LOCUS pDGB3_α2-T1T1d_StGFP_StPPE2 20618 bp DNA circular SYN 06-NOV-2024 DEFINITION a2_T1T1d_StGFP_StPPE2. ACCESSION VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid. REFERENCE 1 (bases 1 to 20618) AUTHORS SnapGene License TITLE Direct Submission JOURNAL Exported Oct 21, 2024 from SnapGene 7.2.1 https://www.snapgene.com FEATURES Location/Qualifiers misc_feature 1..11 /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" promoter 16..334 /modified_by="mamee" /label="StU6 promoter" misc_feature 338..358 /label="21bp Target" Scaffold 359..444 /modified_by="mamee" /label="esgRNA" misc_feature 445..463 /label="RT template" Change 456..458 /modified_by="mamee" /label="S to K" Change 459..461 /modified_by="mamee" /label="P to S" misc_feature 464..474 /label="PBS" Linker 475..482 /modified_by="mamee" /label="Peg-Linker" misc_feature 483..568 /note="TYMV TLS" terminator 569..588 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 601..919 /modified_by="mamee" /label="StU6 promoter" misc_feature 923..943 /label="21bp Target" Scaffold 944..1029 /modified_by="mamee" /label="esgRNA" misc_feature 1030..1044 /label="RT template" Change 1042..1044 /modified_by="mamee" /label="A to D" misc_feature 1045..1055 /label="PBS" Linker 1056..1063 /modified_by="mamee" /label="Peg-Linker" misc_feature 1064..1149 /note="TYMV TLS" terminator 1150..1169 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 1182..1500 /modified_by="mamee" /label="StU6 promoter" misc_feature 1505..1524 /label="20bp Target" Scaffold 1525..1610 /modified_by="mamee" /label="esgRNA" misc_feature 1611..1638 /label="RT template" Change 1622 /modified_by="mamee" /label="Silent SNP" Change 1628 /modified_by="mamee" /label="Silent SNP" Change 1631..1633 /modified_by="mamee" /label="G110R" Change 1637 /modified_by="mamee" /label="Silent SNP" misc_feature 1639..1648 /label="PBS" Linker 1649..1656 /modified_by="mamee" /label="Peg-Linker" misc_feature 1657..1742 /note="TYMV TLS" terminator 1743..1762 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 1775..2093 /modified_by="mamee" /label="StU6 promoter" misc_feature 2097..2116 /label="20bp Target" Scaffold 2117..2202 /modified_by="mamee" /label="esgRNA" misc_feature 2203..2230 /label="Rt template" Change 2212..2213 /modified_by="mamee" /label="S172K" Change 2221 /label="Silent SNP" Change 2224 /label="Silent SNP" Change 2227 /label="Silent SNP" misc_feature 2231..2241 /label="PBS" Linker 2242..2249 /modified_by="mamee" /label="Peg-Linker" misc_feature 2250..2335 /note="TYMV TLS" terminator 2336..2355 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 2368..2686 /modified_by="mamee" /label="StU6 promoter" misc_feature 2690..2710 /label="21bp Target" Scaffold 2711..2796 /modified_by="mamee" /label="esgRNA" misc_feature 2797..2825 /label="RT_Template" Change 2808..2813 /label="P73S-S72K" Change 2823 /label="Silent SNP" misc_feature 2826..2836 /label="PBS" Linker 2837..2844 /modified_by="mamee" /label="Peg-Linker" misc_feature 2845..2930 /note="TYMV TLS" terminator 2931..2950 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 2963..3972 /label="PromStUbiQ10" 5'UTR 3973..4071 intron 4072..4565 NLS 4573..4626 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS BP" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP (NLS BP)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; BP (NLS BP translation)" /label="BP" CDS 4627..8727 /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AtCas9-H840A" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AtCas9-H840A" /Alternative_Match="99.98%; Adjusted-Interval-0:0/0; AtCas9-D10A,H840A" /Alternative_Match="99.95%; Adjusted-Interval-0:0/0; AtCas9" /Alternative_Match="99.93%; Adjusted-Interval-0:0/0; AtCas9-D10A" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SpCas9(H840A)" /Alternative_Match="99.93%; Adjusted-Interval-0:0/0; SpCas9" /Alternative_Match="99.93%; Adjusted-Interval-0:0/0; SpCas9(D10A,H840A)" /Alternative_Match="99.85%; Adjusted-Interval-0:0/0; SpCas9(D10A)" /label="AtCas9-H840A" NLS 