LOCUS pFASTRK-RPS5A-(start)BP-Cas9-BP*-G7T-Multiplex1 21255 bp DNA circular UNA 22-DEC-2023 DEFINITION . ACCESSION urn.local...43-h5acahy VERSION urn.local...43-h5acahy KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers Promoter 3..277 /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; pc (GmR)" /label="P1" misc_feature 278..1069 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" rep_origin 1315..1903 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.83%" /Primary_Match="99.83%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" regulatory complement(1939..2199) /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:30/150; bom" /label="pBR322 bom" rep_origin 2573..2767 /note="origin of replication from Pseudomonas pVS1 plasmid" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 oriV" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV" /label="pVS1 oriV" regulatory complement(2833..3906) /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" regulatory complement(4335..4964) /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" T-DNA_border 6150..6380 /modified_by="wacae" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="RB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; RB" /label="RB" promoter 6386..8085 /Transferred_From="pRPS5A" /Transferred_Similarity="100.00%" /modified_by="wacae" /label="pRPS5A" Linker 8090..8113 /created_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker" /label="Linker" NLS 8123..8176 /created_by="wacae" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="NLS BP" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP (NLS BP)" /Alternative_Match="100.00%; Adjusted-Interval-0:33/0; SV40 (NLS SV40 - short)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; BP (NLS BP)" /Alternative_Match="100.00%; Adjusted-Interval-0:33/0; SV40 (NLS SV40)" /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP* (NLS BP*)" /label="BP" CDS 8183..12286 /Transferred_From="Cas9PTA" /Transferred_Similarity="100.00%" /label="CAS9" NLS 12293..12343 /created_by="wacae" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="NLS BP*" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (NLS BP*)" /Alternative_Match="100.00%; Adjusted-Interval-0:30/0; SV40 (NLS SV40 - short)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; BP* (NLS BP*)" /Alternative_Match="100.00%; Adjusted-Interval-0:30/0; SV40 (NLS SV40)" /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP (NLS BP)" /label="BP*" Linker 12348..12393 /created_by="wacae" /Transferred_From="Linker I" /Transferred_Similarity="100.00%" /modified_by="wacae" /label="Linker" terminator 12398..12669 /vntifkey=43 /label="G7T" misc_feature 12697^12698 /Original_Bases="ACCT" /label="ACCT" /note="Geneious type: Editing History Deletion" promoter 12700..13087 /modified_by="thjac" /label="AtU6-26" scaffold 13108..13190 /%_Identity=100 /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT" /annotation_group="Scaffold: 451 -> 533" /modified_by="thjac" /label="Scaffold" promoter 13197..13584 /modified_by="thjac" /label="AtU6-26" scaffold 13605..13687 /%_Identity=100 /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT" /annotation_group="Scaffold: 451 -> 533" /modified_by="thjac" /label="Scaffold" promoter 13694..14081 /modified_by="thjac" /label="AtU6-26" Scaffold 14102..14184 /Mismatches=0 /%_Identity=100 /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT" /annotation_group="Scaffold: 451 -> 533" /modified_by="wacae" /Transferred_From="Scaffold, gRNA scaffold" /Transferred_Similarity="100.00%" /label="Scaffold" Linker 14189..14212 /created_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker" /label="Linker" promoter 14219..14606 /vntifkey=29 /modified_by="thjac" /modified_by="wacae" /Transferred_From="AtU6-26" /Transferred_Similarity="100.00%" /label="AtU6-26" scaffold 14627..14709 /%_Identity=100 /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT" /annotation_group="Scaffold: 451 -> 533" /modified_by="thjac" /label="Scaffold" promoter 