LOCUS pDGB3_α2-StUBI-PPE2-tRbcS3a_4 14862 bp DNA circular SYN 06-NOV-2024 DEFINITION a2_StUBI-PPE2-tS3a_4. ACCESSION VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid. REFERENCE 1 (bases 1 to 14862) AUTHORS SnapGene License TITLE Direct Submission JOURNAL Exported Oct 21, 2024 from SnapGene 7.2.1 https://www.snapgene.com FEATURES Location/Qualifiers misc_feature 1..11 /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" promoter 16..1025 /label="PromStUbiQ10" 5'UTR 1026..1124 intron 1125..1618 NLS 1626..1679 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS BP" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP (NLS BP)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; BP (NLS BP translation)" /label="BP" CDS 1680..5780 /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AtCas9-H840A" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AtCas9-H840A" /Alternative_Match="99.98%; Adjusted-Interval-0:0/0; AtCas9-D10A,H840A" /Alternative_Match="99.95%; Adjusted-Interval-0:0/0; AtCas9" /Alternative_Match="99.93%; Adjusted-Interval-0:0/0; AtCas9-D10A" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SpCas9(H840A)" /Alternative_Match="99.93%; Adjusted-Interval-0:0/0; SpCas9" /Alternative_Match="99.93%; Adjusted-Interval-0:0/0; SpCas9(D10A,H840A)" /Alternative_Match="99.85%; Adjusted-Interval-0:0/0; SpCas9(D10A)" /label="AtCas9-H840A" NLS 5802..5822 /created_by="warddecaestecker" /modified_by="wacae" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS SV40" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /label="SV40" Linker 5829..5924 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="32-aa linker" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 32-aa linker" /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24; XTEN (32-aa linker)" /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24; XTEN" /label="32-aa linker" CDS 5931..7961 /note="Reverse transcriptase from Moloney murine leukemia virus. To be used for prime editing" /modified_by="mamee" /label="MoMLV-RT" NLS 7974..8024 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS BP*" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (NLS BP*)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; BP* (NLS BP* translation)" /Alternative_Match="100.00%; Adjusted-Interval-0:30/0; SV40 (NLS SV40)" /label="BP*" terminator 8032..8500 /created_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Pea3AT" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Pea3AT" /label="Pea3AT" misc_feature complement(8501..8511) /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" T-DNA_border 8518..8542 /note="right border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="RB T-DNA repeat" regulatory 9843..10472 /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" regulatory 10901..11973 /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" rep_origin 12039..12233 /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label="pVS1 oriV" regulatory 12607..12867 /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" rep_origin complement(12903..13491) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pColE1 ori" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pColE1 ori" /Alternative_Match="99.83%; Adjusted-Interval-0:0/0; pUC ori" /label="pColE1 ori" misc_feature complement(13579..14394) /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="98.53%" /Primary_Match="98.53%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" T-DNA_border 14827..14851 /note="left border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="LB T-DNA repeat" ORIGIN 1 cgtctcagtc aggagccaag acaatttcag cttaaaaagt tttattaata tttacattag 61 ttttgttgat gaggatgaca agattttggt catcaattac atatacccaa attgaatact 121 tagtaagcaa cttaatgttt ttcataatga taatgacaga cacaaaaaaa acccatttat 181 tattcacatt gattgatttt tatatgcact atagtaataa taataatatt tcttataaag 241 caagaggtca attttttatt ttattatacc aacgacacta aattatattt gataatgtaa 301 aacaattcaa ttttacttaa atatcatgaa ataaactatt tttataacca aattactaaa 361 tttatccaat aaaaaaaagt cattaagaag