LOCUS p2CGW7 6667 bp DNA circular 12-JUL-2022
DEFINITION .
ACCESSION urn.local...1g1-ds8rxib
KEYWORDS .
SOURCE
ORGANISM .
COMMENT http://www.informaxinc.com/
This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDATE|342187264|
VNTDBDATE|457854304|
LSOWNER|
VNTNAME|p2CGW7,0|
VNTAUTHORNAME|Demo User|
VNTAUTHOREML|manka@psb.ugent.be|
VNTOAUTHORNAME|Mansour Karimi|
VNTOAUTHORTEL|+3193313850|
VNTOAUTHOREML|mansour.karimi@psb.ugent.be|
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#297=(CGroupWidget
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#300=(CGroupWidget
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#303=(CGroupWidget
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#306=(CGroupWidget
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#309=(CGroupWidget
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#312=(CGroupWidget
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#317=(CGroupWidget
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(CObjectList)) #327=(CGroupWidget (CWidget
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(CObjectList) (CObjectList))) (CObjectList))) (CSeqView 10
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(CStringList) 57915532 268786344 (CObList))).
FEATURES Location/Qualifiers
promoter 1..1047
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCaMV 35S - Gateway"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Gateway)"
/label="p35S"
primer_bind complement(731..752)
/%GC="45.5"
/Hairpin_Tm="41.9"
/Self_Dimer_Tm="12.2"
/Tm="59.9"
/annotation_group="570592232089350088887fed-d264-4caa-9e6a
-2fce19a608d7"
/created_by="primer3"
/Sequence="CGCAATGATGGCATTTGTAGGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2CGW7"
/label="P18"
CDS 1069..1785
/label="ECFP"
primer_bind complement(1568..1587)
/#_Off-target_Sites=0
/Off-target_Template="p2CGW7"
/Sequence="CTCGATGTTGTGGCGGATCT"
/Tm="60.2"
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P33"
attR1 1792..1916
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR1"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1"
promoter 1941..1971
/note="Lac promoter of Escherichia coli containing 2 base
pair mutations"
/regulatory_class="promoter"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="lacUV5"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
(CmR)"
/label="lacUV5"
misc_feature 2025..2684
/Transferred_From="cmR"
/Transferred_Similarity="99.85%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; CmR"
/label="CmR"
/note="Geneious type: Bacterial Selection"
primer_bind complement(2255..2274)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="570592220226310003ad895b-66cf-4195-aece
-4ca36cf8034e"
/created_by="primer3"
/Sequence="TCACCAGCTCACCGTCTTTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2CGW7"
/label="P16"
UTR 2707..3025
/Mismatches=1
/%_Identity="99.68652037617555"
/Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
5'UTR (ccdB)"
/label="ccdB 5'UTR"
CDS 3026..3331
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="99.67%"
/Primary_Match="99.67%; Adjusted-Interval-0:0/0; ccdB"
/label="ccdB"
primer_bind complement(3051..3071)
/%GC="47.6"
/Hairpin_Tm="45.0"
/Self_Dimer_Tm="None"
/Tm="57.6"
/annotation_group="57059221426821009655c36a-29cc-49d0-8c94
-9ca00bdfb237"
/created_by="primer3"
/Sequence="GACGATAACGGCTCTCTCTTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2CGW7"
/label="P15"
attR2 complement(3372..3496)
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR2"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2"
terminator 3506..3731
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="t35S"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S"
/label="t35S"
primer_bind complement(3718..3737)
/%GC="55.0"
/Hairpin_Tm="44.4"
/Self_Dimer_Tm="32.8"
/Tm="60.6"
/annotation_group="57059222618393005a3fcc87-46c8-4f24-975b
-b5474cbf2af5"
/created_by="primer3"
/Sequence="GCCTGCAGGTCACTGGATTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2CGW7"
/label="P17"
rep_origin 3943..4398
/note="f1 replication origin derived from bacteriophage
f1"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="F1"
/Transferred_Similarity="99.34%"
/Primary_Match="99.34%; Adjusted-Interval-0:0/0; F1"
/label="F1"
promoter 4476..4580
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
primer_bind complement(4524..4545)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="18.4"
/Tm="60.2"
/annotation_group="570592249934230067c62a70-d152-4990-96cd
-e9d4baf7b372"
/created_by="primer3"
/Sequence="AGGGTTATTGTCTCATGAGCGG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2CGW7"
/label="P21"
misc_feature 4581..5441
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="99.77%"
/Primary_Match="99.77%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
primer_bind complement(5107..5126)
/%GC="55.0"
/Hairpin_Tm="40.4"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="5705922439129600dfdd6f9d-bb1e-4719-b77f
-6b44b3661ba1"
/created_by="primer3"
/Sequence="TACAGGCATCGTGGTGTCAC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2CGW7"
/label="P20"
rep_origin 5612..6200
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer_bind complement(5792..5811)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="57059218179956003c203475-2d7e-4339-898f
-1345680462c4"
/created_by="primer3"
/Sequence="GCGGTGCTACAGAGTTCTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2CGW7"
/label="P6"
primer_bind complement(6553..6572)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="57059223802493006c26428e-20a7-43a1-b8fe
-4b4456ce5531"
/created_by="primer3"
/Sequence="TCCGCTCACAATTCCACACA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="p2CGW7"
/label="P19"
ORIGIN
1 gagctctccc atatggtcga ctagagccaa gctgatctcc tttgccccgg agatcaccat
61 ggacgacttt ctctatctct acgatctagg aagaaagttc gacggagaag gtgacgatac
121 catgttcacc accgataatg agaagattag cctcttcaat ttcagaaaga atgctgaccc
181 acagatggtt agagaggcct acgcggcagg tctcatcaag acgatctacc cgagtaataa
241 tctccaggag atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac
301 taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt
361 atggacgatt caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa
421 gaaagtagtt cctactgaat caaaggccat ggagtcaaaa attcagatcg aggatctaac
