LOCUS p2GWC7 6671 bp DNA circular 12-JUL-2022
DEFINITION .
ACCESSION urn.local...1il-dsp2rnq
KEYWORDS .
SOURCE
ORGANISM .
COMMENT http://www.informaxinc.com/
This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDATE|342187383|
VNTDBDATE|457854370|
LSOWNER|
VNTNAME|p2GWC7,0|
VNTAUTHORNAME|Demo User|
VNTAUTHOREML|manka@psb.ugent.be|
VNTOAUTHORNAME|Mansour Karimi|
VNTOAUTHORTEL|+3193313850|
VNTOAUTHOREML|mansour.karimi@psb.ugent.be|
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6.05499 1.9 0.123113 1)
#282=(CLabel
(CWidget 0 (0 0) 1 2 0 0
#207# 18023728 100) (LOGPEN 0 0
8437824) 1 (LOGFONT 47
17 0 0 400 255 1 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Sac}II (233)" "@N (@S)" 5 10.4207
12.7388 0 -10 2.94183 0.694805 #281#))
(CObjectList))
#283=(CGroupWidget
(CWidget 1 (10 #17# 0) 1 2 0 0 Nil -2242 100)
(CObjectList
#284=(CScratch
(CWidget 1 (1 #17# 1) 1 2 0 0 #178#
2082602612 100) (LOGPEN 0 10 0) 8 1 5.00538
1.9 0.123113 1)
#285=(CLabel (CWidget 0 (0 0) 1 2 0 0 #207# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 1 0 0 3 2
1 34 "Arial") 1.15807 0.611111 0
"{\\i Sma}I (1349)" "@N (@S)" 5
16.5602 3.47421 0 -10 3.29663
0.694805 #284#)) (CObjectList))
#286=(CGroupWidget
(CWidget 1 (10 #18# 0) 1 2
0 0 Nil -126 100) (CObjectList
#287=(CScratch
(CWidget 1 (1 #18#
1) 1 2 0 0 #178# 36053108 100)
(LOGPEN 0 10 0) 8 1 3.75921 1.9
0.123113 1)
#288=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #207# 40990628 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 1 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Spe}I (2674)" "@N (@S)" 5 11.1112
-13.8968 0 -10 3.11923 0.694805 #287#))
(CObjectList))
#289=(CGroupWidget
(CWidget 1 (10 #19# 0) 1 2 0 0 Nil -2317 100)
(CObjectList
#290=(CScratch
(CWidget 1 (1 #19# 1) 1 2 0 0 #178#
14352800 100) (LOGPEN 0 10 0) 8 1
6.27319 1.9 0.123113 1)
#291=(CLabel
(CWidget 0 (0 0) 1 2 0 0
#207# 655377 100) (LOGPEN 0 0
8437824) 1 (LOGFONT 47
17 0 0 400 255 1 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0 "{\\i Sph}I (1)"
"@N (@S)" 5 3.78083 15.0549 0 -10 2.15833
0.694805 #290#)) (CObjectList))
#292=(CGroupWidget
(CWidget 1 (10 #22# 0) 1 2 0 0 Nil -829 100)
(CObjectList
#293=(CScratch
(CWidget 1 (1 #22# 1) 1 2 0 0 #178# 21605152
100) (LOGPEN 0 10 0) 8 1 5.00726 1.9
0.123113 1)
#294=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #207# 28920940 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 1 0 0 3 2
1 34 "Arial") 1.15807 0.611111 0
"{\\i Xma}I (1347)" "@N (@S)" 5
16.4099 5.79035 0 -10 3.29663
0.694805 #293#)) (CObjectList))
#295=(CGroupWidget
(CWidget 2 (10 #3# 0) 1 2
0 0 Nil -1767 100) (CObjectList
#296=(CScratch
(CWidget 1 (1 #3#
1) 1 2 0 0 #178# 35229876 100)
(LOGPEN 0 10 0) 8 1 5.00726 1.9
0.123113 1)
#297=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #206# 798 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Ava}I (1347)" "@N (@S)" 5 16.2499
4.63228 0 -10 3.07488 0.694805 #296#)
#298=(CScratch
(CWidget 2 (1 #3# 2) 1 2 0 0 #178# 57569264
100) (LOGPEN 0 10 0) 8 1 2.97577 1.9
0.123113 1)
#299=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #206# 29735300
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Ava}I
(3507)" "@N (@S)" 5 -4.94832 -16.213
0 -10 3.07488 0.694805 #298#))
(CObjectList))
#300=(CGroupWidget
(CWidget 2 (10 #4# 0) 1 2 0 0 Nil -200 100)
(CObjectList
#301=(CScratch
(CWidget 1 (1 #4# 1) 1 2 0 0 #178# 57571664
100) (LOGPEN 0 10 0) 8 1 4.61601 1.9
0.123113 1)
#302=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #206# 41157700 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1
34 "Arial") 1.15807 0.611111 0
"{\\i Bam}HI (1763)" "@N (@S)" 5
17.0848 -1.15807 0 -10 3.72534
0.694805 #301#) #303=(CScratch
(CWidget 2 (1 #4# 2)
1 2 0 0 #178# 57568704 100) (LOGPEN
0 10 0) 8 1 3.95483 1.9 0.123113 1)
#304=(CLabel
(CWidget 0
(0 0) 1 2 0 0 #206# 983057 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Bam}HI (2466)" "@N (@S)" 5 13.4264
-10.4226 0 -10 3.72534 0.694805 #303#))
(CObjectList))
#305=(CGroupWidget
(CWidget 2 (10 #11# 0) 1 2 0 0 Nil -2082 100)
(CObjectList
#306=(CScratch
(CWidget 1 (1 #11# 1) 1 2 0 0 #178# 28953280
100) (LOGPEN 0 10 0) 8 1 3.889 1.9 0.123113
1)
#307=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #206#
40548236 100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400
255 0 0 0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Not}I
(2536)" "@N (@S)" 5 12.4286 -11.5807
0 -10 2.9714 0.694805 #306#)
#308=(CScratch
(CWidget 2 (1 #11# 2) 1 2 0 0 #178# 57588504
100) (LOGPEN 0 10 0) 8 1 3.75263 1.9
0.123113 1)
#309=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #206# 18022856 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Not}I (2681)" "@N (@S)" 5 11.042
-15.0549 0 -10 2.9714 0.694805
#308#)) (CObjectList))) (CObjectList))
#310=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0
Nil -1377 100) (CObjectList) (CObjectList))
#311=(CGroupWidget (CWidget 12 (0 0) 1 2
0 0 Nil -437 100) (CObjectList) (CObjectList)))
(CObjectList))) (CSeqView 10 5 (CObjectList) (CObList) 1
(CObList)) (CObList) 56751380 (CStringList) 56751440 56751416
(CObList))).
FEATURES Location/Qualifiers
promoter 1..1047
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCaMV 35S - Gateway"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Gateway)"
/label="p35S"
primer_bind complement(731..752)
/%GC="45.5"
/Hairpin_Tm="41.9"
/Self_Dimer_Tm="12.2"
/Tm="59.9"
/annotation_group="598359122463180018e223f4-e38b-4f79-9447
-855d5b964c92"
/created_by="primer3"
