LOCUS pK7WGC2 11908 bp DNA circular 12-JUL-2022 DEFINITION . ACCESSION urn.local...1ec-dsp132u KEYWORDS . SOURCE ORGANISM . COMMENT http://www.informaxinc.com/ This file is created by Vector NTI http://www.invitrogen.com/ ORIGDB|GenBank VNTDATE|342173526| VNTDBDATE|457853266| LSOWNER| VNTNAME|pK7WGC2,0| VNTAUTHORNAME|Demo User| VNTAUTHOREML|manka@psb.ugent.be| VNTOAUTHORNAME|Mansour Karimi| VNTOAUTHORTEL|+3193313850| VNTOAUTHOREML|mansour.karimi@psb.ugent.be| Vector_NTI_Display_Data_(Do_Not_Edit!) (SXF (CGexDoc "pK7WGC2,0" 0 11876 (CDBMol 0 0 1 1 1 0 0 1 1 "" "" 0 0 0 0 (CObList) (CObList) (CObList #0=(CFragment 3 1 0 42487116 0 "" 0 "" "" "" "" "" "" "p2CGW7" "" 0 0 1 2681 1 2681 6423 2437 0 "" "" "" "" "" "" (CObList) (CTerm 1 0 0 "ApaI" "" 1 0 0 0 0 0 0 0 0 0) (CTerm 1 1 0 "SpeI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) #1=(CFragment 3 1 0 1081671680 0 "" 0 "" "" "" "" "" "" "pK7YWG2,0" "" 0 0 1 9195 2682 11876 2686 11880 0 "" "" "" "" "" "" (CObList) (CTerm 1 0 0 "SpeI" "" 1 0 0 0 0 0 0 0 0 0) (CTerm 1 1 0 "ApaI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") (CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 5 40 50 0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList "AatII" "ApaI" "AseI" "AvaI" "BamHI" "ClaI" "EcoRI" "EcoRV" "HindIII" "NcoI" "NdeI" "NotI" "NruI" "PstI" "SacI" "SacII" "SalI" "SmaI" "SpeI" "SphI" "XbaI" "XhoI" "XmaI" "BglII") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) (CMolPar 0 0 0 0 0 1 11876 0 0 0 0 0 0 0 0) (CStringList) (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList #2=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 7 0 0 "") #3=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 1 0 0 "") #4=(CRSite (CStringList) "AseI" "attaat" 3 0 0 0 "") #5=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 8 638 0 3515 0 3938 0 4568 0 5699 0 6179 0 9923 0 11856 0 0 "") #6=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 1054 0 1757 0 11861 0 0 "") #7=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 7003 0 0 "") #8=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1507 0 0 "") #9=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 3 250 0 1961 0 2865 0 0 "") #10=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 3749 0 0 "") #11=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 1206 0 3297 0 3687 0 0 "") #12=(CRSite (CStringList) "NdeI" "catatg" 3 0 3 3735 0 5468 0 7957 0 0 "") #13=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 5 1827 0 2689 0 4799 0 6331 0 7621 0 0 "") #14=(CRSite (CStringList) "NruI" "tcgcga" 4 0 0 0 "") #15=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 19 0 389 0 2700 0 0 "") #16=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 3747 0 0 "") #17=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 245 0 11732 0 0 "") #18=(CRSite (CStringList) "SalI" "gtcgac" 2 0 3 379 0 2702 0 3728 0 0 "") #19=(CRSite (CStringList) "SmaI" "cccggg" 4 0 2 640 0 11858 0 0 "") #20=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 2682 0 0 "") #21=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 13 0 3920 0 10456 0 0 "") #22=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 11867 0 0 "") #23=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "") #24=(CRSite (CStringList) "XmaI" "cccggg" 2 0 2 638 0 11856 0 0 "") #25=(CRSite (CStringList) "BglII" "agatct" 2 0 0 0 "")) (CObList #26=(CFSignal (CObList) "ECFP" 4 0 1 1964 2680 0 (CStringList) (CStringList) 1 1 1 1 "complement(1964..2680)") #27=(CFSignal (CObList) "attR1" 86 0 1 1833 1957 0 (CStringList) (CStringList) 1 1 1 1 "complement(1833..1957)") #28=(CFSignal (CObList) "ccdB" 21 0 1 378 1832 0 (CStringList) (CStringList) 1 1 1 1 "complement(378..1832)") #29=(CFSignal (CObList) "attR2" 86 0 0 253 377 0 (CStringList) (CStringList) 1 1 1 1 "253..377") #30=(CFSignal (CObList) "T35S" 43 0 0 18 243 0 (CStringList) (CStringList) 1 1 1 1 "18..243") #31=(CFSignal (CObList) "p35S" 29 0 1 2702 3729 0 (CStringList) (CStringList) 1 1 1 1 "complement(2702..3729)") #32=(CFSignal (CObList) "RB" 87 0 0 3749 3948 0 (CStringList) (CStringList) 1 1 1 1 "3749..3948") #33=(CFSignal (CObList) "Sm/SpR" 22 0 1 8852 10101 0 (CStringList) (CStringList) 1 1 1 1 "complement(8852..10101)") #34=(CFSignal (CObList) "LB" 87 0 0 10107 10439 0 (CStringList) (CStringList) 1 1 1 1 "10107..10439") #35=(CFSignal (CObList) "Kan" 22 0 1 10443 11839 0 (CStringList) (CStringList) 1 1 1 1 "complement(10443..11839)")) (CObList) (CObList) (CObList) (CObList) (CObList) (CObList) (CTextView 0 #36=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) (CObjectList #37=(CRefLinePar (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pK7WGC2,0" 2) 5 "" 0 4) #38=(CFolderPar (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) (CObjectList #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "DNA 'pK7WGC2,0'" 1) #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Currently local object. Original author: Mansour Karimi" 1) #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Created: 06/24/04 08:12" 1) #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Last Modified: 09/19/07 05:27" 1) #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "length: 