LOCUS       pB7m34GW-p35S-SACL3-eGFP       10939 bp    DNA     circular UNA 13-APR-2023
DEFINITION  .
ACCESSION   urn.local...1l-ddops7t
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     Promoter        8..289
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="96.81%"
                     /Primary_Match="96.81%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    290..1081
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1325..1913
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     regulatory      complement(1949..2209)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150;
                     <a href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      2583..2777
                     /note="origin of replication from Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2843..3915)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     regulatory      complement(4344..4973)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     T-DNA_border    6159..6358
                     /vntifkey=87
                     /modified_by="mamee"
                     /label="RB"
     primer          6385..6405
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="95.24%"
                     /Primary_Match="95.24%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     Gateway_flank   6422..6442
                     /label="attB4"
     promoter        6445..7567
                     /vntifkey=29
                     /note="35S Cauliflower mosaic virus promotor"
                     /modified_by="mamee"
                     /label="p35S"
     Gateway_flank   7569..7589
                     /label="attB1"
     CDS             7595..8350
                     /vntifkey=21
                     /modified_by="mamee"
                     /label="SACL3"
     Gateway_flank   complement(8355..8375)
                     /modified_by="mamee"
                     /label="attB2"
     CDS             8378..9097
                     /vntifkey=61
                     /label="EGFP"
     Gateway_flank   complement(9099..9119)
                     /label="attB3"
     terminator      9187..9426
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="35ST"
                     /Transferred_Similarity="95.42%"
                     /Primary_Match="95.42%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1d-c4nnd54"">35ST</a>"
                     /modified_by="mamee"
                     /label="t35S"
     promoter        9466..9738
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:ip-f0oyhrv"">pNOS v2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /Alternative_Match="99.65%; Adjusted-Interval-0:-10/0;
                     pNOS (<a href=""urn:local:.:fi-eaul7he"">2
                     Hygromycin</a>)"
                     /Alternative_Match="99.64%; Adjusted-Interval-0:-6/0; pNOS
                     (<a href=""urn:local:.:6g-f1tbabl"">pNOS v1</a>)"
                     /label="pNOS"
     Plant_Selection 9466..10586
                     /Color="#006600"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="99.82%"
                     /Primary_Match="99.82%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1k-e500sax"">Basta</a>"
                     /Alternative_Match="98.59%; Adjusted-Interval-0:-15/0;
                     Basta (<a href=""urn:local:.:fe-eaul7he"">1 Basta</a>)"
                     /label="Basta"
     CDS             9759..10310
                     /%_Identity=100
                     /Motif="ATGAGCCCAGAACGACGCCCGGCCGACATCCGCCGTGCCACCGAGGCGGA
                     CATGCCGGCGGTCTGCACCATCGTCAACCACTACATCGAGACAAGCACGGTCAACTTC
                     CGTACCGAGCCGCAGGAACCGCAGGAGTGGACGGACGACCTCGTCCGTCTGCGGGAGC
                     GCTATCCCTGGCTCGTCGCCGAGGTGGACGGCGAGGTCGCCGGCATCGCCTACGCGGG
                     TCCCTGGAAGGCACGCAACGCCTACGACTGGACGGCCGAGTCGACCGTGTACGTCTCC
                     CCCCGCCACCAGCGGACGGGACTGGGCTCCACGCTCTACACCCACCTGCTGAAGTCCC
                     TGGAGGCACAGGGCTTCAAGAGCGTGGTCGCTGTCATCGGGCTGCCCAACGACCCGAG
                     CGTGCGCATGCACGAGGCGCTCGGATATGCCCCCCGCGGCATGCTGCGGGCGGCCGGC
                     TTCAAGCACGGGAACTGGCATGACGTGGGTTTCTGGCAGCTGGACTTCAGCCTGCCGG
                     TGCCGCCCCGTCCGGTCCTGCCCGTCACCGAAATCTGA"
                     /annotation_group="PAT: 8,732 -> 9,283"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PAT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:3h-c4nnd56"">PAT</a>"
                     /label="PAT"
     terminator      10317..10586
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:6c-dgycryw"">NOST</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2 Hygromycin"
                     /Transferred_Similarity="99.26%"
                     /Primary_Match="99.26%; Adjusted-Interval-0:0/0; tNOS (<a
                     href=""urn:local:.:fi-eaul7he"">2 Hygromycin</a>)"
                     /Alternative_Match="99.26%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="99.26%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:fj-eaul7he"">3 Kanamycin</a>)"
                     /Alternative_Match="99.26%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:fe-eaul7he"">1 Basta</a>)"
                     /Alternative_Match="99.26%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2u-f1y9293"">tNOS v2</a>)"
                     /Alternative_Match="99.26%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="99.26%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /Alternative_Match="99.22%; Adjusted-Interval-0:13/-2;
                     tNOS (<a href=""urn:local:.:38-f1t9off"">tNOS v1</a>)"
                     /label="tNOS"
     T-DNA_border    10607..10939
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1e-c4nnd54"">LB</a>"
