LOCUS pK7WGY2 11908 bp DNA circular 12-JUL-2022
DEFINITION .
ACCESSION urn.local...1ho-dsp2fne
KEYWORDS .
SOURCE
ORGANISM .
COMMENT http://www.informaxinc.com/
This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDATE|342129009|
VNTDBDATE|457853352|
LSOWNER|
VNTNAME|pK7WGY2,0|
VNTAUTHORNAME|Demo User|
VNTAUTHOREML|manka@psb.ugent.be|
VNTOAUTHORNAME|Mansour Karimi|
VNTOAUTHORTEL|+3193313850|
VNTOAUTHOREML|mansour.karimi@psb.ugent.be|
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(LOGPEN 0 10 0) 8 1 5.47768 1.9 0.123113 1)
#287=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #221# 268786568
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 1 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Eco}RI
(1507)" "@N (@S)" 5 13.0981 6.94842
0 -10 3.50359 0.694805 #286#))
(CObjectList))
#288=(CGroupWidget
(CWidget 1 (10 #10# 0) 1 2 0 0 Nil -667 100)
(CObjectList
#289=(CScratch
(CWidget 1 (1 #10# 1) 1 2 0 0 #192# 0 100)
(LOGPEN 0 10 0) 8 1 4.2934 1.9 0.123113 1)
#290=(CLabel (CWidget 0 (0 0) 1 2 0 0 #221# 0
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 1 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Hin}dIII
(3749)" "@N (@S)" 5 13.9681 -5.79035
0 -10 3.6662 0.694805 #289#))
(CObjectList))
#291=(CGroupWidget
(CWidget 1 (10 #16# 0) 1 2 0 0 Nil -1685 100)
(CObjectList
#292=(CScratch
(CWidget 1 (1 #16# 1) 1 2 0 0 #192# 0 100)
(LOGPEN 0 10 0) 8 1 4.29446 1.9 0.123113 1)
#293=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #221# 41038020
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 1 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Sac}I
(3747)" "@N (@S)" 5 13.8867 -4.63228
0 -10 3.07488 0.694805 #292#))
(CObjectList))
#294=(CGroupWidget
(CWidget 1 (10 #20# 0) 1 2 0 0 Nil -275 100)
(CObjectList
#295=(CScratch
(CWidget 1 (1 #20# 1) 1 2 0 0 #192# 0 100)
(LOGPEN 0 10 0) 8 1 4.85702 1.9 0.123113 1)
#296=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #221# 28918004
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 1 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Spe}I
(2682)" "@N (@S)" 5 14.5017 2.31614
0 -10 3.11923 0.694805 #295#))
(CObjectList))
#297=(CGroupWidget
(CWidget 1 (10 #22# 0) 1 2 0 0 Nil -1372 100)
(CObjectList
#298=(CScratch
(CWidget 1 (1 #22# 1) 1 2 0 0 #192# 0 100)
(LOGPEN 0 10 0) 8 1 0.00528224 1.9 0.123113 1)
#299=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #221#
50392224 100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400
255 1 0 0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Xba}I
(11867)" "@N (@S)" 5 -2.76409
15.0549 0 -10 3.48881 0.694805 #298#))
(CObjectList))
#300=(CGroupWidget
(CWidget 2 (10 #17# 0) 1 2 0 0 Nil -432 100)
(CObjectList
#301=(CScratch
(CWidget 1 (1 #17# 1) 1 2 0 0 #192# 0 100)
(LOGPEN 0 10 0) 8 1 6.1443 1.9 0.123113 1)
#302=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #220# 41157700
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Sac}II
(245)" "@N (@S)" 5 4.6741 12.7388 0
-10 2.94183 0.694805 #301#) #303=(CScratch
(CWidget
2 (1 #17# 2) 1 2 0 0 #192# 0 100)
(LOGPEN 0 10 0) 8 1 0.0765924 1.9 0.123113 1)
#304=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #220# 18023048 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Sac}II (11732)" "@N (@S)" 5 -8.36574
11.5807 0 -10 3.59229 0.694805 #303#))
(CObjectList))
#305=(CGroupWidget
(CWidget 2 (10 #19# 0) 1 2 0 0 Nil -823 100)
(CObjectList
#306=(CScratch
(CWidget 1 (1 #19# 1) 1 2 0 0 #192#
0 100) (LOGPEN 0 10 0) 8 1 5.93565
1.9 0.123113 1) #307=(CLabel
(CWidget 0 (0 0) 1 2
0 0 #220# 1114129 100) (LOGPEN 0 0
8437824) 1 (LOGFONT 47
17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Sma}I (640)" "@N (@S)" 5 11.1046
9.26456 0 -10 2.98618 0.694805 #306#)
#308=(CScratch
(CWidget 2 (1 #19# 2) 1 2 0 0 #192# 0 100)
(LOGPEN 0 10 0) 8 1 0.0100363 1.9 0.123113 1)
#309=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #220# 29667940 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1
34 "Arial") 1.15807 0.611111 0
"{\\i Sma}I (11858)" "@N (@S)" 5
-2.84378 13.8968 0 -10 3.62185
0.694805 #308#)) (CObjectList))
#310=(CGroupWidget
(CWidget 2 (10 #24# 0) 1 2
0 0 Nil -980 100) (CObjectList
#311=(CScratch
(CWidget 1 (1 #24#
1) 1 2 0 0 #192# 1413562695 100)
(LOGPEN 0 10 0) 8 1 5.93671 1.9
0.123113 1)
#312=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #220# 50321712 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Xma}I (638)" "@N (@S)" 5 9.83473
10.4226 0 -10 2.98618 0.694805 #311#)
#313=(CScratch
(CWidget 2 (1 #24# 2) 1 2 0 0 #192# 0 100)
(LOGPEN 0 10 0) 8 1 0.0110927 1.9
0.123113 1) #314=(CLabel
(CWidget 0 (0 0) 1 2 0 0
#220# 393221 100) (LOGPEN 0 0
8437824) 1 (LOGFONT 47
17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Xma}I (11856)" "@N (@S)" 5 -4.99257
12.7388 0 -10 3.62185 0.694805 #313#))
(CObjectList))) (CObjectList))
#315=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -510 100)
(CObjectList) (CObjectList))
#316=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -667 100)
(CObjectList) (CObjectList))) (CObjectList)))
(CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList)
41092544 (CStringList) 67764398 14352832 (CObList))).
FEATURES Location/Qualifiers
Promoter 8..282
/Mismatches=0
/%_Identity=100
/Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
/X-Transferred_From="pGGZ004"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR450)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(SpR)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR451_pFASTR_A-G
)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR449)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pGG-A-PAgro1-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pEN-L4-PAGRO1-SpCas9-
T3T-R1 )"
/X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
Pc promoter (pDEST32-GG)"
/X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
(pGADT7-GG)"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)"
/label="P1"
misc_feature 283..1074
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="SpR"
/X-Transferred_Similarity="98.72%"
/X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR"
/label="SpR"
/note="Geneious type: Bacterial Selection"
primer_bind complement(728..747)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.1"
/annotation_group="59834566932157002ff91bca-1cfb-4e52-bc1a
-26dadf4c69ae"
/created_by="primer3"
/Sequence="ATCAAAGAGTTCCTCCGCCG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P7"
rep_origin 1320..1908
