LOCUS pDE-Cas9(VQR)-Km 15584 bp ds-DNA circular 17-APR-2023 DEFINITION . ACCESSION urn.local...12-ehucfj9 KEYWORDS . SOURCE synthetic DNA construct (unknown) ORGANISM synthetic DNA construct. REFERENCE 1 (bases 1 to 15584) AUTHORS Anne Britt TITLE Direct Submission JOURNAL Exported 15 sep. 2015 from SnapGene 2.8.2 to Vector NTI(R) format http://www.snapgene.com COMMENT VNTDATE|706665600|. FEATURES Location/Qualifiers T-DNA_border 1..333 /modified_by="wacae" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="LB" /Transferred_Similarity="99.70%" /Primary_Match="99.70%; Adjusted-Interval-0:0/0; LB" /label="LB" primer 516..537 /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" promoter 569..1540 /vntifkey=29 /modified_by="wacae" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pPcUBI" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pPcUBI" /label="pPcUBI" CDS 1555..5694 /modified_by="mamee" /label="Cas9" NLS 5671..5691 /created_by="warddecaestecker" /modified_by="wacae" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on protein similarity" /Transferred_From="NLS SV40" /Transferred_Translation_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)" /label="SV40" terminator 5709..6177 /modified_by="mamee" /label="pea 3A term" attR1 6204..6327 /modified_by="mamee" promoter 6352..6382 /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="lacUV5" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="14 CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (14 CmR)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (CmR)" /label="lacUV5" misc_feature 6436..7094 /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="14 CmR" /Transferred_Similarity="99.85%" /Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR (14 CmR)" /Alternative_Match="99.85%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" UTR 7117..7435 /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" CDS 7436..7741 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /label="ccdB" attR2 complement(7782..7905) /modified_by="mamee" Plant_Selection 7970..9269 /modified_by="mamee" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Kanamycin" /Transferred_Similarity="95.75%" /Primary_Match="95.75%; Adjusted-Interval-0:0/0; Kana (Kanamycin)" /label="Kana" promoter 7970..8149 /note="nopaline synthase promoter" /modified_by="mamee" /label="pNOS" CDS 8183..8977 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /modified_by="mamee" /label="NptII" terminator 9017..9269 /note="nopaline synthase terminator and poly(A) signal" /modified_by="mamee" /label="tNOS" T-DNA_border 9270..9456 /modified_by="wacae" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="RB" /Transferred_Similarity="76.62%" /Primary_Match="76.62%; Adjusted-Interval-0:0/0; RB" /label="RB" regulatory 10612..11241 /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" regulatory 11670..12742 /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" rep_origin 12808..13002 /note="origin of replication from Pseudomonas pVS1 plasmid" /modified_by="mamee" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 oriV" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV" /label="pVS1 oriV" regulatory 13376..13636 /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" rep_origin complement(13672..14260) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.83%" /Primary_Match="99.83%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" misc_feature complement(14504..15295) /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" Promoter complement(15296..15577) /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="96.81%" /Primary_Match="96.81%; Adjusted-Interval-0:0/0; P1 (SpR)" /label="P1" ORIGIN 1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc 61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga 121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc 181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg 241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga 301 cgtttttaat gtactgaatt aacgccgaat tgctctagcc aatacgcaaa ccgcctctcc 361 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 421 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 481 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 541 gaaacagcta tgacatgatt acgaattcaa aaattacgga tatgaatata ggcatatccg 601 tatccgaatt atccgtttga cagctagcaa cgattgtaca attgcttctt taaaaaagga 661 agaaagaaag aaagaaaaga atcaacatca gcgttaacaa acggccccgt tacggcccaa 721 acggtcatat agagtaacgg cgttaagcgt tgaaagactc ctatcgaaat acgtaaccgc 781 aaacgtgtca tagtcagatc ccctcttcct tcaccgcctc aaacacaaaa ataatcttct 841 acagcctata tatacaaccc ccccttctat ctctcctttc tcacaattca tcatctttct 901 ttctctaccc ccaattttaa gaaatcctct cttctcctct tcattttcaa ggtaaatctc 961 tctctctctc tctctctctg ttattccttg ttttaattag gtatgtatta ttgctagttt 1021 gttaatctgc ttatcttatg tatgccttat gtgaatatct ttatcttgtt catctcatcc 1081 gtttagaagc tataaatttg ttgatttgac tgtgtatcta cacgtggtta tgtttatatc 1141 taatcagata tgaatttctt catattgttg cgtttgtgtg taccaatccg aaatcgttga 1201 tttttttcat ttaatcgtgt agctaattgt acgtatacat atggatctac gtatcaattg 1261 ttcatctgtt tgtgtttgta tgtatacaga tctgaaaaca tcacttctct catctgattg 1321 tgttgttaca