LOCUS pGG-C-LoxP-ZmNLS-mScarlet-D 3206 bp DNA circular UNA 21-APR-2023 DEFINITION . ACCESSION VERSION . KEYWORDS . SOURCE . ORGANISM . FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2022.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="C" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; C" /label="C" Start 14..16 /created_by="lapau" /modified_by="mamee" /label="START" motif 17..50 /created_by="lapau" /note="Derived using Geneious Prime 2022.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="loxP" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; loxP" /label="LoxP" NLS 53..142 /created_by="lapau" /modified_by="mamee" /label="O2 NLS" CDS 143..841 /vntifkey=4 /note="Derived using Geneious Prime 2022.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="mScarlet" /Transferred_Similarity="99.86%" /Adjusted_Transferred_Similarity="99.43%" /Primary_Match="99.86%; Adjusted-Interval-0:0/-3 (adjustedto 99.43%; Adjusted-Interval-0:0/0); mScarlet-L (mScarlet)" /Alternative_Match="89.97%; Adjusted-Interval-0:0/-3 (adjusted to 89.59%; Adjusted-Interval-0:0/0); mCherry" /Alternative_Match="67.24%; Adjusted-Interval-0:3/-3 (adjusted to 66.67%; Adjusted-Interval-0:0/0); TagRFP" /Alternative_Match="63.43%; Adjusted-Interval-0:0/-3 (adjusted to 63.17%; Adjusted-Interval-0:0/0); mRuby3" /modified_by="mamee" /label="mScarlet-L*" GG complement(842..852) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2022.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="D" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; D" /Alternative_Match="90.91%; Adjusted-Interval-0:0/0; E" /label="D" rep_origin complement(1083..1671) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2022.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" misc_feature complement(1842..2702) /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="99.42%" /Primary_Match="99.42%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" promoter complement(2703..2807) /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" ORIGIN 1 ggtctcaggc tccatgataa cttcgtatag catacattat acgaagttat cccctaccga 61 ggaaagagtg aggaaaagaa aggaatccaa tagagaatca gccagacgct cgagatacag 121 gaaagccgct cacctgaaag aactggtgag caagggcgag gcagtgatca aggagttcat 181 gcggttcaag gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga 241 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg 301 ccccctgccc ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt 361 caccaagcac cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa 421 gtgggagcgc gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc 481 cctggaggac ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga 541 cggccccgta atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc 601 cgaggacggc gtgctgaagg gcgacattaa gatggccctg cgcctgaagg acggcggccg 661 ctacctggcg gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc 721 ctacaacgtc gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga 781 acagtacgaa cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagtg 841 atcagtgaga ccgatatcct gggcctcatg ggccttccgc tcactgcccg ctttccagtc 901 gggaaacctg tcgtgccagc tgcattaaca tggtcatagc tgtttccttg cgtattgggc 961 gctctccgct tcctcgctca ctgactcgct gcgctcggtc gttcgggtaa agcctggggt 1021 gcctaatgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 1081 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 1141 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 1201 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 1261 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 1321 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 1381 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 1441 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 1501 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 1561 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 1621 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 1681 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 1741 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 1801 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 1861 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 1921 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 1981 cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 2041 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 2101 gaagctagag taagtagttc gccagttaat agtttgcgca atgttgttgc cattgctacg 2161 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 2221 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 2281 ccgatcgttg tcagaagtaa gttggccgca gtgttatcgc tcatggttat ggcagcactg 2341 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 2401 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 2461 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 2521 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 2581 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 2641 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 2701 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 2761 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 2821 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt 2881 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaaa 2941 gaatagaccg agatagggtt gagtggccgc tacagggcgc tcccattcgc cattcaggct 3001 gcgcaactgt tgggaagggc gtttcggtgc gggcctcttc gctattacgc cagctggcga 3061 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 3121 gttgtaaaac gacggccagt gagcgcgacg taatacgact cactataggg cgaattggcg 3181 gaaggccgtc aaggccgcat gatatc //