LOCUS pDGB3_α1-NeoR-pZmUbi-pcoCas9-tRbcS3c 10412 bp DNA circular SYN 06-NOV-2024 DEFINITION a1_NeoR-pZmUbi-pcoCas9-tRbcS3a. ACCESSION VERSION . KEYWORDS a1 Neo_ccd pZmUBI pcoCas9. SOURCE synthetic DNA construct ORGANISM recombinant plasmid. REFERENCE 1 (bases 1 to 10412) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported Feb 6, 2023 from SnapGene 6.2.1 https://www.snapgene.com FEATURES Location/Qualifiers misc_feature 1..11 /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" promoter 12..2003 /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pZmUBIL.2" /Transferred_Similarity="99.75%" /Primary_Match="99.75%; Adjusted-Interval-0:0/0; ZmUBIL.2 (pZmUBIL.2)" /Alternative_Match="99.75%; Adjusted-Interval-0:0/-1; ZmUBIL (pZmUBIL)" /label="ZmUBIL.2" intron 990..2003 /created_by="lapau" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pZmUBIL.2" /Transferred_Similarity="99.61%" /Primary_Match="99.61%; Adjusted-Interval-0:0/0; ZmUBI intron (pZmUBIL.2)" /label="ZmUBI intron" CDS 2010..6125 /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AtCas9" /Transferred_Similarity="100.00%" /Adjusted_Transferred_Similarity="99.13%" /Primary_Match="100.00%; Adjusted-Interval-0:0/-36 (adjusted to 99.13%; Adjusted-Interval-0:0/0); AtCas9" /Alternative_Match="99.98%; Adjusted-Interval-0:0/-36 (adjusted to 99.11%; Adjusted-Interval-0:0/0); AtCas9-D10A" /Alternative_Match="99.95%; Adjusted-Interval-0:0/-36 (adjusted to 99.08%; Adjusted-Interval-0:0/0); AtCas9-H840A" /Alternative_Match="99.93%; Adjusted-Interval-0:0/-36 (adjusted to 99.06%; Adjusted-Interval-0:0/0); AtCas9-D10A,H840A" /Alternative_Match="100.00%; Adjusted-Interval-0:3/-36 (adjusted to 99.06%; Adjusted-Interval-0:0/0); SpCas9" /Alternative_Match="99.93%; Adjusted-Interval-0:3/-36 (adjusted to 98.99%; Adjusted-Interval-0:0/0); SpCas9(D10A)" /Alternative_Match="99.93%; Adjusted-Interval-0:3/-36 (adjusted to 98.99%; Adjusted-Interval-0:0/0); SpCas9(H840A)" /Alternative_Match="99.85%; Adjusted-Interval-0:3/-36 (adjusted to 98.91%; Adjusted-Interval-0:0/0); SpCas9(D10A,H840A)" /label="AtCas9" NLS 6126..6146 /created_by="wacae" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="NLS SV40" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /label="SV40" terminator 6153..6431 /note="Terminator of Tomato rbcS3C gene for ribulose 1,5-biphosphate carboxylase/oxygenase small subunit" /modified_by="mamee" /label="tRbcS3c" misc_feature complement(6436..6446) /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" promoter 6452..6913 /note="strong constitutive promoter from cauliflower mosaic virus" /label="CaMV 35S promoter" CDS 6914..7921 /codon_start=1 /translation="MTHNPTILRKTLPLYKEVHFIWRGQGTHQKATAYLNTSLETEKI RPPSMENIEKLLMQEKILMLELDLVRAKISLDGLHAGSPAAWVERLFGYDWAQQTIGC SDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGR DWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTR MEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSG FIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEF F" /label="NeoR" terminator 7922..8182 /modified_by="mamee" /label="t35S" primer