LOCUS pDGB3_α1-StUBI-PPE2-tRbcS3a_4 14860 bp DNA circular SYN 06-NOV-2024 DEFINITION a1_StUBI-PPE2-tS3a_4. ACCESSION VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid. REFERENCE 1 (bases 1 to 14860) AUTHORS SnapGene License TITLE Direct Submission JOURNAL Exported Oct 21, 2024 from SnapGene 7.2.1 https://www.snapgene.com FEATURES Location/Qualifiers misc_feature 1..11 /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" promoter 12..1021 /label="PromStUbiQ10" 5'UTR 1022..1120 intron 1121..1614 NLS 1622..1675 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS BP" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP (NLS BP)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; BP (NLS BP translation)" /label="BP" CDS 1676..5776 /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AtCas9-H840A" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AtCas9-H840A" /Alternative_Match="99.98%; Adjusted-Interval-0:0/0; AtCas9-D10A,H840A" /Alternative_Match="99.95%; Adjusted-Interval-0:0/0; AtCas9" /Alternative_Match="99.93%; Adjusted-Interval-0:0/0; AtCas9-D10A" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SpCas9(H840A)" /Alternative_Match="99.93%; Adjusted-Interval-0:0/0; SpCas9" /Alternative_Match="99.93%; Adjusted-Interval-0:0/0; SpCas9(D10A,H840A)" /Alternative_Match="99.85%; Adjusted-Interval-0:0/0; SpCas9(D10A)" /label="AtCas9-H840A" NLS 5798..5818 /created_by="warddecaestecker" /modified_by="wacae" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS SV40" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /label="SV40" Linker 5825..5920 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="32-aa linker" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 32-aa linker" /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24; XTEN (32-aa linker)" /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24; XTEN" /label="32-aa linker" CDS 5927..7957 /note="Reverse transcriptase from Moloney murine leukemia virus. To be used for prime editing" /modified_by="mamee" /label="MoMLV-RT" NLS 7970..8020 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS BP*" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (NLS BP*)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; BP* (NLS BP* translation)" /Alternative_Match="100.00%; Adjusted-Interval-0:30/0; SV40 (NLS SV40)" /label="BP*" terminator 8028..8496 /created_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Pea3AT" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Pea3AT" /label="Pea3AT" misc_feature complement(8501..8511) /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" T-DNA_border 8517..8541 /note="right border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="RB T-DNA repeat" regulatory 9842..10471 /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" regulatory 10900..11972 /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" rep_origin 12038..12232 /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label="pVS1 oriV" regulatory 12606..12866 /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" rep_origin complement(12902..13490) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pColE1 ori" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pColE1 ori" /Alternative_Match="99.83%; Adjusted-Interval-0:0/0; pUC ori" /label="pColE1 ori" misc_feature complement(13578..14393) /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="98.53%" /Primary_Match="98.53%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" T-DNA_border 14826..14850 /note="left border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="LB T-DNA repeat" ORIGIN 1 cgtctcagga gccaagacaa tttcagctta aaaagtttta ttaatattta cattagtttt 61 gttgatgagg atgacaagat tttggtcatc aattacatat acccaaattg aatacttagt 121 aagcaactta atgtttttca taatgataat gacagacaca aaaaaaaccc atttattatt 181 cacattgatt gatttttata tgcactatag taataataat aatatttctt ataaagcaag 241 aggtcaattt tttattttat tataccaacg acactaaatt atatttgata atgtaaaaca 301 attcaatttt acttaaatat catgaaataa actattttta taaccaaatt actaaattta 361 tccaataaaa aaaagtcatt aagaagacat