LOCUS p35S:FHA1-eGFP-HOTag6-p35S:SOMAlongT679A-GFP-HOTag3 13473 bp DNA circular UNA 13-OCT-2023
DEFINITION .
ACCESSION urn.local...yt-gvafmxg
VERSION urn.local...yt-gvafmxg
KEYWORDS .
SOURCE
ORGANISM .
FEATURES Location/Qualifiers
Promoter 8..289
/Mismatches=0
/%_Identity=100
/Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pGGZ004"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR450)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(SpR)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR451_pFASTR_A-G
)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR449)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pGG-A-PAgro1-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pEN-L4-PAGRO1-SpCas9-
T3T-R1 )"
/X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
Pc promoter (pDEST32-GG)"
/X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
(pGADT7-GG)"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="96.81%"
/Primary_Match="96.81%; Adjusted-Interval-0:0/0; P1 (SpR)"
/label="P1"
misc_feature 290..1081
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="SpR"
/X-Transferred_Similarity="98.72%"
/X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR"
/label="SpR"
/note="Geneious type: Bacterial Selection"
rep_origin 1325..1913
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/snapgene_direction="RIGHT"
/label="ori"
regulatory complement(1949..2209)
/%_Identity=100
/Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
/annotation_group="pBR322: 7,710 -> 7,970"
/modified_by="User"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pBR322 bom"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom"
/Alternative_Match="99.29%; Adjusted-Interval-0:30/150; bom"
/label="pBR322 bom"
rep_origin 2583..2777
/note="origin of replication from Pseudomonas pVS1
plasmid"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 oriV"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV"
/label="pVS1 oriV"
regulatory complement(2843..3915)
/note="Pseudomonas pVS1 plasmid replication protein"
/product="replication protein RepA"
/gene="repA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 repA"
/Transferred_Similarity="99.91%"
/Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA"
/label="pVS1 repA"
regulatory complement(4344..4973)
/note="Pseudomonas pVS1 plasmid stability protein"
/product="plasmid stability protein StaA"
/gene="staA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 staA"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA"
/label="pVS1 staA"
T-DNA_border 6129..6358
/modified_by="wacae"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="RB"
/Transferred_Similarity="91.77%"
/Primary_Match="91.77%; Adjusted-Interval-0:0/0; RB"
/label="RB"
promoter 6370..7233
/regulatory_class="promoter"
/gene="CaMV 35S"
/note="internal BsaI recognition site removed"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCaMV 35S - Golden Gate"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Golden
Gate)"
/Alternative_Match="96.00%; Adjusted-Interval-0:505/-13;
CaMV 35S Promoter (pDe-Cas9 - Basta)"
/label="p35S"
regulatory 7238..7247
/note="vertebrate consensus sequence for strong initiation
of translation (Koza
k, 1987)"
/regulatory_class="other"
/label="Kozak sequence"
CDS 7247..7672
/translation="EKFSQEQIGENIVCRVICTTGQIPIRDLSADISQVLKEKRSIKK
VWTFGRNPACDYHLGNISRLSNKHFQILLGEDGNLLLNDISTNGTWLNGQKVEKNSNQ
LLSQGDEITVGVGVESDILSLVIFINDKFKQCLEQNKVDR"
/codon_start=1
/transl_table=1
/label="FHA"
CDS 7679..8395
/product="enhanced GFP"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
/note="mammalian codon-optimized"
/note="/vntifkey=4"
/codon_start=1
/transl_table=1
/label="EGFP"
Linker 8396..8482
/modified_by="mamee"
/label="Linker2"
CDS 8483..8584
/note="/vntifkey=21"
/modified_by="mamee"
/label="HO-Tag6"
terminator 8589..8828
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="6 35ST"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S (6 35ST)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; t35S
(t35S v1)"
/Alternative_Match="100.00%; Adjusted-Interval-0:14/0;
t35S (t35S v2)"
/Alternative_Match="100.00%; Adjusted-Interval-0:164/-6;
CaMV Poly(A) signal (pDe-Cas9 - Basta)"
/label="t35S"
terminator 8833..9100
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="NOST"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(NOST)"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="2 Hygromycin"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (2 Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(3 Kanamycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(1 Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(tNOS v2)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Kanamycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:13/-2;
tNOS (tNOS v1)"
/label="tNOS"
Linker 9105..9124
/note="/created_by=wacae"
/modified_by="mamee"
/label="Linker"
promoter 9129..9992
/regulatory_class="promoter"
/gene="CaMV 35S"
/note="internal BsaI recognition site removed"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCaMV 35S - Golden Gate"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Golden
Gate)"
/Alternative_Match="96.00%; Adjusted-Interval-0:505/-13;
CaMV 35S Promoter (pDe-Cas9 - Basta)"
/label="p35S"
regulatory 9997..10006
/note="vertebrate consensus sequence for strong initiation
of translation (Koza
k, 1987)"
/regulatory_class="other"
/label="Kozak sequence"
CDS 10051..10290
/modified_by="mamee"
/label="SOMA long"
Linker 10297..10344
/note="/vntifkey=21"
/modified_by="mamee"
/label="Linker"
CDS 10345..11061