acataaaata aatttgagta aaaagagtga 421 agtcgactga cttttttttt tatcataaga aaataaatta ttaactttaa cctaataaaa 481 cactaatata atttcatgga atctaatact tacctcttag atataagaaa aagcgtttct 541 aatagaccct caatttacat taaatatttt caatcaagtt taaataacaa atatcaatat 601 gaggtcaata acagtatcaa aataatatga aaaaagagca atacataata taagaaagaa 661 gatttaagtg cacttatcaa ggtagtatta tatcctaatt tgctaatatt taaactctta 721 tatttaaggt catgttcacg ataaacttga aatgcgcttt attagagcat atattaaaat 781 aaaaaaaata cctaaaataa aataaagtta tttttagtat atatttttta catgacctac 841 atttttctag ttttttctaa aggagcgtgt aagtgtcaac ctcattctcc taattttccc 901 caccacataa aaattaaaaa ggaaaggtag cttttgcgtg ttgttttggt acactacacc 961 tcattattac acgtgtcctc atatagttgg ttaacccgtg aggcggtttc ctctagagtc 1021 ggccatgcca tctataaaat gaagctttct gcacctcaat ttttcatctt ctatctgatt 1081 tctattataa tttctattaa ttgccttcaa atttctcttt caaggttaga aatcttctct 1141 attttttggt ttttgtctgt ttagattctc gaattagcta atcaggtgct gttaaagccc 1201 taaaatttga gttttttttc cgtcgaattg atgctaaagg cttaaaatta gagttttttc 1261 gtcggtttga ctctgaaggc ctaaaatttg gggttttccg ggtgatttga tgataaagcc 1321 ctagaatttg agttttttta tttgtcggtt tgatgaaaaa ggccttaaat ttaatttttt 1381 tcccggttga tttgatgaaa aagccctaga atttgtgttt tttcgtcggt ttgattctaa 1441 aggcctaaaa tttgagtttt tccggttgtt ttgatgaaaa agccctaaaa tttgagtttt 1501 ttccccgtgt tttagattgt ttggttttaa ttcttgaatc agataatcag ggagtgtgaa 1561 aagccctaaa atttgagttt ttttcgttgt tctgattgtt gtttttatga atttgcagaa 1621 tgatgaagag aactgctgat ggatctgaat ttgaatctcc taagaagaag agaaaggttg 1681 ataagaagta ctctatcgga ctcgatatcg gaactaactc tgtgggatgg gctgtgatca 1741 ccgatgagta caaggtgcca tctaagaagt tcaaggttct cggaaacacc gataggcact 1801 ctatcaagaa aaaccttatc ggtgctctcc tcttcgattc tggtgaaact gctgaggcta 1861 ccagactcaa gagaaccgct agaagaaggt acaccagaag aaagaacagg atctgctacc 1921 tccaagagat cttctctaac gagatggcta aagtggatga ttcattcttc cacaggctcg 1981 aagagtcatt cctcgtggaa gaagataaga agcacgagag gcaccctatc ttcggaaaca 2041 tcgttgatga ggtggcatac cacgagaagt accctactat ctaccacctc agaaagaagc 2101 tcgttgattc tactgataag gctgatctca ggctcatcta cctcgctctc gctcacatga 2161 tcaagttcag aggacacttc ctcatcgagg gtgatctcaa ccctgataac tctgatgtgg 2221 ataagttgtt catccagctc gtgcagacct acaaccagct tttcgaagag aaccctatca 2281 acgcttcagg tgtggatgct aaggctatcc tctctgctag gctctctaag tcaagaaggc 2341 ttgagaacct cattgctcag ctccctggtg agaagaagaa cggacttttc ggaaacttga 2401 tcgctctctc tctcggactc acccctaact tcaagtctaa cttcgatctc gctgaggatg 2461 caaagctcca gctctcaaag gatacctacg atgatgatct cgataacctc ctcgctcaga 2521 tcggagatca gtacgctgat ttgttcctcg ctgctaagaa cctctctgat gctatcctcc 2581 tcagtgatat cctcagagtg aacaccgaga tcaccaaggc tccactctca gcttctatga 2641 tcaagagata cgatgagcac caccaggatc tcacacttct caaggctctt gttagacagc 2701 agctcccaga gaagtacaaa gagattttct tcgatcagtc taagaacgga tacgctggtt 2761 acatcgatgg tggtgcatct caagaagagt tctacaagtt catcaagcct atcctcgaga 2821 agatggatgg aaccgaggaa ctcctcgtga agctcaatag agaggatctt ctcagaaagc 2881 agaggacctt cgataacgga tctatccctc atcagatcca cctcggagag ttgcacgcta 2941 tccttagaag gcaagaggat ttctacccat tcctcaagga taacagggaa aagattgaga 3001 agattctcac cttcagaatc ccttactacg tgggacctct cgctagagga aactcaagat 3061 tcgcttggat gaccagaaag tctgaggaaa ccatcacccc ttggaacttc gaagaggtgg 3121 tggataaggg tgctagtgct cagtctttca tcgagaggat gaccaacttc gataagaacc 3181 ttccaaacga gaaggtgctc cctaagcact ctttgctcta cgagtacttc accgtgtaca 3241 acgagttgac caaggttaag tacgtgaccg agggaatgag gaagcctgct tttttgtcag 3301 gtgagcaaaa gaaggctatc gttgatctct tgttcaagac caacagaaag gtgaccgtga 3361 agcagctcaa agaggattac ttcaagaaaa tcgagtgctt cgattcagtt gagatttctg 3421 gtgttgagga taggttcaac gcatctctcg gaacctacca cgatctcctc aagatcatta 3481 aggataagga tttcttggat aacgaggaaa acgaggatat cttggaggat atcgttctta 3541 ccctcaccct ctttgaagat agagagatga ttgaagaaag gctcaagacc tacgctcatc 3601 tcttcgatga taaggtgatg aagcagttga agagaagaag atacactggt tggggaaggc 3661 tctcaagaaa gctcattaac ggaatcaggg ataagcagtc tggaaagaca atccttgatt 3721 tcctcaagtc tgatggattc gctaacagaa acttcatgca gctcatccac gatgattctc 3781 tcacctttaa agaggatatc cagaaggctc aggtttcagg