8749..8769 /created_by="warddecaestecker" /modified_by="wacae" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS SV40" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /label="SV40" Linker 8776..8871 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="32-aa linker" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 32-aa linker" /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24; XTEN (32-aa linker)" /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24; XTEN" /label="32-aa linker" CDS 8878..10908 /note="Reverse transcriptase from Moloney murine leukemia virus. To be used for prime editing" /modified_by="mamee" /label="MoMLV-RT" NLS 10921..10971 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS BP*" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (NLS BP*)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; BP* (NLS BP* translation)" /Alternative_Match="100.00%; Adjusted-Interval-0:30/0; SV40 (NLS SV40)" /label="BP*" terminator 10979..11447 /created_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Pea3AT" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Pea3AT" /label="Pea3AT" promoter 11460..12469 /label="PromStUbiQ10" 5'UTR 12470..12568 intron 12569..13062 CDS join(13064..13066,13067..13069,13070..13783) /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITHGMDELYK" /label="EGFP" terminator 13788..14256 /created_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Pea3AT" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Pea3AT" /label="Pea3AT" misc_feature complement(14257..14267) /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" T-DNA_border 14274..14298 /note="right border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="RB T-DNA repeat" regulatory 15599..16228 /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" regulatory 16657..17729 /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" rep_origin 17795..17989 /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label="pVS1 oriV" regulatory 18363..18623 /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" rep_origin complement(18659..19247) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pColE1 ori" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pColE1 ori" /Alternative_Match="99.83%; Adjusted-Interval-0:0/0; pUC ori" /label="pColE1 ori" misc_feature complement(19335..20150) /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="98.53%" /Primary_Match="98.53%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" T-DNA_border 20583..20607 /note="left border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="LB T-DNA repeat" ORIGIN 1 cgtctcagtc aggagtttca cttagttcag ttgcattatg tctttataca cccctaccta 61 gatgattaag ttttacttta gttggtgtga aatggataaa ttctaaaata tgaggtgtgg 121 aatgaaggat tgtcatcaat tagttggccc caaccaagta aaataagaag gccggcccat 181 tacaattaag tcgtcacaca agtgggcttc attgaaacaa gcgcaaaaag gagtccaggc 241 ccgtgttagc gtgaagactc aaccagcgat ttctccctca tcggttgcac agaaaagctg 301 tgtgttgttt atatggcgaa acctaacagt ctgacttgtt tgtcgagatt tagcaatagt 361 ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg ttatcaactt 421 gaaaaagtgg caccgagtcg gtgctctggt ttgataagag cattgctaaa tctctctctc 481 tcgttagctc gccagttagc gaggtctgtc cccacacgac agataatcgg gtgcaactcc 541 cgcccctctt ccgagggtca tcggaacctt tttttgtttt ttatgtctcg ctgtcaggag 601 tttcacttag ttcagttgca ttatgtcttt atacacccct acctagatga ttaagtttta 661 ctttagttgg tgtgaaatgg ataaattcta aaatatgagg tgtggaatga aggattgtca 721 tcaattagtt ggccccaacc aagtaaaata