LOCUS pDe-Cas9(VQR)-Km_ 15584 bp DNA circular UNA 21-APR-2023 DEFINITION . ACCESSION urn.local...cd-f23gs0v VERSION urn.local...cd-f23gs0v KEYWORDS . SOURCE synthetic DNA construct (unknown) ORGANISM synthetic DNA construct. FEATURES Location/Qualifiers regulatory 1179..1808 /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" regulatory 2237..3309 /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" rep_origin 3375..3569 /note="origin of replication from Pseudomonas pVS1 plasmid" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 oriV" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV" /label="pVS1 oriV" regulatory 3943..4203 /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" rep_origin complement(4239..4827) /note="origin of replication from pBR322 (also known as pMB1, pUC origin)" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ColE1" /Transferred_Similarity="99.83%" /Primary_Match="99.83%; Adjusted-Interval-0:0/0; ColE1" /label="ColE1" misc_feature complement(5071..5862) /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" Promoter complement(5863..6144) /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="96.81%" /Primary_Match="96.81%; Adjusted-Interval-0:0/0; P1 (SpR)" /label="P1" T-DNA_border 6152..6484 /modified_by="wacae" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="LB" /Transferred_Similarity="99.70%" /Primary_Match="99.70%; Adjusted-Interval-0:0/0; LB" /label="LB" regulatory 6591..6612 /note="catabolite activator protein binding site" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CAP BS" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; CAP BS" /label="CAP BS" promoter 6627..6657 /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="lacUV5" /Transferred_Similarity="93.55%" /Primary_Match="93.55%; Adjusted-Interval-0:0/0; lacUV5" /label="lacUV5" promoter 6720..7691 /created_by="lapau" /label="pPcUBI" CDS 7706..11821 /modified_by="mamee" /label="Cas9 VQR" NLS 11822..11842 /created_by="warddecaestecker" /modified_by="wacae" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on protein similarity" /Transferred_From="NLS SV40" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /label="SV40" Stop 11843..11845 /created_by="mamee" /modified_by="mamee" /label="Stop" terminator 11860..12328 /created_by="lapau" /modified_by="lapau" /label="Pea3AT" attR1 12355..12478 /label="attR1" promoter 12503..12533 /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="lacUV5" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /label="lacUV5" misc_feature 12587..13245 /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="99.85%" /Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" UTR 13268..13586 /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" CDS 13587..13892 /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.35%" /Primary_Match="99.35%; Adjusted-Interval-0:0/0; ccdB" /label="ccdB" attR2 complement(13933..14056) /label="attR2" Plant_Selection 14121..15420 /created_by="mamee" /modified_by="mamee" /label="Kana" promoter 14121..14300 /created_by="lapau" /modified_by="mamee" /label="pNOS" CDS 14334..15128 /kind="selectable marker" /note="confers resistance to neomycin and kanamycin" /product="neomycin-kanamycin phosphotransferase type II" /gene="nptII" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="neoR" /Transferred_Similarity="98.51%" /Adjusted_Transferred_Similarity="98.13%" /Primary_Match="98.51%; Adjusted-Interval-0:-9/0 (adjusted to 98.13%; Adjusted-Interval-0:0/0); neoR" /modified_by="mamee" /label="neoR" terminator 15168..15420 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; NOST" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="tNOS v1" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (tNOS v1)" /Alternative_Match="99.63%; Adjusted-Interval-0:-13/2; tNOS (Basta)" /Alternative_Match="99.63%; Adjusted-Interval-0:-13/2; tNOS (tNOS v2)" /Alternative_Match="99.63%; Adjusted-Interval-0:-13/2; tNOS (Hygromycin)" /Alternative_Match="99.63%; Adjusted-Interval-0:-13/2; tNOS (Kanamycin)" /label="tNOS" T-DNA_border 15421..15584 /created_by="mamee" /modified_by="mamee" /label="RB" ORIGIN 1 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc 61 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc 121 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg 181 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag 241 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa 301 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct 361 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag 421 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag 481 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt 541 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga 601 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat 661 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact 721 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc 781 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct 841 ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag 901 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg 961 ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct 1021 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt 1081 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg 1141 