/Sequence="CGCAATGATGGCATTTGTAGGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2GWC7"
/label="P18"
misc_feature 782
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
misc_feature 995
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
attR1 1075..1199
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR1"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1"
promoter 1224..1254
/note="Lac promoter of Escherichia coli containing 2 base
pair mutations"
/regulatory_class="promoter"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="lacUV5"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
(CmR)"
/label="lacUV5"
misc_feature 1278
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 1308..1967
/Transferred_From="cmR"
/Transferred_Similarity="99.85%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; CmR"
/label="CmR"
/note="Geneious type: Bacterial Selection"
primer_bind complement(1538..1557)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="59835911105777002f50540e-f91c-4f06-a442
-b12a08539153"
/created_by="primer3"
/Sequence="TCACCAGCTCACCGTCTTTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2GWC7"
/label="P16"
misc_feature 1892
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
UTR 1990..2308
/Mismatches=1
/%_Identity="99.68652037617555"
/Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
5'UTR (ccdB)"
/label="ccdB 5'UTR"
misc_feature 2082
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 2300
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
CDS 2309..2614
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="99.67%"
/Primary_Match="99.67%; Adjusted-Interval-0:0/0; ccdB"
/label="ccdB"
primer_bind complement(2334..2354)
/%GC="47.6"
/Hairpin_Tm="45.0"
/Self_Dimer_Tm="None"
/Tm="57.6"
/annotation_group="5983555913350200910f124d-60d5-4313-8435
-b822eb7759da"
/created_by="primer3"
/Sequence="GACGATAACGGCTCTCTCTTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2GWC7"
/label="P15"
attR2 complement(2655..2779)
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR2"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2"
CDS 2787..3506
/label="ECFP"
primer_bind complement(3082..3101)
/#_Off-target_Sites=0
/Off-target_Template="p2GWC7"
/Sequence="GCCGTCGTCCTTGAAGAAGA"
/Tm="60.0"
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P35"
terminator 3510..3735
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="t35S"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S"
/label="t35S"
primer_bind complement(3722..3741)
/%GC="55.0"
/Hairpin_Tm="44.4"
/Self_Dimer_Tm="32.8"
/Tm="60.6"
/annotation_group="5983591167813100f0d13fd9-f752-4d4d-859e
-a58ece98f412"
/created_by="primer3"
/Sequence="GCCTGCAGGTCACTGGATTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2GWC7"
/label="P17"
rep_origin 3947..4402
/label="f1_ORI"
promoter 4480..4584
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
primer_bind complement(4528..4549)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="18.4"
/Tm="60.2"
/annotation_group="5983555552599700d1e47201-199b-49e3-ba06
-358a9f942c55"
/created_by="primer3"
/Sequence="AGGGTTATTGTCTCATGAGCGG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2GWC7"
/label="P21"
misc_feature 4585..5445
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="99.77%"
/Primary_Match="99.77%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
primer_bind complement(5111..5130)
/%GC="55.0"
/Hairpin_Tm="40.4"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="59835554937698001d0d234c-2021-41fd-ba85
-1d24c14ea6da"
/created_by="primer3"
/Sequence="TACAGGCATCGTGGTGTCAC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2GWC7"
/label="P20"
rep_origin 5616..6204
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer_bind complement(5796..5815)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="5983555612044600ebbb44a2-be60-4b5c-bea1
-35794455afef"
/created_by="primer3"
/Sequence="GCGGTGCTACAGAGTTCTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2GWC7"
/label="P6"
primer_bind complement(6557..6576)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="5983590587570600ca409b60-a439-4f4c-bb0b
-529ba20912f7"
/created_by="primer3"
/Sequence="TCCGCTCACAATTCCACACA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2GWC7"
/label="P19"
ORIGIN
1 gagctctccc atatggtcga ctagagccaa gctgatctcc tttgccccgg agatcaccat
61 ggacgacttt ctctatctct acgatctagg aagaaagttc gacggagaag gtgacgatac
121 catgttcacc accgataatg agaagattag cctcttcaat ttcagaaaga atgctgaccc
181 acagatggtt agagaggcct acgcggcagg tctcatcaag acgatctacc cgagtaataa
241 tctccaggag atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac
301 taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt
361 atggacgatt caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa
421 gaaagtagtt cctactgaat caaaggccat ggagtcaaaa attcagatcg aggatctaac
481 agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa
541 gaagaaaatc ttcgtcaaca tggtggagca cgacactctc gtctactcca agaatatcaa
601 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg
661 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
721 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
781 ccctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
841 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
901 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
961 tcatttggag aggactccgg tatttttaca acaattacca caacaaaaca aacaacaaac
1021 aacattacaa tttactattc tagtcgacct gcaggcggcc gcactagtga tatcacaagt
1081 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
1141 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
