LOCUS pDGB3_α1-pEC-pcoCas9-tRbcSE9 12509 bp DNA circular SYN 06-NOV-2024 DEFINITION a1_proEggCell-pcoCas9-tRbcSE9. ACCESSION VERSION . KEYWORDS pDGB3 alpha-1 - proEggCell - pcoCas9 - trbcSE9. SOURCE synthetic DNA construct ORGANISM recombinant plasmid. REFERENCE 1 (bases 1 to 12509) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported Feb 6, 2023 from SnapGene 6.2.1 https://www.snapgene.com FEATURES Location/Qualifiers misc_feature 1..11 /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" promoter 12..1363 /note="EC1.2 enhancer and EC1.1 promoter from Wang et al., 2015" /modified_by="mamee" /label="EggCell promoter" CDS 1371..5486 /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AtCas9" /Transferred_Similarity="100.00%" /Adjusted_Transferred_Similarity="99.13%" /Primary_Match="100.00%; Adjusted-Interval-0:0/-36 (adjusted to 99.13%; Adjusted-Interval-0:0/0); AtCas9" /Alternative_Match="99.98%; Adjusted-Interval-0:0/-36 (adjusted to 99.11%; Adjusted-Interval-0:0/0); AtCas9-D10A" /Alternative_Match="99.95%; Adjusted-Interval-0:0/-36 (adjusted to 99.08%; Adjusted-Interval-0:0/0); AtCas9-H840A" /Alternative_Match="99.93%; Adjusted-Interval-0:0/-36 (adjusted to 99.06%; Adjusted-Interval-0:0/0); AtCas9-D10A,H840A" /Alternative_Match="100.00%; Adjusted-Interval-0:3/-36 (adjusted to 99.06%; Adjusted-Interval-0:0/0); SpCas9" /Alternative_Match="99.93%; Adjusted-Interval-0:3/-36 (adjusted to 98.99%; Adjusted-Interval-0:0/0); SpCas9(D10A)" /Alternative_Match="99.93%; Adjusted-Interval-0:3/-36 (adjusted to 98.99%; Adjusted-Interval-0:0/0); SpCas9(H840A)" /Alternative_Match="99.85%; Adjusted-Interval-0:3/-36 (adjusted to 98.91%; Adjusted-Interval-0:0/0); SpCas9(D10A,H840A)" /modified_by="mamee" /label="AtCas9" NLS 5487..5507 /created_by="wacae" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="NLS SV40" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /label="SV40" terminator 5515..6145 /note="note=""Fragment extracted from fragmentTRbcSE9.txt.xdna [639 nt] : (#BsmBI[5] / #BsmBI[660])" /modified_by="mamee" /label="tRbcSE9" misc_feature complement(6150..6160) /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" T-DNA_border 6166..6190 /note="right border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="RB T-DNA repeat" regulatory 7491..8120 /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" regulatory 8549..9621 /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" rep_origin 9687..9881 /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label="pVS1 oriV" regulatory 10255..10515 /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" rep_origin complement(10551..11139) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pColE1 ori" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pColE1 ori" /Alternative_Match="99.83%; Adjusted-Interval-0:0/0; pUC ori" /label="pColE1 ori" misc_feature complement(11227..12042) /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="98.53%" /Primary_Match="98.53%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" T-DNA_border 12475..12499 /note="left border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="LB T-DNA repeat" ORIGIN 1 cgtctcagga ggaataaaag catttgcgtt tggtttatca ttgcgtttat acaaggacag 61 agatccactg agctggaata gcttaaaacc attatcagaa caaaataaac cattttttgt 121 taagaatcag agcatagtaa acaacagaaa caacctaaga gaggtaactt gtccaagaag 181 atagctaatt atatctattt tataaaagtt atcatagttt gtaagtcaca aaagatgcaa 241 ataacagaga aactaggaga cttgagaata tacattcttg tatatttgta ttcgagattg 301 tgaaaatttg accataagtt taaattctta aaaagatata tctgatctag gtgatggtta 361 tagactgtaa ttttaccaca tgtttaatga tggatagtga cacacatgac acatcgacaa 421 cactatagca tcttatttag attacaacat gaaatttttc tgtaatacat gtctttgtac 481 ataatttaaa agtaattcct aagaaatata tttatacaag gagtttaaag aaaacatagc 541 atatagttca atgagtagta aaaaccatat acagtatata gcataaagtt caatgagttt 601 attacaaaag cattggttca ctttctgtaa cacgacgtta aaccttcgac tccaatagga 661 gcgctactga ttcaacatgc caatatatac taaatacgtt tctacagtca aatgctttaa 721 cgtttcatga ttaagtgact atttaccgtc aatcctttcc cattcctccc actaatccaa 781 ctttttaatt actcttaaat caccactaag ctagtaacgc ctatcatgaa ttagctctac 841 taaatctagc aacctttcaa atttgcagta ttgcaggtgt ctctgtgtct ttaaaatagt 901 tgccttatga tttcttcggt ttcaagatga