LOCUS pH7WG2 11582 bp DNA circular 13-FEB-2023 DEFINITION . ACCESSION urn.local...18q-ds8iq9z KEYWORDS . SOURCE ORGANISM . COMMENT http://www.informaxinc.com/ This file is created by Vector NTI http://www.invitrogen.com/ ORIGDB|GenBank VNTDATE|342011011| VNTDBDATE|455677296| LSOWNER| VNTNAME|pH7WG2,0| VNTAUTHORNAME|Demo User| VNTAUTHOREML|manka@psb.ugent.be| VNTOAUTHORNAME|Mansour Karimi| VNTOAUTHORTEL|+3193313850| VNTOAUTHOREML|mansour.karimi@psb.ugent.be| Vector_NTI_Display_Data_(Do_Not_Edit!) 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Marker (2 total)") #67=(CFolderPar (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) (CObjectList #68=(CFolderPar (CGroupPar (CParagraph 1985 (3 #28# 0) 1 2 2 0 194) (CObjectList #69=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 1985 End: 3011 (Complementary)" 1) #70=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #71=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(1985..3011)" 1))) "p35S"))) "Promoter Eukaryotic (1 total)") #72=(CFolderPar (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) (CObjectList #73=(CFolderPar (CGroupPar (CParagraph 18 (3 #27# 0) 1 2 2 0 194) (CObjectList #74=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 18 End: 243 " 1) #75=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #76=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 18..243" 1))) "T35S"))) "Terminator (1 total)") #77=(CFolderPar (CGroupPar (CParagraph 86 (7 86 0) 1 1 1 0 178) (CObjectList #78=(CFolderPar (CGroupPar (CParagraph 253 (3 #25# 0) 1 2 2 0 194) (CObjectList #79=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 253 End: 377 (Complementary)" 1) #80=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #81=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(253..377)" 1))) "attR2") #82=(CFolderPar (CGroupPar (CParagraph 1833 (3 #24# 0) 1 2 2 0 194) (CObjectList #83=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 1833 End: 1957 (Complementary)" 1) #84=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #85=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(1833..1957)" 1))) "attR1"))) "Misc. Recombination (2 total)") #86=(CFolderPar (CGroupPar (CParagraph 87 (7 87 0) 1 1 1 0 178) (CObjectList #87=(CFolderPar (CGroupPar (CParagraph 3032 (3 #29# 0) 1 2 2 0 194) (CObjectList #88=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 3032 End: 3231" 1) #89=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #90=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 3032..3231" 1))) "RB") #91=(CFolderPar (CGroupPar (CParagraph 9390 (3 #31# 0) 1 2 2 0 194) (CObjectList #92=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 9390 End: 9722" 1) #93=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #94=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 9390..9722" 1))) "LB"))) "Misc. Signal (2 total)"))) "Feature Map") #95=(CFolderPar (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) (CObjectList #96=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50377904 (8 0) 1 1 1 0 178) (CObjectList #97=(CGroupPar (CParagraph 0 (10 #0# 0) 1 2 2 0 180) (CObjectList #98=(CLinePar (CParagraph 0 (1 #0# 1) 1 2 2 0 191) " N1: 7 " 1) #99=(CLinePar (CParagraph 0 (1 #0# 2) 1 2 2 0 191) " N2: 11194 " 1))))) "AatII: 2 sites") 5 1 "GACGTC" "CTGCAG") #100=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50377872 (8 0) 1 1 1 0 178) (CObjectList #101=(CGroupPar (CParagraph 0 (10 #1# 0) 1 2 2 0 180) (CObjectList #102=(CLinePar (CParagraph 0 (1 #1# 1) 1 2 2 0 191) " N1: 1 " 1))))) "ApaI: 1 site") 5 1 "GGGCCC" "CCCGGG") #103=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50377840 (8 0) 1 1 1 0 178) (CObjectList #104=(CGroupPar (CParagraph 0 (10 #2# 0) 1 2 2 0 180) (CObjectList #105=(CLinePar (CParagraph 0 (1 #2# 1) 1 2 2 0 191) " N1: 10016 " 1))))) "AseI: 1 site") 2 4 "ATTAAT" "TAATTA") #106=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50377808 (8 0) 1 1 1 0 178) (CObjectList)) "AvaI: 8 sites") 1 5 "CYCGRG" "GRGCYC") #107=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50377776 (8 0) 1 1 1 0 178) (CObjectList)) "BamHI: 3 sites") 1 5 "GGATCC" "CCTAGG") #108=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50391768 (8 0) 1 1 1 0 178) (CObjectList)) "BglII: 0 sites") 1 5 "AGATCT" "TCTAGA") #109=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50377744 (8 0) 1 1 1 0 178) (CObjectList #110=(CGroupPar (CParagraph 0 (10 #5# 0) 1 2 2 0 180) (CObjectList #111=(CLinePar (CParagraph 0 (1 #5# 1) 1 2 2 0 191) " N1: 6286 " 1))))) "ClaI: 1 site") 2 4 "ATCGAT" "TAGCTA") #112=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50377712 (8 0) 1 1 1 0 178) (CObjectList #113=(CGroupPar (CParagraph 0 (10 #6# 0) 1 2 2 0 180) (CObjectList #114=(CLinePar (CParagraph 0 (1 #6# 1) 1 2 2 0 191) " N1: 1507 " 1))))) "EcoRI: 1 site") 1 5 "GAATTC" "CTTAAG") #115=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50377680 (8 0) 1 1 1 0 178) (CObjectList)) "EcoRV: 3 sites") 3 3 "GATATC" "CTATAG") #116=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50377648 (8 0) 1 1 1 0 178) (CObjectList #117=(CGroupPar (CParagraph 0 (10 #8# 0) 1 2 2 0 180) (CObjectList #118=(CLinePar (CParagraph 0 (1 #8# 1) 1 2 2 0 191) " N1: 3032 " 1))))) "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") #119=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50377480 (8 0) 1 1 1 0 178) (CObjectList)) "NcoI: 3 sites") 1 5 "CCATGG" "GGTACC") #120=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50378336 (8 0) 1 1 1 0 178) (CObjectList)) "NdeI: 4 sites") 2 4 "CATATG" "GTATAC") #121=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50378304 (8 0) 1 1 1 0 178) (CObjectList)) "NotI: 5 sites") 2 6 "GCGGCCGC" "CGCCGGCG") #122=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50378136 (8 0) 1 1 1 0 178) (CObjectList)) "NruI: 0 sites") 3 3 "TCGCGA" "AGCGCT") #123=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50378104 (8 0) 1 1 1 0 178) (CObjectList)) "PstI: 3 sites") 5 1 "CTGCAG" "GACGTC") #124=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50377936 (8 0) 1 1 1 0 178) (CObjectList #125=(CGroupPar (CParagraph 0 (10 #14# 0) 1 2 2 0 180) (CObjectList #126=(CLinePar (CParagraph 0 (1 #14# 1) 1 2 2 0 191) " N1: 3030 " 1))))) "SacI: 1 site") 5 1 "GAGCTC" "CTCGAG") #127=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50391512 (8 0) 1 1 1 0 178) (CObjectList #128=(CGroupPar (CParagraph 0 (10 #15# 0) 1 2 2 0 180) (CObjectList #129=(CLinePar (CParagraph 0 (1 #15# 1) 1 2 2 0 191) " N1: 245 " 1) #130=(CLinePar (CParagraph 0 (1 #15# 2) 1 2 2 0 191) " N2: 11403 " 1))))) "SacII: 2 sites") 4 2 "CCGCGG" "GGCGCC") #131=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50391544 (8 0) 1 1 1 0 178) (CObjectList)) "SalI: 3 sites") 1 5 "GTCGAC" "CAGCTG") #132=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 50391576 (8 0) 1 1 1 0 178) (CObjectList #133=(CGroupPar (CParagraph 0 (10 #17# 0) 1 2 2 0 180) (CObjectList 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1.9 0.123113 1) #293=(CLabel (CWidget 0 (0 0) 1 2 0 0 #197# 40803852 100) (LOGPEN 0 0 8437824) 1 (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34 "Arial") 1.15807 0.611111 0 "{\\i Xma}I (11526)" "@N (@S)" 5 -2.86157 13.8968 0 -10 3.62185 0.694805 #292#)) (CObjectList))) (CObjectList)) #294=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1058 100) (CObjectList) (CObjectList)) #295=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -510 