LOCUS pGG-pUM140_0016-UM119_0052.1_Vacuole-YFP-NOST-PIBT3 8378 bp DNA circular UNA 12-APR-2023 DEFINITION . ACCESSION urn.local.jonasblomme.1g-cgtoeil VERSION urn.local.jonasblomme.1g-cgtoeil KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 2..1935 /modified_by="jonasblomme" /label="pUM140_0016" CDS join(1970..1984,2068..2154,2240..2340,2434..2587, 2656..2864,3054..3174) /note /label="UM119_0052.1" intron 1985..2067 /created_by="jonasblomme" /label="Intron" intron 2155..2239 /created_by="jonasblomme" /label="Intron" intron 2341..2433 /created_by="jonasblomme" /label="Intron" intron 2588..2655 /created_by="jonasblomme" /label="Intron" intron 2865..3053 /created_by="jonasblomme" /label="Intron" CDS 3181..3900 /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /modified_by="jonasblomme" /label="YFP" terminator 3905..4172 /vntifkey=43 /modified_by="mamee" /label="tNOS" Linker 4177..4225 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker II" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II" /label="Linker II" Plant_Selection 4238..6064 /created_by="mamee" /modified_by="mamee" /label="PIBT3" promoter 4238..4783 /label="UmpRbcS" CDS join(4784..4791,5289..5655) /modified_by="jonasblomme" /label="ble" intron 4792..5288 /gene="ble" /note="Ulva mutabilis RbcS intron 1; enhancer" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="RbcsI" /Transferred_Similarity="99.40%" /Primary_Match="99.40%; Adjusted-Interval-0:0/0; RbcsI" /modified_by="jonasblomme" /label="RbcsI" terminator 5656..6064 /label="Ulva trbcS" primer complement(6069..6089) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 6150..6200 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 6201..7010 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 7157..7745 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 8344..8364 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca 61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg 121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg 181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag 241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca 301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt 361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt 421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct 481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta 541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt 601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt 661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag 721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat 781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg 841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg 901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg 961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag 1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga 1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg 1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg 1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg 1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct 1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag 1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc 1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt 1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat 1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc 1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg 1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa 1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt 1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc 1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc 1921 tatcagtgat taacaaacag tattcagtcg actggtacca acaggctcca tgaacgagag 1981 tgaggtgcgc tttgacatgt cgtaccggcc gagtgaagcg tctggtgcat aacgcgatgg 2041 aggccttttg aaccaccgat cctgtaggtc aacagacaga ttgaacagat ggtgatgttc 2101 atcaagcagg aagctgagga aaaagccaac gaaatccgca tgtctgctga ggaggcgcgt 2161 cacccgcttg tccagaatgc cttttgttct gatgtttcaa caaattaact cagcggccga 2221 ttaaaatgca tgcatgcagg aatttaatct ggaaaagctc aacctgctcg aaactgaaaa 2281 gcagcgcatc cgcaaagaat acgagcgcaa ggaggcgcag gtggaggcca acaaaaagat 2341 gtgcgcagtc aaatttaagt gtgcgtgtga gccggaaaaa tatggcattg cgtacctcat 2401 atctcagcgt tgacccgacc gactgtgccg cagtgaggcc tcgaagcagc ataatgaaca 2461 acggctgaaa gtgcttggtg caaagcagcg cttgatcaaa gacgtgttac aggctgcaaa 2521 ggatgagttg aagaagaaag ctggtggtag tggttacgct gatctcgtga gcgacctttt 2581 ggtgcaggta tgcttcgctg tttgcgtgcg ctgtgacttg taacatcgct ctcaaatgca 2641 cgtgactata tttaggcgat gtacaaactt caagagccaa gccagatcgt tcagtgccgg 2701 gaggcagatc tgaaggtcat agagtcatgt gtgccaaagg cacaggagaa gtataaaagt 2761 gtatatggca gtgatgcacc gcagctcacc attgatcgca agcactttct tcccgcagct 2821 gcaacatctc aggaacaaga ggatgacccg gatgcgccta cttggtgcgg cagctgaatt 2881 tgtgatcagc tcgggtgata agttgtttgt gatagctctc tttgcacagt ttgtgttttt 2941 ctgctgcatg tcgctctctc atttaccatt ttacctgagg gacactttct gactacctgc 3001 ggacttggtc tactaacaat tacattctgc taatttgagt tgagtttgtg cagcctggga 3061 ggtgttgtgg tgacctctat tagtggccgc attgttgtcg ataacacact ggacgccagg 3121 ctcaaaatcg tcgaggacaa gcagcttcca gcgatcagag ctcaattgtt cgagtcagct 3181 atggtgagca agggcgagga gctgttcacg ggcgtggtgc ccatcctggt ggagctggac 3241 ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga cgccacctac 3301 ggcaagctga ccctgaagtt catctgcacg accggcaagc tgcccgtgcc ctggcccacc 3361 ctggtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag 