LOCUS       pGG-pUM140_0016-UM001_0379.1_HistoneH3-YFP-NOST-PIBT3        7719 bp    DNA     circular UNA 12-APR-2023
DEFINITION  .
ACCESSION   urn.local.jonasblomme.17-cgtoeih
VERSION     urn.local.jonasblomme.17-cgtoeih
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        2..1935
                     /modified_by="jonasblomme"
                     /label="pUM140_0016"
     CDS             join(1970..2298,2437..2515)
                     /note
                     /label="UM001_0379.1"
     intron          2299..2436
                     /created_by="jonasblomme"
                     /label="Intron"
     CDS             2522..3241
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /modified_by="jonasblomme"
                     /label="YFP"
     terminator      3246..3513
                     /vntifkey=43
                     /modified_by="mamee"
                     /label="tNOS"
     Linker          3518..3566
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     Plant_Selection 3579..5405
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="PIBT3"
     promoter        3579..4124
                     /label="UmpRbcS"
     CDS             join(4125..4132,4630..4996)
                     /modified_by="jonasblomme"
                     /label="ble"
     intron          4133..4629
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RbcsI"
                     /Transferred_Similarity="99.40%"
                     /Primary_Match="99.40%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:jonasblomme:m-cnspdx5"">RbcsI</a>"
                     /modified_by="jonasblomme"
                     /label="RbcsI"
     terminator      4997..5405
                     /modified_by="mamee"
                     /label="Ulva tRbcS"
     primer          complement(5410..5430)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        5491..5541
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    5542..6351
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      6498..7086
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          7685..7705
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ttgccgaatt cgcagtgaac ccgcatggca gggtggtcac aggtgtcctt tggctgcaca
       61 gtaacatgac cgtcatgcat gggacagagg ttgtgagcag catgcgtcga tgcatacatg
      121 cgcatgtagc atgcagcagt gagctccaga gtggaaagct ggcccgctgt cgtgtgtccg
      181 gaccgacagg ccaggctctg gactgggcca tagtcagcag ggcagaggta ctatgtctag
      241 cgccttcctg ggtgtgcagc tttgccttgc catgcttgga catgaatgag tggggcgcca
      301 ggcacttaca agagccacct cgtggtgtgg gcatatggca gaggggcacc catcagtcgt
      361 gtgccattca gaccgtgacg aagcaatcaa tgactgtgct tgtccgggag aaagtctgtt
      421 gtgaaatttg ctgtgatcta ggggccaatg tggcagtcag gaatggctag agatgctgct
      481 agtgtcgtgc atacctggtg tactgccaac agcctcacct gaccatggtg cttgtgttta
      541 aagcaagcat gccacaagtc ggcatgtagg catacggcaa gagctacaca gttggctgtt
      601 gacctgtgga tgcgtgaaac agaagtttga atcgtgccct ttcgcgttga tgcaccctgt
      661 gtgggagcca ggctggtgtg tgtggaagcc aggctggtgg cacacctgtg tgacctggag
      721 gtggacgacc gggtggcgag gcaagtagca gtttcatcaa agaaacacaa gctgatgcat
      781 ccgcgatagt agcacgattc taatgtgtgc acgcaaacgc atcggcccaa ctggagcagg
      841 ggatgcgcag cagcggccgc tggttccacg aactcgaagc gcaaagagca gtccgctgtg
      901 accagcattc cgctagtgtc agggacgata tgaatacggg ttgggcacat tatgtttggg
      961 tcgaggtcgc catggcggac gtgcaagcgg ctgtcgatca cgcctatcgg agatgcccag
     1021 cgcctgcatc gatatgcaac cagctctgca ccagcgcagc aatacgacag ctgcagccga
     1081 tggcctccta gacactgtgc aacaacaaaa aacaattgga ccgtgacggc acaggtgtcg
     1141 aacacagggg ctgagagagg actgcctgtg cttgtgctcc agtgctcata cgccatactg
     1201 atacgcctcc accgatggcc tcactgcctg cggacactgg cagacgctgt cgtttggatg