complement(8236..8256) /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="95.24%" /Primary_Match="95.24%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" primer complement(8256..8277) /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="95.45%" /Primary_Match="95.45%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" rep_origin complement(8427..9015) /direction="LEFT" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" misc_feature complement(9256..10116) /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="99.88%" /Primary_Match="99.88%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" promoter complement(10117..10221) /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" ORIGIN 1 cgtctcagga ggtgcagcgt gacccggtcg tgcccctctc tagagataat gagcattgca 61 tgtctaagtt ataaaaaatt accacatatt ttttttgtca cacttgtttg aagtgcagtt 121 tatctatctt tatacatata tttaaacttt actctacgaa taatataatc tatagtacta 181 caataatatc agtgttttag agaatcatat aaatgaacag ttagacatgg tctaaaggac 241 aattgagtat tttgacaaca ggactctaca gttttatctt tttagtgtgc atgtgttctc 301 cttttttttt gcaaatagct tcacctatat aatacttcat ccattttatt agtacatcca 361 tttagggttt agggttaatg gtttttatag actaattttt ttagtacatc tattttattc 421 tattttagcc tctaaattaa gaaaactaaa actctatttt agttttttta tttaataatt 481 tagatataaa atagaataaa ataaagtgac taaaaattaa acaaataccc tttaagaaat 541 taaaaaaact aaggaaacat ttttcttgtt tcgagtagat aatgccagcc tgttaaacgc 601 cgtcgacgag tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga 661 agcagacggc acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac 721 cgttggactt gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc 781 cggcacggca ggcggcctcc tcctcctctc acggcaccgg cagctacggg ggattccttt 841 cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagacac cccctccaca 901 ccctctttcc ccaacctcgt gttgttcgga gcgcacacac acacaaccag atctccccca 961 aatccacccg tcggcacctc cgcttcaagg tacgccgctc gtcctccccc cccccccctc 1021 tctaccttct ctagatcggc gttccggtcc atggttaggg cccggtagtt ctacttctgt 1081 tcatgtttgt gttagatccg tgtttgtgtt agatccgtgc tgctagcgtt cgtacacgga 1141 tgcgacctgt acgtcagaca cgttctgatt gctaacttgc cagtgtttct ctttggggaa 1201 tcctgggatg gctctagccg ttccgcagac gggatcgatt tcatgatttt ttttgtttcg 1261 ttgcataggg tttggtttgc ccttttcctt tatttcaata tatgccgtgc acttgtttgt 1321 cgggtcatct tttcatgctt ttttttgtct tggttgtgat gatgtggtct ggttgggcgg 1381 tcgttctaga tcggagtaga attaattctg tttcaaacta cctggtggat ttattaattt 1441 tggatctgta tgtgtgtgcc atacatattc atagttacga attgaagatg atggatggaa 1501 atatcgatct aggataggta tacatgttga tgcgggtttt actgatgcat atacagagat 1561 gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg gcggtcgttc attcgttcta 1621 gatcggagta gaatactgtt tcaaactacc tggtgtattt attaattttg gaactgtatg 1681 tgtgtgtcat acatcttcat agttacgagt ttaagatgga tggaaatatc gatctaggat 1741 aggtatacat gttgatgtgg gttttactga tgcatataca tgatggcata tgcagcatct 1801 attcatatgc tctaaccttg agtacctatc tattataata aacaagtatg ttttataatt 1861 attttgatct tgatatactt ggatgatggc atatgcagca gctatatgtg gattttttta 1921 gccctgcctt catacgctat ttatttgctt ggtactgttt cttttgtcga tgctcaccct 1981 gttgtttggt gttacttctg caggtaacaa tggataagaa gtactctatc ggactcgata 2041 tcggaactaa ctctgtggga tgggctgtga tcaccgatga gtacaaggtg ccatctaaga 2101 agttcaaggt