agaaggccgg cccattacaa ttaagtcgtc 781 acacaagtgg gcttcattga aacaagcgca aaaaggagtc caggcccgtg ttagcgtgaa 841 gactcaacca gcgatttctc cctcatcggt tgcacagaaa agctgtgtgt tgtttatatg 901 gcgaaaccta acagtctgac ttgctaaatc tcgacaaact gctgtttaag agctatgctg 961 gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa agtggcaccg 1021 agtcggtgcg gaggatcccc agtcagtttg tcgagtctct ctcgttagct cgccagttag 1081 cgaggtctgt ccccacacga cagataatcg ggtgcaactc ccgcccctct tccgagggtc 1141 atcggaacct ttttttgttt tttatgtctc gctgtcagga gtttcactta gttcagttgc 1201 attatgtctt tatacacccc tacctagatg attaagtttt actttagttg gtgtgaaatg 1261 gataaattct aaaatatgag gtgtggaatg aaggattgtc atcaattagt tggccccaac 1321 caagtaaaat aagaaggccg gcccattaca attaagtcgt cacacaagtg ggcttcattg 1381 aaacaagcgc aaaaaggagt ccaggcccgt gttagcgtga agactcaacc agcgatttct 1441 ccctcatcgg ttgcacagaa aagctgtgtg ttgtttatat ggcgaaacct aacagtctga 1501 cttgtcaccc aagcaagttg gttagtttaa gagctatgct ggaaacagca tagcaagttt 1561 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttaaaacaat 1621 ggaagtcagc cctcattacc aacttgcttc tctctcgtta gctcgccagt tagcgaggtc 1681 tgtccccaca cgacagataa tcgggtgcaa ctcccgcccc tcttccgagg gtcatcggaa 1741 cctttttttg ttttttatgt ctcgctgtca ggagtttcac ttagttcagt tgcattatgt 1801 ctttatacac ccctacctag atgattaagt tttactttag ttggtgtgaa atggataaat 1861 tctaaaatat gaggtgtgga atgaaggatt gtcatcaatt agttggcccc aaccaagtaa 1921 aataagaagg ccggcccatt acaattaagt cgtcacacaa gtgggcttca ttgaaacaag 1981 cgcaaaaagg agtccaggcc cgtgttagcg tgaagactca accagcgatt tctccctcat 2041 cggttgcaca gaaaagctgt gtgttgttta tatggcgaaa cctaacagtc tgacttgatc 2101 atggagatga aatttggttt aagagctatg ctggaaacag catagcaagt ttaaataagg 2161 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcagaccgga ccttaacgac 2221 cgcgccgcaa atttcatctc ctctctctcg ttagctcgcc agttagcgag gtctgtcccc 2281 acacgacaga taatcgggtg caactcccgc ccctcttccg agggtcatcg gaaccttttt 2341 ttgtttttta tgtctcgctg tcaggagttt cacttagttc agttgcatta tgtctttata 2401 cacccctacc tagatgatta agttttactt tagttggtgt gaaatggata aattctaaaa 2461 tatgaggtgt ggaatgaagg attgtcatca attagttggc cccaaccaag taaaataaga 2521 aggccggccc attacaatta agtcgtcaca caagtgggct tcattgaaac aagcgcaaaa 2581 aggagtccag gcccgtgtta gcgtgaagac tcaaccagcg atttctccct catcggttgc 2641 acagaaaagc tgtgtgttgt ttatatggcg aaacctaaca gtctgacttg tggagcattc 2701 atggactttt gtttaagagc tatgctggaa acagcatagc aagtttaaat aaggctagtc 2761 cgttatcaac ttgaaaaagt ggcaccgagt cggtgcgatt tagcaatgct cttatcaaac 2821 cagaaagtcc atgaattctc tctcgttagc tcgccagtta gcgaggtctg tccccacacg 2881 acagataatc gggtgcaact cccgcccctc ttccgagggt catcggaacc tttttttgtt 2941 ttttatgtct cgctgtcagg agccaagaca atttcagctt aaaaagtttt attaatattt 3001 acattagttt tgttgatgag gatgacaaga ttttggtcat caattacata tacccaaatt 3061 gaatacttag taagcaactt aatgtttttc ataatgataa tgacagacac aaaaaaaacc 3121 catttattat tcacattgat tgatttttat atgcactata gtaataataa taatatttct 3181 tataaagcaa gaggtcaatt ttttatttta ttataccaac gacactaaat tatatttgat 3241 aatgtaaaac aattcaattt tacttaaata tcatgaaata aactattttt ataaccaaat 3301 tactaaattt atccaataaa aaaaagtcat taagaagaca taaaataaat ttgagtaaaa 3361 agagtgaagt cgactgactt ttttttttat cataagaaaa taaattatta actttaacct 3421 aataaaacac taatataatt tcatggaatc taatacttac ctcttagata taagaaaaag 3481 cgtttctaat agaccctcaa tttacattaa atattttcaa tcaagtttaa ataacaaata 3541 tcaatatgag gtcaataaca gtatcaaaat aatatgaaaa aagagcaata cataatataa 3601 gaaagaagat ttaagtgcac ttatcaaggt agtattatat cctaatttgc taatatttaa 3661 actcttatat ttaaggtcat gttcacgata aacttgaaat gcgctttatt agagcatata 3721 ttaaaataaa aaaaatacct aaaataaaat aaagttattt ttagtatata ttttttacat 3781 gacctacatt tttctagttt tttctaaagg agcgtgtaag tgtcaacctc attctcctaa 3841 ttttccccac cacataaaaa ttaaaaagga aaggtagctt ttgcgtgttg ttttggtaca 3901 ctacacctca ttattacacg tgtcctcata