/product="enhanced GFP"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
/note="mammalian codon-optimized"
/note="/vntifkey=4"
/codon_start=1
/transl_table=1
/label="EGFP"
Linker 11062..11151
/modified_by="mamee"
/label="Linker2"
CDS 11152..11241
/note="/vntifkey=21"
/modified_by="mamee"
/label="HO-Tag3"
terminator 11249..11488
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="6 35ST"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S (6 35ST)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; t35S
(t35S v1)"
/Alternative_Match="100.00%; Adjusted-Interval-0:14/0;
t35S (t35S v2)"
/Alternative_Match="100.00%; Adjusted-Interval-0:164/-6;
CaMV Poly(A) signal (pDe-Cas9 - Basta)"
/label="t35S"
terminator 11493..11760
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="NOST"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(NOST)"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="2 Hygromycin"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (2 Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(3 Kanamycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(1 Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(tNOS v2)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Kanamycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:13/-2;
tNOS (tNOS v1)"
/label="tNOS"
Plant_Selection 11765..13138
/Color="#006600"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Kanamycin"
/Transferred_Similarity="98.69%"
/Primary_Match="98.69%; Adjusted-Interval-0:0/0; Kana (Kanamycin)"
/Alternative_Match="98.06%; Adjusted-Interval-0:-10/0;
Kana (3 Kanamycin)"
/label="Kana"
promoter 11765..12037
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pNOS"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Basta"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(pNOS v2)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Kanamycin)"
/Alternative_Match="98.21%; Adjusted-Interval-0:-6/0; pNOS
(pNOS v1)"
/Alternative_Match="97.88%; Adjusted-Interval-0:-10/0;
pNOS (2
Hygromycin)"
/Alternative_Match="96.88%; Adjusted-Interval-0:-10/0;
pNOS (3 Kanamycin)"
/Alternative_Match="96.88%; Adjusted-Interval-0:-10/0;
pNOS (1 Basta)"
/label="pNOS"
CDS 12050..12844
/product="neomycin phosphotransferase II"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
/codon_start=1
/protein_id="AHE38547.1"
/transl_table=1
/gene="nptII"
/label="nptII"
terminator 12871..13138
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="NOST"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(NOST)"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="2 Hygromycin"
/Transferred_Similarity="93.28%"
/Primary_Match="93.28%; Adjusted-Interval-0:0/0; tNOS (2 Hygromycin)"
/Alternative_Match="93.28%; Adjusted-Interval-0:0/0; tNOS
(Basta)"
/Alternative_Match="93.28%; Adjusted-Interval-0:0/0; tNOS
(3 Kanamycin)"
/Alternative_Match="93.28%; Adjusted-Interval-0:0/0; tNOS
(1 Basta)"
/Alternative_Match="93.28%; Adjusted-Interval-0:0/0; tNOS
(tNOS v2)"
/Alternative_Match="93.28%; Adjusted-Interval-0:0/0; tNOS
(Hygromycin)"
/Alternative_Match="93.28%; Adjusted-Interval-0:0/0; tNOS
(Kanamycin)"
/Alternative_Match="92.89%; Adjusted-Interval-0:13/-2;
tNOS (tNOS v1)"
/label="tNOS"
T-DNA_border 13141..13473
/modified_by="wacae"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="LB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB"
/label="LB"
ORIGIN
1 attcgggcac gaacccagtg gacataagcc tcgttcggtt cgtaagctgt aatgcaagta
61 gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
121 gcagtggcgg ttttcatggc ttcttgttat gacatgtttt tttggggtac agtctatgcc
181 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
241 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
301 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
361 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
421 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
481 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
541 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
601 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
661 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
721 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
781 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
841 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
901 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
961 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
1021 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
1081 atgtctagct agaaattcgt tcaagccgac gccgcttcgc cggcgttaac tcaagcgatt
1141 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
1201 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
1441 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
1981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
2041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
2101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
2161 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
2221 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
2281 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
2341 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
2401 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
2461 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
2521 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
2581 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
2641 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
2701 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
2761 tgtacgtgct atccacagga aagagagctt ttcgaccttt ttcccctgct agggcaattt
2821 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