481 agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa
541 gaagaaaatc ttcgtcaaca tggtggagca cgacactctc gtctactcca agaatatcaa
601 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg
661 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
721 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
781 ccctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
841 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
901 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
961 tcatttggag aggactccgg tatttttaca acaattacca caacaaaaca aacaacaaac
1021 aacattacaa tttactattc tagtcgacct gcaggcggcc gcactagtat ggtgagcaag
1081 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
1141 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
1201 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
1261 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
1321 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
1381 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
1441 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
1501 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
1561 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
1621 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
1681 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
1741 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggatat cacaagtttg
1801 tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
1861 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg
1921 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
1981 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
2041 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
2101 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
2161 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
2221 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
2281 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
2341 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
2401 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
2461 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
2521 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
2581 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
2641 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct
2701 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
2761 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac
2821 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
2881 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
2941 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
3001 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag
3061 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
3121 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
3181 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
3241 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
3301 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
3361 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
3421 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
3481 tcttgtacaa agtggtgata tcccgcggcc atgctagagt ccgcaaaaat caccagtctc
3541 tctctacaaa tctatctctc tctatttttc tccagaataa tgtgtgagta gttcccagat
3601 aagggaatta gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag
3661 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
3721 tccagtgacc tgcaggcatg cgacgtcggg cccaattcgc cctatagtga gtcgtattac
3781 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
3841 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
3901 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
3961 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
4021 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
4081 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
4141 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
4201 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
4261 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
4321 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
4381 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
4441 acaccgcatc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
4501 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
4561 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
4621 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
4681 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
4741 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
4801 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
4861 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
4921 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
4981 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
5041 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
5101 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
5161 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
5221 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
5281 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
5341 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
5401 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
5461 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
5521 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
5581 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
5641 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
5701 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
5761 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
5821 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
5881 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
5941 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
6001 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
6061 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
6121 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
6181 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
6241 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
6301 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
6361 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
6421 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
6481 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
6541 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
6601 accatgatta cgccaagcta tttaggtgac actatagaat actcaagcta tgcatccaac
6661 gcgttgg
//