acagggtgat agtctccatg 3841 agcatatcgc taacctcgct ggatctcctg caatcaagaa gggaatcctc cagactgtga 3901 aggttgtgga tgagttggtg aaggtgatgg gaaggcataa gcctgagaac atcgtgatcg 3961 aaatggctag agagaaccag accactcaga agggacagaa gaactctagg gaaaggatga 4021 agaggatcga ggaaggtatc aaagagcttg gatctcagat cctcaaagag caccctgttg 4081 agaacactca gctccagaat gagaagctct acctctacta cctccagaac ggaagggata 4141 tgtatgtgga tcaagagttg gatatcaaca ggctctctga ttacgatgtt gatgctatcg 4201 tgccacagtc attcttgaag gatgattcta tcgataacaa ggtgctcacc aggtctgata 4261 agaacagggg taagagtgat aacgtgccaa gtgaagaggt tgtgaagaaa atgaagaact 4321 attggaggca gctcctcaac gctaagctca tcactcagag aaagttcgat aacttgacta 4381 aggctgagag gggaggactc tctgaattgg ataaggcagg attcatcaag aggcagcttg 4441 tggaaaccag gcagatcact aagcacgttg cacagatcct cgattctagg atgaacacca 4501 agtacgatga gaacgataag ttgatcaggg aagtgaaggt tatcaccctc aagtcaaagc 4561 tcgtgtctga tttcagaaag gatttccaat tctacaaggt gagggaaatc aacaactacc 4621 accacgctca cgatgcttac cttaacgctg ttgttggaac cgctctcatc aagaagtatc 4681 ctaagctcga gtcagagttc gtgtacggtg attacaaggt gtacgatgtg aggaagatga 4741 tcgctaagtc tgagcaagag atcggaaagg ctaccgctaa gtatttcttc tactctaaca 4801 tcatgaattt cttcaagacc gagattaccc tcgctaacgg tgagatcaga aagaggccac 4861 tcatcgagac aaacggtgaa acaggtgaga tcgtgtggga taagggaagg gatttcgcta 4921 ccgttagaaa ggtgctctct atgccacagg tgaacatcgt taagaaaacc gaggtgcaga 4981 ccggtggatt ctctaaagag tctatcctcc ctaagaggaa ctctgataag ctcattgcta 5041 ggaagaagga ttgggaccct aagaaatacg gtggtttcga ttctcctacc gtggcttact 5101 ctgttctcgt tgtggctaag gttgagaagg gaaagagtaa gaagctcaag tctgttaagg 5161 aacttctcgg aatcactatc atggaaaggt catctttcga gaagaaccca atcgatttcc 5221 tcgaggctaa gggatacaaa gaggttaaga aggatctcat catcaagctc ccaaagtact 5281 cactcttcga actcgagaac ggtagaaaga ggatgctcgc ttctgctggt gagcttcaaa 5341 agggaaacga gcttgctctc ccatctaagt acgttaactt tctttacctc gcttctcact 5401 acgagaagtt gaagggatct ccagaagata acgagcagaa gcaacttttc gttgagcagc 5461 acaagcacta cttggatgag atcatcgagc agatctctga gttctctaaa agggtgatcc 5521 tcgctgatgc aaacctcgat aaggtgttgt ctgcttacaa caagcacaga gataagccta 5581 tcagggaaca ggcagagaac atcatccatc tcttcaccct taccaacctc ggtgctcctg 5641 ctgctttcaa gtacttcgat acaaccatcg ataggaagag atacacctct accaaagaag 5701 tgctcgatgc taccctcatc catcagtcta tcactggact ctacgagact aggatcgatc 5761 tctcacagct cggtggtgat tcgtcaaggg ctgatgaatt cccaaagaag aagcggaagg 5821 tggagctcag cggaggatct tccggaggat ctagcggctc cgagacacca ggaacatccg 5881 aaagcgctac accagaatct agcggaggct cttccggagg atctaggcct accctcaaca 5941 tcgaggatga gtatcgcctc cacgaaacct ccaaagaacc ggacgtgtcc ctcggcagca 6001 catggctcag cgacttccca caagcgtggg ccgaaaccgg cggcatgggc ctcgccgtcc 6061 gccaagcccc actcattatc ccgctgaagg cgacctccac accggtgtcc atcaagcagt 6121 acccgatgag ccaagaggcg aggctcggga ttaagccgca cattcagcgc ctcctcgatc 6181 aaggcattct cgtgccgtgc caatccccgt ggaatacacc actcctcccg gtcaaaaagc 6241 cgggcaccaa cgactatcgc ccggtccaag atctccgcga ggtcaacaag cgcgtggaag 6301 acatccaccc gaccgtcccg aacccgtata atctgctctc cgggctccca ccatcccacc 6361 agtggtatac agtgctggac ctcaaagacg ccttcttctg tctccgcctc cacccaacaa 6421 gccagccgct cttcgccttc gagtggcgcg acccggagat gggcatctcc ggccaactga 6481 catggacacg cctcccgcaa ggcttcaaga acagcccgac actcttcaac gaggcgctcc 6541 atagggacct cgcggatttt cgcatccagc atccggacct catcctcctc cagtatgtgg 6601 atgatctcct cctcgccgcg acctccgagc tggattgtca acaaggcaca cgcgcgctcc 6661 tccaaacact cgggaacctc ggctatcgcg cgtccgcgaa aaaggcccaa atctgccaga 6721 agcaagtgaa gtacctcggg tatctgctca aggaaggcca acgctggctc accgaagcgc 6781 gcaaagaaac agtgatgggg caaccgacac cgaaaacacc acgccagctg cgcgagtttc 6841 tcggcaaagc cggcttctgt cgcctcttca tcccgggctt tgccgagatg gccgcgccac 6901 tctacccact caccaagccg ggcacactgt ttaactgggg gccggatcag cagaaagcct 6961 accaagagat caaacaagcg ctcctcaccg ccccagcgct cgggctccca gatctcacaa 7021 agccgttcga gctgttcgtc gatgagaagc aaggctacgc gaagggcgtg ctcacacaga 7081 agctcggccc gtggaggagg ccagtggcct atctctccaa aaaactcgat ccagtggccg 7141 ccggctggcc accgtgtctg cgcatggtcg ccgcgattgc cgtgctcaca aaggatgccg 7201 gcaaactcac aatgggccag ccgctggtga tcctcgcgcc