tctcggaaac accgataggc actctatcaa gaaaaacctt atcggtgctc 2161 tcctcttcga ttctggtgaa actgctgagg ctaccagact caagagaacc gctagaagaa 2221 ggtacaccag aagaaagaac aggatctgct acctccaaga gatcttctct aacgagatgg 2281 ctaaagtgga tgattcattc ttccacaggc tcgaagagtc attcctcgtg gaagaagata 2341 agaagcacga gaggcaccct atcttcggaa acatcgttga tgaggtggca taccacgaga 2401 agtaccctac tatctaccac ctcagaaaga agctcgttga ttctactgat aaggctgatc 2461 tcaggctcat ctacctcgct ctcgctcaca tgatcaagtt cagaggacac ttcctcatcg 2521 agggtgatct caaccctgat aactctgatg tggataagtt gttcatccag ctcgtgcaga 2581 cctacaacca gcttttcgaa gagaacccta tcaacgcttc aggtgtggat gctaaggcta 2641 tcctctctgc taggctctct aagtcaagaa ggcttgagaa cctcattgct cagctccctg 2701 gtgagaagaa gaacggactt ttcggaaact tgatcgctct ctctctcgga ctcaccccta 2761 acttcaagtc taacttcgat ctcgctgagg atgcaaagct ccagctctca aaggatacct 2821 acgatgatga tctcgataac ctcctcgctc agatcggaga tcagtacgct gatttgttcc 2881 tcgctgctaa gaacctctct gatgctatcc tcctcagtga tatcctcaga gtgaacaccg 2941 agatcaccaa ggctccactc tcagcttcta tgatcaagag atacgatgag caccaccagg 3001 atctcacact tctcaaggct cttgttagac agcagctccc agagaagtac aaagagattt 3061 tcttcgatca gtctaagaac ggatacgctg gttacatcga tggtggtgca tctcaagaag 3121 agttctacaa gttcatcaag cctatcctcg agaagatgga tggaaccgag gaactcctcg 3181 tgaagctcaa tagagaggat cttctcagaa agcagaggac cttcgataac ggatctatcc 3241 ctcatcagat ccacctcgga gagttgcacg ctatccttag aaggcaagag gatttctacc 3301 cattcctcaa ggataacagg gaaaagattg agaagattct caccttcaga atcccttact 3361 acgtgggacc tctcgctaga ggaaactcaa gattcgcttg gatgaccaga aagtctgagg 3421 aaaccatcac cccttggaac ttcgaagagg tggtggataa gggtgctagt gctcagtctt 3481 tcatcgagag gatgaccaac ttcgataaga accttccaaa cgagaaggtg ctccctaagc 3541 actctttgct ctacgagtac ttcaccgtgt acaacgagtt gaccaaggtt aagtacgtga 3601 ccgagggaat gaggaagcct gcttttttgt caggtgagca aaagaaggct atcgttgatc 3661 tcttgttcaa gaccaacaga aaggtgaccg tgaagcagct caaagaggat tacttcaaga 3721 aaatcgagtg cttcgattca gttgagattt ctggtgttga ggataggttc aacgcatctc 3781 tcggaaccta ccacgatctc ctcaagatca ttaaggataa ggatttcttg gataacgagg 3841 aaaacgagga tatcttggag gatatcgttc ttaccctcac cctctttgaa gatagagaga 3901 tgattgaaga aaggctcaag acctacgctc atctcttcga tgataaggtg atgaagcagt 3961 tgaagagaag aagatacact ggttggggaa ggctctcaag aaagctcatt aacggaatca 4021 gggataagca gtctggaaag acaatccttg atttcctcaa gtctgatgga ttcgctaaca 4081 gaaacttcat gcagctcatc cacgatgatt ctctcacctt taaagaggat atccagaagg 4141 ctcaggtttc aggacagggt gatagtctcc atgagcatat cgctaacctc gctggatctc 4201 ctgcaatcaa gaagggaatc ctccagactg tgaaggttgt ggatgagttg gtgaaggtga 4261 tgggaaggca taagcctgag aacatcgtga tcgaaatggc tagagagaac cagaccactc 4321 agaagggaca gaagaactct agggaaagga tgaagaggat cgaggaaggt atcaaagagc 4381 ttggatctca gatcctcaaa gagcaccctg ttgagaacac tcagctccag aatgagaagc 4441 tctacctcta ctacctccag aacggaaggg atatgtatgt ggatcaagag ttggatatca 4501 acaggctctc tgattacgat gttgatcata tcgtgccaca gtcattcttg aaggatgatt 4561 ctatcgataa caaggtgctc accaggtctg ataagaacag gggtaagagt gataacgtgc 4621 caagtgaaga ggttgtgaag aaaatgaaga actattggag gcagctcctc aacgctaagc 4681 tcatcactca gagaaagttc gataacttga ctaaggctga gaggggagga ctctctgaat 4741 tggataaggc aggattcatc aagaggcagc ttgtggaaac