaaatct 2341 cgacaaactg ctgtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag 2401 tccgttatca acttgaaaaa gtggcaccga gtcggtgcgg aggatcccca gtcagtttgt 2461 cgagtctctc tcgttagctc gccagttagc gaggtctgtc cccacacgac agataatcgg 2521 gtgcaactcc cgcccctctt ccgagggtca tcggaacctt tttttgtttt ttatgtctcg 2581 ctgtcaggag tttcacttag ttcagttgca ttatgtcttt atacacccct acctagatga 2641 ttaagtttta ctttagttgg tgtgaaatgg ataaattcta aaatatgagg tgtggaatga 2701 aggattgtca tcaattagtt ggccccaacc aagtaaaata agaaggccgg cccattacaa 2761 ttaagtcgtc acacaagtgg gcttcattga aacaagcgca aaaaggagtc caggcccgtg 2821 ttagcgtgaa gactcaacca gcgatttctc cctcatcggt tgcacagaaa agctgtgtgt 2881 tgtttatatg gcgaaaccta acagtctgac ttgtcaccca agcaagttgg ttagtttaag 2941 agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa 3001 agtggcaccg agtcggtgct taaaacaatg gaagtcagcc ctcattacca acttgcttct 3061 ctctcgttag ctcgccagtt agcgaggtct gtccccacac gacagataat cgggtgcaac 3121 tcccgcccct cttccgaggg tcatcggaac ctttttttgt tttttatgtc tcgctgtcag 3181 gagtttcact tagttcagtt gcattatgtc tttatacacc cctacctaga tgattaagtt 3241 ttactttagt tggtgtgaaa tggataaatt ctaaaatatg aggtgtggaa tgaaggattg 3301 tcatcaatta gttggcccca accaagtaaa ataagaaggc cggcccatta caattaagtc 3361 gtcacacaag tgggcttcat tgaaacaagc gcaaaaagga gtccaggccc gtgttagcgt 3421 gaagactcaa ccagcgattt ctccctcatc ggttgcacag aaaagctgtg tgttgtttat 3481 atggcgaaac ctaacagtct gacttgatca tggagatgaa atttggttta agagctatgc 3541 tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac 3601 cgagtcggtg cagaccggac cttaacgacc gcgccgcaaa tttcatctcc tctctctcgt 3661 tagctcgcca gttagcgagg tctgtcccca cacgacagat aatcgggtgc aactcccgcc 3721 cctcttccga gggtcatcgg aacctttttt tgttttttat gtctcgctgt caggagtttc 3781 acttagttca gttgcattat gtctttatac acccctacct agatgattaa gttttacttt 3841 agttggtgtg aaatggataa attctaaaat atgaggtgtg gaatgaagga ttgtcatcaa 3901 ttagttggcc ccaaccaagt aaaataagaa ggccggccca ttacaattaa gtcgtcacac 3961 aagtgggctt cattgaaaca agcgcaaaaa ggagtccagg cccgtgttag cgtgaagact 4021 caaccagcga tttctccctc atcggttgca cagaaaagct gtgtgttgtt tatatggcga 4081 aacctaacag tctgacttgt cacccaagca agttggttag tttaagagct atgctggaaa 4141 cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc 4201 ggtgcttaaa acaatggaag ccagcgccca ttaccaactt gcttctctct cgttagctcg 4261 ccagttagcg aggtctgtcc ccacacgaca gataatcggg tgcaactccc gcccctcttc 4321 cgagggtcat cggaaccttt ttttgttttt tatgtctcgc tgtcaggagc caagacaatt 4381 tcagcttaaa aagttttatt aatatttaca ttagttttgt tgatgaggat gacaagattt 4441 tggtcatcaa ttacatatac ccaaattgaa tacttagtaa gcaacttaat gtttttcata 4501 atgataatga cagacacaaa aaaaacccat ttattattca cattgattga tttttatatg 4561 cactatagta ataataataa tatttcttat aaagcaagag gtcaattttt tattttatta 4621 taccaacgac actaaattat atttgataat gtaaaacaat tcaattttac ttaaatatca 4681 tgaaataaac tatttttata accaaattac taaatttatc caataaaaaa aagtcattaa 4741 gaagacataa aataaatttg agtaaaaaga gtgaagtcga ctgacttttt tttttatcat 4801 aagaaaataa attattaact ttaacctaat aaaacactaa tataatttca tggaatctaa 4861 tacttacctc ttagatataa gaaaaagcgt ttctaataga ccctcaattt acattaaata 4921 ttttcaatca agtttaaata acaaatatca atatgaggtc aataacagta tcaaaataat 4981 atgaaaaaag agcaatacat aatataagaa agaagattta agtgcactta tcaaggtagt 5041 attatatcct aatttgctaa tatttaaact cttatattta aggtcatgtt cacgataaac 5101 ttgaaatgcg ctttattaga gcatatatta aaataaaaaa aatacctaaa ataaaataaa 5161 gttattttta gtatatattt tttacatgac ctacattttt ctagtttttt ctaaaggagc 5221 gtgtaagtgt caacctcatt ctcctaattt tccccaccac ataaaaatta aaaaggaaag 5281 gtagcttttg cgtgttgttt tggtacacta cacctcatta ttacacgtgt cctcatatag 5341 ttggttaacc cgtgaggcgg tttcctctag agtcggccat gccatctata aaatgaagct 5401 ttctgcacct caatttttca tcttctatct gatttctatt ataatttcta ttaattgcct 5461 tcaaatttct ctttcaaggt tagaaatctt ctctattttt tggtttttgt ctgtttagat 5521 tctcgaatta gctaatcagg tgctgttaaa