11876 bp" 1) #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "storage type: Constructed" 1) #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "form: Circular" 1))) "General Description") #46=(CFolderPar (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Original Source Database: GenBank" 1) #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Modification Date in the Original DB: 24-JUN-2004" 1))) "Standard Fields") #49=(CFolderPar (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) (CObjectList #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Mansour Karimi" 1) #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "TEL: +3193313850 " 1) #52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "EMAIL: mansour.karimi@psb.ugen t.be" 1))) "Original Author") #53=(CRefLinePar (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 0 0) #54=(CFolderPar (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) "Annotations") #55=(CFolderPar (CGroupPar (CParagraph 10 (0 0) 1 1 0 0 178) (CObjectList #56=(CFolderPar (CGroupPar (CParagraph 0 (5 #0# 0) 1 2 1 0 195) (CObjectList #57=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "parent position: from 6423 to 2437" 1) #58=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "original length: 2681" 1) #59=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "molecule position: from 1 to 2681" 1) #60=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #61=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " ApaI site #1" 1))) "Left Terminus") #62=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #63=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " SpeI site #1" 1))) "Right Terminus"))) "#1: FRAGMENT of p2CGW7 ") #64=(CFolderPar (CGroupPar (CParagraph 0 (5 #1# 0) 1 2 1 0 195) (CObjectList #65=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "parent position: from 2686 to 11880" 1) #66=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "original length: 9195" 1) #67=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "molecule position: from 2682 to 11876" 1) #68=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #69=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " SpeI site #1" 1))) "Left Terminus") #70=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #71=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " ApaI site #1" 1))) "Right Terminus"))) "#2: FRAGMENT of pK7YWG2,0 "))) "Component Fragments") #72=(CFolderPar (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) (CObjectList #73=(CFolderPar (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) (CObjectList #74=(CFolderPar (CGroupPar (CParagraph 1964 (3 #26# 0) 1 2 2 0 327) (CObjectList #75=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 1964 End: 2680 (Complementary)" 1) #76=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #77=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(1964..2680)" 1))) "ECFP"))) "CDS (1 total)") #78=(CFolderPar (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) (CObjectList #79=(CFolderPar (CGroupPar (CParagraph 378 (3 #28# 0) 1 2 2 0 194) (CObjectList #80=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 378 End: 1832 (Complementary)" 1) #81=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #82=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(378..1832)" 1))) "ccdB"))) "Misc. Feature (1 total)") #83=(CFolderPar (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) (CObjectList #84=(CFolderPar (CGroupPar (CParagraph 8852 (3 #33# 0) 1 2 2 0 194) (CObjectList #85=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 8852 End: 10101 (Complementary)" 1) #86=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #87=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(8852..10101)" 1))) "Sm/SpR") #88=(CFolderPar (CGroupPar (CParagraph 10443 (3 #35# 0) 1 2 2 0 194) (CObjectList #89=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 10443 End: 11839 (Complementary)" 1) #90=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #91=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(10443..11839)" 1))) "Kan"))) "Misc. Marker (2 total)") #92=(CFolderPar (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) (CObjectList #93=(CFolderPar (CGroupPar (CParagraph 2702 (3 #31# 0) 1 2 2 0 194) (CObjectList #94=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 2702 End: 3729 (Complementary)" 1) #95=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "35S promoter plus a part of omega leader" 1) #96=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #97=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(2702..3729)" 1))) "p35S"))) "Promoter Eukaryotic (1 total)") #98=(CFolderPar (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) (CObjectList #99=(CFolderPar (CGroupPar (CParagraph 18 (3 #30# 0) 1 2 2 0 194) (CObjectList #100=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 18 End: 243 " 1) #101=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #102=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 18..243" 1))) "T35S"))) "Terminator (1 total)") #103=(CFolderPar (CGroupPar (CParagraph 86 (7 86 0) 1 1 1 0 178) (CObjectList #104=(CFolderPar (CGroupPar (CParagraph 253 (3 #29# 0) 1 2 2 0 194) (CObjectList #105=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 253 End: 377 " 1) #106=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #107=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 253..377" 1))) "attR2") #108=(CFolderPar (CGroupPar (CParagraph 1833 (3 #27# 0) 1 2 2 0 194) (CObjectList #109=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 1833 End: 1957 (Complementary)" 1) #110=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #111=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(1833..1957)" 1))) "attR1"))) "Misc. Recombination (2 total)") #112=(CFolderPar (CGroupPar (CParagraph 87 (7 87 0) 1 1 1 0 178) (CObjectList #113=(CFolderPar (CGroupPar (CParagraph 3749 (3 #32# 0) 1 2 2 0 194) (CObjectList #114=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 3749 End: 3948" 1) #115=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #116=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 3749..3948" 1))) "RB") #117=(CFolderPar (CGroupPar (CParagraph 10107 (3 #34# 0) 1 2 2 0 194) (CObjectList #118=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 10107 End: 10439" 1) #119=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #120=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 10107..10439" 1))) "LB"))) "Misc. Signal (2 total)"))) "Feature Map") #121=(CFolderPar (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) (CObjectList #122=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42794072 (8 0) 1 1 1 0 178) (CObjectList #123=(CGroupPar (CParagraph 0 (10 #2# 0) 1 2 2 0 180) (CObjectList #124=(CLinePar (CParagraph 0 (1 #2# 1) 1 2 2 0 191) " N1: 7 " 1))))) "AatII: 1 site") 5 1 "GACGTC" "CTGCAG") #125=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42794040 (8 0) 1 1 1 0 178) (CObjectList #126=(CGroupPar (CParagraph 0 (10 #3# 0) 1 2 2 0 180) (CObjectList #127=(CLinePar (CParagraph 0 (1 #3# 1) 1 2 2 0 191) " N1: 1 " 1))))) "ApaI: 1 site") 5 1 "GGGCCC" "CCCGGG") #128=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42794008 (8 0) 1 1 1 0 178) (CObjectList)) "AseI: 0 sites") 2 4 "ATTAAT" "TAATTA") #129=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42793976 (8 0) 1 1 1 0 178) (CObjectList)) "AvaI: 8 sites") 1 5 "CYCGRG" "GRGCYC") #130=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42793944 (8 0) 1 1 1 0 178) (CObjectList)) "BamHI: 3 sites") 1 5 "GGATCC" "CCTAGG") #131=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42911920 (8 0) 1 1 1 0 178) (CObjectList)) "BglII: 0 sites") 1 5 "AGATCT" "TCTAGA") #132=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42793912 (8 0) 1 1 1 0 178) (CObjectList #133=(CGroupPar (CParagraph 0 (10 #7# 0) 1 2 2 0 180) (CObjectList #134=(CLinePar (CParagraph 0 (1 #7# 1) 1 2 2 0 191) " N1: 7003 " 1))))) "ClaI: 1 site") 2 4 "ATCGAT" "TAGCTA") #135=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42793880 (8 0) 1 1 1 0 178) (CObjectList #136=(CGroupPar (CParagraph 0 (10 #8# 0) 1 2 2 0 180) (CObjectList #137=(CLinePar (CParagraph 0 (1 #8# 1) 1 2 2 0 191) " N1: 1507 " 1))))) "EcoRI: 1 site") 1 5 "GAATTC" "CTTAAG") #138=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42793848 (8 0) 1 1 1 0 178) (CObjectList)) "EcoRV: 3 sites") 3 3 "GATATC" "CTATAG") #139=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42793816 (8 0) 1 1 1 0 178) (CObjectList #140=(CGroupPar (CParagraph 0 (10 #10# 0) 1 2 2 0 180) (CObjectList #141=(CLinePar (CParagraph 0 (1 #10# 1) 1 2 2 0 191) " N1: 3749 " 1))))) "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") #142=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42793648 (8 0) 1 1 1 0 178) (CObjectList)) "NcoI: 3 sites") 1 5 "CCATGG" "GGTACC") #143=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42794504 (8 0) 1 1 1 0 178) (CObjectList)) "NdeI: 3 sites") 2 4 "CATATG" "GTATAC") #144=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42794472 (8 0) 1 1 1 0 178) (CObjectList)) "NotI: 5 sites") 2 6 "GCGGCCGC" "CGCCGGCG") #145=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42794304 (8 0) 1 1 1 0 178) (CObjectList)) "NruI: 0 sites") 3 3 "TCGCGA" "AGCGCT") #146=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42794272 (8 0) 1 1 1 0 178) (CObjectList)) "PstI: 3 sites") 5 1 "CTGCAG" "GACGTC") #147=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42794104 (8 0) 1 1 1 0 178) (CObjectList #148=(CGroupPar (CParagraph 0 (10 #16# 0) 1 2 2 0 180) (CObjectList #149=(CLinePar (CParagraph 0 (1 #16# 1) 1 2 2 0 191) " N1: 3747 " 1))))) "SacI: 1 site") 5 1 "GAGCTC" "CTCGAG") #150=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42911664 (8 0) 1 1 1 0 178) (CObjectList #151=(CGroupPar (CParagraph 0 (10 #17# 0) 1 2 2 0 180) (CObjectList #152=(CLinePar (CParagraph 0 (1 #17# 1) 1 2 2 0 191) " N1: 245 " 1) #153=(CLinePar (CParagraph 0 (1 #17# 2) 1 2 2 0 191) " N2: 11732 " 1))))) "SacII: 2 sites") 4 2 "CCGCGG" "GGCGCC") #154=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42911696 (8 0) 1 1 1 0 178) (CObjectList)) "SalI: 3 sites") 1 5 "GTCGAC" "CAGCTG") #155=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42911728 (8 0) 1 1 1 0 178) (CObjectList #156=(CGroupPar (CParagraph 0 (10 #19# 0) 1 2 2 0 180) (CObjectList #157=(CLinePar (CParagraph 0 (1 #19# 1) 1 2 2 0 191) " N1: 640 " 1) #158=(CLinePar (CParagraph 0 (1 #19# 2) 1 2 2 0 191) " N2: 11858 " 1))))) "SmaI: 2 sites") 3 3 "CCCGGG" "GGGCCC") #159=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42911760 (8 0) 1 1 1 0 178) (CObjectList #160=(CGroupPar (CParagraph 0 (10 #20# 0) 1 2 2 0 180) (CObjectList #161=(CLinePar (CParagraph 0 (1 #20# 1) 1 2 2 0 191) " N1: 2682 " 1))))) "SpeI: 1 site") 1 5 "ACTAGT" "TGATCA") #162=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42911792 (8 0) 1 1 1 0 178) (CObjectList)) "SphI: 3 sites") 5 1 "GCATGC" "CGTACG") #163=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42911824 (8 0) 1 1 1 0 178) (CObjectList #164=(CGroupPar (CParagraph 0 (10 #22# 0) 1 2 2 0 180) (CObjectList #165=(CLinePar (CParagraph 0 (1 #22# 1) 1 2 2 0 191) " N1: 11867 " 1))))) "XbaI: 1 site") 1 5 "TCTAGA" "AGATCT") #166=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42911856 (8 0) 1 1 1 0 178) (CObjectList)) "XhoI: 0 sites") 1 5 "CTCGAG" "GAGCTC") #167=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 42911888 (8 0) 1 1 1 0 178) (CObjectList #168=(CGroupPar (CParagraph 0 (10 #24# 0) 1 2 2 0 180) (CObjectList #169=(CLinePar (CParagraph 0 (1 #24# 1) 1 2 2 0 191) " 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(CStringList) 1096810496 327685 (CObList))). FEATURES Location/Qualifiers Promoter 8..282 /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /label="P1" misc_feature 283..1074 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" primer_bind complement(728..747) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.1" /annotation_group="5983376076770200226447f9-81e9-4c77-8ff7 -9fb8ee7fea90" /created_by="primer3" /Sequence="ATCAAAGAGTTCCTCCGCCG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P7" rep_origin 1320..1908 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.83%" /Primary_Match="99.83%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer_bind complement(1500..1519) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="58.9" /annotation_group="5983376018488600094a8662-c324-4714-9d2b -6dc7b73360d1" /created_by="primer3" /Sequence="GCGGTGCTACAGAGTTCTTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P6" regulatory complement(1944..2204) /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /label="pBR322 bom" primer_bind complement(2324..2343) /%GC="55.0" /Hairpin_Tm="33.5" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="5983375959583900af1a7738-063b-4c20-9c88 -604e175d9e79" /created_by="primer3" /Sequence="ACCTCTGACACATGCAGCTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P5" rep_origin 2578..2772 /note="origin of replication from Pseudomonas pVS1 plasmid" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 oriV" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV" /label="pVS1 oriV" regulatory complement(2838..3911) /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" primer_bind complement(3126..3143) /%GC="61.1" /Hairpin_Tm="None" /Self_Dimer_Tm="0.7" /Tm="60.0" /annotation_group="5983375901596200395045dc-ebaa-4b11-8403 -1dc7c972c579" /created_by="primer3" /Sequence="AAGAACCCGGACGTGCTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P4" primer_bind complement(3929..3947) /%GC="63.2" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="61.0" /annotation_group="5983375842859800bff7c435-e048-43c4-bb22 -7ba8a090356b" /created_by="primer3" /Sequence="CAATGGCACTGGAACCCCC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P3" regulatory complement(4340..4969) /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" primer_bind complement(4725..4744) /%GC="55.0" /Hairpin_Tm="42.2" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="5983375782833100c2ba5c0e-f208-4721-9acc -dbc84cc44002" /created_by="primer3" /Sequence="CGCGACTTCGTAGTGATCGA" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P2" primer_bind complement(5518..5536) /%GC="63.2" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="61.3" /annotation_group="598337571406920024a4f169-df53-4e95-84f1 -304f46b476ce" /created_by="primer3" /Sequence="CCCGAGGCGTGAAGTTTGG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P1" T-DNA_border 6185..6385 /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="RB" /Transferred_Similarity="99.50%" /Primary_Match="99.50%; Adjusted-Interval-0:0/0; RB" /label="RB" primer_bind complement(6345..6364) /%GC="50.0" /Hairpin_Tm="36.2" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="5983376432258700bf04f6bc-51f3-4b8f-8bb0 -c84099eca607" /created_by="primer3" /Sequence="AGATTGTCGTTTCCCGCCTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P14" promoter 6387..7433 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCaMV 35S - Gateway" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Gateway)" /label="p35S" primer_bind complement(7117..7138) /%GC="45.5" /Hairpin_Tm="41.9" /Self_Dimer_Tm="12.2" /Tm="59.9" /annotation_group="598337666244470088f69fe7-0460-44c0-b35f -965904538c73" /created_by="primer3" /Sequence="CGCAATGATGGCATTTGTAGGT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P18" CDS 