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta 1201 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac 1261 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg 1321 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc 1381 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg 1441 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa 1501 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg 1561 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca 1621 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta 1681 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag 1741 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac 1801 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg 1861 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc 1921 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc 1981 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga 2041 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg 2101 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg 2161 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca 2221 agcccgagga atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg 2281 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc 2341 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa 2401 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag 2461 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc 2521 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc 2581 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg 2641 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac 2701 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc 2761 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt 2821 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg 2881 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc 2941 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa 3001 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc 3061 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg 3121 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc 3181 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg 3241 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc 3301 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga 3361 aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac 3421 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg 3481 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa 3541 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg 3601 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct 3661 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc 3721 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc 3781 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 3841 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 3901 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt 3961 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 4021 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 4081 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 4141 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 4201 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 4261 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 4321 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 4381 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 4441 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 4501 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 4561 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 4621 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 4681 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 4741 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 4801 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 4861 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcatgatat atctcccaat 4921 ttgtgtaggg cttattatgc acgcttaaaa ataataaaag cagacttgac ctgatagttt 4981 ggctgtgagc aattatgtgc ttagtgcatc taatcgcttg agttaacgcc ggcgaagcgg 5041 cgtcggcttg aacgaatttc tagctagaca ttatttgccg actaccttgg tgatctcgcc 5101 tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc 5161 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg 5221 ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta 5281 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga 5341 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc 5401 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag 5461 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt 5521 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc 5581 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc 5641 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt 5701 caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc 5761 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc 5821 tgatagttga gtcgatactt cggcgatcac cgcttccccc atgatgttta actttgtttt 5881 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac 5941 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaacatgt 6001 cataacaaga agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg 6061 ttctggacca gttgcgtgac ggcagttacg ctacttgcat tacagcttac gaaccgaacg 6121 aggcttatgt ccactgggtt cgtgcccgaa ttgatcacag gcagcaacgc tctgtcatcg 6181 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt 6241 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc 6301 