1201 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
1261 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
1321 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
1381 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
1441 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
1501 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
1561 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
1621 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
1681 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
1741 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
1801 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
1861 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
1921 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg
1981 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
2041 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
2101 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
2161 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
2221 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
2281 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
2341 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
2401 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
2461 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
2521 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
2581 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
2641 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
2701 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
2761 ctttcttgta caaagtggtg atatcaatgg tgagcaaggg cgaggagctg ttcaccgggg
2821 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
2881 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
2941 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct
3001 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
3061 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
3121 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
3181 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc cacaacgtct
3241 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
3301 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
3361 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
3421 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
3481 tcggcatgga cgagctgtac aagtaaccgc ggccatgcta gagtccgcaa aaatcaccag
3541 tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc
3601 agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc
3661 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
3721 aaaatccagt gacctgcagg catgcgacgt cgggcccaat tcgccctata gtgagtcgta
3781 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
3841 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
3901 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc
3961 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
4021 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
4081 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
4141 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
4201 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
4261 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
4321 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
4381 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
4441 tttcacaccg catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
4501 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
4561 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
4621 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
4681 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
4741 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
4801 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
4861 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
4921 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
4981 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
5041 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
5101 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
5161 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
5221 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
5281 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
5341 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
5401 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
5461 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
5521 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
5581 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
5641 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
5701 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
5761 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
5821 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
5881 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
5941 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
6001 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
6061 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
6121 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
6181 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
6241 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
6301 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
6361 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
6421 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
6481 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
6541 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
6601 tatgaccatg attacgccaa gctatttagg tgacactata gaatactcaa gctatgcatc
6661 caacgcgttg g
//