7455..8171 /label="ECFP" primer_bind complement(7954..7973) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.2" /annotation_group="598337683746970060056c7b-bc7e-4a34-b43e -88af31e4f886" /created_by="primer3" /Sequence="CTCGATGTTGTGGCGGATCT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P33" attR1 8178..8302 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR1" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1" promoter 8327..8357 /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="lacUV5" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (CmR)" /label="lacUV5" misc_feature 8411..9070 /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" primer_bind complement(8641..8660) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="5983376547926500be734a42-4152-4679-86c6 -12750a7ee5c0" /created_by="primer3" /Sequence="TCACCAGCTCACCGTCTTTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P16" UTR 9093..9411 /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" CDS 9412..9717 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.67%" /Primary_Match="99.67%; Adjusted-Interval-0:0/0; ccdB" /label="ccdB" primer_bind complement(9437..9457) /%GC="47.6" /Hairpin_Tm="45.0" /Self_Dimer_Tm="None" /Tm="57.6" /annotation_group="5983376489378300e2d09e63-0546-478e-91b9 -3f2959eedf66" /created_by="primer3" /Sequence="GACGATAACGGCTCTCTCTTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P15" attR2 complement(9758..9882) /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2" terminator 9892..10117 /created_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="t35S" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S" /label="t35S" primer_bind complement(10104..10123) /%GC="55.0" /Hairpin_Tm="44.4" /Self_Dimer_Tm="32.8" /Tm="60.6" /annotation_group="59833766055555008d4d99ad-aba0-4e64-9678 -91a366b6a8c1" /created_by="primer3" /Sequence="GCCTGCAGGTCACTGGATTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P17" promoter 10182..10454 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pNOS" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Basta" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Kanamycin)" /label="pNOS" Plant_Selection 10182..11555 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Kanamycin" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (Kanamycin)" /label="Kana" CDS 10467..11261 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="NptII" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; NptII" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; NptII (Kanamycin)" /label="NptII" primer_bind complement(10506..10525) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="59.5" /annotation_group="5983376779494000ae7c8f01-e258-4fab-b33e -4448bb492e7b" /created_by="primer3" /Sequence="AATAGCCTCTCCACCCAAGC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P25" primer_bind complement(11119..11138) /%GC="50.0" /Hairpin_Tm="46.1" /Self_Dimer_Tm="None" /Tm="59.2" /annotation_group="5983376722217300fbef089c-4f1d-46ca-8032 -c814476d7fbc" /created_by="primer3" /Sequence="AGCCAACGCTATGTCCTGAT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P24" terminator 11288..11555 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; NOST" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="tNOS" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /label="tNOS" T-DNA_border 11576..11908 /modified_by="wacae" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="LB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB" /label="LB" primer_bind complement(11820..11839) /%GC="50.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="59.9" /annotation_group="598337613680450047c58403-3fab-4b92-903e -e820c582942d" /created_by="primer3" /Sequence="TCAAACCCGGCAGCTTAGTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pK7WGC2" /label="P8" ORIGIN 1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag 61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca 121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca 181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat 241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc 301 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg 361 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt 421 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg 481 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta 541 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa 601 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg 661 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta 721 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta 781 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta 841 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat 901 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa 961 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg 1021 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta 1081 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg 1141 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt 1201 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct 1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 