LOCUS pDGB3_α2-pZmUbi-pcoCas9-tRbcS3a 12988 bp DNA circular SYN 06-NOV-2024 DEFINITION a2_pZmUbi-pcoCas9-tRbcS3a. ACCESSION VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid. REFERENCE 1 (bases 1 to 12988) AUTHORS SnapGene License TITLE Direct Submission JOURNAL Exported Feb 6, 2023 from SnapGene 6.2.1 https://www.snapgene.com FEATURES Location/Qualifiers misc_feature 1..11 /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" promoter 16..2007 /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pZmUBIL.2" /Transferred_Similarity="99.75%" /Primary_Match="99.75%; Adjusted-Interval-0:0/0; ZmUBIL.2 (pZmUBIL.2)" /Alternative_Match="99.75%; Adjusted-Interval-0:0/-1; ZmUBIL (pZmUBIL)" /label="ZmUBIL.2" TATA_signal 881..889 /note="/vntifkey=41" /label="TATA\box" 5'UTR 911..993 /note="/vntifkey=52" /label="5'UTR" TATA_signal 911 /note="/vntifkey=41" /label="+1" intron 994..2007 /created_by="lapau" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pZmUBIL.2" /Transferred_Similarity="99.61%" /Primary_Match="99.61%; Adjusted-Interval-0:0/0; ZmUBI intron (pZmUBIL.2)" /label="ZmUBI intron" Polymorphism 1407..1410 /note="/vntifkey=62" /label CDS 2014..6129 /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AtCas9" /Transferred_Similarity="100.00%" /Adjusted_Transferred_Similarity="99.13%" /Primary_Match="100.00%; Adjusted-Interval-0:0/-36 (adjusted to 99.13%; Adjusted-Interval-0:0/0); AtCas9" /Alternative_Match="99.98%; Adjusted-Interval-0:0/-36 (adjusted to 99.11%; Adjusted-Interval-0:0/0); AtCas9-D10A" /Alternative_Match="99.95%; Adjusted-Interval-0:0/-36 (adjusted to 99.08%; Adjusted-Interval-0:0/0); AtCas9-H840A" /Alternative_Match="99.93%; Adjusted-Interval-0:0/-36 (adjusted to 99.06%; Adjusted-Interval-0:0/0); AtCas9-D10A,H840A" /Alternative_Match="100.00%; Adjusted-Interval-0:3/-36 (adjusted to 99.06%; Adjusted-Interval-0:0/0); SpCas9" /Alternative_Match="99.93%; Adjusted-Interval-0:3/-36 (adjusted to 98.99%; Adjusted-Interval-0:0/0); SpCas9(D10A)" /Alternative_Match="99.93%; Adjusted-Interval-0:3/-36 (adjusted to 98.99%; Adjusted-Interval-0:0/0); SpCas9(H840A)" /Alternative_Match="99.85%; Adjusted-Interval-0:3/-36 (adjusted to 98.91%; Adjusted-Interval-0:0/0); SpCas9(D10A,H840A)" /label="AtCas9" NLS 6130..6150 /created_by="wacae" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="NLS SV40" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /label="SV40" terminator 6158..6626 /created_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Pea3AT" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Pea3AT" /label="Pea3AT" misc_feature complement(6627..6637) /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" T-DNA_border 6644..6668 /note="right border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="RB T-DNA repeat" regulatory 7969..8598 /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" regulatory 9027..10099 /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" rep_origin 10165..10359 /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label="pVS1 oriV" regulatory 10733..10993 /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" rep_origin complement(11029..11617) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pColE1 ori" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pColE1 ori" /Alternative_Match="99.83%; Adjusted-Interval-0:0/0; pUC ori" /label="pColE1 ori" misc_feature complement(11705..12520) /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="98.53%" /Primary_Match="98.53%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" T-DNA_border 12953..12977 /note="left border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="LB T-DNA repeat" ORIGIN 1 cgtctcagtc aggaggtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat 61 tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc 121 agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt 181 actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa 241 ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt 301 tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca 361 tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta catctatttt 421 attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat 481 aatttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag 541 aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa 601 acgccgtcga cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa 661 gcgaagcaga cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct 721 ccaccgttgg acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt 781 gagccggcac ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc 841 ctttcccacc gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc 901 cacaccctct ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc 961 cccaaatcca cccgtcggca cctccgcttc aaggtacgcc gctcgtcctc cccccccccc 1021 cctctctacc ttctctagat cggcgttccg gtccatggtt agggcccggt agttctactt 1081 ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca 1141 cggatgcgac ctgtacgtca gacacgttct gattgctaac ttgccagtgt ttctctttgg 1201 ggaatcctgg gatggctcta gccgttccgc agacgggatc gatttcatga ttttttttgt 1261 ttcgttgcat agggtttggt ttgccctttt cctttatttc aatatatgcc gtgcacttgt 1321 ttgtcgggtc atcttttcat gctttttttt gtcttggttg tgatgatgtg gtctggttgg 1381 gcggtcgttc tagatcggag tagaattaat tctgtttcaa actacctggt ggatttatta 1441 attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa gatgatggat 1501 ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat gcatatacag 1561 agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt 1621 tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat tttggaactg 1681 tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa tatcgatcta 1741 ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg catatgcagc 1801 atctattcat atgctctaac cttgagtacc tatctattat aataaacaag tatgttttat 1861 aattattttg atcttgatat acttggatga tggcatatgc agcagctata tgtggatttt 1921 tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg tcgatgctca 1981 ccctgttgtt tggtgttact tctgcaggta acaatggata agaagtactc tatcggactc 2041 gatatcggaa ctaactctgt gggatgggct gtgatcaccg atgagtacaa ggtgccatct 2101 aagaagttca aggttctcgg aaacaccgat aggcactcta tcaagaaaaa ccttatcggt 2161 gctctcctct tcgattctgg tgaaactgct gaggctacca gactcaagag aaccgctaga 2221 agaaggtaca ccagaagaaa gaacaggatc tgctacctcc aagagatctt ctctaacgag 2281 atggctaaag tggatgattc attcttccac aggctcgaag agtcattcct cgtggaagaa 2341 gataagaagc acgagaggca ccctatcttc ggaaacatcg ttgatgaggt ggcataccac 2401 gagaagtacc ctactatcta ccacctcaga aagaagctcg ttgattctac tgataaggct 2461 gatctcaggc tcatctacct cgctctcgct cacatgatca agttcagagg acacttcctc 2521 atcgagggtg atctcaaccc tgataactct gatgtggata agttgttcat ccagctcgtg 2581 cagacctaca accagctttt cgaagagaac cctatcaacg cttcaggtgt ggatgctaag 2641 gctatcctct ctgctaggct ctctaagtca agaaggcttg agaacctcat tgctcagctc 2701 cctggtgaga agaagaacgg acttttcgga aacttgatcg ctctctctct cggactcacc 2761 cctaacttca agtctaactt cgatctcgct gaggatgcaa agctccagct ctcaaaggat 2821 acctacgatg atgatctcga taacctcctc gctcagatcg gagatcagta cgctgatttg 2881 ttcctcgctg ctaagaacct ctctgatgct atcctcctca gtgatatcct cagagtgaac 2941 accgagatca ccaaggctcc actctcagct tctatgatca agagatacga tgagcaccac 3001 caggatctca cacttctcaa ggctcttgtt agacagcagc tcccagagaa gtacaaagag 3061 attttcttcg atcagtctaa gaacggatac gctggttaca tcgatggtgg tgcatctcaa 3121 gaagagttct acaagttcat caagcctatc ctcgagaaga tggatggaac cgaggaactc 3181 ctcgtgaagc tcaatagaga ggatcttctc agaaagcaga ggaccttcga taacggatct 3241 atccctcatc agatccacct cggagagttg cacgctatcc ttagaaggca agaggatttc 3301 tacccattcc tcaaggataa cagggaaaag attgagaaga ttctcacctt cagaatccct 3361 tactacgtgg gacctctcgc tagaggaaac tcaagattcg cttggatgac cagaaagtct 3421 gaggaaacca tcaccccttg gaacttcgaa gaggtggtgg ataagggtgc tagtgctcag 3481 tctttcatcg agaggatgac caacttcgat aagaaccttc caaacgagaa ggtgctccct 3541 aagcactctt tgctctacga gtacttcacc gtgtacaacg agttgaccaa ggttaagtac 3601 gtgaccgagg gaatgaggaa gcctgctttt ttgtcaggtg agcaaaagaa ggctatcgtt 3661 gatctcttgt tcaagaccaa cagaaaggtg accgtgaagc agctcaaaga ggattacttc 3721 aagaaaatcg agtgcttcga ttcagttgag atttctggtg ttgaggatag gttcaacgca 3781 tctctcggaa cctaccacga tctcctcaag atcattaagg ataaggattt cttggataac 3841 gaggaaaacg aggatatctt ggaggatatc gttcttaccc tcaccctctt tgaagataga 3901 gagatgattg aagaaaggct caagacctac gctcatctct tcgatgataa ggtgatgaag 3961 cagttgaaga gaagaagata cactggttgg ggaaggctct caagaaagct cattaacgga 4021 atcagggata agcagtctgg aaagacaatc cttgatttcc tcaagtctga tggattcgct 4081 aacagaaact tcatgcagct catccacgat gattctctca cctttaaaga ggatatccag 4141 aaggctcagg tttcaggaca gggtgatagt ctccatgagc atatcgctaa cctcgctgga 4201 tctcctgcaa tcaagaaggg aatcctccag actgtgaagg ttgtggatga gttggtgaag 4261 gtgatgggaa ggcataagcc tgagaacatc gtgatcgaaa tggctagaga gaaccagacc 4321 actcagaagg gacagaagaa ctctagggaa aggatgaaga ggatcgagga aggtatcaaa 4381 gagcttggat ctcagatcct caaagagcac cctgttgaga acactcagct ccagaatgag 4441 aagctctacc tctactacct ccagaacgga agggatatgt atgtggatca agagttggat 4501 atcaacaggc tctctgatta cgatgttgat catatcgtgc cacagtcatt cttgaaggat 