aaaataaatt tgagtaaaaa gagtgaagtc 421 gactgacttt tttttttatc ataagaaaat aaattattaa ctttaaccta ataaaacact 481 aatataattt catggaatct aatacttacc tcttagatat aagaaaaagc gtttctaata 541 gaccctcaat ttacattaaa tattttcaat caagtttaaa taacaaatat caatatgagg 601 tcaataacag tatcaaaata atatgaaaaa agagcaatac ataatataag aaagaagatt 661 taagtgcact tatcaaggta gtattatatc ctaatttgct aatatttaaa ctcttatatt 721 taaggtcatg ttcacgataa acttgaaatg cgctttatta gagcatatat taaaataaaa 781 aaaataccta aaataaaata aagttatttt tagtatatat tttttacatg acctacattt 841 ttctagtttt ttctaaagga gcgtgtaagt gtcaacctca ttctcctaat tttccccacc 901 acataaaaat taaaaaggaa aggtagcttt tgcgtgttgt tttggtacac tacacctcat 961 tattacacgt gtcctcatat agttggttaa cccgtgaggc ggtttcctct agagtcggcc 1021 atgccatcta taaaatgaag ctttctgcac ctcaattttt catcttctat ctgatttcta 1081 ttataatttc tattaattgc cttcaaattt ctctttcaag gttagaaatc ttctctattt 1141 tttggttttt gtctgtttag attctcgaat tagctaatca ggtgctgtta aagccctaaa 1201 atttgagttt tttttccgtc gaattgatgc taaaggctta aaattagagt tttttcgtcg 1261 gtttgactct gaaggcctaa aatttggggt tttccgggtg atttgatgat aaagccctag 1321 aatttgagtt tttttatttg tcggtttgat gaaaaaggcc ttaaatttaa tttttttccc 1381 ggttgatttg atgaaaaagc cctagaattt gtgttttttc gtcggtttga ttctaaaggc 1441 ctaaaatttg agtttttccg gttgttttga tgaaaaagcc ctaaaatttg agttttttcc 1501 ccgtgtttta gattgtttgg ttttaattct tgaatcagat aatcagggag tgtgaaaagc 1561 cctaaaattt gagttttttt cgttgttctg attgttgttt ttatgaattt gcagaatgat 1621 gaagagaact gctgatggat ctgaatttga atctcctaag aagaagagaa aggttgataa 1681 gaagtactct atcggactcg atatcggaac taactctgtg ggatgggctg tgatcaccga 1741 tgagtacaag gtgccatcta agaagttcaa ggttctcgga aacaccgata ggcactctat 1801 caagaaaaac cttatcggtg ctctcctctt cgattctggt gaaactgctg aggctaccag 1861 actcaagaga accgctagaa gaaggtacac cagaagaaag aacaggatct gctacctcca 1921 agagatcttc tctaacgaga tggctaaagt ggatgattca ttcttccaca ggctcgaaga 1981 gtcattcctc gtggaagaag ataagaagca cgagaggcac cctatcttcg gaaacatcgt 2041 tgatgaggtg gcataccacg agaagtaccc tactatctac cacctcagaa agaagctcgt 2101 tgattctact gataaggctg atctcaggct catctacctc gctctcgctc acatgatcaa 2161 gttcagagga cacttcctca tcgagggtga tctcaaccct gataactctg atgtggataa 2221 gttgttcatc cagctcgtgc agacctacaa ccagcttttc gaagagaacc ctatcaacgc 2281 ttcaggtgtg gatgctaagg ctatcctctc tgctaggctc tctaagtcaa gaaggcttga 2341 gaacctcatt gctcagctcc ctggtgagaa gaagaacgga cttttcggaa acttgatcgc 2401 tctctctctc ggactcaccc ctaacttcaa gtctaacttc gatctcgctg aggatgcaaa 2461 gctccagctc tcaaaggata cctacgatga tgatctcgat aacctcctcg ctcagatcgg 2521 agatcagtac gctgatttgt tcctcgctgc taagaacctc tctgatgcta tcctcctcag 2581 tgatatcctc agagtgaaca ccgagatcac caaggctcca ctctcagctt ctatgatcaa 2641 gagatacgat gagcaccacc aggatctcac acttctcaag gctcttgtta gacagcagct 2701 cccagagaag tacaaagaga ttttcttcga tcagtctaag aacggatacg ctggttacat 2761 cgatggtggt gcatctcaag aagagttcta caagttcatc aagcctatcc tcgagaagat 2821 ggatggaacc gaggaactcc tcgtgaagct caatagagag gatcttctca gaaagcagag 2881 gaccttcgat aacggatcta tccctcatca gatccacctc ggagagttgc acgctatcct 2941 tagaaggcaa gaggatttct acccattcct caaggataac agggaaaaga ttgagaagat 3001 tctcaccttc agaatccctt actacgtggg acctctcgct agaggaaact caagattcgc 3061 ttggatgacc agaaagtctg aggaaaccat caccccttgg aacttcgaag aggtggtgga 3121 taagggtgct agtgctcagt ctttcatcga gaggatgacc aacttcgata agaaccttcc 3181 aaacgagaag gtgctcccta agcactcttt gctctacgag tacttcaccg tgtacaacga 3241 gttgaccaag gttaagtacg tgaccgaggg aatgaggaag cctgcttttt tgtcaggtga 3301 gcaaaagaag gctatcgttg atctcttgtt caagaccaac agaaaggtga ccgtgaagca 3361 gctcaaagag gattacttca agaaaatcga gtgcttcgat tcagttgaga tttctggtgt 3421 tgaggatagg ttcaacgcat ctctcggaac ctaccacgat ctcctcaaga tcattaagga 3481 taaggatttc ttggataacg aggaaaacga ggatatcttg gaggatatcg ttcttaccct 3541 caccctcttt gaagatagag agatgattga agaaaggctc aagacctacg ctcatctctt 3601 cgatgataag gtgatgaagc agttgaagag aagaagatac actggttggg gaaggctctc 3661 aagaaagctc attaacggaa tcagggataa gcagtctgga aagacaatcc ttgatttcct 3721 caagtctgat ggattcgcta acagaaactt catgcagctc atccacgatg attctctcac 3781 ctttaaagag gatatccaga aggctcaggt ttcaggacag