LOCUS       pGGIB-U7-pOsU3-BsaI-ccdB/GmR-BsaI-U8        4690 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...b7-bfyraa3
VERSION     urn.local...b7-bfyraa3
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_difference 1..18
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     misc_feature    19..58
                     /created_by="lapau"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:6f-cctbnui"">U7</a>"
                     /label="U7"
                     /note="Geneious type: Golden Gibson Cloning site"
     Linker          71..119
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker III"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1i-c4nnd54"">Linker III</a>"
                     /label="Linker III"
     promoter        120..561
                     /modified_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="OsU3"
                     /Transferred_Similarity="97.79%"
                     /Primary_Match="97.79%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1u-c4nnd54"">OsU3</a>"
                     /label="OsU3"
     CDS             complement(575..880)
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.35%"
                     /Primary_Match="99.35%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:t-cj5879l"">ccdB</a>"
                     /label="ccdB"
     misc_feature    579
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    662
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    665
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     UTR             complement(881..1199)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:thjac:t-cj5879l"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    889
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1107
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1208..1233
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1238
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1239^1240
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    1462..1995
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2q-c4nnd56"">GmR</a>"
                     /label="GmR"
                     /note="Geneious type: Bacterial Selection"
     misc_feature    1754
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2124^2125
                     /Original_Bases="CGATC"
                     /label="CGATC"
                     /note="Geneious type: Editing History Deletion"
     promoter        complement(2151..2181)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     Scaffold        2205..2287
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpCas9 Scaffold"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Scaffold
                     (<a href=""urn:local:thjac:2f-c4nnd55"">SpCas9
                     Scaffold</a>)"
                     /label="Scaffold"
     misc_feature    2298..2337
                     /created_by="lapau"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:6h-cctbnuj"">U8</a>"
                     /label="U8"
                     /note="Geneious type: Golden Gibson Cloning site"
     misc_difference complement(2338..2355)
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     primer          complement(2383..2403)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2464..2514
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2515..3324
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2o-c4nnd56"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3471..4059
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4659..4679
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 attaccctgt tatccctaca agacgctggc tctgacattt ccgctactga actactcgct
       61 cgagatacct taagctaggt agtaactagt gcgactgaag cttatcgcgt atgcgagtga
      121 ctaagtaatt catccaggtc accaagttct aggattttca gaactgcaac ttattttatc
      181 aaggaatctt taaacatacg aacagatcac ttaaagttct tctgaagcaa cttaaagtta
      241 tcaggcatgc atggatcttg gaggaatcag atgtgcagtc agggaccata gcacaagaca
      301 ggcgtctact actggtgcta ccagcaaatg ctggaagccg ggaacactgg gtacgttgga
      361 aaccacgtga tgtgaagaag taagataaac tgtaggagaa aagcatttcg tagtgggcca
      421 tgaagccttt caggacatgt attgcagtat gggccggccc attacgcaat tggacgacaa
      481 caaagactag tattagtacc acctcggcta tccacataga tcaaagctga tttaaaagag
      541 ttgtgcagat gatccgtggc aggagaccgt cgacttatct tccccagaac atcaggttaa
      601 tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg
      661 aaaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca
      721 ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga
      781 tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat
      841 aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc cctgttctcg
      901 tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt
      961 tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc
     1021 agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg
     1081 tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg tatacatatc
     1141 agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt atctggcttt
     1201 tagtaagcct cgacgacgtc ggatccccat ggcccgggtc tagattgaca taagcctgtt
     1261 cggttcgtaa actgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     1321 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     1381 ttgtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     1441 tgatgttatg gagcagcaac gatgttacgc agcagcaacg atgttacgca gcagggcagt
     1501 cgccctaaaa caaagttagg tggctcaagt atgggcatca ttcgcacatg taggctcggc
     1561 cctgaccaag tcaaatccat gcgggctgct cttgatcttt tcggtcgtga gttcggagac
     1621 gtagccacct actcccaaca tcagccggac tccgattacc tcgggaactt gctccgtagt
     1681 aagacattca tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc tctcgcggct
     1741 tacgttctgc ccaagtttga gcagccgcgt agtgagatct atatctatga tctcgcagtc
     1801 tccggcgagc accggaggca gggcattgcc accgcgctca tcaatctcct caagcatgag
     1861 gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag attacggtga cgatcccgca
     1921 gtggctctct atacaaagtt gggcatacgg gaagaagtga tgcactttga tatcgaccca
     1981 agtaccgcca cctaacaatt cgttcaagcc gagatcggct tcccggccta atttcccctc
     2041 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     2101 tggcaaaagt ttatgcattt cttgcggctg atcctaactc aaaatccaca cattatacga
     2161 gccggaagca taaagtgtaa agcctggggt gcctaatggt ctcagtttta gagctagaaa
     2221 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     2281 tttttttgta ttctagacct cgtctcaacc aaagcaatca acccatcaac cacctggtag
     2341 ggataacagg gtaatgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     2401 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2461 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     2521 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     2581 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     2641 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2701 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     2761 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     2821 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     2881 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     2941 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     3001 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     3061 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     3121 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     3181 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     3241 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     3301 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     3361 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     3421 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3481 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3541 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3601 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3661 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3721 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3781 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3841 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3901 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3961 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4021 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4081 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4141 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4201 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4261 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4321 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     4381 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     4441 atggcgggcg tcctgcccgc caccccttcg ggccgttgct tcacaacgtt caaatccgct
     4501 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
     4561 gcccagtcat ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc
     4621 gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa
     4681 gctcgggccc
//