LOCUS pDGB3_α1-pZmUbi-zCas9iRY-tRbcSE9 13419 bp DNA circular SYN 06-NOV-2024 DEFINITION a1_pZmUbi-zCas9iRY-tRbcSE9. ACCESSION VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid. REFERENCE 1 (bases 1 to 13419) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported Feb 6, 2023 from SnapGene 6.2.1 https://www.snapgene.com FEATURES Location/Qualifiers misc_feature 1..11 /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" promoter 12..1517 /modified_by="mamee" /label="pZm Ubc" NLS 1526..1546 /created_by="warddecaestecker" /modified_by="wacae" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS SV40" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /label="SV40" CDS join(1571..1843,1974..2310,2402..2635,2744..2992, 3094..5553,5713..6023,6133..6369) /note="TwistBioscience synthesis, adapted from Grutzner et al. 2021 and Walton et al., 2020" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="zCas9i-RY" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0, Adjusted-Interval-1:0/0, Adjusted-Interval-2:0/0, Adjusted-Interval-3:0/0, Adjusted-Interval-4:0/0, Adjusted-Interval-5:0/0, Adjusted-Interval-6:0/0; zCas9i-RY" /label="zCas9i-RY" NLS 6370..6417 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on protein similarity" /Transferred_From="NLS Nucleoplasmin" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Nucleoplasmin (NLS Nucleoplasmin)" /label="Nucleoplasmin" terminator 6421..7055 /note="note=""Fragment extracted from fragmentTRbcSE9.txt.xdna [639 nt] : (#BsmBI[5] / #BsmBI[660])" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="tRbcSE9" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tRbcSE9" /label="tRbcSE9" misc_feature complement(7060..7070) /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" T-DNA_border 7076..7100 /note="right border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="RB T-DNA repeat" regulatory 8401..9030 /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" regulatory 9459..10531 /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" rep_origin 10597..10791 /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label="pVS1 oriV" regulatory 11165..11425 /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" rep_origin complement(11461..12049) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pColE1 ori" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pColE1 ori" /Alternative_Match="99.83%; Adjusted-Interval-0:0/0; pUC ori" /label="pColE1 ori" misc_feature complement(12137..12952) /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="98.53%" /Primary_Match="98.53%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" T-DNA_border 13385..13409 /note="left border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="LB T-DNA repeat" ORIGIN 1 cgtctcagga gctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca 61 ttgcatgtct aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg 121 cagtttatct atctttatac atatatttaa actttactct acgaataata taatctatag 181 tactacaata atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa 241 aggacaattg agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg 301 ttctcctttt tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac 361 atccatttag ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt 421 tattctattt tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa 481 taatttagat ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa 541 gaaattaaaa aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta 601 aacgccgtcg atcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg 661 gccaagcgaa gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt 721 ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca 781 gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg 841 gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc 901 ccctccacac cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga 961 tctcccccaa atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc 1021 ccccccctct ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc 1081 tacttctgtt catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc 1141 gtacacggat gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc 1201 tttggggaat cctgggatgg ctctagccgt tccgcagacg ggatcgatct aggataggta 1261 tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca 1321 tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt 1381 gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct 1441 gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt 1501 ttggtgttac ttctgcagaa tggcgcctaa gaagaagcgc aaggttggga tccacggggt 1561 tccggcggct gacaagaagt acagcattgg acttgatatt ggtacgaact cagttgggtg 1621 ggccgttatc accgatgaat acaaggtacc ttcgaagaaa tttaaagtgc tgggcaacac 1681 agataggcac agcattaaga agaacttgat cggagctctg ctctttgact ctggagaaac 1741 cgcggagagg acaaggctta aacgtactgc gaggagaagg tacactcgca ggaagaacag 1801 aatctgttat ctccaagaga tctttagcaa cgagatggcg aaggtaagga tttttatgat 1861 atagtatgct tatgtatttt gtactgaaag catatcctgc ttcattggga tattactgaa 1921 agcatttaac tacatgtaaa ctcacttgat gatcaataaa cttgattttg caggttgacg 1981 actcgttctt ccatcgcctc gaggaatcct tcctggtaga ggaagataag aaacacgagc 2041 gtcaccccat ctttgggaat attgttgacg aagtagccta tcatgaaaag tatccgacta 2101 tataccacct tcgcaagaag ctggtggact caaccgataa ggcagacctt cggctcatat 2161 acctggctct cgcgcacatg ataaagtttc gtggccattt cttgatcgaa ggggacctca 2221 acccggataa ctccgatgtg gataaactgt tcattcagct cgtccaaacc tacaatcagc 2281 tgttcgagga gaaccccatc aatgcatcag gtaacattcc ttagttacct ttcttttctt 2341 tttccatcat aagtttatag attgtacatg ctttgagatt tttctttgca aacaatctca 2401 ggtgtcgacg ccaaggcaat actgtctgcc agactttcga agtccagacg gcttgagaat 2461 ctgatcgctc aattgccagg cgagaagaag aacggcttgt tcgggaatct gattgcactg 2521 tctctgggcc tcacccctaa cttcaaaagc aactttgacc tcgccgagga cgcgaagctg 2581 cagctgtcaa aggatacata cgatgatgat ctggacaatc tgctcgccca aataggtgct 2641 cttgaaattg gaactcttct tttgttgtct