LOCUS pGGIB-U1-pZmUBI.2-zCas9-tNOS-U2 9009 bp DNA circular UNA 05-FEB-2024 DEFINITION . ACCESSION urn.local.psbuser.mb-hau6k64 VERSION urn.local.psbuser.mb-hau6k64 KEYWORDS . SOURCE ORGANISM . COMMENT ##Clone_number-START## Clone number :: C319 ##Clone_number-END## ##E.coli_Glycerol_Stock-START## E.coli Glycerol Stock :: CG311 ##E.coli_Glycerol_Stock-END## FEATURES Location/Qualifiers misc_difference 1..18 /created_by="laurenspauwels" /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="I-SceI site" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; I-SceI site" /label="I-SceI site" misc_feature 19..58 /created_by="lapau" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="U1" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; U1" /label="U1" /note="Geneious type: Golden Gibson Cloning site" promoter 71..2058 /vntifkey=29 /modified_by="laurenspauwels" /modified_by="mamee" /label="pZmUBI" intron 1049..2058 /created_by="lapau" /note="Derived using Geneious Prime 2020.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pZmUBIL.2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBI intron (pZmUBIL.2)" /label="ZmUBI intron" tag 2066..2131 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /Transferred_Translation="DYKDHDGDYKDHDIDYKDDDDK" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on protein similarity" /Transferred_From="3xFLAG" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3xFLAG" /label="3xFLAG" NLS 2138..2158 /created_by="wacae" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="NLS SV40 - short" /Transferred_Similarity="95.24%" /Primary_Match="95.24%; Adjusted-Interval-0:0/0; SV40 (NLS SV40 - short)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /label="SV40" CDS 2183..6283 /Transferred_From="ZmCas9" /Transferred_Similarity="100.00%" /label="zCAS9" NLS 6284..6331 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on protein similarity" /Transferred_From="NLS Nucleoplasmin" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Nucleoplasmin (NLS Nucleoplasmin)" /label="Nucleoplasmin" terminator 6340..6607 /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (NOST)" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="2 Hygromycin" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (2 Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (3 Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (1 Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (tNOS v2)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:13/-2; tNOS (tNOS v1)" /label="tNOS" misc_feature 6618..6657 /created_by="lapau" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="U2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; U2" /label="U2" /note="Geneious type: Golden Gibson Cloning site" misc_difference complement(6658..6675) /created_by="laurenspauwels" /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="I-SceI site" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; I-SceI site" /label="I-SceI site" primer complement(6703..6723) /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 6784..6834 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 6835..7644 /modified_by="wacae" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 7791..8379 /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 8978..8998 /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 attaccctgt tatccctaca ttactcgcat ccattctcag gctgtctcgt ctcgtctcct 61 cgagatacct gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat 121 gtctaagtta taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt 181 atctatcttt atacatatat ttaaacttta ctctacgaat aatataatct atagtactac 241 aataatatca gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca 301 attgagtatt ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc 361 tttttttttg caaatagctt cacctatata atacttcatc cattttatta gtacatccat 421 ttagggttta gggttaatgg tttttataga ctaatttttt tagtacatct attttattct 481 attttagcct ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt 541 agatataaaa tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt 601 aaaaaaacta aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc 661 gtcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa 721 gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc 781 gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc 841 ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg gattcctttc 901 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac 961 