ggtgatagtc tccatgagca 3841 tatcgctaac ctcgctggat ctcctgcaat caagaaggga atcctccaga ctgtgaaggt 3901 tgtggatgag ttggtgaagg tgatgggaag gcataagcct gagaacatcg tgatcgaaat 3961 ggctagagag aaccagacca ctcagaaggg acagaagaac tctagggaaa ggatgaagag 4021 gatcgaggaa ggtatcaaag agcttggatc tcagatcctc aaagagcacc ctgttgagaa 4081 cactcagctc cagaatgaga agctctacct ctactacctc cagaacggaa gggatatgta 4141 tgtggatcaa gagttggata tcaacaggct ctctgattac gatgttgatg ctatcgtgcc 4201 acagtcattc ttgaaggatg attctatcga taacaaggtg ctcaccaggt ctgataagaa 4261 caggggtaag agtgataacg tgccaagtga agaggttgtg aagaaaatga agaactattg 4321 gaggcagctc ctcaacgcta agctcatcac tcagagaaag ttcgataact tgactaaggc 4381 tgagagggga ggactctctg aattggataa ggcaggattc atcaagaggc agcttgtgga 4441 aaccaggcag atcactaagc acgttgcaca gatcctcgat tctaggatga acaccaagta 4501 cgatgagaac gataagttga tcagggaagt gaaggttatc accctcaagt caaagctcgt 4561 gtctgatttc agaaaggatt tccaattcta caaggtgagg gaaatcaaca actaccacca 4621 cgctcacgat gcttacctta acgctgttgt tggaaccgct ctcatcaaga agtatcctaa 4681 gctcgagtca gagttcgtgt acggtgatta caaggtgtac gatgtgagga agatgatcgc 4741 taagtctgag caagagatcg gaaaggctac cgctaagtat ttcttctact ctaacatcat 4801 gaatttcttc aagaccgaga ttaccctcgc taacggtgag atcagaaaga ggccactcat 4861 cgagacaaac ggtgaaacag gtgagatcgt gtgggataag ggaagggatt tcgctaccgt 4921 tagaaaggtg ctctctatgc cacaggtgaa catcgttaag aaaaccgagg tgcagaccgg 4981 tggattctct aaagagtcta tcctccctaa gaggaactct gataagctca ttgctaggaa 5041 gaaggattgg gaccctaaga aatacggtgg tttcgattct cctaccgtgg cttactctgt 5101 tctcgttgtg gctaaggttg agaagggaaa gagtaagaag ctcaagtctg ttaaggaact 5161 tctcggaatc actatcatgg aaaggtcatc tttcgagaag aacccaatcg atttcctcga 5221 ggctaaggga tacaaagagg ttaagaagga tctcatcatc aagctcccaa agtactcact 5281 cttcgaactc gagaacggta gaaagaggat gctcgcttct gctggtgagc ttcaaaaggg 5341 aaacgagctt gctctcccat ctaagtacgt taactttctt tacctcgctt ctcactacga 5401 gaagttgaag ggatctccag aagataacga gcagaagcaa cttttcgttg agcagcacaa 5461 gcactacttg gatgagatca tcgagcagat ctctgagttc tctaaaaggg tgatcctcgc 5521 tgatgcaaac ctcgataagg tgttgtctgc ttacaacaag cacagagata agcctatcag 5581 ggaacaggca gagaacatca tccatctctt cacccttacc aacctcggtg ctcctgctgc 5641 tttcaagtac ttcgatacaa ccatcgatag gaagagatac acctctacca aagaagtgct 5701 cgatgctacc ctcatccatc agtctatcac tggactctac gagactagga tcgatctctc 5761 acagctcggt ggtgattcgt caagggctga tgaattccca aagaagaagc ggaaggtgga 5821 gctcagcgga ggatcttccg gaggatctag cggctccgag acaccaggaa catccgaaag 5881 cgctacacca gaatctagcg gaggctcttc cggaggatct aggcctaccc tcaacatcga 5941 ggatgagtat cgcctccacg aaacctccaa agaaccggac gtgtccctcg gcagcacatg 6001 gctcagcgac ttcccacaag cgtgggccga aaccggcggc atgggcctcg ccgtccgcca 6061 agccccactc attatcccgc tgaaggcgac ctccacaccg gtgtccatca agcagtaccc 6121 gatgagccaa gaggcgaggc tcgggattaa gccgcacatt cagcgcctcc tcgatcaagg 6181 cattctcgtg ccgtgccaat ccccgtggaa tacaccactc ctcccggtca aaaagccggg 6241 caccaacgac tatcgcccgg tccaagatct ccgcgaggtc aacaagcgcg tggaagacat 6301 ccacccgacc gtcccgaacc cgtataatct gctctccggg ctcccaccat cccaccagtg 6361 gtatacagtg ctggacctca aagacgcctt cttctgtctc cgcctccacc caacaagcca 6421 gccgctcttc gccttcgagt ggcgcgaccc ggagatgggc atctccggcc aactgacatg 6481 gacacgcctc ccgcaaggct tcaagaacag cccgacactc ttcaacgagg cgctccatag 6541 ggacctcgcg gattttcgca tccagcatcc ggacctcatc ctcctccagt atgtggatga 6601 tctcctcctc gccgcgacct ccgagctgga ttgtcaacaa ggcacacgcg cgctcctcca 6661 aacactcggg aacctcggct atcgcgcgtc cgcgaaaaag gcccaaatct gccagaagca 6721 agtgaagtac ctcgggtatc tgctcaagga aggccaacgc tggctcaccg aagcgcgcaa 6781 agaaacagtg atggggcaac cgacaccgaa aacaccacgc cagctgcgcg agtttctcgg 6841 caaagccggc ttctgtcgcc tcttcatccc gggctttgcc gagatggccg cgccactcta 6901 cccactcacc aagccgggca cactgtttaa ctgggggccg gatcagcaga aagcctacca 6961 agagatcaaa caagcgctcc tcaccgcccc agcgctcggg ctcccagatc tcacaaagcc 7021 gttcgagctg ttcgtcgatg agaagcaagg ctacgcgaag ggcgtgctca cacagaagct 7081 cggcccgtgg aggaggccag tggcctatct ctccaaaaaa ctcgatccag tggccgccgg 7141 ctggccaccg tgtctgcgca tggtcgccgc gattgccgtg ctcacaaagg atgccggcaa 7201 actcacaatg ggccagccgc tggtgatcct cgcgccacat