gccctaaaat ttgagttttt tttccgtcga 5581 attgatgcta aaggcttaaa attagagttt tttcgtcggt ttgactctga aggcctaaaa 5641 tttggggttt tccgggtgat ttgatgataa agccctagaa tttgagtttt tttatttgtc 5701 ggtttgatga aaaaggcctt aaatttaatt tttttcccgg ttgatttgat gaaaaagccc 5761 tagaatttgt gttttttcgt cggtttgatt ctaaaggcct aaaatttgag tttttccggt 5821 tgttttgatg aaaaagccct aaaatttgag ttttttcccc gtgttttaga ttgtttggtt 5881 ttaattcttg aatcagataa tcagggagtg tgaaaagccc taaaatttga gtttttttcg 5941 ttgttctgat tgttgttttt atgaatttgc agaatgatga agagaactgc tgatggatct 6001 gaatttgaat ctcctaagaa gaagagaaag gttgataaga agtactctat cggactcgat 6061 atcggaacta actctgtggg atgggctgtg atcaccgatg agtacaaggt gccatctaag 6121 aagttcaagg ttctcggaaa caccgatagg cactctatca agaaaaacct tatcggtgct 6181 ctcctcttcg attctggtga aactgctgag gctaccagac tcaagagaac cgctagaaga 6241 aggtacacca gaagaaagaa caggatctgc tacctccaag agatcttctc taacgagatg 6301 gctaaagtgg atgattcatt cttccacagg ctcgaagagt cattcctcgt ggaagaagat 6361 aagaagcacg agaggcaccc tatcttcgga aacatcgttg atgaggtggc ataccacgag 6421 aagtacccta ctatctacca cctcagaaag aagctcgttg attctactga taaggctgat 6481 ctcaggctca tctacctcgc tctcgctcac atgatcaagt tcagaggaca cttcctcatc 6541 gagggtgatc tcaaccctga taactctgat gtggataagt tgttcatcca gctcgtgcag 6601 acctacaacc agcttttcga agagaaccct atcaacgctt caggtgtgga tgctaaggct 6661 atcctctctg ctaggctctc taagtcaaga aggcttgaga acctcattgc tcagctccct 6721 ggtgagaaga agaacggact tttcggaaac ttgatcgctc tctctctcgg actcacccct 6781 aacttcaagt ctaacttcga tctcgctgag gatgcaaagc tccagctctc aaaggatacc 6841 tacgatgatg atctcgataa cctcctcgct cagatcggag atcagtacgc tgatttgttc 6901 ctcgctgcta agaacctctc tgatgctatc ctcctcagtg atatcctcag agtgaacacc 6961 gagatcacca aggctccact ctcagcttct atgatcaaga gatacgatga gcaccaccag 7021 gatctcacac ttctcaaggc tcttgttaga cagcagctcc cagagaagta caaagagatt 7081 ttcttcgatc agtctaagaa cggatacgct ggttacatcg atggtggtgc atctcaagaa 7141 gagttctaca agttcatcaa gcctatcctc gagaagatgg atggaaccga ggaactcctc 7201 gtgaagctca atagagagga tcttctcaga aagcagagga ccttcgataa cggatctatc 7261 cctcatcaga tccacctcgg agagttgcac gctatcctta gaaggcaaga ggatttctac 7321 ccattcctca aggataacag ggaaaagatt gagaagattc tcaccttcag aatcccttac 7381 tacgtgggac ctctcgctag aggaaactca agattcgctt ggatgaccag aaagtctgag 7441 gaaaccatca ccccttggaa cttcgaagag gtggtggata agggtgctag tgctcagtct 7501 ttcatcgaga ggatgaccaa cttcgataag aaccttccaa acgagaaggt gctccctaag 7561 cactctttgc tctacgagta cttcaccgtg tacaacgagt tgaccaaggt taagtacgtg 7621 accgagggaa tgaggaagcc tgcttttttg tcaggtgagc aaaagaaggc tatcgttgat 7681 ctcttgttca agaccaacag aaaggtgacc gtgaagcagc tcaaagagga ttacttcaag 7741 aaaatcgagt gcttcgattc agttgagatt tctggtgttg aggataggtt caacgcatct 7801 ctcggaacct accacgatct cctcaagatc attaaggata aggatttctt ggataacgag 7861 gaaaacgagg atatcttgga ggatatcgtt cttaccctca ccctctttga agatagagag 7921 atgattgaag aaaggctcaa gacctacgct catctcttcg atgataaggt gatgaagcag 7981 ttgaagagaa gaagatacac tggttgggga aggctctcaa gaaagctcat taacggaatc 8041 agggataagc agtctggaaa gacaatcctt gatttcctca agtctgatgg attcgctaac 8101 agaaacttca tgcagctcat ccacgatgat tctctcacct ttaaagagga tatccagaag 8161 gctcaggttt caggacaggg tgatagtctc catgagcata tcgctaacct cgctggatct 8221 cctgcaatca agaagggaat cctccagact gtgaaggttg tggatgagtt ggtgaaggtg 8281 atgggaaggc ataagcctga gaacatcgtg atcgaaatgg ctagagagaa ccagaccact 8341 cagaagggac agaagaactc tagggaaagg atgaagagga tcgaggaagg tatcaaagag 8401 cttggatctc agatcctcaa agagcaccct gttgagaaca ctcagctcca gaatgagaag 8461 ctctacctct actacctcca gaacggaagg gatatgtatg tggatcaaga gttggatatc 8521 aacaggctct ctgattacga tgttgatgct atcgtgccac agtcattctt gaaggatgat 8581 tctatcgata acaaggtgct caccaggtct gataagaaca ggggtaagag tgataacgtg 8641 ccaagtgaag aggttgtgaa gaaaatgaag aactattgga ggcagctcct caacgctaag 8701 ctcatcactc agagaaagtt cgataacttg actaaggctg