14716..15103 /modified_by="thjac" /label="AtU6-26" scaffold 15124..15206 /%_Identity=100 /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT" /annotation_group="Scaffold: 451 -> 533" /modified_by="thjac" /label="Scaffold" promoter 15213..15600 /modified_by="thjac" /label="AtU6-26" scaffold 15621..15703 /%_Identity=100 /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT" /annotation_group="Scaffold: 451 -> 533" /modified_by="thjac" /label="Scaffold" promoter 15710..16097 /vntifkey=29 /modified_by="thjac" /label="AtU6-26" scaffold 16118..16200 /%_Identity=100 /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT" /annotation_group="Scaffold: 451 -> 533" /modified_by="thjac" /label="Scaffold" misc_feature 16204^16205 /Original_Bases="GTAT" /label="GTAT" /note="Geneious type: Editing History Deletion" promoter 16258..17700 /label="OLE1P" CDS join(17705..18057,18298..18463) /modified_by="thjac" /label="OLE1" Intron 18058..18297 /Mismatches=0 /%_Identity=100 /Motif="GTAAGCACACATTTATCATCTTACTTCATAATTTTGTGCAATATGTGCAT GCATGTGTTGAGCCAGTAGCTTTGGATCAATTTTTTTGGTCGAATAACAAATGTAACA ATAAGAAATTGCAAATTCTAGGGAACATTTGGTTAACTAAATACGAAATTTGACCTAG CTAGCTTGAATGTGTCTGTGTATATCATCTATATAGGTAAAATGCTTGGTATGATACC TATTGATTGTGAATAG" /annotation_group="Intron: 4,340 -> 4,579" /label="Intron" CDS 18470..19180 /note="added by Rabuo" /label="mRuby3 (without T38V)" Linker 19185..19230 /created_by="wacae" /Color="#ff9911" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker" /label="Linker" terminator 19235..19502 /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (NOST)" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="2 Hygromycin" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (2 Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (3 Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (1 Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (tNOS v2)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:13/-2; tNOS (tNOS v1)" /label="tNOS" Plant_Selection 19514..20902 /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="3 Kanamycin" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (3 Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:15/0; Kana (Kanamycin)" /label="Kana" promoter 19514..19801 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pNOS" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="3 Kanamycin" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (3 Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (1 Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:15/0; pNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:15/0; pNOS (pNOS v2)" /Alternative_Match="100.00%; Adjusted-Interval-0:15/0; pNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:15/0; pNOS (Kanamycin)" /Alternative_Match="97.89%; Adjusted-Interval-0:4/0; pNOS (2 Hygromycin)" /Alternative_Match="97.85%; Adjusted-Interval-0:9/0; pNOS (pNOS v1)" /label="pNOS" CDS 19814..20608 /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NptII" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; NptII" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="3 Kanamycin" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; NptII (3 Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; NptII" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; NptII (Kanamycin)" /Alternative_Match="98.87%; Adjusted-Interval-0:-3/0 (adjusted to 98.87%; Adjusted-Interval-0:0/0); NptII (pDe-Cas9-D10A - Kanamycin)" /label="NptII" terminator 20635..20902 /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (NOST)" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="2 Hygromycin" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (2 Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (3 Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (1 Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (tNOS v2)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:13/-2; tNOS (tNOS v1)" /label="tNOS" T-DNA_border 20923..21255 /modified_by="wacae" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="LB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB" /label="LB" ORIGIN 1 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat 61 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc 121 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa 181 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac 241 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga 301 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt 361 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat 421 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa 481 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt 541 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca 601 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga 661 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc 721 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga 781 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct 841 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc 901 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag 961 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga 1021 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag 1081 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta 1141 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg 1201 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg 1261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 1321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 1381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 1441 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 1501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 1561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 1621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 1681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 1741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 1801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 1861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 1921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 1981 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 2041 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta 2101 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat 2161 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc 2221 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc 2281 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt 2341 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag 2401 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt 2461 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga 2521 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca 2581 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc 2641 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg 2701 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct 2761 atccacagga aagagagctt ttcgaccttt ttcccctgct agggcaattt gccctagcat 2821 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat 2881 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt 2941 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact 3001 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc 3061 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt 3121 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat 3181 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc 3241 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc 3301 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc 3361 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg 3421 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat 3481 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac 3541 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag 3601 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat 3661 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc 3721 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc 3781 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt 3841 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc 3901 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc 3961 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg 4021 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc 4081 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt 4141 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa 4201 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt 4261 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc 4321 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg 4381 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct 4441 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc 4501 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc 4561 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg 4621 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg 4681 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc 4741 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc 4801 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact 4861 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc 4921 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta 4981 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact 5041 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg 5101 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg 5161 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt 5221 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg 5281 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc 5341 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag 5401 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc 5461 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt 5521 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg 5581 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt 5641 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc 5701 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac 5761 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc 5821 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt 5881 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct 5941 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa 6001 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga 6061 cttgtgcgac atgtcgtttt cagaagaggg ctgcactgaa cgtcagaagc cgactgcact 6121 atagcagcgg aggggttgga tcaaagtact ttaaagtact ttaaagtact ttaaagtact 6181 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc 6241 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac 6301 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg 6361 atccaagctc aagctaagct tacctgaact gtaggagctc cccataatcg tgagtagata 6421 tattactcaa cttttgattc gctatttgca gtgcacctgt ggcgttcatc acatcttttg 6481 tgacactgtt tgcactggtc attgctatta caaaggacct tcctgatgtt gaaggagatc 6541 gaaagtaagt aactgcacgc ataaccattt tctttccgct ctttggctca atccatttga 6601 cagtcaaaga caatgtttaa ccagctccgt ttgatatatt gtctttatgt gtttgttcaa 6661 gcatgtttag ttaatcatgc ctttgattga tcttgaatag gttccaaata tcaaccctgg 6721 caacaaaact tggagtgaga aacattgcat tcctcggttc tggacttctg ctagtaaatt 6781 atgtttcagc catatcacta gctttctaca tgcctcaggt gaattcatct atttccgtct 6841 taactatttc ggttaattaa agcacgaaca ccattactgc atgtagaagc ttgataaact 6901 atcgccacca atttattttt gttgcgatat tgttactttc ctcagtatgc agctttgaaa 6961 agaccaaccc tcttatcctt taacaatgaa caggttttta gaggtagctt gatgattcct 7021 gcacatgtga tcttggcttc aggcttaatt ttccaggtaa agcattatga gatactctta 7081 tatctcttac atacttttga gataatgcac aagaacttca taactatatg ctttagtttc 7141 tgcatttgac actgccaaat tcattaatct ctaatatctt tgttgttgat ctttggtaga 7201 catgggtact agaaaaagca aactacacca aggtaaaata cttttgtaca aacataaact 7261 cgttatcacg gaacatcaat ggagtgtata tctaacggag tgtagaaaca tttgattatt 7321 gcaggaagct atctcaggat attatcggtt tatatggaat ctcttctacg cagagtatct 7381 gttattcccc ttcctctagc tttcaatttc atggtgagga tatgcagttt tctttgtata 7441 tcattcttct tcttctttgt agcttggagt caaaatcggt tccttcatgt acatacatca 7501 aggatatgtc cttctgaatt tttatatctt gcaataaaaa tgcttgtacc aattgaaaca 7561 ccagcttttt gagttctatg atcactgact tggttctaac caaaaaaaaa aaaatgttta 7621 atttacatat ctaaaagtag