aaacctatca atttctttgc ggaaatttat 2701 ttgaagctgt agagttaaaa ttgagtcttt taaacttttg taggtgatca gtatgccgac 2761 ctgttcttgg ctgccaagaa tctgtcagac gctatcttgc tcagtgacat tctgcgggtc 2821 aacacggaga taaccaaagc gccacttagc gcctccatga tcaagaggta cgacgagcat 2881 caccaggatc tgacccttct gaaggctttg gttcgccagc aactccccga gaagtacaag 2941 gagattttct ttgaccaatc gaagaatggc tacgcagggt acattgatgg aggtaagttg 3001 ttacttatga ttgttttcct ctctgctaca tgtattttgt tgttcatttc tgtaagatat 3061 aagaattgag ttttcctctg atgatattat taggtgcaag tcaggaggaa ttctacaaat 3121 tcatcaagcc tattctggaa aagatggacg gtacagagga gctgctcgtt aaattgaacc 3181 gcgaagattt gcttcggaag cagcgtacct tcgacaatgg cagcataccg caccagatcc 3241 acctcggtga gctgcatgct atcttgagga ggcaagagga cttctatccg ttcctgaaag 3301 acaacagaga gaagattgaa aagatcctca cgttccgcat tccctactat gtaggtccac 3361 tcgcacgcgg gaactcgcgg tttgcgtgga tgacacgcaa atccgaggag actatcacgc 3421 cttggaactt cgaagaggtc gtggacaagg gtgcgagtgc acagtccttc atcgaaagga 3481 tgaccaactt cgataagaat ctcccaaatg agaaagtcct gcccaagcat agtctcctgt 3541 acgaatactt cacggtctac aacgagctga cgaaggtgaa atatgtgacg gaggggatgc 3601 gcaaaccggc cttcctgtca ggtgagcaga agaaggccat tgtcgatctc ttgttcaaaa 3661 ccaatcggaa ggtcactgtg aaacagctta aagaggacta ctttaagaag atcgaatgct 3721 ttgattctgt ggaaatcagc ggcgttgagg ataggttcaa tgcctctctt ggcacatacc 3781 atgacctgtt gaaaatcatc aaggacaagg acttccttga caacgaggag aacgaggaca 3841 tcctcgagga catcgtgctg actctcacgc tgtttgagga cagagaaatg atcgaggagc 3901 gccttaagac ttatgcgcat ctgttcgatg acaaggtcat gaagcagttg aagaggagga 3961 gatatacagg ttggggaagg ctctccagga agctcatcaa cggcatccgc gacaagcaat 4021 ccggcaagac tatactggac tttctcaaat ccgacggttt tgcgaatcgg aacttcatgc 4081 agcttattca cgatgactca ctgaccttca aagaagatat ccagaaggcc caagtgtcag 4141 gtcagggcga tagccttcac gaacacatag ccaacctggc tggatcgcca gctataaaga 4201 agggcatact gcagacagtg aaggttgtgg atgagctggt gaaggtcatg ggccgccata 4261 agccggagaa catcgtcatc gagatggcga gggaaaacca gacgactcag aaagggcaga 4321 agaactcacg ggagcgcatg aagcggatag aggaaggcat caaggagctt gggagtcaga 4381 ttctgaaaga gcacccagtc gaaaatactc aactccagaa cgagaagctg tacctctatt 4441 acctccagaa tgggagagat atgtacgtcg accaagagct cgacattaac agactctccg 4501 actatgatgt ggatcacatt gtccctcaat ctttcctgaa ggacgatagt attgacaaca 4561 aggtccttac gcgctcagac aagaaccgcg gaaaatccga caatgtaccc agcgaggagg 4621 ttgtgaagaa gatgaagaac tattggaggc agcttttgaa tgctaagctc ataacccaac 4681 ggaaattcga caatctcacg aaggcagaaa ggggcggact gtctgagctc gacaaagccg 4741 gcttcatcaa gcgccagttg gttgaaactc gtcagattac gaaacatgtg gcccagatac 4801 tcgattcgcg tatgaatacg aagtatgatg agaatgacaa acttatcagg gaggtgaagg 4861 tgatcaccct caagagcaaa ctggttagtg acttccggaa ggacttccag ttttacaagg 4921 ttcgcgagat caacaactac catcatgccc atgacgccta cctgaacgcc gttgttggca 4981 ctgctctcat caagaagtat ccgaaactgg agtctgagtt tgtgtacggg gattacaagg 5041 tgtacgacgt taggaagatg atcgcgaagt cagaacaaga gatcggcaag gctaccgcga 5101 aatacttctt ttactcgaat atcatgaact tcttcaagac agagatcact ctggcgaatg 5161 gtgaaatccg gaagaggcct ctgatcgaga caaatggcga aacaggtgag attgtctggg 5221 ataagggcag ggattttgcg actgtgcgta aggttctcag catgccccaa gtcaacatag 5281 tcaagaaaac ggaggttcaa accggtggtt tctccaagga gtccattcgc cctaagcgca 5341 actccgacaa actgattgcg aggaagaagg attgggatcc gaagaaatac ggaggctttc 5401 tgtggcctac cgtggcatac agcgtactgg tagtggccaa ggtggagaag ggcaagagca 5461 agaaactgaa aagcgtcaag gaactgcttg gaattaccat aatggaaagg tcctcgttcg 5521 agaagaatcc gatcgacttc ctcgaggcta aaggtaaaat attggatgcc agacgatatt 5581 ctttcttttg atttgtaact ttttcctgtc aaggtcgata aattttattt tttttggtaa 5641 aaggtcgata attttttttt ggagccatta tgtaattttc ctaattaact gaaccaaaat 5701 tatacaaacc aggttacaaa gaggtgaaga aagacctcat tatcaaactg cccaagtatt 5761 cgcttttcga attggaaaat ggcagaaaac gcatgctggc atctgccaag caactgcaga 5821 agggcaacga gctggcattg cccagtaagt acgtcaactt cctgtacttg gcctcacact 5881 atgagaagct gaaggggtca ccagaggaca acgagcagaa gcagttgttt gtcgagcagc 5941 acaagcacta tcttgatgag atcatagagc agatcagcga attttccaag cgggtcattc 6001 ttgcagacgc taacctcgat aaggtaagga cttctcatga atattagtgg cagattagtg 6061 ttgttaaagt ctttggttag ataatcgatg cctcctaatt gtccatgttt tactggtttt 6121 ctacaattaa aggtgctttc cgcgtacaac aagcacagag ataagccgat aagggaacaa 6181 gcggaaaaca tcatccacct gttcacactg accaggctgg gagccccacg cgcctttaag 6241 tacttcgata ccactatcga cccaaagcag tacaggtcaa ccaaggaagt gttggacgct 6301 acccttattc accaatctat tacagggctc tatgagacaa ggatagatct gtcgcagttg 6361 ggtggtgaca agaggccggc ggcaaccaag aaggcgggcc aggccaagaa gaagaagtaa 6421 gcttgctttc gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt 6481 cattgcgcac acaccagaat cctactgagt ttgagtatta tggcattggg aaaactgttt 6541 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg 6601 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt 6661 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa 6721 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg 6781 aagttaatat gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac 6841 aaatatattt tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg 6901 tgttttagac atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta 6961 atcattgctt tataattata gttatactca tggatttgta gttgagtatg aaaatatttt 7021 ttaatgcatt ttatgacttg ccaattgatt gacaacgctg tcatgagacg aattctgaca 7081 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta 7141 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt 7201 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct 7261 gacgttcagt gcagccgtca tctgaaaacg acatgtcgca caagtcctaa gttacgcgac 7321 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt 7381 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc 7441 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac 7501 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc 7561 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca 7621 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg 7681 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg 7741 