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.83%"
/Primary_Match="99.83%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer_bind complement(1500..1519)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="59834566363836001f6d15de-854e-4e6e-b300
-9951837e609a"
/created_by="primer3"
/Sequence="GCGGTGCTACAGAGTTCTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P6"
regulatory complement(1944..2204)
/%_Identity=100
/Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
/annotation_group="pBR322: 7,710 -> 7,970"
/modified_by="User"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pBR322 bom"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom"
/label="pBR322 bom"
primer_bind complement(2324..2343)
/%GC="55.0"
/Hairpin_Tm="33.5"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="598345657445080038d39f49-81f4-420d-b581
-de1b6eab9a19"
/created_by="primer3"
/Sequence="ACCTCTGACACATGCAGCTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P5"
rep_origin 2578..2772
/note="origin of replication from Pseudomonas pVS1
plasmid"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 oriV"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV"
/label="pVS1 oriV"
regulatory complement(2838..3911)
/note="Pseudomonas pVS1 plasmid replication protein"
/product="replication protein RepA"
/gene="repA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 repA"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 repA"
/label="pVS1 repA"
primer_bind complement(3126..3143)
/%GC="61.1"
/Hairpin_Tm="None"
/Self_Dimer_Tm="0.7"
/Tm="60.0"
/annotation_group="598345651060530074f4c3e0-1ecf-46e5-9f8f
-252962e4c5ca"
/created_by="primer3"
/Sequence="AAGAACCCGGACGTGCTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P4"
primer_bind complement(3929..3947)
/%GC="63.2"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="61.0"
/annotation_group="598345645307340074060a7f-25f4-4f48-b9ab
-1600695197b9"
/created_by="primer3"
/Sequence="CAATGGCACTGGAACCCCC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P3"
regulatory complement(4340..4969)
/note="Pseudomonas pVS1 plasmid stability protein"
/product="plasmid stability protein StaA"
/gene="staA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 staA"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA"
/label="pVS1 staA"
primer_bind complement(4725..4744)
/%GC="55.0"
/Hairpin_Tm="42.2"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="5983456393552700a420f618-e3b9-4ceb-a4fe
-0fb05fbe8f04"
/created_by="primer3"
/Sequence="CGCGACTTCGTAGTGATCGA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P2"
primer_bind complement(5518..5536)
/%GC="63.2"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="61.3"
/annotation_group="59834563367533007668152c-c617-4ce7-b148
-4af2613a73d5"
/created_by="primer3"
/Sequence="CCCGAGGCGTGAAGTTTGG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P1"
T-DNA_border 6185..6385
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="RB"
/Transferred_Similarity="99.50%"
/Primary_Match="99.50%; Adjusted-Interval-0:0/0; RB"
/label="RB"
primer_bind complement(6345..6364)
/%GC="50.0"
/Hairpin_Tm="36.2"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="59834570721406009e3a251e-4811-4122-a23b
-26a51c8b9839"
/created_by="primer3"
/Sequence="AGATTGTCGTTTCCCGCCTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P14"
promoter 6387..7433
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCaMV 35S - Gateway"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Gateway)"
/label="p35S"
primer_bind complement(7117..7138)
/%GC="45.5"
/Hairpin_Tm="41.9"
/Self_Dimer_Tm="12.2"
/Tm="59.9"
/annotation_group="59834573059068000a92377d-9804-4fa7-8af3
-cc60ab625d35"
/created_by="primer3"
/Sequence="CGCAATGATGGCATTTGTAGGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P18"
CDS 7455..8171
/label="EYFP"
primer_bind complement(7954..7973)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.2"
/annotation_group="59834574820495002e82027d-14ee-4252-a207
-2f424eb5d05b"
/created_by="primer3"
/Sequence="CTCGATGTTGTGGCGGATCT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P33"
attR1 8178..8302
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR1"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1"
promoter 8327..8357
/note="Lac promoter of Escherichia coli containing 2 base
pair mutations"
/regulatory_class="promoter"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="lacUV5"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
(CmR)"
/label="lacUV5"
misc_feature 8411..9070
/Transferred_From="cmR"
/Transferred_Similarity="99.85%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; CmR"
/label="CmR"
/note="Geneious type: Bacterial Selection"
primer_bind complement(8641..8660)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="59834571868604008b7e992f-29ce-41ad-95ce
-dc21ed142616"
/created_by="primer3"
/Sequence="TCACCAGCTCACCGTCTTTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P16"
UTR 9093..9411
/Mismatches=1
/%_Identity="99.68652037617555"
/Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
5'UTR (ccdB)"
/label="ccdB 5'UTR"
CDS 9412..9717
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="99.67%"
/Primary_Match="99.67%; Adjusted-Interval-0:0/0; ccdB"
/label="ccdB"
primer_bind complement(9437..9457)
/%GC="47.6"
/Hairpin_Tm="45.0"
/Self_Dimer_Tm="None"
/Tm="57.6"
/annotation_group="598345713014220078b1ccca-f3db-4ffd-a3a6
-fa3c6436fa29"
/created_by="primer3"
/Sequence="GACGATAACGGCTCTCTCTTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P15"
attR2 complement(9758..9882)
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR2"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2"
terminator 9892..10117
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="t35S"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S"
/label="t35S"
primer_bind complement(10104..10123)
/%GC="55.0"
/Hairpin_Tm="44.4"
/Self_Dimer_Tm="32.8"
/Tm="60.6"
/annotation_group="59834572481156006e3e99fa-ea63-4185-b21f
-10e2fcd99870"
/created_by="primer3"
/Sequence="GCCTGCAGGTCACTGGATTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P17"
promoter 10182..10454
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pNOS"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Basta"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Kanamycin)"
/label="pNOS"
Plant_Selection 10182..11555
