LOCUS pEN-R2-pBdEF1a-GUS-35ST-L3 6745 bp DNA circular UNA 23-FEB-2023 DEFINITION . ACCESSION urn.local...3l8-95xprmj VERSION urn.local...3l8-95xprmj KEYWORDS . SOURCE . ORGANISM . FEATURES Location/Qualifiers attR2 complement(1..124) /modified_by="mamee" /label="attR2" promoter 131..2130 /modified_by="lapau" /label="pBdEF1a.1" regulatory 2131..2133 /created_by="lapau" /label="ATG" CDS 2173..3982 /vntifkey=4 /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Gus-intron" /Transferred_Similarity="99.78%" /Primary_Match="99.78%; Adjusted-Interval-0:0/0, Adjusted-Interval-1:0/0; Gus-intron" /modified_by="lapau" /label="GUS" terminator 3986..4225 /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="35ST" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 35ST" /label="35ST" attL3 complement(4231..4327) /modified_by="mamee" /label="attL3" primer complement(4384..4404) /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 4465..4515 /created_by="chgai" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 4516..5325 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 5472..6060 /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.49%" /Primary_Match="99.49%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 6659..6679 /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc 61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa 121 gtggctacct agatttgtga ttgttcagct acatgctcct tgaagcacct ttattctact 181 ataaattagt tcgttggacg agactattag accacatgct agccatgctt gttcgtagtt 241 gtcttcaccg ccattgcaaa aattgtcaat aaatatttag agtgggtggc atcagaaaaa 301 catctctagt ggactctctt cctatcatag ctactcgggc tgtagataga acgagggcac 361 aagagttggg tggcgtaggt ttactcgtga cctcaactct tttggctgtg tcttacgtct 421 aagatgggtt tggcatgtga gaaacatagg tctaagcaat tcatgttagg gctgttgcat 481 tgttgttgca tcaaccaaat gtccagatag cagttcatgc tacatctagt tgaaaaccct 541 catcattagg cggaacatgt gttctttttt agcatagtca aagtcagatt gcggcactcg 601 ctcatccacg gaaagaattt tccctgtgca ggcatctcga tcaaaagacg caaattaatt 661 tttgaatagc gatataacaa tatctaatta acgtttcttg ttttctgcga aatgtctttc 721 atcataaaat gagtcatctc gatgagccca agtgacatag cccaacaccc caccccacca 781 ataaaagtga agaaaacatg ttgggaaaac tataccaagt aaaatacgag ttgttctaaa 841 gaaaaagtaa agtacgagtt agatcgcacc ctgtcctgga gtgtggcttg atgatccaac 901 tcctagcatt gtatccctgt ttttggatga tgtaactatt atttacaatg aataaagagg 961 tgttttacta gtaaaaaaat cttgagggga ggagaaaata atggaggtct tttttcaaac 1021 cgatggacta ttatttttag tgaaagagaa taatattatt ggaaaaatta ttctatccac 1081 ttattttata ttggcagaat acaaagaatg gtggggtcca cgcggaactt gcggcccccg 1141 aaacctatcg agggcgcggt acccaagcaa ggaacggagg aaacttgcgg ggcccgaaac 1201 ctagtgataa aaggcatatc atccacacga tgaagatctg acggaccata tctcccacca 1261 cggaaagcca tcagacgagg atcagacggc caggaaggaa ccctagcgcc cgccggtgcc 1321 aatataaagc gccactctct ctcgtcttaa gccccagcct ctccattccc ctctccctct 1381 cgccgccgcc gtctccttct cctactccct tcgaggtgtg ttgttcatcc gtcccgaatc 1441 catccatccc ctcttcagat gtgttgttca tggctctaat agctctagat ctgcttgttt 1501 gtgttgttta gctctagatc tactcgcgcg cgcttctctc tcgatctcct gtagaacaat 1561 tttggttggt tttttgtgca tatccatggt aattttgtct gcaatatgga ggaggctttc 1621 taagctccta cgtagcatcg atctttagaa ttccctcggt ttctgtttat ttcttcgcga 1681 gggctctctg ttatctgtag gagtagctgt aagcgcggtt cgttacggat taatcgtcat 1741 gcttagttga acctatcggt cgaaggattt gtgtgggttg tcgtgtagaa ttgacaccat 1801 ctacttactg tactgatatg ccgatctgta ggatactctt cattactttt gtttactgct 1861 agttgtggtg tagatttagc attctcaaac ccatgctgta gcgtttctaa tattgttaca 1921 tagatctacc ggtgcctgtt aattgtattc gatcgggcgt ttctacatct gtccgcccac 1981 ctagttttat atgtggtaat caaaattgcg ttgacttcgt gatgctgtct gtgtactgtt 2041 tttaatcgct cttacttaga tgatcaacat ggtgatggtt acgatttact gttttctaat 2101 ccctgttact tcgatgctgc agtttcagcc atgacaacag tattcagtcg actggtacca 2161 acaggctcaa caatggtccg tcctgtagaa accccaaccc gtgaaatcaa aaaactcgac 2221 ggcctgtggg cattcagtct ggatcgcgaa aactgtggaa ttgatcagcg ttggtgggaa 2281 agcgcgttac aagaaagccg ggcaattgct gtgccaggca gttttaacga tcagttcgcc 2341 gatgcagata ttcgtaatta tgcgggcaac gtctggtatc agcgcgaagt ctttataccg 2401 aaaggttggg caggccagcg tatcgtgctg cgtttcgatg cggtcactca ttacggcaaa 2461 gtgtgggtca ataatcagga agtgatggag catcagggcg gctatacgcc atttgaagcc 2521 gatgtcacgc cgtatgttat tgccgggaaa agtgtacgta tcaccgtttg tgtgaacaac 2581 gaactgaact ggcagactat cccgccggga atggtgatta ccgacgaaaa cggcaagaaa 2641 aagcagtctt acttccatga tttctttaac