agaggggagg actctctgaa 8761 ttggataagg caggattcat caagaggcag cttgtggaaa ccaggcagat cactaagcac 8821 gttgcacaga tcctcgattc taggatgaac accaagtacg atgagaacga taagttgatc 8881 agggaagtga aggttatcac cctcaagtca aagctcgtgt ctgatttcag aaaggatttc 8941 caattctaca aggtgaggga aatcaacaac taccaccacg ctcacgatgc ttaccttaac 9001 gctgttgttg gaaccgctct catcaagaag tatcctaagc tcgagtcaga gttcgtgtac 9061 ggtgattaca aggtgtacga tgtgaggaag atgatcgcta agtctgagca agagatcgga 9121 aaggctaccg ctaagtattt cttctactct aacatcatga atttcttcaa gaccgagatt 9181 accctcgcta acggtgagat cagaaagagg ccactcatcg agacaaacgg tgaaacaggt 9241 gagatcgtgt gggataaggg aagggatttc gctaccgtta gaaaggtgct ctctatgcca 9301 caggtgaaca tcgttaagaa aaccgaggtg cagaccggtg gattctctaa agagtctatc 9361 ctccctaaga ggaactctga taagctcatt gctaggaaga aggattggga ccctaagaaa 9421 tacggtggtt tcgattctcc taccgtggct tactctgttc tcgttgtggc taaggttgag 9481 aagggaaaga gtaagaagct caagtctgtt aaggaacttc tcggaatcac tatcatggaa 9541 aggtcatctt tcgagaagaa cccaatcgat ttcctcgagg ctaagggata caaagaggtt 9601 aagaaggatc tcatcatcaa gctcccaaag tactcactct tcgaactcga gaacggtaga 9661 aagaggatgc tcgcttctgc tggtgagctt caaaagggaa acgagcttgc tctcccatct 9721 aagtacgtta actttcttta cctcgcttct cactacgaga agttgaaggg atctccagaa 9781 gataacgagc agaagcaact tttcgttgag cagcacaagc actacttgga tgagatcatc 9841 gagcagatct ctgagttctc taaaagggtg atcctcgctg atgcaaacct cgataaggtg 9901 ttgtctgctt acaacaagca cagagataag cctatcaggg aacaggcaga gaacatcatc 9961 catctcttca cccttaccaa cctcggtgct cctgctgctt tcaagtactt cgatacaacc 10021 atcgatagga agagatacac ctctaccaaa gaagtgctcg atgctaccct catccatcag 10081 tctatcactg gactctacga gactaggatc gatctctcac agctcggtgg tgattcgtca 10141 agggctgatg aattcccaaa gaagaagcgg aaggtggagc tcagcggagg atcttccgga 10201 ggatctagcg gctccgagac accaggaaca tccgaaagcg ctacaccaga atctagcgga 10261 ggctcttccg gaggatctag gcctaccctc aacatcgagg atgagtatcg cctccacgaa 10321 acctccaaag aaccggacgt gtccctcggc agcacatggc tcagcgactt cccacaagcg 10381 tgggccgaaa ccggcggcat gggcctcgcc gtccgccaag ccccactcat tatcccgctg 10441 aaggcgacct ccacaccggt gtccatcaag cagtacccga tgagccaaga ggcgaggctc 10501 gggattaagc cgcacattca gcgcctcctc gatcaaggca ttctcgtgcc gtgccaatcc 10561 ccgtggaata caccactcct cccggtcaaa aagccgggca ccaacgacta tcgcccggtc 10621 caagatctcc gcgaggtcaa caagcgcgtg gaagacatcc acccgaccgt cccgaacccg 10681 tataatctgc tctccgggct cccaccatcc caccagtggt atacagtgct ggacctcaaa 10741 gacgccttct tctgtctccg cctccaccca acaagccagc cgctcttcgc cttcgagtgg 10801 cgcgacccgg agatgggcat ctccggccaa ctgacatgga cacgcctccc gcaaggcttc 10861 aagaacagcc cgacactctt caacgaggcg ctccataggg acctcgcgga ttttcgcatc 10921 cagcatccgg acctcatcct cctccagtat gtggatgatc tcctcctcgc cgcgacctcc 10981 gagctggatt gtcaacaagg cacacgcgcg ctcctccaaa cactcgggaa cctcggctat 11041 cgcgcgtccg cgaaaaaggc ccaaatctgc cagaagcaag tgaagtacct cgggtatctg 11101 ctcaaggaag gccaacgctg gctcaccgaa gcgcgcaaag aaacagtgat ggggcaaccg 11161 acaccgaaaa caccacgcca gctgcgcgag tttctcggca aagccggctt ctgtcgcctc 11221 ttcatcccgg gctttgccga gatggccgcg ccactctacc cactcaccaa gccgggcaca 11281 ctgtttaact gggggccgga tcagcagaaa gcctaccaag agatcaaaca agcgctcctc 11341 accgccccag cgctcgggct cccagatctc acaaagccgt tcgagctgtt cgtcgatgag 11401 aagcaaggct acgcgaaggg cgtgctcaca cagaagctcg gcccgtggag gaggccagtg 11461 gcctatctct ccaaaaaact cgatccagtg gccgccggct ggccaccgtg tctgcgcatg 11521 gtcgccgcga ttgccgtgct cacaaaggat gccggcaaac tcacaatggg ccagccgctg 11581 gtgatcctcg cgccacatgc cgtggaagcc ctcgtcaaac agccgccgga taggtggctc 11641 tccaatgcgc gcatgaccca ttaccaagcg ctcctcctcg acaccgatcg cgtccagttc 11701 ggcccagtgg tcgccctcaa tccggcgaca ctgctgccac tcccagagga gggcctccaa 11761 cacaactgtc tggatattct cgcggaagcg catggcacaa ggccagacct cacagatcaa 11821 ccgctcccgg atgcggatca cacatggtat accgacggct cctctctgct ccaagagggc 11881 caaaggaaag ccggcgccgc ggtgaccaca