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Kanamycin"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (Kanamycin)"
/label="Kana"
CDS 10467..11261
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="NptII"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; NptII"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
NptII (Kanamycin)"
/label="NptII"
primer_bind complement(10506..10525)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.5"
/annotation_group="5983457424095200c37a76e0-5a29-4ab0-9f33
-bbb0ceb1de19"
/created_by="primer3"
/Sequence="AATAGCCTCTCCACCCAAGC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P25"
primer_bind complement(11119..11138)
/%GC="50.0"
/Hairpin_Tm="46.1"
/Self_Dimer_Tm="None"
/Tm="59.2"
/annotation_group="5983457366048200e30d3746-81bf-42c8-a5a9
-eaf4ca80fd74"
/created_by="primer3"
/Sequence="AGCCAACGCTATGTCCTGAT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P24"
terminator 11288..11555
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="NOST"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; NOST"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="tNOS"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Kanamycin)"
/label="tNOS"
T-DNA_border 11576..11908
/modified_by="wacae"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="LB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB"
/label="LB"
primer_bind complement(11820..11839)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="59834567813044005e99910b-dd0a-455a-bf90
-14f1f5809ec7"
/created_by="primer3"
/Sequence="TCAAACCCGGCAGCTTAGTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7WGY2"
/label="P8"
ORIGIN
1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
301 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
361 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
421 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
481 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
541 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
601 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
661 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
721 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
781 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
841 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
901 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
961 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
1021 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
1081 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
1141 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
1201 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct
1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
1381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
1441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
1621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
2041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
2101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
2161 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
2221 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
2281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
2341 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
2401 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
2461 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
2521 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
2581 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
2641 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
2701 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
2761 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
2821 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
2881 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
2941 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
3001 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
3061 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
3121 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
3181 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
3241 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
3301 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
3361 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
3421 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
3481 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
3541 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
3601 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
3661 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
3721 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
3781 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
3841 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
3901 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
3961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
4021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
4081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
4141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
4201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
4261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
4321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
4381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
4441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
4501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
4561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
4621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
4681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
4741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
4801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
4861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
4921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
4981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
5041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
5101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
5161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
5221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
5281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
5341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
5401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
5461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
5521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
5581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
5641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