tacatagata tagatctgtt atatcatttt ttttattaat tgtgtatata 1381 tatatgtgca tagatctgga ttacatgatt gtgattattt acatgatttt gttatttacg 1441 tatgtatata tgtagatctg gactttttgg agttgttgac ttgattgtat ttgtgtgtgt 1501 atatgtgtgt tctgatcttg atatgttatg tatgtgcagc gaattcggcg cgccatggat 1561 aagaagtact ctatcggact cgatatcgga actaactctg tgggatgggc tgtgatcacc 1621 gatgagtaca aggtgccatc taagaagttc aaggttctcg gaaacaccga taggcactct 1681 atcaagaaaa accttatcgg tgctctcctc ttcgattctg gtgaaactgc tgaggctacc 1741 agactcaaga gaaccgctag aagaaggtac accagaagaa agaacaggat ctgctacctc 1801 caagagatct tctctaacga gatggctaaa gtggatgatt cattcttcca caggctcgaa 1861 gagtcattcc tcgtggaaga agataagaag cacgagaggc accctatctt cggaaacatc 1921 gttgatgagg tggcatacca cgagaagtac cctactatct accacctcag aaagaagctc 1981 gttgattcta ctgataaggc tgatctcagg ctcatctacc tcgctctcgc tcacatgatc 2041 aagttcagag gacacttcct catcgagggt gatctcaacc ctgataactc tgatgtggat 2101 aagttgttca tccagctcgt gcagacctac aaccagcttt tcgaagagaa ccctatcaac 2161 gcttcaggtg tggatgctaa ggctatcctc tctgctaggc tctctaagtc aagaaggctt 2221 gagaacctca ttgctcagct ccctggtgag aagaagaacg gacttttcgg aaacttgatc 2281 gctctctctc tcggactcac ccctaacttc aagtctaact tcgatctcgc tgaggatgca 2341 aagctccagc tctcaaagga tacctacgat gatgatctcg ataacctcct cgctcagatc 2401 ggagatcagt acgctgattt gttcctcgct gctaagaacc tctctgatgc tatcctcctc 2461 agtgatatcc tcagagtgaa caccgagatc accaaggctc cactctcagc ttctatgatc 2521 aagagatacg atgagcacca ccaggatctc acacttctca aggctcttgt tagacagcag 2581 ctcccagaga agtacaaaga gattttcttc gatcagtcta agaacggata cgctggttac 2641 atcgatggtg gtgcatctca agaagagttc tacaagttca tcaagcctat cctcgagaag 2701 atggatggaa ccgaggaact cctcgtgaag ctcaatagag aggatcttct cagaaagcag 2761 aggaccttcg ataacggatc tatccctcat cagatccacc tcggagagtt gcacgctatc 2821 cttagaaggc aagaggattt ctacccattc ctcaaggata acagggaaaa gattgagaag 2881 attctcacct tcagaatccc ttactacgtg ggacctctcg ctagaggaaa ctcaagattc 2941 gcttggatga ccagaaagtc tgaggaaacc atcacccctt ggaacttcga agaggtggtg 3001 gataagggtg ctagtgctca gtctttcatc gagaggatga ccaacttcga taagaacctt 3061 ccaaacgaga aggtgctccc taagcactct ttgctctacg agtacttcac cgtgtacaac 3121 gagttgacca aggttaagta cgtgaccgag ggaatgagga agcctgcttt tttgtcaggt 3181 gagcaaaaga aggctatcgt tgatctcttg ttcaagacca acagaaaggt gaccgtgaag 3241 cagctcaaag aggattactt caagaaaatc gagtgcttcg attcagttga gatttctggt 3301 gttgaggata ggttcaacgc atctctcgga acctaccacg atctcctcaa gatcattaag 3361 gataaggatt tcttggataa cgaggaaaac gaggatatct tggaggatat cgttcttacc 3421 ctcaccctct ttgaagatag agagatgatt gaagaaaggc tcaagaccta cgctcatctc 3481 ttcgatgata aggtgatgaa gcagttgaag agaagaagat acactggttg gggaaggctc 3541 tcaagaaagc tcattaacgg aatcagggat aagcagtctg gaaagacaat ccttgatttc 3601 ctcaagtctg atggattcgc taacagaaac ttcatgcagc tcatccacga tgattctctc 3661 acctttaaag aggatatcca gaaggctcag gtttcaggac agggtgatag tctccatgag 3721 catatcgcta acctcgctgg atctcctgca atcaagaagg gaatcctcca gactgtgaag 3781 gttgtggatg agttggtgaa ggtgatggga aggcataagc ctgagaacat cgtgatcgaa 3841 atggctagag agaaccagac cactcagaag ggacagaaga actctaggga aaggatgaag 3901 aggatcgagg aaggtatcaa agagcttgga tctcagatcc tcaaagagca ccctgttgag 3961 aacactcagc tccagaatga gaagctctac ctctactacc tccagaacgg aagggatatg 4021 tatgtggatc aagagttgga tatcaacagg ctctctgatt acgatgttga tcatatcgtg 4081 ccacagtcat tcttgaagga tgattctatc gataacaagg tgctcaccag gtctgataag 4141 aacaggggta agagtgataa cgtgccaagt gaagaggttg tgaagaaaat gaagaactat 4201 tggaggcagc tcctcaacgc taagctcatc actcagagaa agttcgataa cttgactaag 4261 gctgagaggg gaggactctc tgaattggat aaggcaggat tcatcaagag gcagcttgtg 4321 gaaaccaggc agatcactaa gcacgttgca cagatcctcg attctaggat gaacaccaag 4381 tacgatgaga acgataagtt gatcagggaa gtgaaggtta tcaccctcaa gtcaaagctc 4441 gtgtctgatt tcagaaagga tttccaattc tacaaggtga gggaaatcaa caactaccac 4501 cacgctcacg atgcttacct taacgctgtt gttggaaccg ctctcatcaa gaagtatcct 4561 aagctcgagt cagagttcgt gtacggtgat tacaaggtgt acgatgtgag gaagatgatc 4621 gctaagtctg agcaagagat cggaaaggct accgctaagt atttcttcta ctctaacatc 4681 atgaatttct tcaagaccga gattaccctc gctaacggtg agatcagaaa gaggccactc 4741 atcgagacaa acggtgaaac aggtgagatc gtgtgggata agggaaggga tttcgctacc 4801 gttagaaagg tgctctctat gccacaggtg aacatcgtta agaaaaccga ggtgcagacc 4861 ggtggattct ctaaagagtc tatcctccct aagaggaact ctgataagct cattgctagg 4921 aagaaggatt gggaccctaa gaaatacggt ggtttcgtgt ctcctaccgt ggcttactct 4981 gttctcgttg tggctaaggt tgagaaggga aagagtaaga agctcaagtc tgttaaggaa 5041 cttctcggaa tcactatcat ggaaaggtca tctttcgaga agaacccaat cgatttcctc 5101 gaggctaagg gatacaaaga ggttaagaag gatctcatca tcaagctccc aaagtactca 5161 ctcttcgaac tcgagaacgg tagaaagagg atgctcgctt ctgctggtga gcttcaaaag 5221 ggaaacgagc ttgctctccc atctaagtac gttaactttc tttacctcgc ttctcactac 5281 gagaagttga agggatctcc agaagataac gagcagaagc aacttttcgt tgagcagcac 5341 aagcactact tggatgagat catcgagcag atctctgagt tctctaaaag ggtgatcctc 5401 gctgatgcaa acctcgataa ggtgttgtct gcttacaaca agcacagaga taagcctatc 5461 agggaacagg cagagaacat catccatctc ttcaccctta ccaacctcgg tgctcctgct 5521 gctttcaagt acttcgatac aaccatcgat aggaagcagt acagatctac caaagaagtg 5581 ctcgatgcta ccctcatcca tcagtctatc actggactct acgagactag gatcgatctc 5641 tcacagctcg gtggtgattc aagggctgat cctaagaaga agaggaaggt ttgaggcgcg 