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa 1021 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct 1081 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt 1141 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat 1201 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat 1261 cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt 1321 tgcatagggt ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc 1381 gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt 1441 cgttctagat cggagtagaa ttctgtttca aactacctgg tggatttatt aattttggat 1501 ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc 1561 gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt 1621 ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg 1681 gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt 1741 gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta 1801 tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca 1861 tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt 1921 gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct 1981 gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt 2041 ttggtgttac ttctgcaggg ctatggatta caaggaccac gacggggatt acaaggacca 2101 cgacattgat tacaaggatg atgatgacaa gatggctccg aagaagaaga ggaaggttgg 2161 catccacggg gtgccagctg ctgacaagaa gtactcgatc ggcctcgata ttgggactaa 2221 ctctgttggc tgggccgtga tcaccgacga gtacaaggtg ccctcaaaga agttcaaggt 2281 cctgggcaac accgatcggc attccatcaa gaagaatctc attggcgctc tcctgttcga 2341 cagcggcgag acggctgagg ctacgcggct caagcgcacc gcccgcaggc ggtacacgcg 2401 caggaagaat cgcatctgct acctgcagga gattttctcc aacgagatgg cgaaggttga 2461 cgattctttc ttccacaggc tggaggagtc attcctcgtg gaggaggata agaagcacga 2521 gcggcatcca atcttcggca acattgtcga cgaggttgcc taccacgaga agtaccctac 2581 gatctaccat ctgcggaaga agctcgtgga ctccacagat aaggcggacc tccgcctgat 2641 ctacctcgct ctggcccaca tgattaagtt caggggccat ttcctgatcg agggggatct 2701 caacccggac aatagcgatg ttgacaagct gttcatccag ctcgtgcaga cgtacaacca 2761 gctcttcgag gagaacccca ttaatgcgtc aggcgtcgac gcgaaggcta tcctgtccgc 2821 taggctctcg aagtctcggc gcctcgagaa cctgatcgcc cagctgccgg gcgagaagaa 2881 gaacggcctg ttcgggaatc tcattgcgct cagcctgggg ctcacgccca acttcaagtc 2941 gaatttcgat ctcgctgagg acgccaagct gcagctctcc aaggacacat acgacgatga 3001 cctggataac ctcctggccc agatcggcga tcagtacgcg gacctgttcc tcgctgccaa 3061 gaatctgtcg gacgccatcc tcctgtctga tattctcagg gtgaacaccg agattacgaa 3121 ggctccgctc tcagcctcca tgatcaagcg ctacgacgag caccatcagg atctgaccct 3181 cctgaaggcg ctggtcaggc agcagctccc cgagaagtac aaggagatct tcttcgatca 3241 gtcgaagaac ggctacgctg ggtacattga cggcggggcc tctcaggagg agttctacaa 3301 gttcatcaag ccgattctgg agaagatgga cggcacggag gagctgctgg tgaagctcaa 3361 tcgcgaggac ctcctgagga agcagcggac attcgataac ggcagcatcc cacaccagat 3421 tcatctcggg gagctgcacg ctatcctgag gaggcaggag gacttctacc ctttcctcaa 3481 ggataaccgc gagaagatcg agaagattct gactttcagg atcccgtact acgtcggccc 3541 actcgctagg ggcaactccc gcttcgcttg gatgacccgc aagtcagagg agacgatcac 3601 gccgtggaac ttcgaggagg tggtcgacaa gggcgctagc gctcagtcgt tcatcgagag 3661 gatgacgaat ttcgacaaga acctgccaaa tgagaaggtg ctccctaagc actcgctcct 3721 gtacgagtac ttcacagtct acaacgagct gactaaggtg aagtatgtga ccgagggcat 3781 gaggaagccg gctttcctgt ctggggagca gaagaaggcc atcgtggacc tcctgttcaa 3841 gaccaaccgg aaggtcacgg ttaagcagct caaggaggac tacttcaaga agattgagtg 3901 cttcgattcg gtcgagatct ctggcgttga ggaccgcttc aacgcctccc tggggaccta 3961 ccacgatctc ctgaagatca ttaaggataa ggacttcctg gacaacgagg agaatgagga 4021 tatcctcgag gacattgtgc tgacactcac tctgttcgag gaccgggaga tgatcgagga 4081 gcgcctgaag acttacgccc atctcttcga tgacaaggtc atgaagcagc tcaagaggag 4141 gaggtacacc ggctggggga ggctgagcag gaagctcatc aacggcattc gggacaagca 4201 gtccgggaag acgatcctcg acttcctgaa gagcgatggc ttcgcgaacc gcaatttcat 4261 gcagctgatt cacgatgaca gcctcacatt caaggaggat atccagaagg ctcaggtgag 4321 cggccagggg gactcgctgc acgagcatat cgcgaacctc gctggctcgc cagctatcaa 4381 gaaggggatt ctgcagaccg tgaaggttgt ggacgagctg gtgaaggtca tgggcaggca 4441 caagcctgag aacatcgtca ttgagatggc ccgggagaat cagaccacgc agaagggcca 4501 gaagaactca cgcgagagga tgaagaggat cgaggagggc attaaggagc tggggtccca 4561 gatcctcaag gagcacccgg tggagaacac gcagctgcag aatgagaagc tctacctgta 4621 ctacctccag aatggccgcg atatgtatgt ggaccaggag ctggatatta acaggctcag 4681 cgattacgac gtcgatcata tcgttccaca gtcattcctg aaggatgact ccattgacaa 4741 caaggtcctc accaggtcgg acaagaaccg gggcaagtct gataatgttc cttcagagga 4801 ggtcgttaag aagatgaaga actactggcg ccagctcctg aatgccaagc tgatcacgca 4861 gcggaagttc