LOCUS pGG-D-nucNLS-UGI-SV40-E 3045 bp DNA circular UNA 21-APR-2023 DEFINITION . ACCESSION urn.local...9u-en4mbcm VERSION urn.local...9u-en4mbcm KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="D" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; D" /label="D" CDS 14..367 /codon_start=1 /Name="Synthetic coding sequence of the cytidine deaminase base editor, 3rd generation gene (Zong Y. et al., 2017)" /Clone_Manager_'Draw_As'="Gene" /modified_by="thjac" /label="nucNLS-UGI-SV40" NLS 14..61 /created_by="warddecaestecker" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on protein similarity" /Transferred_From="NLS Nucleoplasmin" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Nucleoplasmin (NLS Nucleoplasmin)" /label="Nucleoplasmin" NLS 344..364 /created_by="warddecaestecker" /modified_by="wacae" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on protein similarity" /Transferred_From="NLS SV40" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /label="SV40" GG complement(371..381) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="E" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; E" /label="E" primer complement(401..421) /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 901..1005 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 1006..1866 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 2037..2625 /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 2989..3010 /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcatca ggtaagaggc cggcggccac caagaaggcc ggccaagcca agaagaagaa 61 gacaagagat tcaggaggat ccaccaacct ctccgacatc atcgagaagg agactggcaa 121 gcagctggtg atccaggaga gcatcttgat gctgcctgag gaggtggagg aggtcatcgg 181 caacaagccg gagtccgaca tcctcgtcca caccgcctac gacgagtcca ccgacgagaa 241 cgtcatgctc ctcacctcag atgctccaga gtacaagccc tgggccttgg tgatccaaga 301 ctccaacggc gagaacaaga tcaagatgct ctccggcggc agccccaaga agaagaggaa 361 ggtttagtaa ctgctgagac cgaattctcg ccctatagtg agtcgtatta cctggccgtc 421 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 481 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 541 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg 601 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag 661 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc 721 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt 781 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag 841 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg 901 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 961 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 1021 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 1081 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 1141 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 1201 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 1261 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 1321 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 1381 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 1441 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 1501 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 1561 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 1621 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 1681 ggctggttta ttgctgataa atctggagcc ggtgagcgtg gctctcgagg tatcattgca 1741 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 1801 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 1861 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 1921 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 1981 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 2041 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 2101 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 2161 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 2221 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 2281 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 2341 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 2401 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 2461 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 2521 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2581 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2641 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2701 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2761 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 2821 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 2881 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 2941 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 3001 caatttcaca cgagctattt aggtgacact atagaagtga agctt //