5701 ccgagctcca ggcctcccag ctttcgtccg tatcatcggt ttcgacaacg ttcgtcaagt 5761 tcaatgcatc agtttcattg cccacacacc agaatcctac taagtttgag tattatggca 5821 ttggaaaagc tgttttcttc tatcatttgt tctgcttgta atttactgtg ttctttcagt 5881 ttttgttttc ggacatcaaa atgcaaatgg atggataaga gttaataaat gatatggtcc 5941 ttttgttcat tctcaaatta ttattatctg ttgtttttac tttaatgggt tgaatttaag 6001 taagaaagga actaacagtg tgatattaag gtgcaatgtt agacatataa aacagtcttt 6061 cacctctctt tggttatgtc ttgaattggt ttgtttcttc acttatctgt gtaatcaagt 6121 ttactatgag tctatgatca agtaattatg caatcaagtt aagtacagta taggcttgag 6181 ctccctaggc ctgttatccc taacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa 6241 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg 6301 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt 6361 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga 6421 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa 6481 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag 6541 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt 6601 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg 6661 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc 6721 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag 6781 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct 6841 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt 6901 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa 6961 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt 7021 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg 7081 caggcggggc gtaatctaga ggatccggct tactaaaagc cagataacag tatgcgtatt 7141 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa 7201 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt 7261 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa 7321 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc 7381 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca 7441 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag 7501 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct 7561 gtcagataaa gtcccccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg 7621 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga 7681 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata 7741 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt 7801 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat 7861 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttcga taattccgat 7921 ccagcctagg cccgggcctg aggacgcgtc catggttaat taagacgtcg aaccgcaacg 7981 ttgaaggagc cactcagccg cgggtttctg gagtttaatg agctaagcac atacgtcaga 8041 aaccattatt gcgcgttcaa aagtcgccta aggtcactat cagctagcaa atatttcttg 8101 tcaaaaatgc tccactgacg ttccataaat tcccctcggt atccaattag agtctcatat 8161 tcactctcaa ctcgatcgag gcatgattga acaagatgga ttgcacgcag gttctccggc 8221 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 8281 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 8341 gtccggtgcc ctgaatgaac tccaagacga ggcagcgcgg ctatcgtggc tggccacgac 8401 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 8461 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 8521 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 8581 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 8641 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 8701 gctcaaggcg cggatgcccg acggcgagga tctcgtcgtg acccacggcg atgcctgctt 8761 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 8821 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 8881 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 8941 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcggactc 9001 tagctagagt caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc 9061 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat 9121 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca 9181 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc 9241 gcgcgcggtg tcatctatgt tactagatcg tttaaactat cagtgtttga caggatatat 9301 tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt 9361 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg 9421 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag 9481 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg 9541 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt 9601 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct 9661 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg 9721 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc 9781 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc 9841 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg 9901 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg 9961 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc 10021 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca 10081 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag 10141 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg 10201 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg 10261 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca 10321 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc 10381 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct 10441 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac 10501 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt 10561 