LOCUS p35S:FHA1-eGFP-HOTag6-p35S:SOMAshort-GFP-HOTag3 13233 bp DNA circular UNA 13-OCT-2023 DEFINITION . ACCESSION urn.local...yp-gvafmxg VERSION urn.local...yp-gvafmxg KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers Promoter 8..289 /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="96.81%" /Primary_Match="96.81%; Adjusted-Interval-0:0/0; P1 (SpR)" /Alternative_Match="89.66%; Adjusted-Interval-0:12/-241; pc (GmR)" /label="P1" misc_feature 290..1081 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" rep_origin 1325..1913 /snapgene_direction="RIGHT" /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" regulatory complement(1949..2209) /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:30/150; bom" /label="pBR322 bom" rep_origin 2583..2777 /note="origin of replication from Pseudomonas pVS1 plasmid" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 oriV" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV" /label="pVS1 oriV" regulatory complement(2843..3915) /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" regulatory complement(4344..4973) /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" T-DNA_border 6129..6358 /modified_by="wacae" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="RB" /Transferred_Similarity="91.77%" /Primary_Match="91.77%; Adjusted-Interval-0:0/0; RB" /label="RB" promoter 6370..7233 /regulatory_class="promoter" /gene="CaMV 35S" /note="internal BsaI recognition site removed" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCaMV 35S - Golden Gate" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Golden Gate)" /Alternative_Match="96.00%; Adjusted-Interval-0:505/-13; CaMV 35S Promoter (pDe-Cas9 - Basta)" /label="p35S" regulatory 7238..7247 /note="vertebrate consensus sequence for strong initiation of translation (Koza k, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 7247..7672 /codon_start=1 /transl_table=1 /translation="EKFSQEQIGENIVCRVICTTGQIPIRDLSADISQVLKEKRSIKK VWTFGRNPACDYHLGNISRLSNKHFQILLGEDGNLLLNDISTNGTWLNGQKVEKNSNQ LLSQGDEITVGVGVESDILSLVIFINDKFKQCLEQNKVDR" /label="FHA" CDS 7679..8395 /codon_start=1 /note="mammalian codon-optimized" /note="/vntifkey=4" /product="enhanced GFP" /transl_table=1 /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK" /label="EGFP" Linker 8396..8482 /modified_by="mamee" /label="Linker2" CDS 8483..8584 /note="/vntifkey=21" /modified_by="mamee" /label="HO-Tag6" terminator 8589..8828 /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="6 35ST" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S (6 35ST)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; t35S (t35S v1)" /Alternative_Match="100.00%; Adjusted-Interval-0:14/0; t35S (t35S v2)" /Alternative_Match="100.00%; Adjusted-Interval-0:164/-6; CaMV Poly(A) signal (pDe-Cas9 - Basta)" /Alternative_Match="91.43%; Adjusted-Interval-0:75/10; CaMV poly(A) signal (pDe-Cas9-D10A - Kanamycin)" /label="t35S" terminator 8833..9100 /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (NOST)" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="2 Hygromycin" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (2 Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (3 Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (1 Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (tNOS v2)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:13/-2; tNOS (tNOS v1)" /label="tNOS" Linker 9105..9124 /note="/created_by=wacae" /modified_by="mamee" /label="Linker" promoter 9129..9992 /regulatory_class="promoter" /gene="CaMV 35S" /note="internal BsaI recognition site removed" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCaMV 35S - Golden Gate" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Golden Gate)" /Alternative_Match="96.00%; Adjusted-Interval-0:505/-13; CaMV 35S Promoter (pDe-Cas9 - Basta)" /label="p35S" regulatory 9997..10006 /note="vertebrate consensus sequence for strong initiation of translation (Koza k, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 10006..10050 /codon_start=1 /transl_table=1 /translation="GTPRDHHNAKARACL" /label="SOMA short" Linker 10057..10104 /note="/vntifkey=21" /modified_by="mamee" /label="Linker" CDS 10105..10821 /codon_start=1 /note="mammalian codon-optimized" /note="/vntifkey=4" /product="enhanced GFP" /transl_table=1 /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK" /label="EGFP" Linker 10822..10911 /modified_by="mamee" /label="Linker2" CDS 10912..11001 /note="/vntifkey=21" /modified_by="mamee" /label="HO-Tag3" terminator 11009..11248 /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="6 35ST" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S (6 35ST)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; t35S (t35S v1)" /Alternative_Match="100.00%; Adjusted-Interval-0:14/0; t35S (t35S v2)" /Alternative_Match="100.00%; Adjusted-Interval-0:164/-6; CaMV Poly(A) signal (pDe-Cas9 - Basta)" /Alternative_Match="91.43%; Adjusted-Interval-0:75/10; CaMV poly(A) signal (pDe-Cas9-D10A - Kanamycin)" /label="t35S" terminator 11253..11520 /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (NOST)" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="2 Hygromycin" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (2 Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (3 Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (1 Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (tNOS v2)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:13/-2; tNOS (tNOS v1)" /label="tNOS" Plant_Selection 11525..12898 /Color="#006600" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Kanamycin" /Transferred_Similarity="98.69%" /Primary_Match="98.69%; Adjusted-Interval-0:0/0; Kana (Kanamycin)" /Alternative_Match="98.06%; Adjusted-Interval-0:-10/0; Kana (3 Kanamycin)" /label="Kana" promoter 11525..11797 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pNOS" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Basta" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (pNOS v2)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Kanamycin)" /Alternative_Match="98.21%; Adjusted-Interval-0:-6/0; pNOS (pNOS v1)" /Alternative_Match="97.88%; Adjusted-Interval-0:-10/0; pNOS (2 Hygromycin)" /Alternative_Match="96.88%; Adjusted-Interval-0:-10/0; pNOS (3 Kanamycin)" /Alternative_Match="96.88%; Adjusted-Interval-0:-10/0; pNOS (1 Basta)" /label="pNOS" CDS 11810..12604 /codon_start=1 /gene="nptII" /product="neomycin phosphotransferase II" /protein_id="AHE38547.1" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="nptII" terminator 12631..12898 /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (NOST)" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="2 Hygromycin" /Transferred_Similarity="93.28%" /Primary_Match="93.28%; Adjusted-Interval-0:0/0; tNOS (2 Hygromycin)" /Alternative_Match="93.28%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="93.28%; Adjusted-Interval-0:0/0; tNOS (3 