                     /label="LB"
ORIGIN      
        1 attcgggcac gaacccagtg gacataagcc tcgttcggtt cgtaagctgt aatgcaagta
       61 gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      121 gcagtggcgg ttttcatggc ttcttgttat gacatgtttt tttggggtac agtctatgcc
      181 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
      241 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
      301 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
      361 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
      421 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
      481 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
      541 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
      601 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
      661 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
      721 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
      781 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
      841 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
      901 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
      961 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     1021 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     1081 atgtctagct agaaattcgt tcaagccgac gccgcttcgc cggcgttaac tcaagcgatt
     1141 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
     1201 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
     1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1441 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     2161 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     2221 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2281 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2341 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     2401 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     2461 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     2521 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     2581 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     2641 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     2701 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     2761 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
     2821 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     2881 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     2941 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     3001 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     3061 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     3121 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     3181 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     3241 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     3301 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     3361 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     3421 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     3481 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     3541 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     3601 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     3661 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     3721 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     3781 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     3841 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     3901 gtttgcgacc gtcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     3961 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     4021 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     4081 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     4141 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     4201 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     4261 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     4321 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     4381 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     4441 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     4501 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     4561 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     4621 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
     4681 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
     4741 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
     4801 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
     4861 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
     4921 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
     4981 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
     5041 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
     5101 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
     5161 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
     5221 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
     5281 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
     5341 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
     5401 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
     5461 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
     5521 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
     5581 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
     5641 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
     5701 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
     5761 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
     5821 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
     5881 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
     5941 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
     6001 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
     6061 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