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt 10621 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc 10681 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt 10741 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc 10801 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga 10861 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt 10921 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag 10981 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc 11041 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt 11101 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc 11161 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa 11221 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca 11281 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg 11341 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc 11401 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg 11461 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa 11521 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt 11581 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc 11641 actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca 11701 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca 11761 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg 11821 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc 11881 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga 11941 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg 12001 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg 12061 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc 12121 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt 12181 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt 12241 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa 12301 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt 12361 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg 12421 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat 12481 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga 12541 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa 12601 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa 12661 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg 12721 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg 12781 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc 12841 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc 12901 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt 12961 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac 13021 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc 13081 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa 13141 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg 13201 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 13261 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 13321 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag 13381 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 13441 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc 13501 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 13561 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 13621 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 13681 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 13741 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 13801 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 13861 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 13921 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 13981 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 14041 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 14101 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 14161 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 14221 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 14281 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga 14341 tatatctccc aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt 14401 gacctgatag tttggctgtg agcaattatg tgcttagtgc atctaatcgc ttgagttaac 14461 gccggcgaag cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct 14521 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca 14581 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact 14641 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg 14701 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc 14761 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt 14821 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc 14881 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg 14941 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc 15001 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct 15061 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat 15121 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat 15181 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga 15241 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt 15301 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca 15361 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc 15421 caaaaaaaca tgtcataaca agaagccatg aaaaccgcca ctgcgccgtt accaccgctg 15481 cgttcggtca aggttctgga ccagttgcgt gacggcagtt acgctacttg cattacagct 15541 tacgaaccga acgaggctta tgtccactgg gttcgtgccc gaat //