4561 gattctatcg ataacaaggt gctcaccagg tctgataaga acaggggtaa gagtgataac 4621 gtgccaagtg aagaggttgt gaagaaaatg aagaactatt ggaggcagct cctcaacgct 4681 aagctcatca ctcagagaaa gttcgataac ttgactaagg ctgagagggg aggactctct 4741 gaattggata aggcaggatt catcaagagg cagcttgtgg aaaccaggca gatcactaag 4801 cacgttgcac agatcctcga ttctaggatg aacaccaagt acgatgagaa cgataagttg 4861 atcagggaag tgaaggttat caccctcaag tcaaagctcg tgtctgattt cagaaaggat 4921 ttccaattct acaaggtgag ggaaatcaac aactaccacc acgctcacga tgcttacctt 4981 aacgctgttg ttggaaccgc tctcatcaag aagtatccta agctcgagtc agagttcgtg 5041 tacggtgatt acaaggtgta cgatgtgagg aagatgatcg ctaagtctga gcaagagatc 5101 ggaaaggcta ccgctaagta tttcttctac tctaacatca tgaatttctt caagaccgag 5161 attaccctcg ctaacggtga gatcagaaag aggccactca tcgagacaaa cggtgaaaca 5221 ggtgagatcg tgtgggataa gggaagggat ttcgctaccg ttagaaaggt gctctctatg 5281 ccacaggtga acatcgttaa gaaaaccgag gtgcagaccg gtggattctc taaagagtct 5341 atcctcccta agaggaactc tgataagctc attgctagga agaaggattg ggaccctaag 5401 aaatacggtg gtttcgattc tcctaccgtg gcttactctg ttctcgttgt ggctaaggtt 5461 gagaagggaa agagtaagaa gctcaagtct gttaaggaac ttctcggaat cactatcatg 5521 gaaaggtcat ctttcgagaa gaacccaatc gatttcctcg aggctaaggg atacaaagag 5581 gttaagaagg atctcatcat caagctccca aagtactcac tcttcgaact cgagaacggt 5641 agaaagagga tgctcgcttc tgctggtgag cttcaaaagg gaaacgagct tgctctccca 5701 tctaagtacg ttaactttct ttacctcgct tctcactacg agaagttgaa gggatctcca 5761 gaagataacg agcagaagca acttttcgtt gagcagcaca agcactactt ggatgagatc 5821 atcgagcaga tctctgagtt ctctaaaagg gtgatcctcg ctgatgcaaa cctcgataag 5881 gtgttgtctg cttacaacaa gcacagagat aagcctatca gggaacaggc agagaacatc 5941 atccatctct tcacccttac caacctcggt gctcctgctg ctttcaagta cttcgataca 6001 accatcgata ggaagagata cacctctacc aaagaagtgc tcgatgctac cctcatccat 6061 cagtctatca ctggactcta cgagactagg atcgatctct cacagctcgg tggtgattca 6121 agggctgatc ctaagaagaa gaggaaggtt tgagcttcag gcctcccagc tttcgtccgt 6181 atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc ccacacacca 6241 gaatcctact aagtttgagt attatggcat tggaaaagct gttttcttct atcatttgtt 6301 ctgcttgtaa tttactgtgt tctttcagtt tttgttttcg gacatcaaaa tgcaaatgga 6361 tggataagag ttaataaatg atatggtcct tttgttcatt ctcaaattat tattatctgt 6421 tgtttttact ttaatgggtt gaatttaagt aagaaaggaa ctaacagtgt gatattaagg 6481 tgcaatgtta gacatataaa acagtctttc acctctcttt ggttatgtct tgaattggtt 6541 tgtttcttca cttatctgtg taatcaagtt tactatgagt ctatgatcaa gtaattatgc 6601 aatcaagtta agtacagtat aggcttcgct tgagacgaag ctttgacagg atatattggc 6661 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa 6721 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc 6781 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc 6841 agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc 6901 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg 6961 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg 7021 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg 7081 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg 7141 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc 7201 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg 7261 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt 7321 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga 7381 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc 7441 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg 7501 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg 7561 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg 7621 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca 7681 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc 7741 cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg cggctgcatg aaatcctggc 7801 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga 7861 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc 7921 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc 7981 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc 8041 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc 8101 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg 8161 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg 8221 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg 8281 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag 8341 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa 8401 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt 8461 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt 8521 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt 8581 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc 8641 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag 8701 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga 8761 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc 8821 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga 8881 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga 8941 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact 9001 ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat 9061 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg 9121 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat 9181 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa 9241 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag 9301 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga 9361 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat 9421 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat 9481 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc 9541 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga 9601 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa 9661 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa 9721 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga 9781 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga 9841 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc 9901 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt 9961 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga 10021 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga 10081 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga 10141 aaaaggtcga aaaggactct ttcctgtgga tagcacgtac attgggaacc caaagccgta 10201 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca 10261 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa 10321 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc 10381 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc 10441 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct 10501 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac 10561 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggtga 10621 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 10681 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 10741 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 10801 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc 10861 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 10921 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 10981 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 11041 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 11101 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 11161 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 11221 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 11281 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 11341 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 11401 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 11461 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 11521 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 11581 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 11641 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag 11701 gtgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat 11761 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt 11821 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac 11881 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga 11941 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag 12001 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg 12061 attgcgcctg agcgagtcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa 12121 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag 12181 gatattcttc taatacctgg aatgctgttt tccctgggat cgcagtggtg agtaaccatg 12241 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc 12301 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca 12361 gaaacaactc tggcgcatcg ggcttcccat acaatcggta gattgtcgca cctgattgcc 12421 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc 12481 gcggccttga gcaagacgtt tcccgttgaa tatggctcat aacagaactt attatttcct 12541 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc 12601 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg 12661 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca 12721 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 12781 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 12841 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 12901 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 12961 tatattgtgg tgtaaacata acaagctt //