tagttggtta acccgtgagg cggtttcctc 3961 tagagtcggc catgccatct ataaaatgaa gctttctgca cctcaatttt tcatcttcta 4021 tctgatttct attataattt ctattaattg ccttcaaatt tctctttcaa ggttagaaat 4081 cttctctatt ttttggtttt tgtctgttta gattctcgaa ttagctaatc aggtgctgtt 4141 aaagccctaa aatttgagtt ttttttccgt cgaattgatg ctaaaggctt aaaattagag 4201 ttttttcgtc ggtttgactc tgaaggccta aaatttgggg ttttccgggt gatttgatga 4261 taaagcccta gaatttgagt ttttttattt gtcggtttga tgaaaaaggc cttaaattta 4321 atttttttcc cggttgattt gatgaaaaag ccctagaatt tgtgtttttt cgtcggtttg 4381 attctaaagg cctaaaattt gagtttttcc ggttgttttg atgaaaaagc cctaaaattt 4441 gagttttttc cccgtgtttt agattgtttg gttttaattc ttgaatcaga taatcaggga 4501 gtgtgaaaag ccctaaaatt tgagtttttt tcgttgttct gattgttgtt tttatgaatt 4561 tgcagaatga tgaagagaac tgctgatgga tctgaatttg aatctcctaa gaagaagaga 4621 aaggttgata agaagtactc tatcggactc gatatcggaa ctaactctgt gggatgggct 4681 gtgatcaccg atgagtacaa ggtgccatct aagaagttca aggttctcgg aaacaccgat 4741 aggcactcta tcaagaaaaa ccttatcggt gctctcctct tcgattctgg tgaaactgct 4801 gaggctacca gactcaagag aaccgctaga agaaggtaca ccagaagaaa gaacaggatc 4861 tgctacctcc aagagatctt ctctaacgag atggctaaag tggatgattc attcttccac 4921 aggctcgaag agtcattcct cgtggaagaa gataagaagc acgagaggca ccctatcttc 4981 ggaaacatcg ttgatgaggt ggcataccac gagaagtacc ctactatcta ccacctcaga 5041 aagaagctcg ttgattctac tgataaggct gatctcaggc tcatctacct cgctctcgct 5101 cacatgatca agttcagagg acacttcctc atcgagggtg atctcaaccc tgataactct 5161 gatgtggata agttgttcat ccagctcgtg cagacctaca accagctttt cgaagagaac 5221 cctatcaacg cttcaggtgt ggatgctaag gctatcctct ctgctaggct ctctaagtca 5281 agaaggcttg agaacctcat tgctcagctc cctggtgaga agaagaacgg acttttcgga 5341 aacttgatcg ctctctctct cggactcacc cctaacttca agtctaactt cgatctcgct 5401 gaggatgcaa agctccagct ctcaaaggat acctacgatg atgatctcga taacctcctc 5461 gctcagatcg gagatcagta cgctgatttg ttcctcgctg ctaagaacct ctctgatgct 5521 atcctcctca gtgatatcct cagagtgaac accgagatca ccaaggctcc actctcagct 5581 tctatgatca agagatacga tgagcaccac caggatctca cacttctcaa ggctcttgtt 5641 agacagcagc tcccagagaa gtacaaagag attttcttcg atcagtctaa gaacggatac 5701 gctggttaca tcgatggtgg tgcatctcaa gaagagttct acaagttcat caagcctatc 5761 ctcgagaaga tggatggaac cgaggaactc ctcgtgaagc tcaatagaga ggatcttctc 5821 agaaagcaga ggaccttcga taacggatct atccctcatc agatccacct cggagagttg 5881 cacgctatcc ttagaaggca agaggatttc tacccattcc tcaaggataa cagggaaaag 5941 attgagaaga ttctcacctt cagaatccct tactacgtgg gacctctcgc tagaggaaac 6001 tcaagattcg cttggatgac cagaaagtct gaggaaacca tcaccccttg gaacttcgaa 6061 gaggtggtgg ataagggtgc tagtgctcag tctttcatcg agaggatgac caacttcgat 6121 aagaaccttc caaacgagaa ggtgctccct aagcactctt tgctctacga gtacttcacc 6181 gtgtacaacg agttgaccaa ggttaagtac gtgaccgagg gaatgaggaa gcctgctttt 6241 ttgtcaggtg agcaaaagaa ggctatcgtt gatctcttgt tcaagaccaa cagaaaggtg 6301 accgtgaagc agctcaaaga ggattacttc aagaaaatcg agtgcttcga ttcagttgag 6361 atttctggtg ttgaggatag gttcaacgca tctctcggaa cctaccacga tctcctcaag 6421 atcattaagg ataaggattt cttggataac gaggaaaacg aggatatctt ggaggatatc 6481 gttcttaccc tcaccctctt tgaagataga gagatgattg aagaaaggct caagacctac 6541 gctcatctct tcgatgataa ggtgatgaag cagttgaaga gaagaagata cactggttgg 6601 ggaaggctct caagaaagct cattaacgga atcagggata agcagtctgg aaagacaatc 6661 cttgatttcc tcaagtctga tggattcgct aacagaaact tcatgcagct catccacgat 6721 gattctctca cctttaaaga ggatatccag aaggctcagg tttcaggaca gggtgatagt 6781 ctccatgagc atatcgctaa cctcgctgga tctcctgcaa tcaagaaggg aatcctccag 6841 actgtgaagg ttgtggatga gttggtgaag gtgatgggaa ggcataagcc tgagaacatc 6901 gtgatcgaaa tggctagaga gaaccagacc actcagaagg gacagaagaa ctctagggaa 6961 aggatgaaga ggatcgagga aggtatcaaa gagcttggat ctcagatcct caaagagcac 7021 cctgttgaga acactcagct ccagaatgag aagctctacc tctactacct ccagaacgga 7081 agggatatgt atgtggatca