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc 6361 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg 6421 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa 6481 ttgctctagc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 6541 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 6601 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt 6661 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaattca 6721 aaaattacgg atatgaatat aggcatatcc gtatccgaat tatccgtttg acagctagca 6781 acgattgtac aattgcttct ttaaaaaagg aagaaagaaa gaaagaaaag aatcaacatc 6841 agcgttaaca aacggccccg ttacggccca aacggtcata tagagtaacg gcgttaagcg 6901 ttgaaagact cctatcgaaa tacgtaaccg caaacgtgtc atagtcagat cccctcttcc 6961 ttcaccgcct caaacacaaa aataatcttc tacagcctat atatacaacc cccccttcta 7021 tctctccttt ctcacaattc atcatctttc tttctctacc cccaatttta agaaatcctc 7081 tcttctcctc ttcattttca aggtaaatct ctctctctct ctctctctct gttattcctt 7141 gttttaatta ggtatgtatt attgctagtt tgttaatctg cttatcttat gtatgcctta 7201 tgtgaatatc tttatcttgt tcatctcatc cgtttagaag ctataaattt gttgatttga 7261 ctgtgtatct acacgtggtt atgtttatat ctaatcagat atgaatttct tcatattgtt 7321 gcgtttgtgt gtaccaatcc gaaatcgttg atttttttca tttaatcgtg tagctaattg 7381 tacgtataca tatggatcta cgtatcaatt gttcatctgt ttgtgtttgt atgtatacag 7441 atctgaaaac atcacttctc tcatctgatt gtgttgttac atacatagat atagatctgt 7501 tatatcattt tttttattaa ttgtgtatat atatatgtgc atagatctgg attacatgat 7561 tgtgattatt tacatgattt tgttatttac gtatgtatat atgtagatct ggactttttg 7621 gagttgttga cttgattgta tttgtgtgtg tatatgtgtg ttctgatctt gatatgttat 7681 gtatgtgcag cgaattcggc gcgccatgga taagaagtac tctatcggac tcgatatcgg 7741 aactaactct gtgggatggg ctgtgatcac cgatgagtac aaggtgccat ctaagaagtt 7801 caaggttctc ggaaacaccg ataggcactc tatcaagaaa aaccttatcg gtgctctcct 7861 cttcgattct ggtgaaactg ctgaggctac cagactcaag agaaccgcta gaagaaggta 7921 caccagaaga aagaacagga tctgctacct ccaagagatc ttctctaacg agatggctaa 7981 agtggatgat tcattcttcc acaggctcga agagtcattc ctcgtggaag aagataagaa 8041 gcacgagagg caccctatct tcggaaacat cgttgatgag gtggcatacc acgagaagta 8101 ccctactatc taccacctca gaaagaagct cgttgattct actgataagg ctgatctcag 8161 gctcatctac ctcgctctcg ctcacatgat caagttcaga ggacacttcc tcatcgaggg 8221 tgatctcaac cctgataact ctgatgtgga taagttgttc atccagctcg tgcagaccta 8281 caaccagctt ttcgaagaga accctatcaa cgcttcaggt gtggatgcta aggctatcct 8341 ctctgctagg ctctctaagt caagaaggct tgagaacctc attgctcagc tccctggtga 8401 gaagaagaac ggacttttcg gaaacttgat cgctctctct ctcggactca cccctaactt 8461 caagtctaac ttcgatctcg ctgaggatgc aaagctccag ctctcaaagg atacctacga 8521 tgatgatctc gataacctcc tcgctcagat cggagatcag tacgctgatt tgttcctcgc 8581 tgctaagaac ctctctgatg ctatcctcct cagtgatatc ctcagagtga acaccgagat 8641 caccaaggct ccactctcag cttctatgat caagagatac gatgagcacc accaggatct 8701 cacacttctc aaggctcttg ttagacagca gctcccagag aagtacaaag agattttctt 8761 cgatcagtct aagaacggat acgctggtta catcgatggt ggtgcatctc aagaagagtt 8821 ctacaagttc atcaagccta tcctcgagaa gatggatgga accgaggaac tcctcgtgaa 8881 gctcaataga gaggatcttc tcagaaagca gaggaccttc gataacggat ctatccctca 8941 tcagatccac ctcggagagt tgcacgctat ccttagaagg caagaggatt tctacccatt 9001 cctcaaggat aacagggaaa agattgagaa gattctcacc ttcagaatcc cttactacgt 9061 gggacctctc gctagaggaa actcaagatt cgcttggatg accagaaagt ctgaggaaac 9121 catcacccct tggaacttcg aagaggtggt ggataagggt gctagtgctc agtctttcat 9181 cgagaggatg accaacttcg ataagaacct tccaaacgag aaggtgctcc ctaagcactc 9241 tttgctctac gagtacttca ccgtgtacaa cgagttgacc aaggttaagt acgtgaccga 9301 gggaatgagg aagcctgctt ttttgtcagg tgagcaaaag aaggctatcg ttgatctctt 9361 gttcaagacc aacagaaagg tgaccgtgaa gcagctcaaa gaggattact tcaagaaaat 9421 cgagtgcttc gattcagttg agatttctgg tgttgaggat aggttcaacg catctctcgg 9481 aacctaccac gatctcctca agatcattaa ggataaggat ttcttggata acgaggaaaa 9541 cgaggatatc ttggaggata tcgttcttac cctcaccctc tttgaagata gagagatgat 9601 tgaagaaagg ctcaagacct acgctcatct cttcgatgat aaggtgatga agcagttgaa 9661 gagaagaaga tacactggtt ggggaaggct ctcaagaaag ctcattaacg gaatcaggga 9721 taagcagtct ggaaagacaa tccttgattt cctcaagtct gatggattcg ctaacagaaa 9781 cttcatgcag ctcatccacg atgattctct cacctttaaa gaggatatcc agaaggctca 9841 ggtttcagga cagggtgata gtctccatga gcatatcgct aacctcgctg gatctcctgc 9901 aatcaagaag ggaatcctcc agactgtgaa ggttgtggat gagttggtga aggtgatggg 9961 aaggcataag cctgagaaca tcgtgatcga aatggctaga gagaaccaga ccactcagaa 10021 gggacagaag aactctaggg aaaggatgaa gaggatcgag gaaggtatca aagagcttgg 10081 atctcagatc ctcaaagagc accctgttga gaacactcag ctccagaatg agaagctcta 10141 cctctactac ctccagaacg gaagggatat gtatgtggat caagagttgg atatcaacag 10201 gctctctgat tacgatgttg atcatatcgt gccacagtca ttcttgaagg atgattctat 10261 cgataacaag gtgctcacca ggtctgataa gaacaggggt aagagtgata acgtgccaag 10321 tgaagaggtt gtgaagaaaa tgaagaacta ttggaggcag ctcctcaacg ctaagctcat 10381 cactcagaga aagttcgata acttgactaa ggctgagagg ggaggactct ctgaattgga 10441 taaggcagga ttcatcaaga ggcagcttgt ggaaaccagg cagatcacta agcacgttgc 10501 acagatcctc gattctagga tgaacaccaa gtacgatgag aacgataagt tgatcaggga 10561 agtgaaggtt atcaccctca agtcaaagct cgtgtctgat ttcagaaagg atttccaatt 10621 ctacaaggtg agggaaatca acaactacca ccacgctcac gatgcttacc ttaacgctgt 10681 tgttggaacc gctctcatca agaagtatcc taagctcgag tcagagttcg tgtacggtga 10741 ttacaaggtg tacgatgtga ggaagatgat cgctaagtct gagcaagaga tcggaaaggc 10801 taccgctaag tatttcttct actctaacat catgaatttc