LOCUS pGGIB-U4-pZmPLTP-ZmBBM-tnos-U5 4941 bp DNA circular UNA 23-FEB-2023 DEFINITION . ACCESSION urn.local...8e-aruiqnr VERSION urn.local...8e-aruiqnr KEYWORDS . SOURCE . ORGANISM . FEATURES Location/Qualifiers misc_difference 1..18 /created_by="laurenspauwels" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="I-SceI site" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; I-SceI site" /label="I-SceI site" misc_feature 19..58 /created_by="lapau" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="U3" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; U3" /label="U3" /note="Geneious type: Golden Gibson Cloning site" promoter 71..1381 /created_by="lapau" /modified_by="lapau" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pZmAXIG" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pZmAXIG" /label="pZmAXIG" CDS 1386..2294 /created_by="lapau" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ZmWUS2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmWUS2" /label="ZmWUS2" terminator 2299..2538 /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="35ST" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 35ST" /label="35ST" misc_feature 2549..2588 /created_by="lapau" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="U4" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; U4" /label="U4" /note="Geneious type: Golden Gibson Cloning site" misc_difference complement(2589..2606) /created_by="laurenspauwels" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="I-SceI site" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; I-SceI site" /label="I-SceI site" primer complement(2634..2654) /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 2715..2765 /created_by="chgai" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 2766..3575 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 3722..4310 /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 4910..4930 /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 attaccctgt tatccctagc actgaaggtc ctcaatcgca ctggaaacat caaggtcgct 61 cgagatacct aggcgaccca tcgctgcttt gtctacatca tgttcttcat catcctcccc 121 aggcgacgcg tgctgctgtt cttattcaga ctaccgttcg agtgactgca tggcgtacat 181 ctttctgcat cgactttgta cggctacatc gaacatatac acgagatgtc tcgtgtgaat 241 agagtcacta atgccttaag catcggttac tccgtagggt acattctgtt cttcttattt 301 gtgcatattt ttattgttgt ttactgatta tacgagtagt tatacataca tgcacataca 361 tatcatcaca tatatcacaa tatttttcta aattaaatta aaactaaaaa tgactaaatt 421 tctaacacca acgacattgt aatgttttct ccaacaactt tacctattct acattgttct 481 atttcgaatt tcactctata aacaacatag tctacaatgg aaaacagtgc tttgtacgac 541 tatatacgcg atgtgtggct acaacataag acaatatagt cgtttgaaga ttgaacctat 601 atatcggtac ggttaatccg tctatgtacg tgggcatgac gaacacccgt gataacgaag 661 gattaacgtg cacaatcata aatccaaagt aggagcggtg catgatgaga atcgctctca 721 gtactcgaca taatgaacct tacgaggtac aacaggcagg caggcaggga ccaggggccg 781 cctttatttc aggctcgctg gccccacggg cgtgctgcgt gcacgaaggg cactacccca 841 acctctcacc gaaaaccgcg ctggatcggc aaatcaaacg aggtggtgcc ccgtgcccac 901 tctccacgtc cacggcacca tccctctgca gccgctcacc agccatgccg tgtcgcggaa 961 cggcacaacc acccccaacc cactcacgaa accccgtccc ggccgtgccc gtgtcggtcc 1021 gcgctcggca acgaggcggc ccgcgctgct gagtcccctg gacacccgac accctgtcgg 1081 ccctttgttt attcatcccg aaatctcatc tgcccccacg gccgactgcg ctgcgccgcc 1141 cggatatata tacccatcgt tatcgatcga tcgatcgcgt cactcacggg tagctcatgg 1201 tcgagcgtag catgcaggaa cttatttgcc gtgcgctccc agctctccgc tcgcgtgcct 1261 tccagtctgt ctcacactag ctgctgtggg acgatcgaag tgggtgtgtc agctagctag 1321 ctgcgccgtg accacgcaca tgaccgcagt gcgcgcgggg ctgatcaagg gaaagtgatc 1381 cggctatggc ggccaatgcg ggcggcggtg gagcgggagg aggcagcggc agcggcagcg 1441 tggctgcgcc ggcggtgtgc cgccccagcg gctcgcggtg gacgccgacg ccggagcaga 1501 tcaggatgct gaaggagctc tactacggct gcggcatccg gtcgcccagc tcggagcaga 1561 tccagcgcat caccgccatg ctgcggcagc acggcaagat cgagggcaag aacgtcttct 1621 actggttcca gaaccacaag gcccgcgagc gccagaagcg ccgcctcacc agcctcgacg 1681 tcaacgtgcc cgccgccggc gcggccgacg ccaccaccag ccaactcggc gtcctctcgc 1741 tgtcgtcgcc gccgccttca ggcgcggcgc ctccctcgcc caccctcggc ttctacgccg 1801 ccggcaatgg cggcggatcg gctgtgctgc tggacacgag ttccgactgg ggcagcagcg 1861 gcgctgccat ggccaccgag acatgcttcc tgcaggacta catgggcgtg acggacacgg 1921 gcagctcgtc gcagtggcca cgcttctcgt cgtcggacac gataatggcg gcggccgcgg 1981 cgcgggcggc gacgacgcgg gcgcccgaga cgctccctct cttcccgacc tgcggcgacg 2041 acggcggcag cggtagcagc agctacttgc cgttctgggg tgccgcgtcc acaactgccg 2101 gcgccacttc ttccgttgcg atccagcagc aacaccagct gcaggagcag tacagctttt 2161 acagcaacag caacagcacc cagctggccg gcaccggcaa ccaagacgta tcggcaacag 2221 cagcagcagc cgccgccctg gagctgagcc tcagctcatg gtgctcccct taccctgctg 2281 cagggagtat gtgatcagga taactagctg agcggccatg ctagagtccg caaaaatcac 2341 cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt 2401 cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa 2461 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa 2521 accaaaatcc agtgacctgt attctagact gacctcctgc cagcaatagt aagacaacac 2581 gcaaagtcta gggataacag ggtaatgata tcccctatag tgagtcgtat tacatggtca 2641 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag 2701 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg 2761 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg 2821 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct 2881 atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg 2941 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc 3001 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga 3061 tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg 3121 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt 3181 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg 3241 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag 3301 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac 3361 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg 3421 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc 3481 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat 3541 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac 3601 actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta 3661 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 3721 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 3781 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 3841 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 3901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 3961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 4021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 4081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 4141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 4201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 4261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 4321 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 4381 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 4441 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 4501 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 4561 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg 4621 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg 4681 cctggcagtt tatggcgggc gtcctgcccg ccaccccttc gggccgttgc ttcacaacgt 4741 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga 4801 taaaacgaaa ggcccagtca tccgactgag cctttcgttt tatttgatgc ctggcagttc 4861 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg 4921 gccagtctta agctcgggcc c //