caggcagatc actaagcacg 4801 ttgcacagat cctcgattct aggatgaaca ccaagtacga tgagaacgat aagttgatca 4861 gggaagtgaa ggttatcacc ctcaagtcaa agctcgtgtc tgatttcaga aaggatttcc 4921 aattctacaa ggtgagggaa atcaacaact accaccacgc tcacgatgct taccttaacg 4981 ctgttgttgg aaccgctctc atcaagaagt atcctaagct cgagtcagag ttcgtgtacg 5041 gtgattacaa ggtgtacgat gtgaggaaga tgatcgctaa gtctgagcaa gagatcggaa 5101 aggctaccgc taagtatttc ttctactcta acatcatgaa tttcttcaag accgagatta 5161 ccctcgctaa cggtgagatc agaaagaggc cactcatcga gacaaacggt gaaacaggtg 5221 agatcgtgtg ggataaggga agggatttcg ctaccgttag aaaggtgctc tctatgccac 5281 aggtgaacat cgttaagaaa accgaggtgc agaccggtgg attctctaaa gagtctatcc 5341 tccctaagag gaactctgat aagctcattg ctaggaagaa ggattgggac cctaagaaat 5401 acggtggttt cgattctcct accgtggctt actctgttct cgttgtggct aaggttgaga 5461 agggaaagag taagaagctc aagtctgtta aggaacttct cggaatcact atcatggaaa 5521 ggtcatcttt cgagaagaac ccaatcgatt tcctcgaggc taagggatac aaagaggtta 5581 agaaggatct catcatcaag ctcccaaagt actcactctt cgaactcgag aacggtagaa 5641 agaggatgct cgcttctgct ggtgagcttc aaaagggaaa cgagcttgct ctcccatcta 5701 agtacgttaa ctttctttac ctcgcttctc actacgagaa gttgaaggga tctccagaag 5761 ataacgagca gaagcaactt ttcgttgagc agcacaagca ctacttggat gagatcatcg 5821 agcagatctc tgagttctct aaaagggtga tcctcgctga tgcaaacctc gataaggtgt 5881 tgtctgctta caacaagcac agagataagc ctatcaggga acaggcagag aacatcatcc 5941 atctcttcac ccttaccaac ctcggtgctc ctgctgcttt caagtacttc gatacaacca 6001 tcgataggaa gagatacacc tctaccaaag aagtgctcga tgctaccctc atccatcagt 6061 ctatcactgg actctacgag actaggatcg atctctcaca gctcggtggt gattcaaggg 6121 ctgatcctaa gaagaagagg aaggtttgag cttatatgtc aacagtgaga aactgttcgc 6181 attttccgtt ttgcttcttt ctttctattc aatgtatgtt gttggattcc agttgaattt 6241 attatgagaa ctaataataa tagtaataat catttgtttc tttactaatt tgcattttca 6301 catatgattt ctggtgcata tcataatttt cattccacca atattaattt ccccattcaa 6361 gttacttatg aaatagaaat cctcttctcc gactacttta tttgtccgaa agtcttgtgg 6421 ctgctatata acgctgtcat gagacgaatt cgaattccca tggagtcaaa gattcaaata 6481 gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga 6541 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacgct tgtctactcc 6601 aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg 6661 gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag 6721 atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc 6781 gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc 6841 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc 6901 actgacgtaa gggatgacgc ataacccgac gattttaagg aagacgctgc ctctttataa 6961 agaagtccat ttcatctgga gaggacaggg cacgcaccag aaagctacag cctacctcaa 7021 tacatcccta gaaaccgaga agattagacc acccagtatg gagaacatag aaaaattgct 7081 catgcaggaa aaaattctca tgctcgaact cgatttagtc agggcaaaaa taagtcttga 7141 cggactgcac gcaggatcac ccgccgcgtg ggtggagagg ctttttggtt acgattgggc 7201 acagcagacg ataggatgta gcgacgcagc agtttttcga ctctctgcgc agggcaggcc 7261 cgtcttgttt gtaaagaccg atttaagcgg agcgttaaat gagcttcagg atgaagctgc 7321 gaggttgtca tggttggcga ccacgggcgt gccatgcgcg gcagtcttgg atgtagtcac 7381 agaagcaggg agggattggt tgcttttggg ggaggtcccg ggtcaggatc ttctatcctc 7441 tcacttggcc ccggccgaga aagtatctat aatggctgac gccatgagaa