acatgccgtg gaagccctcg 7261 tcaaacagcc gccggatagg tggctctcca atgcgcgcat gacccattac caagcgctcc 7321 tcctcgacac cgatcgcgtc cagttcggcc cagtggtcgc cctcaatccg gcgacactgc 7381 tgccactccc agaggagggc ctccaacaca actgtctgga tattctcgcg gaagcgcatg 7441 gcacaaggcc agacctcaca gatcaaccgc tcccggatgc ggatcacaca tggtataccg 7501 acggctcctc tctgctccaa gagggccaaa ggaaagccgg cgccgcggtg accacagaaa 7561 cagaagtgat ctgggccaag gccctcccag ccggcacatc cgcgcaaagg gcggaactca 7621 tcgcgctcac acaagccctc aagatggccg agggcaagaa gctcaacgtc tacacagact 7681 cccgctatgc cttcgccacc gcccacattc acggcgaaat ctataggagg cgcggctggc 7741 tcacaagcga ggggaaggag atcaagaaca aggatgagat cctcgcgctg ctcaaggccc 7801 tctttctccc gaagcgcctc tccatcatcc actgtccggg ccaccaaaag gggcactccg 7861 cggaagcgag gggcaatagg atggccgatc aagccgcgcg caaagccgcg attaccgaaa 7921 ccccagacac atccaccctc ctcatcgaaa actcctcccc atccggcggc agcaagcgca 7981 cggcggacgg ctcggagttc gagccgaaga agaagcgcaa ggtgtgagct tcaggcctcc 8041 cagctttcgt ccgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca 8101 ttgcccacac accagaatcc tactaagttt gagtattatg gcattggaaa agctgttttc 8161 ttctatcatt tgttctgctt gtaatttact gtgttctttc agtttttgtt ttcggacatc 8221 aaaatgcaaa tggatggata agagttaata aatgatatgg tccttttgtt cattctcaaa 8281 ttattattat ctgttgtttt tactttaatg ggttgaattt aagtaagaaa ggaactaaca 8341 gtgtgatatt aaggtgcaat gttagacata taaaacagtc tttcacctct ctttggttat 8401 gtcttgaatt ggtttgtttc ttcacttatc tgtgtaatca agtttactat gagtctatga 8461 tcaagtaatt atgcaatcaa gttaagtaca gtataggctt cgcttgagac gaagctttga 8521 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt 8581 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg 8641 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt 8701 ctgacgttca gtgcagccgt catctgaaaa cgacatgtcg cacaagtcct aagttacgcg 8761 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa 8821 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg 8881 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga 8941 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga 9001 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac 9061 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca 9121 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc 9181 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat 9241 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg 9301 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg 9361 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact 9421 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga 9481 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc 9541 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg 9601 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acctctcaac cgtgcggctg 9661 catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc 9721 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg 9781 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac 9841 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa 9901 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc 9961 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg 10021 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag 10081 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact 10141 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg 10201 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt 10261 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg 10321 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg 10381 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc 10441 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa 10501 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt 10561 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga 10621 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc 10681 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt 10741 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat 10801 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc 10861 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc 10921 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg 10981 