1381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 1441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 1621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 2041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg 2101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt 2161 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact 2221 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc 2281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga 2341 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg 2401 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc 2461 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc 2521 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat 2581 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa 2641 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc 2701 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac 2761 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct 2821 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag 2881 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg 2941 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg 3001 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg 3061 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga 3121 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc 3181 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc 3241 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc 3301 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt 3361 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg 3421 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc 3481 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc 3541 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc 3601 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag 3661 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc 3721 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac 3781 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc 3841 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg 3901 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga 3961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg 4021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc 4081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc 4141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc 4201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca 4261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt 4321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga 4381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag 4441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg 4501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa 4561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt 4621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg 4681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt 4741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg 4801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat 4861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg 4921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt 4981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt 5041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct 5101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac 5161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat 5221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc 5281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg 5341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg 5401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc 5461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca 5521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg 5581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg 5641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc 5701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc 5761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg 5821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt 5881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg 5941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga 6001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact 6061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact 6121 gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa 6181 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat 6241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg 6301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca 6361 atctgatcca agctcaagct aagcttgagc tctcccatat ggtcgactag agccaagctg 6421 atctcctttg ccccggagat caccatggac gactttctct atctctacga tctaggaaga 6481 aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa gattagcctc 6541 ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc ggcaggtctc 6601 atcaagacga tctacccgag taataatctc caggagatca aataccttcc caagaaggtt 6661 aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt 6721 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg 6781 caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa ggccatggag 6841 tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga acagttcata 6901 cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac 6961 