gaaacagaag tgatctgggc caaggccctc 11941 ccagccggca catccgcgca aagggcggaa ctcatcgcgc tcacacaagc cctcaagatg 12001 gccgagggca agaagctcaa cgtctacaca gactcccgct atgccttcgc caccgcccac 12061 attcacggcg aaatctatag gaggcgcggc tggctcacaa gcgaggggaa ggagatcaag 12121 aacaaggatg agatcctcgc gctgctcaag gccctctttc tcccgaagcg cctctccatc 12181 atccactgtc cgggccacca aaaggggcac tccgcggaag cgaggggcaa taggatggcc 12241 gatcaagccg cgcgcaaagc cgcgattacc gaaaccccag acacatccac cctcctcatc 12301 gaaaactcct ccccatccgg cggcagcaag cgcacggcgg acggctcgga gttcgagccg 12361 aagaagaagc gcaaggtgtg agcttcaggc ctcccagctt tcgtccgtat catcggtttc 12421 gacaacgttc gtcaagttca atgcatcagt ttcattgccc acacaccaga atcctactaa 12481 gtttgagtat tatggcattg gaaaagctgt tttcttctat catttgttct gcttgtaatt 12541 tactgtgttc tttcagtttt tgttttcgga catcaaaatg caaatggatg gataagagtt 12601 aataaatgat atggtccttt tgttcattct caaattatta ttatctgttg tttttacttt 12661 aatgggttga atttaagtaa gaaaggaact aacagtgtga tattaaggtg caatgttaga 12721 catataaaac agtctttcac ctctctttgg ttatgtcttg aattggtttg tttcttcact 12781 tatctgtgta atcaagttta ctatgagtct atgatcaagt aattatgcaa tcaagttaag 12841 tacagtatag gcttcgctgt caggagccaa gacaatttca gcttaaaaag ttttattaat 12901 atttacatta gttttgttga tgaggatgac aagattttgg tcatcaatta catataccca 12961 aattgaatac ttagtaagca acttaatgtt tttcataatg ataatgacag acacaaaaaa 13021 aacccattta ttattcacat tgattgattt ttatatgcac tatagtaata ataataatat 13081 ttcttataaa gcaagaggtc aattttttat tttattatac caacgacact aaattatatt 13141 tgataatgta aaacaattca attttactta aatatcatga aataaactat ttttataacc 13201 aaattactaa atttatccaa taaaaaaaag tcattaagaa gacataaaat aaatttgagt 13261 aaaaagagtg aagtcgactg actttttttt ttatcataag aaaataaatt attaacttta 13321 acctaataaa acactaatat aatttcatgg aatctaatac ttacctctta gatataagaa 13381 aaagcgtttc taatagaccc tcaatttaca ttaaatattt tcaatcaagt ttaaataaca 13441 aatatcaata tgaggtcaat aacagtatca aaataatatg aaaaaagagc aatacataat 13501 ataagaaaga agatttaagt gcacttatca aggtagtatt atatcctaat ttgctaatat 13561 ttaaactctt atatttaagg tcatgttcac gataaacttg aaatgcgctt tattagagca 13621 tatattaaaa taaaaaaaat acctaaaata aaataaagtt atttttagta tatatttttt 13681 acatgaccta catttttcta gttttttcta aaggagcgtg taagtgtcaa cctcattctc 13741 ctaattttcc ccaccacata aaaattaaaa aggaaaggta gcttttgcgt gttgttttgg 13801 tacactacac ctcattatta cacgtgtcct catatagttg gttaacccgt gaggcggttt 13861 cctctagagt cggccatgcc atctataaaa tgaagctttc tgcacctcaa tttttcatct 13921 tctatctgat ttctattata atttctatta attgccttca aatttctctt tcaaggttag 13981 aaatcttctc tattttttgg tttttgtctg tttagattct cgaattagct aatcaggtgc 14041 tgttaaagcc ctaaaatttg agtttttttt ccgtcgaatt gatgctaaag gcttaaaatt 14101 agagtttttt cgtcggtttg actctgaagg cctaaaattt ggggttttcc gggtgatttg 14161 atgataaagc cctagaattt gagttttttt atttgtcggt ttgatgaaaa aggccttaaa 14221 tttaattttt ttcccggttg atttgatgaa aaagccctag aatttgtgtt ttttcgtcgg 14281 tttgattcta aaggcctaaa atttgagttt ttccggttgt tttgatgaaa aagccctaaa 14341 atttgagttt tttccccgtg ttttagattg tttggtttta attcttgaat cagataatca 14401 gggagtgtga aaagccctaa aatttgagtt tttttcgttg ttctgattgt tgtttttatg 14461 aatttgcaga atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 14521 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 14581 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 14641 ctggcccacc ctcgtgacca ccttcagcta cggcgtgcag tgcttcagcc gctaccccga 14701 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 14761 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 14821 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 14881 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 14941 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 15001 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 15061 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 15121 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct 15181 gtacaagtaa gcttcaggcc tcccagcttt cgtccgtatc atcggtttcg acaacgttcg 15241 tcaagttcaa tgcatcagtt tcattgccca cacaccagaa tcctactaag tttgagtatt 15301 atggcattgg aaaagctgtt ttcttctatc atttgttctg cttgtaattt actgtgttct 15361 ttcagttttt gttttcggac atcaaaatgc aaatggatgg ataagagtta ataaatgata 15421 tggtcctttt gttcattctc aaattattat tatctgttgt ttttacttta atgggttgaa 15481 tttaagtaag aaaggaacta acagtgtgat attaaggtgc aatgttagac atataaaaca 15541 gtctttcacc tctctttggt tatgtcttga attggtttgt ttcttcactt atctgtgtaa 15601 tcaagtttac tatgagtcta tgatcaagta attatgcaat caagttaagt acagtatagg 15661 cttcgctgtc atgagaccgg atcctgacag gatatattgg cgggtaaacc taagagaaaa 15721 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc 15781 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc 15841 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtcat ctgaaaacga 15901 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt 15961 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta 16021 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga 16081 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc 16141 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct 16201 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga 16261 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga 16321 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat 16381 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa 16441 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc 16501 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt 16561 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc 16621 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc 16681 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg 16741 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg 16801 cccgcgcacc tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag 16861 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg 16921 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga 16981 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag 17041 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg 17101 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc 17161 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga 17221 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg 17281 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg 17341 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg 17401 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg 17461 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc 17521 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg 17581 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag 17641 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag 17701 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg 17761 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca 17821 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag 17881 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag 17941 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag 18001 gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg 18061 aatcggcgtg acggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat 18121 gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa 18181 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa 18241 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat 18301 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg 18361 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt 18421 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac 18481 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg 18541 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc 18601 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac 18661 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta 18721 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg 18781 gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac 18841 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt 18901 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg 18961 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag 19021 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc 19081 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa 19141 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggactc 19201 tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg 19261 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata 19321 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa 19381 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg 19441 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc 19501 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc 19561 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg 19621 tgatgacggt gaaaacctct gacacatgca gctcccggtg acggtcacag cttgtctgta 19681 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 19741 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg 19801 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc 19861 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc 19921 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 19981 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 20041 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 20101 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 20161 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 20221 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 20281 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 20341 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 20401 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 20461 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 20521 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 20581 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 20641 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 20701 aacgaaaact cacgttaagg gattttggtc atgcattcta ggtgattatt tgccgactac 20761 cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc 20821 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata 20881 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc 20941 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcaccagc 21001 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg 21061 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc 21121 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc 21181 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg 21241 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct 21301 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc 21361 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc 21421 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat 21481 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcataat 21541 gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat 21601 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac 21661 cccaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg 21721 ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagcttacg 21781 aaccgaacag gcttatgtcc actgggttcg tgccttcatc cgtttccacg gtgtgcgtca 21841 cccggcaacc ttgggtagca gcgaagtcga ggcatttctg tcctggctgg aacagaactt 21901 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa 21961 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt 22021 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc 22081 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga 22141 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac 22201 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg 22261 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct 22321 ggtggcagga tatattgtgg tgtaaacata acggatcc //