tcaaatagtt atagatttca tgctcacaca 961 tgctcattag atgtgtacat actttactta cccaaatcta ttttctcgca aagattttga 1021 tggtaaagct gatttggttc tattgaacta aatcaaacga gtttcagact gagtgattct 1081 aatccggccc attagcccct aaacagaccc actaattacg cagcttttaa tagagtaatt 1141 acacctagtt tacccactaa accactaagc actaattatc tcacaatcta atgagcttcc 1201 ctcgtaatta cttgggcttt cactctacca tttatttgta acagtcaagt ctctactgtc 1261 tctatataaa ctctctaaag ttaacacaca attctcatca caaacaaatc aaccaaagca 1321 acttctactc tttcttcttt cgaccttatc aatctgttga gaaatctaga atggataaga 1381 agtactctat cggactcgat atcggaacta actctgtggg atgggctgtg atcaccgatg 1441 agtacaaggt gccatctaag aagttcaagg ttctcggaaa caccgatagg cactctatca 1501 agaaaaacct tatcggtgct ctcctcttcg attctggtga aactgctgag gctaccagac 1561 tcaagagaac cgctagaaga aggtacacca gaagaaagaa caggatctgc tacctccaag 1621 agatcttctc taacgagatg gctaaagtgg atgattcatt cttccacagg ctcgaagagt 1681 cattcctcgt ggaagaagat aagaagcacg agaggcaccc tatcttcgga aacatcgttg 1741 atgaggtggc ataccacgag aagtacccta ctatctacca cctcagaaag aagctcgttg 1801 attctactga taaggctgat ctcaggctca tctacctcgc tctcgctcac atgatcaagt 1861 tcagaggaca cttcctcatc gagggtgatc tcaaccctga taactctgat gtggataagt 1921 tgttcatcca gctcgtgcag acctacaacc agcttttcga agagaaccct atcaacgctt 1981 caggtgtgga tgctaaggct atcctctctg ctaggctctc taagtcaaga aggcttgaga 2041 acctcattgc tcagctccct ggtgagaaga agaacggact tttcggaaac ttgatcgctc 2101 tctctctcgg actcacccct aacttcaagt ctaacttcga tctcgctgag gatgcaaagc 2161 tccagctctc aaaggatacc tacgatgatg atctcgataa cctcctcgct cagatcggag 2221 atcagtacgc tgatttgttc ctcgctgcta agaacctctc tgatgctatc ctcctcagtg 2281 atatcctcag agtgaacacc gagatcacca aggctccact ctcagcttct atgatcaaga 2341 gatacgatga gcaccaccag gatctcacac ttctcaaggc tcttgttaga cagcagctcc 2401 cagagaagta caaagagatt ttcttcgatc agtctaagaa cggatacgct ggttacatcg 2461 atggtggtgc atctcaagaa gagttctaca agttcatcaa gcctatcctc gagaagatgg 2521 atggaaccga ggaactcctc gtgaagctca atagagagga tcttctcaga aagcagagga 2581 ccttcgataa cggatctatc cctcatcaga tccacctcgg agagttgcac gctatcctta 2641 gaaggcaaga ggatttctac ccattcctca aggataacag ggaaaagatt gagaagattc 2701 tcaccttcag aatcccttac tacgtgggac ctctcgctag aggaaactca agattcgctt 2761 ggatgaccag aaagtctgag gaaaccatca ccccttggaa cttcgaagag gtggtggata 2821 agggtgctag tgctcagtct ttcatcgaga ggatgaccaa cttcgataag aaccttccaa 2881 acgagaaggt gctccctaag cactctttgc tctacgagta cttcaccgtg tacaacgagt 2941 tgaccaaggt taagtacgtg accgagggaa tgaggaagcc tgcttttttg tcaggtgagc 3001 aaaagaaggc tatcgttgat ctcttgttca agaccaacag aaaggtgacc gtgaagcagc 3061 tcaaagagga ttacttcaag aaaatcgagt gcttcgattc agttgagatt tctggtgttg 3121 aggataggtt caacgcatct ctcggaacct accacgatct cctcaagatc attaaggata 3181 aggatttctt ggataacgag gaaaacgagg atatcttgga ggatatcgtt cttaccctca 3241 ccctctttga agatagagag atgattgaag aaaggctcaa gacctacgct catctcttcg 3301 atgataaggt gatgaagcag ttgaagagaa gaagatacac tggttgggga aggctctcaa 3361 gaaagctcat taacggaatc agggataagc agtctggaaa gacaatcctt gatttcctca 3421 agtctgatgg attcgctaac agaaacttca tgcagctcat ccacgatgat tctctcacct 3481 ttaaagagga tatccagaag gctcaggttt caggacaggg tgatagtctc catgagcata 3541 tcgctaacct cgctggatct cctgcaatca agaagggaat cctccagact gtgaaggttg 3601 tggatgagtt ggtgaaggtg atgggaaggc ataagcctga gaacatcgtg atcgaaatgg 3661 ctagagagaa ccagaccact cagaagggac agaagaactc tagggaaagg atgaagagga 3721 tcgaggaagg tatcaaagag cttggatctc agatcctcaa agagcaccct gttgagaaca 3781 ctcagctcca gaatgagaag ctctacctct actacctcca gaacggaagg gatatgtatg 3841 tggatcaaga gttggatatc aacaggctct ctgattacga tgttgatcat atcgtgccac 3901 agtcattctt gaaggatgat tctatcgata acaaggtgct caccaggtct gataagaaca 3961 ggggtaagag tgataacgtg ccaagtgaag aggttgtgaa gaaaatgaag aactattgga 4021 ggcagctcct caacgctaag ctcatcactc agagaaagtt cgataacttg actaaggctg 4081 agaggggagg actctctgaa ttggataagg caggattcat caagaggcag cttgtggaaa 4141 ccaggcagat cactaagcac gttgcacaga tcctcgattc taggatgaac accaagtacg 4201 atgagaacga taagttgatc agggaagtga aggttatcac cctcaagtca aagctcgtgt 4261 ctgatttcag aaaggatttc caattctaca aggtgaggga aatcaacaac taccaccacg 4321 ctcacgatgc ttaccttaac gctgttgttg gaaccgctct catcaagaag tatcctaagc 4381 tcgagtcaga gttcgtgtac ggtgattaca aggtgtacga tgtgaggaag atgatcgcta 4441 agtctgagca agagatcgga aaggctaccg ctaagtattt cttctactct aacatcatga 4501 