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag 7021 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt 7081 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat 7141 aaaggaaagg ctatcgttca agatgcccct gccgacagtg gtcccaaaga tggaccccca 7201 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 7261 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac 7321 ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa 7381 ttaccacaac aaaacaaaca acaaacaaca ttacaattta ctattctagt cgacctgcag 7441 gcggccgcac tagtatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 7501 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 7561 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 7621 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc 7681 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 7741 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 7801 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 7861 acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg 7921 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca 7981 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 8041 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 8101 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 8161 agctgtacaa ggatatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata 8221 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa 8281 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc 8341 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa 8401 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca 8461 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt 8521 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc 8581 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat 8641 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga 8701 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct 8761 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg 8821 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga 8881 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta 8941 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga 9001 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg 9061 cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg 9121 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag 9181 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc 9241 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc 9301 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc 9361 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt 9421 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat 9481 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca 9541 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg 9601 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc 9661 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg 9721 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt 9781 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat 9841 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatatccc gcggccatgc 9901 tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca 9961 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat 10021 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat 10081 aaaatttcta attcctaaaa ccaaaatcca gtgacctgca ggcatgcgac gtcgggccct 10141 ctagaggatc cccgggtacc gcgaattatc gatcatgagc ggagaattaa gggagtcacg 10201 ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc 10261 aacgttgaag gagccactga gccgcgggtt tctggagttt aatgagctaa gcacatacgt 10321 cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt 10381 cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc 10441 atattcactc tcaactcgat cgaggcatga ttgaacaaga tggattgcac gcaggttctc 10501 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 10561 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 10621 acctgtccgg tgccctgaat gaactccaag acgaggcagc gcggctatcg tggctggcca 10681 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 10741 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 10801 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 10861 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 10921 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 10981 ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacccac ggcgatgcct 11041 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 11101 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 11161 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 11221 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgg 11281 actctagcta gagtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga 11341 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg 11401 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc 11461 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat 11521 tatcgcgcgc ggtgtcatct atgttactag atcgaccggc atgcaagctg ataattcaat 11581 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc 11641 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag 11701 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg 11761 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca 11821 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac 11881 gatgacagag cgttgctgcc tgtgatca //