agagttggat atcaacaggc tctctgatta cgatgttgat 7141 gctatcgtgc cacagtcatt cttgaaggat gattctatcg ataacaaggt gctcaccagg 7201 tctgataaga acaggggtaa gagtgataac gtgccaagtg aagaggttgt gaagaaaatg 7261 aagaactatt ggaggcagct cctcaacgct aagctcatca ctcagagaaa gttcgataac 7321 ttgactaagg ctgagagggg aggactctct gaattggata aggcaggatt catcaagagg 7381 cagcttgtgg aaaccaggca gatcactaag cacgttgcac agatcctcga ttctaggatg 7441 aacaccaagt acgatgagaa cgataagttg atcagggaag tgaaggttat caccctcaag 7501 tcaaagctcg tgtctgattt cagaaaggat ttccaattct acaaggtgag ggaaatcaac 7561 aactaccacc acgctcacga tgcttacctt aacgctgttg ttggaaccgc tctcatcaag 7621 aagtatccta agctcgagtc agagttcgtg tacggtgatt acaaggtgta cgatgtgagg 7681 aagatgatcg ctaagtctga gcaagagatc ggaaaggcta ccgctaagta tttcttctac 7741 tctaacatca tgaatttctt caagaccgag attaccctcg ctaacggtga gatcagaaag 7801 aggccactca tcgagacaaa cggtgaaaca ggtgagatcg tgtgggataa gggaagggat 7861 ttcgctaccg ttagaaaggt gctctctatg ccacaggtga acatcgttaa gaaaaccgag 7921 gtgcagaccg gtggattctc taaagagtct atcctcccta agaggaactc tgataagctc 7981 attgctagga agaaggattg ggaccctaag aaatacggtg gtttcgattc tcctaccgtg 8041 gcttactctg ttctcgttgt ggctaaggtt gagaagggaa agagtaagaa gctcaagtct 8101 gttaaggaac ttctcggaat cactatcatg gaaaggtcat ctttcgagaa gaacccaatc 8161 gatttcctcg aggctaaggg atacaaagag gttaagaagg atctcatcat caagctccca 8221 aagtactcac tcttcgaact cgagaacggt agaaagagga tgctcgcttc tgctggtgag 8281 cttcaaaagg gaaacgagct tgctctccca tctaagtacg ttaactttct ttacctcgct 8341 tctcactacg agaagttgaa gggatctcca gaagataacg agcagaagca acttttcgtt 8401 gagcagcaca agcactactt ggatgagatc atcgagcaga tctctgagtt ctctaaaagg 8461 gtgatcctcg ctgatgcaaa cctcgataag gtgttgtctg cttacaacaa gcacagagat 8521 aagcctatca gggaacaggc agagaacatc atccatctct tcacccttac caacctcggt 8581 gctcctgctg ctttcaagta cttcgataca accatcgata ggaagagata cacctctacc 8641 aaagaagtgc tcgatgctac cctcatccat cagtctatca ctggactcta cgagactagg 8701 atcgatctct cacagctcgg tggtgattcg tcaagggctg atgaattccc aaagaagaag 8761 cggaaggtgg agctcagcgg aggatcttcc ggaggatcta gcggctccga gacaccagga 8821 acatccgaaa gcgctacacc agaatctagc ggaggctctt ccggaggatc taggcctacc 8881 ctcaacatcg aggatgagta tcgcctccac gaaacctcca aagaaccgga cgtgtccctc 8941 ggcagcacat ggctcagcga cttcccacaa gcgtgggccg aaaccggcgg catgggcctc 9001 gccgtccgcc aagccccact cattatcccg ctgaaggcga cctccacacc ggtgtccatc 9061 aagcagtacc cgatgagcca agaggcgagg ctcgggatta agccgcacat tcagcgcctc 9121 ctcgatcaag gcattctcgt gccgtgccaa tccccgtgga atacaccact cctcccggtc 9181 aaaaagccgg gcaccaacga ctatcgcccg gtccaagatc tccgcgaggt caacaagcgc 9241 gtggaagaca tccacccgac cgtcccgaac ccgtataatc tgctctccgg gctcccacca 9301 tcccaccagt ggtatacagt gctggacctc aaagacgcct tcttctgtct ccgcctccac 9361 ccaacaagcc agccgctctt cgccttcgag tggcgcgacc cggagatggg catctccggc 9421 caactgacat ggacacgcct cccgcaaggc ttcaagaaca gcccgacact cttcaacgag 9481 gcgctccata gggacctcgc ggattttcgc atccagcatc cggacctcat cctcctccag 9541 tatgtggatg atctcctcct cgccgcgacc tccgagctgg attgtcaaca aggcacacgc 9601 gcgctcctcc aaacactcgg gaacctcggc tatcgcgcgt ccgcgaaaaa ggcccaaatc 9661 tgccagaagc aagtgaagta cctcgggtat ctgctcaagg aaggccaacg ctggctcacc 9721 gaagcgcgca aagaaacagt gatggggcaa ccgacaccga aaacaccacg ccagctgcgc 9781 gagtttctcg gcaaagccgg cttctgtcgc ctcttcatcc cgggctttgc cgagatggcc 9841 gcgccactct acccactcac caagccgggc acactgttta actgggggcc ggatcagcag 9901 aaagcctacc aagagatcaa acaagcgctc ctcaccgccc cagcgctcgg gctcccagat 9961 ctcacaaagc cgttcgagct gttcgtcgat gagaagcaag gctacgcgaa gggcgtgctc 10021 acacagaagc tcggcccgtg gaggaggcca gtggcctatc tctccaaaaa actcgatcca 10081 gtggccgccg gctggccacc gtgtctgcgc atggtcgccg cgattgccgt gctcacaaag 10141 gatgccggca aactcacaat gggccagccg ctggtgatcc tcgcgccaca tgccgtggaa 10201 gccctcgtca aacagccgcc ggataggtgg ctctccaatg cgcgcatgac ccattaccaa 10261 gcgctcctcc