5701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
5761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
5821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
5881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
5941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
6001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
6061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
6121 gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa
6181 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
6241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
6301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
6361 atctgatcca agctcaagct aagcttgagc tctcccatat ggtcgactag agccaagctg
6421 atctcctttg ccccggagat caccatggac gactttctct atctctacga tctaggaaga
6481 aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa gattagcctc
6541 ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc ggcaggtctc
6601 atcaagacga tctacccgag taataatctc caggagatca aataccttcc caagaaggtt
6661 aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt
6721 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg
6781 caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa ggccatggag
6841 tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga acagttcata
6901 cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac
6961 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
7021 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
7081 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
7141 aaaggaaagg ctatcgttca agatgcccct gccgacagtg gtcccaaaga tggaccccca
7201 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
7261 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
7321 ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa
7381 ttaccacaac aaaacaaaca acaaacaaca ttacaattta ctattctagt cgacctgcag
7441 gcggccgcac tagtatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
7501 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
7561 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
7621 tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc
7681 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
7741 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
7801 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
7861 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
7921 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
7981 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
8041 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
8101 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
8161 agctgtacac ggatatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
8221 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
8281 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
8341 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
8401 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
8461 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
8521 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
8581 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
8641 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
8701 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
8761 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
8821 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
8881 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
8941 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
9001 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
9061 cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
9121 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
9181 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
9241 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
9301 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
9361 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
9421 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
9481 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
9541 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
9601 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
9661 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
9721 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
9781 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
9841 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatatccc gcggccatgc
9901 tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca
9961 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat
10021 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
10081 aaaatttcta attcctaaaa ccaaaatcca gtgacctgca ggcatgcgac gtcgggccct
10141 ctagaggatc cccgggtacc gcgaattatc gatcatgagc ggagaattaa gggagtcacg
10201 ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc
10261 aacgttgaag gagccactga gccgcgggtt tctggagttt aatgagctaa gcacatacgt
10321 cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt
10381 cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc
10441 atattcactc tcaactcgat cgaggcatga ttgaacaaga tggattgcac gcaggttctc
10501 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
10561 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
10621 acctgtccgg tgccctgaat gaactccaag acgaggcagc gcggctatcg tggctggcca
10681 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
10741 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
10801 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
10861 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
10921 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
10981 ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacccac ggcgatgcct
11041 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
11101 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
11161 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
11221 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgg
11281 actctagcta gagtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga
11341 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
11401 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
11461 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
11521 tatcgcgcgc ggtgtcatct atgttactag atcgaccggc atgcaagctg ataattcaat
11581 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
11641 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
11701 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
11761 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
11821 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
11881 gatgacagag cgttgctgcc tgtgatca
//