gccgtggaag ccctcgtcaa 7261 acagccgccg gataggtggc tctccaatgc gcgcatgacc cattaccaag cgctcctcct 7321 cgacaccgat cgcgtccagt tcggcccagt ggtcgccctc aatccggcga cactgctgcc 7381 actcccagag gagggcctcc aacacaactg tctggatatt ctcgcggaag cgcatggcac 7441 aaggccagac ctcacagatc aaccgctccc ggatgcggat cacacatggt ataccgacgg 7501 ctcctctctg ctccaagagg gccaaaggaa agccggcgcc gcggtgacca cagaaacaga 7561 agtgatctgg gccaaggccc tcccagccgg cacatccgcg caaagggcgg aactcatcgc 7621 gctcacacaa gccctcaaga tggccgaggg caagaagctc aacgtctaca cagactcccg 7681 ctatgccttc gccaccgccc acattcacgg cgaaatctat aggaggcgcg gctggctcac 7741 aagcgagggg aaggagatca agaacaagga tgagatcctc gcgctgctca aggccctctt 7801 tctcccgaag cgcctctcca tcatccactg tccgggccac caaaaggggc actccgcgga 7861 agcgaggggc aataggatgg ccgatcaagc cgcgcgcaaa gccgcgatta ccgaaacccc 7921 agacacatcc accctcctca tcgaaaactc ctccccatcc ggcggcagca agcgcacggc 7981 ggacggctcg gagttcgagc cgaagaagaa gcgcaaggtg tgagcttcag gcctcccagc 8041 tttcgtccgt atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc 8101 ccacacacca gaatcctact aagtttgagt attatggcat tggaaaagct gttttcttct 8161 atcatttgtt ctgcttgtaa tttactgtgt tctttcagtt tttgttttcg gacatcaaaa 8221 tgcaaatgga tggataagag ttaataaatg atatggtcct tttgttcatt ctcaaattat 8281 tattatctgt tgtttttact ttaatgggtt gaatttaagt aagaaaggaa ctaacagtgt 8341 gatattaagg tgcaatgtta gacatataaa acagtctttc acctctcttt ggttatgtct 8401 tgaattggtt tgtttcttca cttatctgtg taatcaagtt tactatgagt ctatgatcaa 8461 gtaattatgc aatcaagtta agtacagtat aggcttcgct gtcatgagac gaattctgac 8521 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt 8581 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt 8641 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc 8701 tgacgttcag tgcagccgtc atctgaaaac gacatgtcgc acaagtccta agttacgcga 8761 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag 8821 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc 8881 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa 8941 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac 9001 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc 9061 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag 9121 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc 9181 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc 9241 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc 9301 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc 9361 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt 9421 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac 9481 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca 9541 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg 9601 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cctctcaacc gtgcggctgc 9661 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg 9721 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc 9781 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg 9841 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac 9901 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc 9961 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg 10021 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt 10081 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt 10141 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga 10201 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc 10261 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga 10321 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc 10381 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct 10441 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa 10501 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg 10561 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat 10621 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct 10681 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt 10741 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg 10801 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc 10861 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg 10921 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc 10981 