2881 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
2941 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
3001 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
3061 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
3121 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
3181 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
3241 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
3301 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
3361 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
3421 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
3481 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
3541 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
3601 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
3661 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
3721 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
3781 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
3841 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
3901 gtttgcgacc gtcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
3961 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
4021 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
4081 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
4141 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
4201 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
4261 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
4321 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
4381 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
4441 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
4501 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
4561 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
4621 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
4681 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
4741 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
4801 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
4861 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
4921 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
4981 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
5041 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
5101 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
5161 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
5221 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
5281 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
5341 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
5401 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
5461 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
5521 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
5581 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
5641 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
5701 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
5761 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
5821 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
5881 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
5941 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
6001 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
6061 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
6121 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
6181 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
6241 ccgttattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
6301 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctaagcttg
6361 ggcccacctc cgatccattc ggtccccaga ttagcctttt caatttcaga aagaatgcta
6421 acccacagat ggttagagag gcttacgcag cacgtctcat caagacgatc tacccgagca
6481 ataatctcca ggaaatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca
6541 ggactaactg catcaagaac acagagaaag atatatttct caagatcaga agtactattc
6601 cagtatggac gattcaaggc ttgcttcaca aaccaaggca agtaatagag attggagtct
6661 ctaaaaaggt agttcccact gaatcaaagg ccatggagtc aaagattcaa atagaggacc
6721 taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta cgactcaatg
6781 acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac tccaaaaata
6841 tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat
6901 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg
6961 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag
7021 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
7081 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
7141 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
7201 catttcattt ggagagaaca cgggggacga gctaacagcc accatggaga agttttctca
7261 agaacagatc ggcgaaaaca ttgtgtgcag ggtcatttgt accacgggtc aaattcccat
7321 ccgagatttg tcagctgata tttcacaagt gcttaaggaa aaacgatcca taaagaaagt
7381 ttggacattt ggtagaaacc cagcctgtga ctatcattta ggaaacattt caagactgtc
7441 aaataagcat ttccaaatac tactaggaga agacggtaac cttttattga atgacatttc
7501 cactaatggg acctggttaa atgggcaaaa agtcgagaag aacagcaatc agttactgtc
7561 ccaaggtgat gaaataaccg ttggtgtagg cgtggaatca gatattttat ctctggtcat
7621 tttcataaac gacaaattta agcagtgcct ggagcagaac aaagttgatc gcggctccat
7681 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
7741 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
7801 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
7861 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
7921 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
7981 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
8041 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
8101 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
8161 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
8221 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
8281 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