gtttagggaa acctaaacag taaaatattt gtatattatt 7681 cgaatttcac tcatcataaa aacttaaatt gcaccataaa attttgtttt actattaatg 7741 atgtaatttg tgtaacttaa gataaaaata atattccgta agttaaccgg ctaaaaccac 7801 gtataaacca gggaacctgt taaaccggtt ctttactgga taaagaaatg aaagcccatg 7861 tagacagctc cattagagcc caaaccctaa atttctcatc tatataaaag gagtgacatt 7921 agggtttttg ttcgtcctct taaagcttct cgttttctct gccgtctctc tcattcgcgc 7981 gacgcaaacg atcttcaggt gatcttcttt ctccaaatcc tctctcataa ctctgatttc 8041 gtacttgtgt atttgagctc acgctctgtt tctctcacca cagccaacag tattcagtcg 8101 actggtacca acaggctcca tgaaacggac agccgacgga agcgagttcg agtcacctaa 8161 gaagaagagg aaggttggct cgatggataa gaagtactct atcggactcg atatcggaac 8221 taactctgtg ggatgggctg tgatcaccga tgagtacaag gtgccatcta agaagttcaa 8281 ggttctcgga aacaccgata ggcactctat caagaaaaac cttatcggtg ctctcctctt 8341 cgattctggt gaaactgctg aggctaccag actcaagaga accgctagaa gaaggtacac 8401 cagaagaaag aacaggatct gctacctcca agagatcttc tctaacgaga tggctaaagt 8461 ggatgattca ttcttccaca ggctcgaaga gtcattcctc gtggaagaag ataagaagca 8521 cgagaggcac cctatcttcg gaaacatcgt tgatgaggtg gcataccacg agaagtaccc 8581 tactatctac cacctcagaa agaagctcgt tgattctact gataaggctg atctcaggct 8641 catctacctc gctctcgctc acatgatcaa gttcagagga cacttcctca tcgagggtga 8701 tctcaaccct gataactctg atgtggataa gttgttcatc cagctcgtgc agacctacaa 8761 ccagcttttc gaagagaacc ctatcaacgc ttcaggtgtg gatgctaagg ctatcctctc 8821 tgctaggctc tctaagtcaa gaaggcttga gaacctcatt gctcagctcc ctggtgagaa 8881 gaagaacgga cttttcggaa acttgatcgc tctctctctc ggactcaccc ctaacttcaa 8941 gtctaacttc gatctcgctg aggatgcaaa gctccagctc tcaaaggata cctacgatga 9001 tgatctcgat aacctcctcg ctcagatcgg agatcagtac gctgatttgt tcctcgctgc 9061 taagaacctc tctgatgcta tcctcctcag tgatatcctc agagtgaaca ccgagatcac 9121 caaggctcca ctctcagctt ctatgatcaa gagatacgat gagcaccacc aggatctcac 9181 acttctcaag gctcttgtta gacagcagct cccagagaag tacaaagaga ttttcttcga 9241 tcagtctaag aacggatacg ctggttacat cgatggtggt gcatctcaag aagagttcta 9301 caagttcatc aagcctatcc tcgagaagat ggatggaacc gaggaactcc tcgtgaagct 9361 caatagagag gatcttctca gaaagcagag gaccttcgat aacggatcta tccctcatca 9421 gatccacctc ggagagttgc acgctatcct tagaaggcaa gaggatttct acccattcct 9481 caaggataac agggaaaaga ttgagaagat tctcaccttc agaatccctt actacgtggg 9541 acctctcgct agaggaaact caagattcgc ttggatgacc agaaagtctg aggaaaccat 9601 caccccttgg aacttcgaag aggtggtgga taagggtgct agtgctcagt ctttcatcga 9661 gaggatgacc aacttcgata agaaccttcc aaacgagaag gtgctcccta agcactcttt 9721 gctctacgag tacttcaccg tgtacaacga gttgaccaag gttaagtacg tgaccgaggg 9781 aatgaggaag cctgcttttt tgtcaggtga gcaaaagaag gctatcgttg atctcttgtt 9841 caagaccaac agaaaggtga ccgtgaagca gctcaaagag gattacttca agaaaatcga 9901 gtgcttcgat tcagttgaga tttctggtgt tgaggatagg ttcaacgcat ctctcggaac 9961 ctaccacgat ctcctcaaga tcattaagga taaggatttc ttggataacg aggaaaacga 10021 ggatatcttg gaggatatcg ttcttaccct caccctcttt gaagatagag agatgattga 10081 agaaaggctc aagacctacg ctcatctctt cgatgataag gtgatgaagc agttgaagag 10141 aagaagatac actggttggg gaaggctctc aagaaagctc attaacggaa tcagggataa 10201 gcagtctgga aagacaatcc ttgatttcct caagtctgat ggattcgcta acagaaactt 10261 catgcagctc atccacgatg attctctcac ctttaaagag gatatccaga aggctcaggt 10321 ttcaggacag ggtgatagtc tccatgagca tatcgctaac ctcgctggat ctcctgcaat 10381 caagaaggga atcctccaga ctgtgaaggt tgtggatgag ttggtgaagg tgatgggaag 10441 gcataagcct gagaacatcg tgatcgaaat ggctagagag aaccagacca ctcagaaggg 10501 acagaagaac tctagggaaa ggatgaagag gatcgaggaa ggtatcaaag agcttggatc 10561 tcagatcctc aaagagcacc ctgttgagaa cactcagctc cagaatgaga agctctacct 10621 ctactacctc cagaacggaa gggatatgta tgtggatcaa gagttggata tcaacaggct 10681 ctctgattac gatgttgatc atatcgtgcc acagtcattc ttgaaggatg attctatcga 10741 taacaaggtg ctcaccaggt ctgataagaa caggggtaag agtgataacg tgccaagtga 10801 agaggttgtg aagaaaatga agaactattg gaggcagctc ctcaacgcta agctcatcac 10861 tcagagaaag ttcgataact tgactaaggc tgagagggga ggactctctg aattggataa 10921 ggcaggattc atcaagaggc agcttgtgga aaccaggcag atcactaagc acgttgcaca 10981 gatcctcgat tctaggatga acaccaagta