3421 cagcacgact tcttcaagag cgccatgccg gagggctacg tgcaggagcg caccatcttc 3481 ttcaaggacg acggcaacta caagacccgc gcggaggtga agttcgaggg cgacacgctg 3541 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctgggccac 3601 aagctggagt acaactacaa cagccacaac gtgtacatca tggccgacaa gcagaagaac 3661 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggctcggt gcagctggcg 3721 gaccactacc agcagaacac ccccatcggc gacggcccgg tgctgctgcc cgacaaccac 3781 tacctgtcct accagagcgc cctgtccaag gaccccaacg agaagcgcga ccacatggtg 3841 ctgctggagt tcgtgaccgc cgccggcatc accctgggca tggacgagct gtacaagtga 3901 ctgcctagag tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc 3961 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa 4021 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc 4081 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat 4141 cgcgcgcggt gtcatctatg ttactagatc gaactataag ctaggtagta actagtgcga 4201 ctgtctagaa tcgcgtatgc gagtgtatga gctcgatcgt tgttcgtaca caaggatgtc 4261 gcttgtggct ttttcgctcg gctttccagg agggcagtcc agataccatg cggccgccgt 4321 gtccatcact cgcgctgcag ctgatgtggg cagatactgc actaatctgc cgcacgacgc 4381 gtcggcctga gtcggcggac accggaggct tgctggagta ctggcacaag tgtgcgtgtg 4441 attgccagaa acagcaaccc actcactaga tataccgctc caataatggg ttcatgtgca 4501 agctcactca ataacgaaac gcttcactat tgtgcacgtg ccggggttgc ttgggtatgg 4561 cgtgccatac gggttctgca catgtcaccc acgtgggtgt ggcacgagat atcgaggtgg 4621 ccatgtaaaa ggcatttgct agagggcagc tcgtccatgc cgctcgagca caccgagtgc 4681 gcaccataaa tgcagcacat gcctgtgcag cctatctcct gtctctgtta catcccacca 4741 cctcctcaca tatccccttc cacccttaaa ccgcacagca gacatggcca agtgcgcatc 4801 gcgtgtcaat gctggcgctg ctgacgtccg ctgcctaaac tgcatgaaag ctcctgcagt 4861 cgcaagtagt ccttttaact tgttgtatcg gaacaaatgg ttgtagtctg tgccgtgtga 4921 cgatcttgag ccgcctggtc ggcaggtcca atgtcggcct ggaccctgca caccctgccc 4981 accctgccgc tgctcacccg ttcacgaccc cacaggtgct cgcagcagta attgtcgata 5041 gatagcaact gtgttctgac tattctttga ccttccgcat ttgggactgc ggcggcacac 5101 ctactatccg caatgcacac cctggtgctg gtactgcctg cctttccgcg cgagcccaca 5161 ccatgcaggc gcatagtaat ggactgtgat tgatttaggc aactgtatga aacctcttca 5221 tcgtgccttg gttgcttgcg ataccctcac gtgagttcat atgaccaacg ctggcacgac 5281 ccctgcagga tcaccagcgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc 5341 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt 5401 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac 5461 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag 5521 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag 5581 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc 5641 gaggagcagg actaagcagt caggcagagt cgttgttggg tgtatggttg cacaaagcta 5701 gacctcgagc tggtcggtta gaaagagatg cttgtgcgac tggatgtaac aggcacaatc 5761 cgaggaaaat gcaacagaaa cgttatgatg taacatctga gcacagtcaa taaaattgca 5821 tgttgtatgc cgcacacaaa agtgttgtta aacatgcgat gtggttgtca aatcattatt 5881 cgcacaacca tggaccgaac ctctggcacg ctgcatacgc tgtattcatt cacatcatgt 5941 tttctacttg tcacatgctc tgcaccaagt aactccgatt agttaggttc gcacatgtca 6001 tcggagtgga ccacttcgaa tcatttggtt cgaagataaa ccggatcccc tatagtgagt 6061 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat 6121 gttacattgc acaagataaa ataatatcat catgaacaat aaaactgtct gcttacataa 6181 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa 6241 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat 6301 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac 6361 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga 6421 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt 6481 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt 6541 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg 6601 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa 6661 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg 6721 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc 6781 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg 6841 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc 6901 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc 6961 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg 7021 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat 7081 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa 7141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 7201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 7261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 7321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 7381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 7441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 7501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 7561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 7621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 7681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 7741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 7801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 7861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 7921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 7981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc 8041 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt 8101 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg 8161 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac 8221 cgacaaacaa cagataaaac gaaaggccca gacttccgac tgagcctttc gttttatttg 8281 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac 8341 gttgtaaaac gacggccagt cttaagctcg ggcccacc //