atttcttcaa gaccgagatt accctcgcta acggtgagat cagaaagagg ccactcatcg 4561 agacaaacgg tgaaacaggt gagatcgtgt gggataaggg aagggatttc gctaccgtta 4621 gaaaggtgct ctctatgcca caggtgaaca tcgttaagaa aaccgaggtg cagaccggtg 4681 gattctctaa agagtctatc ctccctaaga ggaactctga taagctcatt gctaggaaga 4741 aggattggga ccctaagaaa tacggtggtt tcgattctcc taccgtggct tactctgttc 4801 tcgttgtggc taaggttgag aagggaaaga gtaagaagct caagtctgtt aaggaacttc 4861 tcggaatcac tatcatggaa aggtcatctt tcgagaagaa cccaatcgat ttcctcgagg 4921 ctaagggata caaagaggtt aagaaggatc tcatcatcaa gctcccaaag tactcactct 4981 tcgaactcga gaacggtaga aagaggatgc tcgcttctgc tggtgagctt caaaagggaa 5041 acgagcttgc tctcccatct aagtacgtta actttcttta cctcgcttct cactacgaga 5101 agttgaaggg atctccagaa gataacgagc agaagcaact tttcgttgag cagcacaagc 5161 actacttgga tgagatcatc gagcagatct ctgagttctc taaaagggtg atcctcgctg 5221 atgcaaacct cgataaggtg ttgtctgctt acaacaagca cagagataag cctatcaggg 5281 aacaggcaga gaacatcatc catctcttca cccttaccaa cctcggtgct cctgctgctt 5341 tcaagtactt cgatacaacc atcgatagga agagatacac ctctaccaaa gaagtgctcg 5401 atgctaccct catccatcag tctatcactg gactctacga gactaggatc gatctctcac 5461 agctcggtgg tgattcaagg gctgatccta agaagaagag gaaggtttga gcttgctttc 5521 gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt cattgcgcac 5581 acaccagaat cctactgagt ttgagtatta tggcattggg aaaactgttt ttcttgtacc 5641 atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa 5701 tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa 5761 tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca 5821 aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat 5881 gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac aaatatattt 5941 tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg tgttttagac 6001 atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta atcattgctt 6061 tataattata gttatactca tggatttgta gttgagtatg aaaatatttt ttaatgcatt 6121 ttatgacttg ccaattgatt gacaacgctg tcatgagacg aattctgaca ggatatattg 6181 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg 6241 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga 6301 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt 6361 gcagccgtca tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc 6421 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg 6481 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta 6541 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc 6601 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct 6661 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg 6721 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc 6781 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt 6841 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg 6901 gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac 6961 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga 7021 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga 7081 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga 7141 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac 7201 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg 7261 gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg tgcggctgca tgaaatcctg 7321 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc 7381 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc 7441 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta 7501 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc 7561 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg 7621 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc 7681 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag 7741 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc 7801 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc 7861 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca 7921 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc 7981 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg 8041 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa 8101 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac 8161 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc 8221 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct 8281 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc 8341 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag 8401 