cgatgagaac gataagttga tcagggaagt 11041 gaaggttatc accctcaagt caaagctcgt gtctgatttc agaaaggatt tccaattcta 11101 caaggtgagg gaaatcaaca actaccacca cgctcacgat gcttacctta acgctgttgt 11161 tggaaccgct ctcatcaaga agtatcctaa gctcgagtca gagttcgtgt acggtgatta 11221 caaggtgtac gatgtgagga agatgatcgc taagtctgag caagagatcg gaaaggctac 11281 cgctaagtat ttcttctact ctaacatcat gaatttcttc aagaccgaga ttaccctcgc 11341 taacggtgag atcagaaaga ggccactcat cgagacaaac ggtgaaacag gtgagatcgt 11401 gtgggataag ggaagggatt tcgctaccgt tagaaaggtg ctctctatgc cacaggtgaa 11461 catcgttaag aaaaccgagg tgcagaccgg tggattctct aaagagtcta tcctccctaa 11521 gaggaactct gataagctca ttgctaggaa gaaggattgg gaccctaaga aatacggtgg 11581 tttcgattct cctaccgtgg cttactctgt tctcgttgtg gctaaggttg agaagggaaa 11641 gagtaagaag ctcaagtctg ttaaggaact tctcggaatc actatcatgg aaaggtcatc 11701 tttcgagaag aacccaatcg atttcctcga ggctaaggga tacaaagagg ttaagaagga 11761 tctcatcatc aagctcccaa agtactcact cttcgaactc gagaacggta gaaagaggat 11821 gctcgcttct gctggtgagc ttcaaaaggg aaacgagctt gctctcccat ctaagtacgt 11881 taactttctt tacctcgctt ctcactacga gaagttgaag ggatctccag aagataacga 11941 gcagaagcaa cttttcgttg agcagcacaa gcactacttg gatgagatca tcgagcagat 12001 ctctgagttc tctaaaaggg tgatcctcgc tgatgcaaac ctcgataagg tgttgtctgc 12061 ttacaacaag cacagagata agcctatcag ggaacaggca gagaacatca tccatctctt 12121 cacccttacc aacctcggtg ctcctgctgc tttcaagtac ttcgatacaa ccatcgatag 12181 gaagagatac acctctacca aagaagtgct cgatgctacc ctcatccatc agtctatcac 12241 tggactctac gagactagga tcgatctctc acagctcggt ggtgattcag gtaaaagaac 12301 cgccgacggc agcgaattcg agcctaagaa gaagaggaag gtttcaggtg gatcctagat 12361 aacctttacc ttcattccct gtaaattggt accctgcccc cgatgagcta agctagctat 12421 atcatcaatt tatgtattac acataatatc gcactcagtc tttcatctac ggcaatgtac 12481 cagctgatat aatcagttat tgaaatattt ctgaatttaa acttgcatca ataaatttat 12541 gtttttgctt ggactataat acctgacttg ttattttatc aataaatatt taaactatat 12601 ttctttcaag atgggaatta acatctacaa attgcctttt cttatcgacc atgtactcga 12661 cggccgagta ctataagcta ggtagtaact agtacctctc tttttttctt cttcttcgtt 12721 catacagttt ttttttgttt atcagcttac attttcttga accgtagctt tcgttttctt 12781 ctttttaact ttccattcgg agtttttgta tcttgtttca tagtttgtcc caggattaga 12841 atgattaggc atcgaacctt caagaatttg attgaataaa acatcttcat tcttaagata 12901 tgaagataat cttcaaaagg cccctgggaa tctgaaagaa gagaagcagg cccatttata 12961 tgggaaagaa caatagtatt tcttatatag gcccatttaa gttgaaaaca atcttcaaaa 13021 gtcccacatc gcttagataa gaaaacgaag ctgagtttat atacagctag agtcgaagta 13081 gtgattggga aaagttgtag actgagagtt ttagagctag aaatagcaag ttaaaataag 13141 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt aacactcttt 13201 ttttcttctt cttcgttcat acagtttttt tttgtttatc agcttacatt ttcttgaacc 13261 gtagctttcg ttttcttctt tttaactttc cattcggagt ttttgtatct tgtttcatag 13321 tttgtcccag gattagaatg attaggcatc gaaccttcaa gaatttgatt gaataaaaca 13381 tcttcattct taagatatga agataatctt caaaaggccc ctgggaatct gaaagaagag 13441 aagcaggccc atttatatgg gaaagaacaa tagtatttct tatataggcc catttaagtt 13501 gaaaacaatc ttcaaaagtc ccacatcgct tagataagaa aacgaagctg agtttatata 13561 cagctagagt cgaagtagtg attggactgg gacccgaacg aaccgtttta gagctagaaa 13621 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc 13681 tttttttggc tctctttttt tcttcttctt cgttcataca gttttttttt gtttatcagc 13741 ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat tcggagtttt 13801 tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa ccttcaagaa 13861 tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa aaggcccctg 13921 ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag tatttcttat 13981 ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag ataagaaaac 14041 gaagctgagt ttatatacag ctagagtcga agtagtgatt ggcgctgcac aacaatcttg 14101 ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag 14161 tggcaccgag tcggtgcttt ttttaacagt attcagtcga ctggtaccaa caggctctct 14221 ttttttcttc ttcttcgttc atacagtttt tttttgttta tcagcttaca ttttcttgaa 14281 ccgtagcttt cgttttcttc tttttaactt tccattcgga gtttttgtat cttgtttcat 14341 agtttgtccc aggattagaa