tcgacaccga tcgcgtccag ttcggcccag tggtcgccct caatccggcg 10321 acactgctgc cactcccaga ggagggcctc caacacaact gtctggatat tctcgcggaa 10381 gcgcatggca caaggccaga cctcacagat caaccgctcc cggatgcgga tcacacatgg 10441 tataccgacg gctcctctct gctccaagag ggccaaagga aagccggcgc cgcggtgacc 10501 acagaaacag aagtgatctg ggccaaggcc ctcccagccg gcacatccgc gcaaagggcg 10561 gaactcatcg cgctcacaca agccctcaag atggccgagg gcaagaagct caacgtctac 10621 acagactccc gctatgcctt cgccaccgcc cacattcacg gcgaaatcta taggaggcgc 10681 ggctggctca caagcgaggg gaaggagatc aagaacaagg atgagatcct cgcgctgctc 10741 aaggccctct ttctcccgaa gcgcctctcc atcatccact gtccgggcca ccaaaagggg 10801 cactccgcgg aagcgagggg caataggatg gccgatcaag ccgcgcgcaa agccgcgatt 10861 accgaaaccc cagacacatc caccctcctc atcgaaaact cctccccatc cggcggcagc 10921 aagcgcacgg cggacggctc ggagttcgag ccgaagaaga agcgcaaggt gtgagcttca 10981 ggcctcccag ctttcgtccg tatcatcggt ttcgacaacg ttcgtcaagt tcaatgcatc 11041 agtttcattg cccacacacc agaatcctac taagtttgag tattatggca ttggaaaagc 11101 tgttttcttc tatcatttgt tctgcttgta atttactgtg ttctttcagt ttttgttttc 11161 ggacatcaaa atgcaaatgg atggataaga gttaataaat gatatggtcc ttttgttcat 11221 tctcaaatta ttattatctg ttgtttttac tttaatgggt tgaatttaag taagaaagga 11281 actaacagtg tgatattaag gtgcaatgtt agacatataa aacagtcttt cacctctctt 11341 tggttatgtc ttgaattggt ttgtttcttc acttatctgt gtaatcaagt ttactatgag 11401 tctatgatca agtaattatg caatcaagtt aagtacagta taggcttcgc tgtcaggagc 11461 caagacaatt tcagcttaaa aagttttatt aatatttaca ttagttttgt tgatgaggat 11521 gacaagattt tggtcatcaa ttacatatac ccaaattgaa tacttagtaa gcaacttaat 11581 gtttttcata atgataatga cagacacaaa aaaaacccat ttattattca cattgattga 11641 tttttatatg cactatagta ataataataa tatttcttat aaagcaagag gtcaattttt 11701 tattttatta taccaacgac actaaattat atttgataat gtaaaacaat tcaattttac 11761 ttaaatatca tgaaataaac tatttttata accaaattac taaatttatc caataaaaaa 11821 aagtcattaa gaagacataa aataaatttg agtaaaaaga gtgaagtcga ctgacttttt 11881 tttttatcat aagaaaataa attattaact ttaacctaat aaaacactaa tataatttca 11941 tggaatctaa tacttacctc ttagatataa gaaaaagcgt ttctaataga ccctcaattt 12001 acattaaata ttttcaatca agtttaaata acaaatatca atatgaggtc aataacagta 12061 tcaaaataat atgaaaaaag agcaatacat aatataagaa agaagattta agtgcactta 12121 tcaaggtagt attatatcct aatttgctaa tatttaaact cttatattta aggtcatgtt 12181 cacgataaac ttgaaatgcg ctttattaga gcatatatta aaataaaaaa aatacctaaa 12241 ataaaataaa gttattttta gtatatattt tttacatgac ctacattttt ctagtttttt 12301 ctaaaggagc gtgtaagtgt caacctcatt ctcctaattt tccccaccac ataaaaatta 12361 aaaaggaaag gtagcttttg cgtgttgttt tggtacacta cacctcatta ttacacgtgt 12421 cctcatatag ttggttaacc cgtgaggcgg tttcctctag agtcggccat gccatctata 12481 aaatgaagct ttctgcacct caatttttca tcttctatct gatttctatt ataatttcta 12541 ttaattgcct tcaaatttct ctttcaaggt tagaaatctt ctctattttt tggtttttgt 12601 ctgtttagat tctcgaatta gctaatcagg tgctgttaaa gccctaaaat ttgagttttt 12661 tttccgtcga attgatgcta aaggcttaaa attagagttt tttcgtcggt ttgactctga 12721 aggcctaaaa tttggggttt tccgggtgat ttgatgataa agccctagaa tttgagtttt 12781 tttatttgtc ggtttgatga aaaaggcctt aaatttaatt tttttcccgg ttgatttgat 12841 gaaaaagccc tagaatttgt gttttttcgt cggtttgatt ctaaaggcct aaaatttgag 12901 tttttccggt tgttttgatg aaaaagccct aaaatttgag ttttttcccc gtgttttaga 12961 ttgtttggtt ttaattcttg aatcagataa tcagggagtg tgaaaagccc taaaatttga 13021 gtttttttcg ttgttctgat tgttgttttt atgaatttgc agaatggtga gcaagggcga 13081 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 13141 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 13201 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcag 13261 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 13321 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 13381 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 13441 