gataacctca caaaggctga gaggggcggg ctctctgagc tggacaaggc 4921 gggcttcatc aagaggcagc tggtcgagac acggcagatc actaagcacg ttgcgcagat 4981 tctcgactca cggatgaaca ctaagtacga tgagaatgac aagctgatcc gcgaggtgaa 5041 ggtcatcacc ctgaagtcaa agctcgtctc cgacttcagg aaggatttcc agttctacaa 5101 ggttcgggag atcaacaatt accaccatgc ccatgacgcg tacctgaacg cggtggtcgg 5161 cacagctctg atcaagaagt acccaaagct cgagagcgag ttcgtgtacg gggactacaa 5221 ggtttacgat gtgaggaaga tgatcgccaa gtcggagcag gagattggca aggctaccgc 5281 caagtacttc ttctactcta acattatgaa tttcttcaag acagagatca ctctggccaa 5341 tggcgagatc cggaagcgcc ccctcatcga gacgaacggc gagacggggg agatcgtgtg 5401 ggacaagggc agggatttcg cgaccgtcag gaaggttctc tccatgccac aagtgaatat 5461 cgtcaagaag acagaggtcc agactggcgg gttctctaag gagtcaattc tgcctaagcg 5521 gaacagcgac aagctcatcg cccgcaagaa ggactgggat ccgaagaagt acggcgggtt 5581 cgacagcccc actgtggcct actcggtcct ggttgtggcg aaggttgaga agggcaagtc 5641 caagaagctc aagagcgtga aggagctgct ggggatcacg attatggagc gctccagctt 5701 cgagaagaac ccgatcgatt tcctggaggc gaagggctac aaggaggtga agaaggacct 5761 gatcattaag ctccccaagt actcactctt cgagctggag aacggcagga agcggatgct 5821 ggcttccgct ggcgagctgc agaaggggaa cgagctggct ctgccgtcca agtatgtgaa 5881 cttcctctac ctggcctccc actacgagaa gctcaagggc agccccgagg acaacgagca 5941 gaagcagctg ttcgtcgagc agcacaagca ttacctcgac gagatcattg agcagatttc 6001 cgagttctcc aagcgcgtga tcctggccga cgcgaatctg gataaggtcc tctccgcgta 6061 caacaagcac cgcgacaagc caatcaggga gcaggctgag aatatcattc atctcttcac 6121 cctgacgaac ctcggcgccc ctgctgcttt caagtacttc gacacaacta tcgatcgcaa 6181 gaggtacaca agcactaagg aggtcctgga cgcgaccctc atccaccagt cgattaccgg 6241 cctctacgag acgcgcatcg acctgtctca gctcgggggc gacaagcggc cagcggcgac 6301 gaagaaggcg gggcaggcga agaagaagaa gtgattcagc tagagtcaag cagatcgttc 6361 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat 6421 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt 6481 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga 6541 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact 6601 agatcgagta ttctagagct gggagttcgt agacggaaac aaacgcagaa tccaagctag 6661 ggataacagg gtaatgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc 6721 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata 6781 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc 6841 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat 6901 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat 6961 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat 7021 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc 7081 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa 7141 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg 7201 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat 7261 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt 7321 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa 7381 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt 7441 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac 7501 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag 7561 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat 7621 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc 7681 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac 7741 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 7801 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 7861 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 7921 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 7981 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 8041 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 8101 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 8161 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 8221 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 8281 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 8341 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 8401 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 8461 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 8521 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 8581 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 8641 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta 8701 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt 8761 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc 8821 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg 8881 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg 8941 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag 9001 ctcgggccc //