     1261 tcgcatcgcc atcttcagtg atgttgtggc gttcggcagg atgtacgcat gagcgattct
     1321 tctaaacgag cagtggtggc acatcagcgg ctccctcaaa ccacaatgcc ggcttggaag
     1381 gtagagcatc tgctcggcaa gccgacatag acgatacagc tagcacagcc ggcttgcagc
     1441 tcatcaatca gtacacatac cgtacgtgtc tgaagtatgc cttgttgata tgaacactgt
     1501 accgtggggc cagacgacac ggtctgaagc accgcctctg cagcatctcg cacgattgat
     1561 gcgcatcaat tgcttgcaat tcgatttgca tttggcgcag ttgtctgcac aatctttgcc
     1621 aacgatcgca tgatccgatt caatgatccg aagcatgatc aatacaccgc attagcagtg
     1681 catgcgcgat tgggacacct ctgggggggg atctgatgca cgttcgacac gggtgccgaa
     1741 cctgcgagaa ggctcactac acgcgtgcgg acgcatgcat gcagtgccaa agatctcgtt
     1801 tgaactgcgc gccagcacat tgcagtgcac gccccgtgcc aggcgaaaac gtatttaacc
     1861 ccgaagccag catactttct ctaccagttc atctcttgtt gatctacctc caccacagtc
     1921 tatcagtgat taacaaacag tattcagtcg actggtacca acaggctcca tggcacgtac
     1981 caagcagacc gcacgtaagt ccactggagg aaaggctcct cgtaagcagc tggccaccaa
     2041 ggcagctcgt aagtctgccc cagcgactgg aggtgtcaag aagcctcatc gctacaggcc
     2101 tggtacagtc gcgctgcgtg agatccgcaa gtaccagaag tctaccgagc tccttatccg
     2161 caagctgcct ttccagcgcc ttgtccgtga gattgctcag gactacaaaa ccgatcttcg
     2221 gttccagagc tcagctgttt tggcgcttca ggaagctgcc gaggcatacc tggtcggcct
     2281 gtttgaggat accaacctgt gcgcagctga cgaccgaatt ccggctgatg atcgtatcag
     2341 cttctatgca ttattttgcg cagttgaacg gcgctcctgt gtgactggtc ggtgttagac
     2401 aagtgcttat gctgacaaag cctaaatctg ctgcaggtgt gcaattcacg caaagagggt
     2461 gacgatcatg cccaaggatg ttcagcttgc ccgccgcatt cgtggtgagc gcgcctcagc
     2521 tatggtgagc aagggcgagg agctgttcac gggcgtggtg cccatcctgg tggagctgga
     2581 cggcgacgtg aacggccaca agttcagcgt gtccggcgag ggcgagggcg acgccaccta
     2641 cggcaagctg accctgaagt tcatctgcac gaccggcaag ctgcccgtgc cctggcccac
     2701 cctggtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
     2761 gcagcacgac ttcttcaaga gcgccatgcc ggagggctac gtgcaggagc gcaccatctt
     2821 cttcaaggac gacggcaact acaagacccg cgcggaggtg aagttcgagg gcgacacgct
     2881 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctgggcca
     2941 caagctggag tacaactaca acagccacaa cgtgtacatc atggccgaca agcagaagaa
     3001 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggctcgg tgcagctggc
     3061 ggaccactac cagcagaaca cccccatcgg cgacggcccg gtgctgctgc ccgacaacca
     3121 ctacctgtcc taccagagcg ccctgtccaa ggaccccaac gagaagcgcg accacatggt
     3181 gctgctggag ttcgtgaccg ccgccggcat caccctgggc atggacgagc tgtacaagtg
     3241 actgcctaga gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     3301 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     3361 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     3421 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     3481 tcgcgcgcgg tgtcatctat gttactagat cgaactataa gctaggtagt aactagtgcg
     3541 actgtctaga atcgcgtatg cgagtgtatg agctcgatcg ttgttcgtac acaaggatgt
     3601 cgcttgtggc tttttcgctc ggctttccag gagggcagtc cagataccat gcggccgccg
     3661 tgtccatcac tcgcgctgca gctgatgtgg gcagatactg cactaatctg ccgcacgacg
     3721 cgtcggcctg agtcggcgga caccggaggc ttgctggagt actggcacaa gtgtgcgtgt
     3781 gattgccaga aacagcaacc cactcactag atataccgct ccaataatgg gttcatgtgc
     3841 aagctcactc aataacgaaa cgcttcacta ttgtgcacgt gccggggttg cttgggtatg
     3901 gcgtgccata cgggttctgc acatgtcacc cacgtgggtg tggcacgaga tatcgaggtg
     3961 gccatgtaaa aggcatttgc tagagggcag ctcgtccatg ccgctcgagc acaccgagtg
     4021 cgcaccataa atgcagcaca tgcctgtgca gcctatctcc tgtctctgtt acatcccacc
     4081 acctcctcac atatcccctt ccacccttaa accgcacagc agacatggcc aagtgcgcat
     4141 cgcgtgtcaa tgctggcgct gctgacgtcc gctgcctaaa ctgcatgaaa gctcctgcag
     4201 tcgcaagtag tccttttaac ttgttgtatc ggaacaaatg gttgtagtct gtgccgtgtg