8341 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg
8401 actcgagtcc ggactcagat ctggcagcgg tggaggcgat atcggcggac tcgagtccgg
8461 actcagatct ggcagcggtg gaaccttgag agaaatcgag gaattgttga ggaaaatcat
8521 tgaagacagc gtgcgcagtg tggccgagct ggaggacatt gagaagtggc tgaagaaaat
8581 ctaatcagga taactagctg agcggccatg ctagagtccg caaaaatcac cagtctctct
8641 ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag
8701 ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
8761 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
8821 agtgacctct gcctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag
8881 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
8941 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
9001 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
9061 taaattatcg cgcgcggtgt catctatgtt actagatcga actataagct aggtagtaac
9121 tagtacctcc gatccattcg gtccccagat tagccttttc aatttcagaa agaatgctaa
9181 cccacagatg gttagagagg cttacgcagc acgtctcatc aagacgatct acccgagcaa
9241 taatctccag gaaatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag
9301 gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa gtactattcc
9361 agtatggacg attcaaggct tgcttcacaa accaaggcaa gtaatagaga ttggagtctc
9421 taaaaaggta gttcccactg aatcaaaggc catggagtca aagattcaaa tagaggacct
9481 aacagaactc gccgtaaaga ctggcgaaca gttcatacag agtctcttac gactcaatga
9541 caagaagaaa atcttcgtca acatggtgga gcacgacaca cttgtctact ccaaaaatat
9601 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc
9661 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga
9721 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga
9781 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
9841 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
9901 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
9961 atttcatttg gagagaacac gggggacgag ctaacagcca ccatggctga ccaactgact
10021 gaagagtggt cgacggtacc gcggaccggt cctggttcta agaaaatgtt ttggcggtct
10081 gcctcttggt ctgcttcacg gactgcatca caagttcctg agggtgatga gcaaagcctg
10141 aacattccgt gtgctattag ttctgggccg agtcgaagat gtccagctgc tcctttggca
10201 cctcgtgatc atcataacag caaggctaga gcttgtttgc caccattgca gcctcttgcc
10261 atttctagga ggagcttaga cgagtggcct ggctccggcg gactcgagtc cggactcaga
10321 tctggcagcg gtggaggcga tatcatggtg agcaagggcg aggagctgtt caccggggtg
10381 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
10441 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
10501 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
10561 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
10621 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
10681 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
10741 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
10801 atcatggccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
10861 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
10921 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
10981 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
11041 ggcatggacg agctgtacaa gggcggactc gagtccggac tcagatctgg cagcggtgga
11101 ggcgatatcg gcggactcga gtccggactc agatctggca gcggtggagg cggagaaatc
11161 gcaaagagtc tgaaagaaat tgcaaaatcc ttgaaggaaa tagcgtggtc actgaaagaa
11221 attgccaagt ccctcaaagg gtaatcagga taactagctg agcggccatg ctagagtccg
11281 caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt
11341 gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc
11401 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
11461 aattcctaaa accaaaatcc agtgacctct gcctagagtc aagcagatcg ttcaaacatt
11521 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
11581 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
11641 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
11701 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga
11761 actagagaat taagggagtc acgttatgac ccccgccgat gacgcgggac aagccgtttt
11821 acgtttggaa ctgacagaac cgcaacgttg aaggagccac tgagccgcgg gtttctggag
11881 tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg
11941 tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc
12001 cctcggtatc caattagagt ctcatattca ctctcaactc gatcgaggca tgattgaaca
12061 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
12121 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
12181 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aagacgaggc
12241 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
12301 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
12361 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
12421 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
12481 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
12541 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgcccgacg gcgaggatct
12601 cgtcgtgacc cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
12661 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
12721 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
12781 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
12841 ctgagcggga ctctggggtt cggactctag ctagagtcaa gcagatcgtt caaacatttg
12901 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
12961 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
13021 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
13081 atagcgcgca aactaggata aagtatctag aatcgcgtat gcgagtgtat gagctcgaat
13141 tcaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
13201 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
13261 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
13321 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
13381 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
13441 gtaacgatga cagagcgttg ctgcctgtga tca
//