tgattaggca tcgaaccttc aagaatttga ttgaataaaa 14401 catcttcatt cttaagatat gaagataatc ttcaaaaggc ccctgggaat ctgaaagaag 14461 agaagcaggc ccatttatat gggaaagaac aatagtattt cttatatagg cccatttaag 14521 ttgaaaacaa tcttcaaaag tcccacatcg cttagataag aaaacgaagc tgagtttata 14581 tacagctaga gtcgaagtag tgattgggcc aagccgagta tccatagttt tagagctaga 14641 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt 14701 gctttttttt cagctctttt tttcttcttc ttcgttcata cagttttttt ttgtttatca 14761 gcttacattt tcttgaaccg tagctttcgt tttcttcttt ttaactttcc attcggagtt 14821 tttgtatctt gtttcatagt ttgtcccagg attagaatga ttaggcatcg aaccttcaag 14881 aatttgattg aataaaacat cttcattctt aagatatgaa gataatcttc aaaaggcccc 14941 tgggaatctg aaagaagaga agcaggccca tttatatggg aaagaacaat agtatttctt 15001 atataggccc atttaagttg aaaacaatct tcaaaagtcc cacatcgctt agataagaaa 15061 acgaagctga gtttatatac agctagagtc gaagtagtga ttgctcagga tcaacaccga 15121 gcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa 15181 agtggcaccg agtcggtgct ttttttctgc ctcttttttt cttcttcttc gttcatacag 15241 tttttttttg tttatcagct tacattttct tgaaccgtag ctttcgtttt cttcttttta 15301 actttccatt cggagttttt gtatcttgtt tcatagtttg tcccaggatt agaatgatta 15361 ggcatcgaac cttcaagaat ttgattgaat aaaacatctt cattcttaag atatgaagat 15421 aatcttcaaa aggcccctgg gaatctgaaa gaagagaagc aggcccattt atatgggaaa 15481 gaacaatagt atttcttata taggcccatt taagttgaaa acaatcttca aaagtcccac 15541 atcgcttaga taagaaaacg aagctgagtt tatatacagc tagagtcgaa gtagtgattg 15601 gtgagctccc ggcggatcta gttttagagc tagaaatagc aagttaaaat aaggctagtc 15661 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttactactc tttttttctt 15721 cttcttcgtt catacagttt ttttttgttt atcagcttac attttcttga accgtagctt 15781 tcgttttctt ctttttaact ttccattcgg agtttttgta tcttgtttca tagtttgtcc 15841 caggattaga atgattaggc atcgaacctt caagaatttg attgaataaa acatcttcat 15901 tcttaagata tgaagataat cttcaaaagg cccctgggaa tctgaaagaa gagaagcagg 15961 cccatttata tgggaaagaa caatagtatt tcttatatag gcccatttaa gttgaaaaca 16021 atcttcaaaa gtcccacatc gcttagataa gaaaacgaag ctgagtttat atacagctag 16081 agtcgaagta gtgattgccc tgagaataaa ctcgtcggtt ttagagctag aaatagcaag 16141 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt 16201 gtatctagaa tcgcgtatgc gagtgtatct gcaggcatgc gacgtcgggc cctctagtac 16261 ttagatcaac acataaaagt tagtaagtga agaaccacaa caacaacact agattcatct 16321 tcaagtgtat gtaggtatag taacatgaac aagaacagac tcaagtacaa gatcgcatac 16381 gaaaatggaa atggcaatgt cacttccaca taatcaaaca cgaatcctca tatcaacaag 16441 gcctgagatt ctaactagct cataacaact tagccaatag ttacttgaga ctaccaaatg 16501 tatgtagaac taaagactaa gggacagaga gttcgtctaa acaggtgaat ctagtcgttg 16561 ttatctaata aacaattcag ccccaaatgc agaacacaca tagagctctc tattgattca 16621 aattacgatc tgatactgat aacgtctaga tttttagggt taaagcaatc aatcacctga 16681 cgattcaagg tggttggatc atgacgattc cagaaaacat caagcaagct ctcaaagcta 16741 cactctttgg gatcatactg aactctaaca acctcgttat gtcccgtagt gccagtacag 16801 acatcctcgt aactcggatt gtgcacgatg ccatggctat acccaacctc ggtcttggtc 16861 acaccaggaa ctctctggta agctagctcc actccccaga aacaaccggc gccaaattgc 16921 gcgaattgct gacctgaaga cggaacatca tcgtcgggtc cttgggcgat tgcggcggaa 16981 gatgggtcag cttgggcttg aggacgagac gcgaatccga gtctgttgaa aaggttgttc 17041 attggggatt tgtatacgga gattggtcgt cgagaggttt gagggaaagg acaaatgggt 17101 ttggctctgg agaaagagag tgcggcttta gagagagaat tgagaggttt agagagagat 17161 gcggcggcga tgagcggagg agagacgacg aggacctgca ttatcaaagc agtgacgtgg 17221 tgaaatttgg aacttttaag aggcagatag atttattatt tgtatccatt ttcttcattg 17281 ttctagaatg tcgcggaaca aattttaaaa ctaaatccta aatttttcta attttgttgc 17341 caatagtgga tatgtgggcc gtatagaagg aatctattga aggcccaaac ccatactgac 17401 gagcccaaag gttcgttttg cgttttatgt ttcggttcga tgccaacgcc acattctgag 17461 ctaggcaaaa aacaaacgtg tctttgaata gactcctctc gttaacacat gcagcggctg 17521 catggtgacg ccattaacac gtggcctaca attgcatgat gtctccattg acacgtgact 17581 tctcgtctcc tttcttaata tatctaacaa acactcctac ctcttccaaa atatatacac 17641 atctttttga tcaatctctc attcaaaatc tcattctctc tagtaaacaa gaacaaaaaa 17701 aacaatggcg gatacagcta