ttcaagaccg agattaccct 10861 cgctaacggt gagatcagaa agaggccact catcgagaca aacggtgaaa caggtgagat 10921 cgtgtgggat aagggaaggg atttcgctac cgttagaaag gtgctctcta tgccacaggt 10981 gaacatcgtt aagaaaaccg aggtgcagac cggtggattc tctaaagagt ctatcctccc 11041 taagaggaac tctgataagc tcattgctag gaagaaggat tgggacccta agaaatacgg 11101 tggtttcgtg tctcctaccg tggcttactc tgttctcgtt gtggctaagg ttgagaaggg 11161 aaagagtaag aagctcaagt ctgttaagga acttctcgga atcactatca tggaaaggtc 11221 atctttcgag aagaacccaa tcgatttcct cgaggctaag ggatacaaag aggttaagaa 11281 ggatctcatc atcaagctcc caaagtactc actcttcgaa ctcgagaacg gtagaaagag 11341 gatgctcgct tctgctggtg agcttcaaaa gggaaacgag cttgctctcc catctaagta 11401 cgttaacttt ctttacctcg cttctcacta cgagaagttg aagggatctc cagaagataa 11461 cgagcagaag caacttttcg ttgagcagca caagcactac ttggatgaga tcatcgagca 11521 gatctctgag ttctctaaaa gggtgatcct cgctgatgca aacctcgata aggtgttgtc 11581 tgcttacaac aagcacagag ataagcctat cagggaacag gcagagaaca tcatccatct 11641 cttcaccctt accaacctcg gtgctcctgc tgctttcaag tacttcgata caaccatcga 11701 taggaagcag tacagatcta ccaaagaagt gctcgatgct accctcatcc atcagtctat 11761 cactggactc tacgagacta ggatcgatct ctcacagctc ggtggtgatt caagggctga 11821 tcctaagaag aagaggaagg tttgaggcgc gccgagctcc aggcctccca gctttcgtcc 11881 gtatcatcgg tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt gcccacacac 11941 cagaatccta ctaagtttga gtattatggc attggaaaag ctgttttctt ctatcatttg 12001 ttctgcttgt aatttactgt gttctttcag tttttgtttt cggacatcaa aatgcaaatg 12061 gatggataag agttaataaa tgatatggtc cttttgttca ttctcaaatt attattatct 12121 gttgttttta ctttaatggg ttgaatttaa gtaagaaagg aactaacagt gtgatattaa 12181 ggtgcaatgt tagacatata aaacagtctt tcacctctct ttggttatgt cttgaattgg 12241 tttgtttctt cacttatctg tgtaatcaag tttactatga gtctatgatc aagtaattat 12301 gcaatcaagt taagtacagt ataggcttga gctccctagg cctgttatcc ctaacaagtt 12361 tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga 12421 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggc 12481 ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat 12541 tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat 12601 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt 12661 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt 12721 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg 12781 cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg 12841 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct 12901 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc 12961 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt 13021 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa 13081 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat 13141 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct 13201 taatgaatta caacagtact gcgatgagtg gcaggcgggg cgtaatctag aggatccggc 13261 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata 13321 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta 13381 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc 13441 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa 13501 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt 13561 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga 13621 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga 13681 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtcccccgt gaactttacc 13741 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc 13801 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa 13861 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt 13921 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt 13981 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct 14041 ttcttgtaca aagtggttcg ataattccga tccagcctag gcccgggcct gaggacgcgt 14101 ccatggttaa ttaagacgtc gaaccgcaac gttgaaggag ccactcagcc gcgggtttct 14161 ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca aaagtcgcct 14221 aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac gttccataaa 14281 ttcccctcgg tatccaatta gagtctcata ttcactctca actcgatcga ggcatgattg 14341 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 14401 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 14461 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctccaagacg 14521 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 14581 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 14641 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 14701 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 14761 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 14821 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcggatgccc gacggcgagg 14881 atctcgtcgt gacccacggc gatgcctgct tgccgaatat catggtggaa aatggccgct 14941 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 15001 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 15061 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 15121 tcttctgagc gggactctgg ggttcggact ctagctagag tcaagcagat cgttcaaaca 15181 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat 15241 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta 15301 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca 15361 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc 15421 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt 15481 ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta 15541 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta cttt //