100) (CObjectList) (CObjectList))) (CObjectList))) (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList) 14353184 (CStringList) 84672717 14353144 (CObList))). FEATURES Location/Qualifiers Promoter 8..282 /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /label="P1" misc_feature 283..1074 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" rep_origin 1320..1908 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.83%" /Primary_Match="99.83%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" regulatory complement(1944..2204) /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /label="pBR322 bom" rep_origin 2578..2772 /note="origin of replication from Pseudomonas pVS1 plasmid" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 oriV" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV" /label="pVS1 oriV" regulatory complement(2838..3911) /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" regulatory complement(4340..4969) /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" T-DNA_border 6155..6385 /modified_by="wacae" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="RB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; RB" /label="RB" promoter 6387..7433 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCaMV 35S - Gateway" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Gateway)" /label="p35S" attR1 7461..7585 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR1" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1" promoter 7610..7640 /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="lacUV5" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (CmR)" /label="lacUV5" misc_feature 7694..8353 /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" UTR 8376..8694 /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.37%" /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" CDS 8695..9000 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.67%" /Primary_Match="99.67%; Adjusted-Interval-0:0/0; ccdB" /label="ccdB" attR2 complement(9041..9165) /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2" terminator 9175..9400 /created_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="t35S" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S" /label="t35S" Plant_Selection 9465..11229 /modified_by="mamee" /X-Transferred_From="Hygromycin" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro (Hygromycin)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Hygromycin" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro (Hygromycin)" /label="Hygro" promoter 9465..9737 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pNOS" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Basta" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Kanamycin)" /label="pNOS" misc_feature 9490 /Original_Bases /label /note="Geneious type: Editing History Insertion" CDS 9752..10777 /modified_by="wacae" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Hyg" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hyg" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Hyg (Hygromycin)" /label="Hyg" terminator 10962..11229 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; NOST" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="tNOS" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /label="tNOS" T-DNA_border 11250..11582 /modified_by="wacae" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="LB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB" /label="LB" ORIGIN 1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag 61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca 121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca 181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat 241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc 301 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg 361 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt 421 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg 481 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta 541 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa 601 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg 661 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta 721 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta 781 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta 841 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat 901 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa 961 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg 1021 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta 1081 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg 1141 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt 1201 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct 1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 1381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 1441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 1621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 2041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg 2101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt 2161 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact 2221 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc 2281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga 2341 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg 2401 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc 2461 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc 2521 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat 2581 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa 2641 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc 2701 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac 2761 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct 2821 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag 2881 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg 2941 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg 3001 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg 3061 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga 3121 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc 3181 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc 3241 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc 3301 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt 3361 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg 3421 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc 3481 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc 3541 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc 3601 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag 3661 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc 3721 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac 3781 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc 3841 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg 3901 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga 3961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg 4021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc 4081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc 4141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc 4201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca 4261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt 4321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga 4381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag 4441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg 4501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa 4561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt 4621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg 4681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt 4741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg 4801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat 4861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg 4921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt 4981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt 5041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct 5101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac 5161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat 5221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc 5281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg 5341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg 5401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc 5461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca 5521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg 5581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg 5641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc 5701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc 5761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg 5821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt 5881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg 5941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga 6001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact 6061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact 6121 gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa 6181 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat 6241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg 6301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca 6361 atctgatcca agctcaagct aagcttgagc tctcccatat ggtcgactag agccaagctg 6421 atctcctttg ccccggagat caccatggac gactttctct atctctacga tctaggaaga 6481 aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa gattagcctc 6541 ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc ggcaggtctc 6601 atcaagacga tctacccgag taataatctc caggagatca aataccttcc caagaaggtt 6661 aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt 6721 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg 6781 caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa ggccatggag 6841 tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga acagttcata 6901 cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac 6961 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag 7021 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt 7081 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat 7141 aaaggaaagg ctatcgttca agatgcccct gccgacagtg gtcccaaaga tggaccccca 7201 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 7261 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac 7321 ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa 7381 ttaccacaac aaaacaaaca acaaacaaca ttacaattta ctattctagt cgacctgcag 7441 gcggccgcac tagtgatatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg 7501 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta 7561 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta 7621 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc 7681 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg 7741 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac 7801 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta 7861 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc 7921 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca 7981 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt 8041 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa 8101 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt 8161 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata 8221 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg 8281 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca 8341 gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt 8401 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa 8461 aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg 8521 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag 8581 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg 8641 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag 8701 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt 8761 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg 8821 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc 8881 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat 8941 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa 9001 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg 9061 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt 9121 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgatat cccgcggcca 9181 tgctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct 9241 ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct 9301 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc 9361 aataaaattt ctaattccta aaaccaaaat ccagtgacct gcaggcatgc gacgtcgggc 9421 cctctagagg atccccgggt accgcgaatt atcgatcatg agcggagaat taagggagtc 9481 acgttatgac ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac 9541 cgcaacgttg aaggagccac tgagccgcgg gtttctggag tttaatgagc taagcacata 9601 cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata 9661 tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt 9721 ctcatattca ctctcaactc gaatcccccc tatgaaaaag cctgaactca ccgcgacgtc 9781 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga 9841 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt 9901 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc 9961 cgcgctcccg attccggaag tgcttgacat tggggagttc agcgagagcc tgacctattg 10021 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc 10081 tgttcttcag ccggtcgcgg aggctatgga tgctatcgct gcggccgatc ttagccagac 10141 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt 10201 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt 10261 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga 10321 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg 10381 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc 10441 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga 10501 gcggaggcat ccggagcttg caggatcgcc aaggctccgg gcgtatatgc tccgcattgg 10561 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca 10621 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc 10681 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa 10741 ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt agatgccgac cgaacaagag 10801 ctgatttcga gaacgcctca gccagcaact cgcgcgagcc tagcaaggca aatgcgagag 10861 aacggcctta cgcttggtgg cacagttctc gtccacagtt cgctaagctc gctcggctgg 10921 gtcgcgggag ggccggtcgc agtgattcag gaattaattc cctagagtca agcagatcgt 10981 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt 11041 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg 11101 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata 11161 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta 11221 ctagatcgac cggcatgcaa gctgataatt caattcggcg ttaattcagt acattaaaaa 11281 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct 11341 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac 11401 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt 11461 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg 11521 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat 11581 ca //