gaggaaccca tcacgatatc atcggcagag atcagtaccc 17761 gatgatgggc cgagatcgag atcagtacca gatgtccgga cgaggatctg actactccaa 17821 gtctaggcag attgctaaag ctgcaactgc tgtcacagct ggtggttccc tccttgttct 17881 ctccagcctt acccttgttg gaactgtcat agctttgact gttgcaacac ctctgctcgt 17941 tatcttcagc ccaatccttg tcccggctct catcacagtt gcactcctca tcaccggttt 18001 tctttcctct ggagggtttg gcattgccgc tataaccgtt ttctcttgga tttacaagta 18061 agcacacatt tatcatctta cttcataatt ttgtgcaata tgtgcatgca tgtgttgagc 18121 cagtagcttt ggatcaattt ttttggtcga ataacaaatg taacaataag aaattgcaaa 18181 ttctagggaa catttggtta actaaatacg aaatttgacc tagctagctt gaatgtgtct 18241 gtgtatatca tctatatagg taaaatgctt ggtatgatac ctattgattg tgaataggta 18301 cgcaacggga gagcacccac agggatcaga caagttggac agtgcaagga tgaagttggg 18361 aagcaaagct caggatctga aagacagagc tcagtactac ggacagcaac atactggtgg 18421 ggaacatgac cgtgaccgta ctcgtggtgg ccagcacact actggctcca tggtgtctaa 18481 gggcgaagag ctgatcaagg aaaatatgcg tatgaaggtg gtcatggaag gttcggtcaa 18541 cggccaccaa ttcaaatgca caggtgaagg agaaggcaga ccgtacgagg gaactcaaac 18601 catgaggatc aaagtcatcg agggaggacc cctgccattt gcctttgaca ttcttgccac 18661 gtcgttcatg tatggcagcc gtacttttat caagtacccg gccgacatcc ctgatttctt 18721 taaacagtcc tttcctgagg gttttacttg ggaaagagtt acgagatacg aagatggtgg 18781 agtcgtcacc gtcacgcagg acaccagcct tgaggatggc gagctcgtct acaacgtcaa 18841 ggtcagaggg gtaaactttc cctccaatgg tcccgtgatg cagaagaaga ccaagggttg 18901 ggagcctaat acagagatga tgtatccagc agatggtggt ctgagaggat acactgacat 18961 cgcactgaaa gttgatggtg gtggccatct gcactgcaac ttcgtgacaa cttacaggtc 19021 aaaaaagacc gtcgggaaca tcaagatgcc cggtgtccat gccgttgatc accgcctgga 19081 aaggatcgag gagagtgaca atgaaaccta cgtagtgcaa cgcgaagtgg cagttgccaa 19141 atacagcaac cttggtggtg gcatggacga gctgtacaag tcaggtggat cctagataac 19201 ctttaccttc attccctgta aattggtacc ctgcctagag tcaagcagat cgttcaaaca 19261 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat 19321 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta 19381 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca 19441 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc 19501 gagtaccgcg aattatcgat catgagcgga gaattaaggg agtcacgtta tgacccccgc 19561 cgatgacgcg ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag 19621 ccactgagcc gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat 19681 tgcgcgttca aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg 19741 ctccactgac gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca 19801 actcgatcga ggcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 19861 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 19921 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 19981 cctgaatgaa ctccaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 20041 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 20101 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 20161 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 20221 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 20281 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 20341 gcggatgccc gacggcgagg atctcgtcgt gacccacggc gatgcctgct tgccgaatat 20401 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 20461 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 20521 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 20581 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcggact ctagctagag 20641 tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg 20701 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat 20761 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat 20821 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt 20881 gtcatctatg ttactagatc gaccggcatg caagctgata attcaattcg gcgttaattc 20941 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac 21001 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat 21061 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa 21121 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg 21181 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt 21241 tgctgcctgt gatca //