Kanamycin)" /Alternative_Match="93.28%; Adjusted-Interval-0:0/0; tNOS (1 Basta)" /Alternative_Match="93.28%; Adjusted-Interval-0:0/0; tNOS (tNOS v2)" /Alternative_Match="93.28%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="93.28%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /Alternative_Match="92.89%; Adjusted-Interval-0:13/-2; tNOS (tNOS v1)" /label="tNOS" T-DNA_border 12901..13233 /modified_by="wacae" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="LB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB" /label="LB" ORIGIN 1 attcgggcac gaacccagtg gacataagcc tcgttcggtt cgtaagctgt aatgcaagta 61 gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc 121 gcagtggcgg ttttcatggc ttcttgttat gacatgtttt tttggggtac agtctatgcc 181 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag 241 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc 301 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct 361 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc 421 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg 481 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg 541 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa 601 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc 661 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc 721 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga 781 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg 841 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc 901 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat 961 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga 1021 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata 1081 atgtctagct agaaattcgt tcaagccgac gccgcttcgc cggcgttaac tcaagcgatt 1141 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta 1201 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa 1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 1441 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 1981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 2041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 2101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc 2161 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg 2221 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc 2281 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 2341 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc 2401 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc 2461 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg 2521 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca 2581 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg 2641 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt 2701 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa 2761 tgtacgtgct atccacagga aagagagctt ttcgaccttt ttcccctgct agggcaattt 2821 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc 2881 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg 2941 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg 3001 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg 3061 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg 3121 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag 3181 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc 3241 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac 3301 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct 3361 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca 3421 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg 3481 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg 3541 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac 3601 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt 3661 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg 3721 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt 3781 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg 3841 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg 3901 gtttgcgacc gtcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg 3961 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc 4021 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt 4081 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag 4141 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc 4201 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt 4261 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct 4321 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc 4381 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg 4441 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac 4501 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg 4561 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc 4621 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg 4681 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg 4741 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca 4801 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg 4861 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg 4921 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc 4981 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc 5041 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca 5101 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc 5161 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct 5221 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg 5281 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc 5341 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc 5401 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc 5461 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg 5521 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat 5581 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg 5641 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat 5701 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac 5761 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg 5821 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc 5881 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc 5941 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat 6001 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt 6061 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc 6121 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg 6181 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat 6241 ccgttattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac 6301 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctaagcttg 6361 ggcccacctc cgatccattc ggtccccaga ttagcctttt caatttcaga aagaatgcta 6421 acccacagat ggttagagag gcttacgcag cacgtctcat caagacgatc tacccgagca 6481 ataatctcca ggaaatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca 6541 ggactaactg catcaagaac acagagaaag atatatttct caagatcaga agtactattc 6601 cagtatggac gattcaaggc ttgcttcaca aaccaaggca agtaatagag attggagtct 6661 ctaaaaaggt agttcccact gaatcaaagg ccatggagtc aaagattcaa atagaggacc 6721 taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta cgactcaatg 6781 acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac tccaaaaata 6841 tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 6901 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 6961 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 7021 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 7081 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 7141 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 7201 catttcattt ggagagaaca cgggggacga gctaacagcc accatggaga agttttctca 7261 agaacagatc ggcgaaaaca ttgtgtgcag ggtcatttgt accacgggtc aaattcccat 7321 ccgagatttg tcagctgata tttcacaagt gcttaaggaa aaacgatcca taaagaaagt 7381 ttggacattt ggtagaaacc cagcctgtga ctatcattta ggaaacattt caagactgtc 7441 aaataagcat ttccaaatac tactaggaga agacggtaac cttttattga atgacatttc 7501 cactaatggg acctggttaa atgggcaaaa agtcgagaag aacagcaatc agttactgtc 7561 ccaaggtgat gaaataaccg ttggtgtagg cgtggaatca gatattttat ctctggtcat 7621 tttcataaac gacaaattta agcagtgcct ggagcagaac aaagttgatc gcggctccat 7681 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 7741 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 7801 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 7861 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 7921 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 7981 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 8041 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 8101 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 8161 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 8221 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 8281 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 8341 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg 8401 actcgagtcc ggactcagat ctggcagcgg tggaggcgat atcggcggac tcgagtccgg 8461 actcagatct ggcagcggtg gaaccttgag agaaatcgag gaattgttga ggaaaatcat 8521 tgaagacagc gtgcgcagtg tggccgagct ggaggacatt gagaagtggc tgaagaaaat 8581 ctaatcagga taactagctg agcggccatg ctagagtccg caaaaatcac cagtctctct 8641 ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag 8701 ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat 8761 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc 8821 agtgacctct gcctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag 8881 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa 8941 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag 9001 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga 9061 taaattatcg cgcgcggtgt catctatgtt actagatcga actataagct aggtagtaac 9121 tagtacctcc gatccattcg gtccccagat tagccttttc aatttcagaa agaatgctaa 9181 cccacagatg gttagagagg cttacgcagc acgtctcatc aagacgatct acccgagcaa 9241 taatctccag gaaatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag 9301 gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa gtactattcc 9361 agtatggacg attcaaggct tgcttcacaa accaaggcaa gtaatagaga ttggagtctc 9421 taaaaaggta gttcccactg aatcaaaggc catggagtca aagattcaaa tagaggacct 9481 aacagaactc gccgtaaaga ctggcgaaca gttcatacag agtctcttac gactcaatga 9541 caagaagaaa atcttcgtca acatggtgga gcacgacaca cttgtctact ccaaaaatat 9601 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc 9661 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga 9721 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga 9781 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa 9841 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt 9901 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc 9961 atttcatttg gagagaacac gggggacgag ctaacagcca ccatggggac acctcgtgat 10021 catcataacg ccaaggctag agcttgtttg ggctccggcg gactcgagtc cggactcaga 10081 tctggcagcg gtggaggcga tatcatggtg agcaagggcg aggagctgtt caccggggtg 10141 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 10201 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 10261 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc 10321 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc 10381 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 10441 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 10501 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat 10561 atcatggccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc 10621 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 10681 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc 10741 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 10801 ggcatggacg agctgtacaa gggcggactc gagtccggac tcagatctgg cagcggtgga 10861 ggcgatatcg gcggactcga gtccggactc agatctggca gcggtggagg cggagaaatc 10921 gcaaagagtc tgaaagaaat tgcaaaatcc ttgaaggaaa tagcgtggtc actgaaagaa 10981 attgccaagt ccctcaaagg gtaatcagga taactagctg agcggccatg ctagagtccg 11041 caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt 11101 gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc 11161 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct 11221 aattcctaaa accaaaatcc agtgacctct gcctagagtc aagcagatcg ttcaaacatt 11281 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa 11341 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg 11401 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa 11461 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga 11521 actagagaat taagggagtc acgttatgac ccccgccgat gacgcgggac aagccgtttt 11581 acgtttggaa ctgacagaac cgcaacgttg aaggagccac tgagccgcgg gtttctggag 11641 tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg 11701 tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc 11761 cctcggtatc caattagagt ctcatattca ctctcaactc gatcgaggca tgattgaaca 11821 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 11881 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 11941 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aagacgaggc 12001 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 12061 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 12121 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 12181 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 12241 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 12301 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgcccgacg gcgaggatct 12361 cgtcgtgacc cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 12421 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 12481 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 12541 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 12601 ctgagcggga ctctggggtt cggactctag ctagagtcaa gcagatcgtt caaacatttg 12661 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt 12721 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag 12781 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat 12841 atagcgcgca aactaggata aagtatctag aatcgcgtat gcgagtgtat gagctcgaat 12901 tcaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa 12961 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc 13021 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca 13081 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa 13141 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt 13201 gtaacgatga cagagcgttg ctgcctgtga tca //