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg 7801 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc 7861 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca 7921 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg 7981 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg 8041 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat 8101 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga 8161 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg tgcggctgca 8221 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc 8281 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg 8341 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc 8401 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc 8461 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc 8521 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc 8581 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg 8641 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc 8701 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag 8761 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg 8821 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag 8881 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc 8941 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg 9001 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa 9061 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg 9121 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc 9181 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg 9241 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta 9301 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc 9361 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc 9421 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca aaccatccgg 9481 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca 9541 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc 9601 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag 9661 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg 9721 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg 9781 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg 9841 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct 9901 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg 9961 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga 10021 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa 10081 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta 10141 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg 10201 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta 10261 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg 10321 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga 10381 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta 10441 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct 10501 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc 10561 cctagcaggg gaaaaaggtc gaaaaggact ctttcctgtg gatagcacgt acattgggaa 10621 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg 10681 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta 10741 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg 10801 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg 10861 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg 10921 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc 10981 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 11041 agctcccggt gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 11101 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg 11161 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 11221 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc 11281 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 11341 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 11401 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 11461 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 11521 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 11581 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 11641 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 11701 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 11761 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 11821 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 11881 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 11941 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 12001 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 12061 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 12121 catgcattct aggtgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca 12181 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact 12241 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc 12301 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat 12361 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga 12421 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt 12481 tattcattcg tgattgcgcc tgagcgagtc gaaatacgcg atcgctgtta aaaggacaat 12541 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt 12601 cacctgaatc aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg 12661 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa 12721 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt 12781 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcgg tagattgtcg 12841 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt 12901 tggaatttaa tcgcggcctt gagcaagacg tttcccgttg aatatggctc ataacagaac 12961 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac 13021 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct 13081 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa 13141 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc 13201 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta 13261 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg 13321 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg 13381 ctggtggcag gatatattgt ggtgtaaaca taacgaatt //