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 13501 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 13561 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 13621 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 13681 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 13741 cgccgccggg atcactcacg gcatggacga gctgtacaag taagcttcag gcctcccagc 13801 tttcgtccgt atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc 13861 ccacacacca gaatcctact aagtttgagt attatggcat tggaaaagct gttttcttct 13921 atcatttgtt ctgcttgtaa tttactgtgt tctttcagtt tttgttttcg gacatcaaaa 13981 tgcaaatgga tggataagag ttaataaatg atatggtcct tttgttcatt ctcaaattat 14041 tattatctgt tgtttttact ttaatgggtt gaatttaagt aagaaaggaa ctaacagtgt 14101 gatattaagg tgcaatgtta gacatataaa acagtctttc acctctcttt ggttatgtct 14161 tgaattggtt tgtttcttca cttatctgtg taatcaagtt tactatgagt ctatgatcaa 14221 gtaattatgc aatcaagtta agtacagtat aggcttcgct tgagacgaag ctttgacagg 14281 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa 14341 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc 14401 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga 14461 cgttcagtgc agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag 14521 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag 14581 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg 14641 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg 14701 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc 14761 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg 14821 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag 14881 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc 14941 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac 15001 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct 15061 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc 15121 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag 15181 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca 15241 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga 15301 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga 15361 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg cggctgcatg 15421 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg 15481 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc 15541 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat 15601 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca 15661 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca 15721 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat 15781 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat 15841 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt 15901 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct 15961 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg 16021 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct 16081 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg 16141 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc 16201 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc 16261 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc 16321 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac 16381 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa 16441 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc 16501 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc 16561 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg 16621 caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc 16681 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg 16741 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg 16801 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga 16861 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca 16921 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac 16981 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc 17041 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa 17101 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc 17161 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg 17221 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga 17281 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca 17341 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga 17401 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact 17461 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca 17521 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt 17581 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg 17641 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga 17701 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc 17761 tagcagggga aaaaggtcga aaaggactct ttcctgtgga tagcacgtac attgggaacc 17821 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga 17881 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa 17941 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc 18001 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc 18061 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc 18121 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac 18181 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 18241 ctcccggtga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 18301 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat 18361 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc 18421 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 18481 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 18541 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 18601 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 18661 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 18721 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 18781 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 18841 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 18901 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 18961 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 19021 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 19081 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 19141 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 19201 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 19261 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 19321 tgcattctag gtgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata 19381 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca 19441 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca 19501 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca 19561 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact 19621 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta 19681 ttcattcgtg attgcgcctg agcgagtcga aatacgcgat cgctgttaaa aggacaatta 19741 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca 19801 cctgaatcag gatattcttc taatacctgg aatgctgttt tccctgggat cgcagtggtg 19861 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat 19921 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg 19981 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcggta gattgtcgca 20041 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg 20101 gaatttaatc gcggccttga gcaagacgtt tcccgttgaa tatggctcat aacagaactt 20161 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa 20221 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt 20281 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc 20341 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga 20401 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac 20461 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg 20521 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct 20581 ggtggcagga tatattgtgg tgtaaacata acaagctt //