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg 11041 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta 11101 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg 11161 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc 11221 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag 11281 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc 11341 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc 11401 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag 11461 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga 11521 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct 11581 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt 11641 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt 11701 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag 11761 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag 11821 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt 11881 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc 11941 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg 12001 ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt ggatagcacg tacattggga 12061 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg 12121 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct 12181 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg 12241 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac 12301 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac 12361 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc 12421 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 12481 cagctcccgg tgacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 12541 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc 12601 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 12661 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct 12721 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 12781 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 12841 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 12901 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 12961 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 13021 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 13081 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 13141 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 13201 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 13261 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 13321 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 13381 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 13441 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 13501 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 13561 tcatgcattc taggtgatta gaaaaactca tcgagcatca aatgaaactg caatttattc 13621 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac 13681 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt 13741 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa 13801 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag 13861 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg 13921 ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc gatcgctgtt aaaaggacaa 13981 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt 14041 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttccctgg gatcgcagtg 14101 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata 14161 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct 14221 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg gtagattgtc 14281 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg 14341 ttggaattta atcgcggcct tgagcaagac gtttcccgtt gaatatggct cataacagaa 14401 cttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa 14461 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc 14521 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa 14581 accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg 14641 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt 14701 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat 14761 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg 14821 gctggtggca ggatatattg tggtgtaaac ataacgaatt //