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg 11041 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt 11101 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg 11161 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac 11221 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca 11281 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat 11341 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc 11401 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa 11461 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg 11521 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc 11581 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat 11641 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat 11701 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca 11761 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga 11821 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag 11881 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac 11941 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt 12001 gccctagcag gggaaaaagg tcgaaaagga ctctttcctg tggatagcac gtacattggg 12061 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt 12121 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc 12181 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct 12241 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta 12301 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta 12361 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc 12421 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 12481 gcagctcccg gtgacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 12541 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag 12601 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg 12661 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc 12721 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 12781 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 12841 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 12901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 12961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 13021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 13081 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 13141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 13201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 13261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 13321 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 13381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 13441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 13501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 13561 gtcatgcatt ctaggtgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt 13621 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa 13681 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg 13741 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa 13801 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca 13861 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc 13921 gttattcatt cgtgattgcg cctgagcgag tcgaaatacg cgatcgctgt taaaaggaca 13981 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt 14041 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttccctg ggatcgcagt 14101 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat 14161 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc 14221 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc ggtagattgt 14281 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat 14341 gttggaattt aatcgcggcc ttgagcaaga cgtttcccgt tgaatatggc tcataacaga 14401 acttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata 14461 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag 14521 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga 14581 aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc 14641 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg 14701 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga 14761 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct 14821 ggctggtggc aggatatatt gtggtgtaaa cataacaagc tt //