LOCUS pGG-C-ZmBBM-D 4821 bp DNA circular UNA 21-APR-2023 DEFINITION . ACCESSION urn.local...4p2-95xpshg VERSION urn.local...4p2-95xpshg KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="C" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; C" /label="C" CDS 14..2146 /created_by="lapau" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ZmODP2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmODP2" /modified_by="mamee" /label="ZmODP2*" GG complement(2147..2157) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="D" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; D" /Alternative_Match="90.91%; Adjusted-Interval-0:0/0; E" /label="D" misc_feature 2150^2151 /Original_Bases="TCAG" /label="TCAG" /note="Geneious type: Editing History Deletion" primer complement(2177..2197) /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 2677..2781 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 2782..3642 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 3813..4401 /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 4765..4786 /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcaggc tccatggcca ctgtgaacaa ctggctcgct ttctccctct ccccgcagga 61 gctgccgccc tcccagacga cggactccac actcatctcg gccgccaccg ccgaccatgt 121 ctccggcgat gtctgcttca acatccccca agattggagc atgaggggat cagagctttc 181 ggcgctcgtc gcggagccga agctggagga cttcctcggc ggcatctcct tctccgagca 241 gcatcacaag gccaactgca acatgatacc cagcactagc agcacagttt gctacgcgag 301 ctcaggtgct agcaccggct accatcacca gctgtaccac cagcccacca gctcagcgct 361 ccacttcgcg gactccgtaa tggtggcctc ctcggccggt gtccacgacg gcggtgccat 421 gctcagcgcg gccgccgcta acggtgtcgc tggcgctgcc agtgccaacg gcggcggcat 481 cgggctgtcc atgattaaga actggctgcg gagccaaccg gcgcccatgc agccgagggt 541 ggcggcggct gagggcgcgc aggggctctc tttgtccatg aacatggcgg ggacgaccca 601 aggcgctgct ggcatgccac ttctcgctgg agagcgcgca cgggcgcccg agagtgtatc 661 gacgtcagca cagggtggag ccgtcgtcgt cacggcgccg aaggaggata gcggtggcag 721 cggtgttgcc ggcgctctag tagccgtgag cacggacacg ggtggcagcg gcggcgcgtc 781 ggctgacaac acggcaagga agacggtgga cacgttcggg cagcgcacgt cgatttaccg 841 tggcgtgaca aggcatagat ggactgggag atatgaggca catctttggg ataacagttg 901 cagaagggaa gggcaaactc gtaagggtcg tcaagtctat ttaggtggct atgataaaga 961 ggagaaagct gctagggctt atgatcttgc tgctctgaag tactggggtg ccacaacaac 1021 aacaaatttt ccagtgagta actacgaaaa ggagctcgag gacatgaagc acatgacaag 1081 gcaggagttt gtagcgtctc tgagaaggaa gagcagtggt ttctccagag gtgcatccat 1141 ttacagggga gtgactaggc atcaccaaca tggaagatgg caagcacgga ttggacgagt 1201 tgcagggaac aaggatcttt acttgggcac cttcagcacc caggaggagg cagcggaggc 1261 gtacgacatc gcggcgatca agttccgcgg cctcaacgcc gtcaccaact tcgacatgag 1321 ccgctacgac gtgaagagca tcctggacag cagcgccctc cccatcggca gcgccgccaa 1381 gcgcctcaag gaggccgagg ccgcagcgtc cgcgcagcac caccacgccg gcgtggtgag 1441 ctacgacgtc ggccgcatcg cctcgcagct cggcgacggc ggagccctgg cggcggcgta 1501 cggcgcgcac taccacggcg ccgcctggcc gaccatcgcg ttccagccgg gcgccgccag 1561 cacaggcctg taccacccgt acgcgcagca gccaatgcgc ggcggcgggt ggtgcaagca 1621 ggagcaggac cacgcggtga tcgcggccgc gcacagcctg caggacctcc accacctgaa 1681 cctgggcgcg gccggcgcgc acgacttttt ctcggcaggg cagcaggccg ccgccgctgc 1741 gatgcacggc ctgggtagca tcgacagtgc gtcgctcgag cacagcaccg gctccaactc 1801 cgtcgtctac aacggcgggg tcggcgacag caacggcgcc agcgccgtcg gcggcagtgg 1861 cggtggctac atgatgccga tgagcgctgc cggagcaacc actacatcgg caatggtgag 1921 ccacgagcag gtgcatgcac gggcctacga cgaagccaag caggctgctc agatggggta 1981 cgagagctac ctggtgaacg cggagaacaa tggtggcgga aggatgtctg catgggggac 2041 tgtcgtgtct gcagccgcgg cggcagcagc aagcagcaac gacaacatgg ccgccgacgt 2101 cggccatggc ggcgcgcagc tcttcagtgt ctggaacgac acttaatcag tgagaccgaa 2161 ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa 2221 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 2281 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 2341 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg 2401 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca 2461 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 2521 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 2581 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt 2641 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 2701 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 2761 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 2821 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 2881 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 2941 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 3001 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 3061 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 3121 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 3181 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 3241 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 3301 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 3361 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 3421 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 3481 ggagccggtg agcgtggctc tcgaggtatc attgcagcac tggggccaga tggtaagccc 3541 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 3601 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 3661 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 3721 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 3781 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 3841 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 3901 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 3961 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 4021 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 4081 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 4141 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 4201 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 4261 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 4321 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 4381 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 4441 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 4501 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 4561 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 4621 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 4681 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 4741 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacgag ctatttaggt 4801 gacactatag aagtgaagct t //