gactacatac 7501 gttagacccg gcaacatgcc catttgacca tcaagcaaag caccgtatag agcgagcgcg 7561 taccaggatg gaagcaggcc ttgttgacca agatgattta gacgaagaac accaaggact 7621 ggcccctgcg gagttattcg cccgacttaa agcacgaatg ccggatggag aggatctggt 7681 cgtcacacac ggtgacgctt gcttacctaa tatcatggtc gagaacgggc gttttagcgg 7741 gtttattgac tgcggacgtt tgggagtcgc agacaggtac caggatattg ccttagccac 7801 acgagatata gcggaagaat tgggagggga gtgggcagat aggtttctcg tgctatatgg 7861 tatagccgcg cccgatagcc agcgaatagc cttctaccgt ttattagacg aattttttta 7921 ggcgggaccc aagcttcgac ggatctcgac ctgcaggcat gcccgctgaa atcaccagtc 7981 tctctctaca aatctatctc tctctataat aatgtgtgag tagttcccag ataagggaat 8041 tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt 8101 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagggg 8161 taccgagctc gaattcgcgg ccaggctagg tggaggctca gtgatgataa gtctgcgatg 8221 gtggatgcat gtgtcatggt catagctgtt tcctgtgtga aattgttatc cgctcagagg 8281 gcacaatcct attccgcgct atccgacaat ctccaagaca ttaggtggag ttcagttcgg 8341 cgtatggcat atgtcgctgg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 8401 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 8461 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 8521 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 8581 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 8641 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 8701 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 8761 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 8821 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 8881 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 8941 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 9001 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tctattcaac 9061 aaagccgccg tcccgtcaag tcagcgtaaa tgggtagggg gcttcaaatc gtcctcgtga 9121 taccaattcg gagcctgctt ttttgtacaa acttgttgat aatggcaatt caaggatctt 9181 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 9241 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 9301 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 9361 cttaccatct ggccccagtg ctgcaatgat accgcgagag ccacgctcac cggctccaga 9421 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 9481 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 9541 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 9601 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 9661 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 9721 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 9781 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 9841 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 9901 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 9961 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 10021 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 10081 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 10141 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 10201 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccagatacct gaaacaaaac 10261 ccatcgtacg gccaaggaag tctccaataa ctgtgatcca ccacaagcgc cagggttttc 10321 ccagtcacga cgttgtaaaa cgacggccag tcatgcataa tccgcacgca tctggaataa 10381 ggaagtgcca ttccgcctga ccttaacgaa tt //