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg 8461 gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca 8521 ctggaacccc caagcccgag gaatcggcgt gacggtcgca aaccatccgg cccggtacaa 8581 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag 8641 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga 8701 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc 8761 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat 8821 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc 8881 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc 8941 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc 9001 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt 9061 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta 9121 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag 9181 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta 9241 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc 9301 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc 9361 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa 9421 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag 9481 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag 9541 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc 9601 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg 9661 gaaaaaggtc gaaaaggact ctttcctgtg gatagcacgt acattgggaa cccaaagccg 9721 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca 9781 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta 9841 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca 9901 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct 9961 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat 10021 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc 10081 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccggt 10141 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 10201 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 10261 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg 10321 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc 10381 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 10441 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 10501 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 10561 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 10621 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 10681 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 10741 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 10801 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 10861 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 10921 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 10981 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 11041 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 11101 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 11161 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct 11221 aggtgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt 11281 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca 11341 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat 11401 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt 11461 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac 11521 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg 11581 tgattgcgcc tgagcgagtc gaaatacgcg atcgctgtta aaaggacaat tacaaacagg 11641 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc 11701 aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg tgagtaacca 11761 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag 11821 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt 11881 cagaaacaac tctggcgcat cgggcttccc atacaatcgg tagattgtcg cacctgattg 11941 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa 12001 tcgcggcctt gagcaagacg tttcccgttg aatatggctc ataacagaac ttattatttc 12061 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact 12121 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt 12181 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat 12241 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc 12301 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa 12361 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt 12421 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag 12481 gatatattgt ggtgtaaaca taacgaatt //