tatgccggaa tccatcgcag cgtaatgctc 2701 tacaccacgc cgaacacctg ggtggacgat atcaccgtgg tgacgcatgt cgcgcaagac 2761 tgtaaccacg cgtctgttga ctggcaggtg gtggccaatg gtgatgtcag cgttgaactg 2821 cgtgatgcgg atcaacaggt ggttgcaact ggacaaggca ctagcgggac tttgcaagtg 2881 gtgaatccgc acctctggca accgggtgaa ggttatctct atgaactgtg cgtcacagcc 2941 aaaagccaga cagagtgtga tatctacccg cttcgcgtcg gcatccggtc agtggcagtg 3001 aagggcgaac agttcctgat taaccacaaa ccgttctact ttactggctt tggtcgtcat 3061 gaagatgcgg acttgcgtgg caaaggattc gataacgtgc tgatggtgca cgaccacgca 3121 ttaatggact ggattggggc caactcctac cgtacctcgc attaccctta cgctgaagag 3181 atgctcgact gggcagatga acatggcatc gtggtgattg atgaaactgc tgctgtcggc 3241 tttaacctct ctttaggcat tggtttcgaa gcgggcaaca agccgaaaga actgtacagc 3301 gaagaggcag tcaacgggga aactcagcaa gcgcacttac aggcgattaa agagctgata 3361 gcgcgtgaca aaaaccaccc aagcgtggtg atgtggagta ttgccaacga accggatacc 3421 cgtccgcaag gtgcacggga atatttcgcg ccactggcgg aagcaacgcg taaactcgac 3481 ccgacgcgtc cgatcacctg cgtcaatgta atgttctgcg acgctcacac cgataccatc 3541 agcgatctct ttgatgtgct gtgcctgaac cgttattacg gatggtatgt ccaaagcggc 3601 gatttggaaa cggcagagaa ggtactggaa aaagaacttc tggcctggca ggagaaactg 3661 catcagccga ttatcatcac cgaatacggc gtggatacgt tagccgggct gcactcaatg 3721 tacaccgaca tgtggagtga agagtatcag tgtgcatggc tggatatgta tcaccgcgtc 3781 tttgatcgcg tcagcgccgt cgtcggtgaa caggtatgga atttcgccga ttttgcgacc 3841 tcgcaaggca tattgcgcgt tggcggtaac aagaaaggga tcttcactcg cgaccgcaaa 3901 ccgaagtcgg cggcttttct gctgcaaaaa cgctggactg gcatgaactt cggtgaaaaa 3961 ccgcagcagg gaggcaaaca atcaggataa ctagctgagc ggccatgcta gagtccgcaa 4021 aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg 4081 agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata 4141 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat 4201 tcctaaaacc aaaatccagt gacctgtatg caactttatt atacaaagtt ggcattataa 4261 aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca 4321 ttatttggag ctccatggta gcgttaacgc ggccgcgata tcccctatag tgagtcgtat 4381 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac 4441 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt 4501 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc 4561 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt 4621 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc 4681 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa 4741 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc 4801 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt 4861 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt 4921 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat 4981 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa 5041 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt 5101 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt 5161 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt 5221 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat 5281 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat 5341 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca 5401 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc 5461 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 5521 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 5581 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 5641 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 5701 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 5761 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 5821 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg 5881 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 5941 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 6001 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 6061 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 6121 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 6181 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 6241 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 6301 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc 6361 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct 6421 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct 6481 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca 6541 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc 6601 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt 6661 aaaacgacgg ccagtcttaa gctcgggccc tgcagctcta gagctcgaat tctacaggtc 6721 actaatacca tctaagtagt tgatt //