     4261 acgatcttga gccgcctggt cggcaggtcc aatgtcggcc tggaccctgc acaccctgcc
     4321 caccctgccg ctgctcaccc gttcacgacc ccacaggtgc tcgcagcagt aattgtcgat
     4381 agatagcaac tgtgttctga ctattctttg accttccgca tttgggactg cggcggcaca
     4441 cctactatcc gcaatgcaca ccctggtgct ggtactgcct gcctttccgc gcgagcccac
     4501 accatgcagg cgcatagtaa tggactgtga ttgatttagg caactgtatg aaacctcttc
     4561 atcgtgcctt ggttgcttgc gataccctca cgtgagttca tatgaccaac gctggcacga
     4621 cccctgcagg atcaccagcg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     4681 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     4741 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     4801 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     4861 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     4921 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     4981 cgaggagcag gactaagcag tcaggcagag tcgttgttgg gtgtatggtt gcacaaagct
     5041 agacctcgag ctggtcggtt agaaagagat gcttgtgcga ctggatgtaa caggcacaat
     5101 ccgaggaaaa tgcaacagaa acgttatgat gtaacatctg agcacagtca ataaaattgc
     5161 atgttgtatg ccgcacacaa aagtgttgtt aaacatgcga tgtggttgtc aaatcattat
     5221 tcgcacaacc atggaccgaa cctctggcac gctgcatacg ctgtattcat tcacatcatg
     5281 ttttctactt gtcacatgct ctgcaccaag taactccgat tagttaggtt cgcacatgtc
     5341 atcggagtgg accacttcga atcatttggt tcgaagataa accggatccc ctatagtgag
     5401 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     5461 tgttacattg cacaagataa aataatatca tcatgaacaa taaaactgtc tgcttacata
     5521 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     5581 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     5641 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     5701 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     5761 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     5821 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     5881 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     5941 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     6001 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     6061 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     6121 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     6181 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     6241 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     6301 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     6361 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     6421 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     6481 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6541 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6601 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6661 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6721 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6781 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6841 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6901 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6961 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7021 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7081 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7141 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7201 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7261 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7321 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     7381 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     7441 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     7501 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     7561 ccgacaaaca acagataaaa cgaaaggccc agacttccga ctgagccttt cgttttattt
     7621 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     7681 cgttgtaaaa cgacggccag tcttaagctc gggcccacc
//