     6121 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
     6181 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
     6241 ccgttattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
     6301 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctaagcttg
     6361 ggcccgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcta
     6421 tcaactttgt atagaaaagt tgtcatttag gtgacactat agaatactca agctatgcat
     6481 ccaacgcgtt gggagctctc ccatatggtc gactagagcc aagctgatct cctttgcccc
     6541 ggagatcacc atggacgact ttctctatct ctacgatcta ggaagaaagt tcgacggaga
     6601 aggtgacgat accatgttca ccaccgataa tgagaagatt agcctcttca atttcagaaa
     6661 gaatgctgac ccacagatgg ttagagaggc ctacgcggca ggtctcatca agacgatcta
     6721 cccgagtaat aatctccagg agatcaaata ccttcccaag aaggttaaag atgcagtcaa
     6781 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     6841 tactattcca gtatggacga ttcaaggctt gcttcataaa ccaaggcaag taatagagat
     6901 tggagtctct aagaaagtag ttcctactga atcaaaggcc atggagtcaa aaattcagat
     6961 cgaggatcta acagaactcg ccgtgaagac tggcgaacag ttcatacaga gtcttttacg
     7021 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc tcgtctactc
     7081 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
     7141 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
     7201 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
     7261 cgttcaagat gcccctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     7321 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     7381 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     7441 aggaagttca tttcatttgg agaggactcc ggtattttta caacaattac cacaacaaaa
     7501 caaacaacaa acaacattac aatttactat tctagtcgac ctgcaggcgg ccgcactagt
     7561 gatatcagca agtttgtaca aaaaagcagg caccatgcag aacaatcagt ttcctcactt
     7621 ctcagatgaa gtgggtgaca gaaatatgca caacccgtat gcatcagggt catcctatga
     7681 tgctttgttc ccaccatgtg caaagttgcc ataccatggt gttgaacttc aaccgtctgc
     7741 ggtctgtcca aagaactttg tcatcttcga tcaaacatat gaccgcagcc aagtgatgta
     7801 ccatcctgag ctgactcata agctcatgaa taccccttcg ttgaacaatt tagcttcgac
     7861 gtttcagaac gagtatgttg ggggaagtta tggtaactat ggtaactatg agcaagaagt
     7921 atcctcttct tatcaagaag atccaaatga gatcgatgct ctcttgagcg cagatgaaga
     7981 ttatgaagag aatgatgata atgaaggtga agaggatggt ggtgattcag aagaagtcag
     8041 cactgctcgt acttcttcca gggattatgg aaacaccaca gcagaatctt gttgttccag
     8101 ttatggttac aacaacaaca acaacaacaa ctcaaggaag cagagtttat cgggcagtgc
     8161 tagtagtagt aacaatgatg ggaaaggacg taaaaagatg aagaagatga tgggagtatt
     8221 gaggagaatt gtccctggag gagaacagat gaatacagct tgcgttcttg atgaagctgt
     8281 tcagtatctc aagtcactta aaatcgaagc tcagaaactt ggcgttggac atttctcaaa
     8341 ccaatcttga ttgccagctt tcttgtacaa agtggggatg gtgagcaagg gcgaggagct
     8401 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     8461 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     8521 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     8581 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     8641 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     8701 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     8761 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     8821 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     8881 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     8941 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     9001 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     9061 cgggatcact ctcggcatgg acgagctgta caagtaagca actttattat acatagttga
     9121 taattcactg gccgtcgttt tacaacgtcg tgactgggaa aacgatctaa ctgactagcc
     9181 gcggccatgg cggccgggag cggccatgct agagtccgca aaaatcacca gtctctctct
     9241 acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg
     9301 aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
     9361 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
     9421 tgacctgcag gtcgaccata tgggagagct cgaattatca tacatgagaa ttaagggagt
     9481 cacgttatga cccccgccga tgacgcggga caagccgttt tacgtttgga actgacagaa
     9541 ccgcaacgtt gaaggagcca ctgagccgcg ggtttctgga gtttaatgag ctaagcacat
     9601 acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat
     9661 atttcttgtc aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag
     9721 tctcatattc actctcaact cgatcgaggg gatctaccat gagcccagaa cgacgcccgg
     9781 ccgacatccg ccgtgccacc gaggcggaca tgccggcggt ctgcaccatc gtcaaccact
     9841 acatcgagac aagcacggtc aacttccgta ccgagccgca ggaaccgcag gagtggacgg
     9901 acgacctcgt ccgtctgcgg gagcgctatc cctggctcgt cgccgaggtg gacggcgagg
     9961 tcgccggcat cgcctacgcg ggtccctgga aggcacgcaa cgcctacgac tggacggccg
    10021 agtcgaccgt gtacgtctcc ccccgccacc agcggacggg actgggctcc acgctctaca
    10081 cccacctgct gaagtccctg gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc
    10141 tgcccaacga cccgagcgtg cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc
    10201 tgcgggcggc cggcttcaag cacgggaact ggcatgacgt gggtttctgg cagctggact
    10261 tcagcctgcc ggtgccgccc cgtccggtcc tgcccgtcac cgaaatctga tgacccctag
    10321 agtcaagcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
    10381 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
    10441 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
    10501 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
    10561 gtgtcatcta tatgttacta gatcgaccgg catgcaagct gataattcaa ttcggcgtta
    10621 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
    10681 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
    10741 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
    10801 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
    10861 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
    10921 gcgttgctgc ctgtgatca
//