LOCUS pGG-D-GUS-E(-AarI) 4500 bp DNA circular SYN 23-FEB-2023 DEFINITION . ACCESSION KF718997 VERSION KF718997.1 KEYWORDS . SOURCE Cloning vector pGGC051 (unknown) ORGANISM Cloning vector pGGC051 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4500) AUTHORS Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U. and Forner,J. TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis JOURNAL PLoS ONE 8 (12), E83043 (2013) PUBMED 24376629 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 4500) AUTHORS Forner,J. TITLE Direct Submission JOURNAL Submitted (28-SEP-2013) Centre for Organismal Studies, Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230, Heidelberg D-69221, Germany COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##. FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="D" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; D" /label="D" CDS 14..1822 /gene="gus" /codon_start=1 /transl_table=11 /product="beta-glucuronidase" /protein_id="AHE38521.1" /modified_by="mamee" /Original_Translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWW ESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTH YGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITD ENGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVAN GDVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPL RVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANS YRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGE TQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPI TCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQP IIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS QGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ" /label="GUS" misc_feature 628 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" modified_base 628 /created_by="mamee" /label misc_feature 1339 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" modified_base 1339 /created_by="mamee" /label GG complement(1826..1836) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="E" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; E" /label="E" primer complement(1856..1876) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 2356..2460 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 2461..3321 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 3492..4080 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 4444..4465 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcatca gctatggtcc gtcctgtaga aaccccaacc cgtgaaatca aaaaactcga 61 cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc gttggtggga 121 aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg atcagttcgc 181 cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag tctttatacc 241 gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc attacggcaa 301 agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc catttgaagc 361 cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt gtgtgaacaa 421 cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa acggcaagaa 481 aaagcagtct tacttccatg atttctttaa ctatgccgga atccatcgca gcgtaatgct 541 ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg tcgcgcaaga 601 ctgtaaccac gcgtctgttg actggcaagt ggtggccaat ggtgatgtca gcgttgaact 661 gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga ctttgcaagt 721 ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt gcgtcacagc 781 caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt cagtggcagt 841 gaagggcgaa cagttcctga ttaaccacaa accgttctac tttactggct ttggtcgtca 901 tgaagatgcg gacttgcgtg gcaaaggatt cgataacgtg ctgatggtgc acgaccacgc 961 attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt acgctgaaga 1021 gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg ctgctgtcgg 1081 ctttaacctc tctttaggca ttggtttcga agcgggcaac aagccgaaag aactgtacag 1141 cgaagaggca gtcaacgggg aaactcagca agcgcactta caggcgatta aagagctgat 1201 agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg aaccggatac 1261 ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg gaagcaacgc gtaaactcga 1321 cccgacgcgt ccgatcacgt gcgtcaatgt aatgttctgc gacgctcaca ccgataccat 1381 cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg tccaaagcgg 1441 cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc aggagaaact 1501 gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc tgcactcaat 1561 gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt atcaccgcgt 1621 ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg attttgcgac 1681 ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc gcgaccgcaa 1741 accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact tcggtgaaaa 1801 accgcagcag ggaggcaaac aataactgct gagaccgaat tctcgcccta tagtgagtcg 1861 tattacctgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 1921 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 1981 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt 2041 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc 2101 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga 2161 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc 2221 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata 2281 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 2341 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 2401 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 2461 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 2521 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 2581 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 2641 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 2701 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 2761 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 2821 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 2881 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 2941 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 3001 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 3061 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 3121 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggctct 3181 cgaggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 3241 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 3301 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 3361 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 3421 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 3481 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 3541 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 3601 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 3661 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 3721 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 3781 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 3841 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 3901 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 3961 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 4021 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 4081 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 4141 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 4201 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 4261 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 4321 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag 4381 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga 4441 attgtgagcg gataacaatt tcacacgagc tatttaggtg acactataga agtgaagctt //