LOCUS pGG-UmpRbcS_46-bleGFP-tRbcS 4506 bp DNA circular UNA 12-APR-2023 DEFINITION . ACCESSION urn.local.jonasblomme.6n-cgtp4xx VERSION urn.local.jonasblomme.6n-cgtp4xx KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..46 /regulatory_class="promoter" /note="Ulva RbcS promoter" /modified_by="jonasblomme" /label="UmpRbcS" CDS join(81..88,586..1663) /gene="ble/GFP fusion" /note="Streptoalloteichus hindustanus ble gene fused with Aequoria victoria GFP gene (synthetic Ulva codon adapted construct)" /codon_start=1 /transl_table=11 /product="bleomycin resistance protein/GFP fusion protein" /protein_id="ABY60800.1" /db_xref="GI:164523505" /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW GREFALRDPAGNCVHFVAEEQVPKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA TYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIM ADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" intron 89..585 /gene="ble/GFP fusion" /note="derived from Ulva RbcS; enhancer" /number=1 /label="RbcsI" Linker 1668..1713 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker" terminator 1718..2106 /regulatory_class="terminator" /note="Ulva RbcS terminator" /modified_by="jonasblomme" /label="UmtRbcS" Linker 2111..2159 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker II" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II" /label="Linker II" primer complement(2196..2216) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 2277..2327 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 2328..3137 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 3284..3872 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 4471..4491 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 ccacctcctc acatatcccc ttccaccctt aaaccgcaca gcagacaaca gtattcagtc 61 gactggtacc aacaggctcc atggccaagt gcgcatcgcg tgtcaatgct ggcgctgctg 121 acgtccgctg cctaaactgc atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt 181 tgtatcggaa caaatggttg tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc 241 aggtccaatg tcggcctgga ccctgcacac cctgcccacc ctgccgctgc tcacccgttc 301 acgaccccac aggtgctcgc agcagtaatt gtcgatagat agcaactgtg ttctgactat 361 tctttgacct tccgcatttg ggactgcggc ggcacaccta ctatccgcaa tgcacaccct 421 ggtgctggta ctgcctgcct ttccgcgcga gcccacacca tgcaggcgca tagtaatgga 481 ctgtgattga tttaggcaac tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata 541 ccctcacgtg agttcatatg accaacgctg gcacgacccc tgcaggatca ccagcgccgt 601 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg 661 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct 721 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt 781 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga 841 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct 901 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggtac ccaagggcga 961 ggagctgttc accggtgtgg tccccatcct ggtggagctg gacggcgacg tgaacggcca 1021 caagttctcc gtctccggcg agggtgaggg tgacgccacc tacggcaagc tgaccctgaa 1081 gttcatctgc accaccggca agctgcccgt gccctggccc accctggtca ccaccctgac 1141 ctacggtgtg cagtgcttct cccgctaccc cgaccacatg aagcagcacg acttcttcaa 1201 gtccgccatg cccgagggct acgtgcagga gcgcaccatc ttcttcaagg acgacggcaa 1261 ctacaagacc cgcgccgagg tcaagttcga gggcgacacc ctggtgaacc gcatcgagct 1321 gaagggcatc gacttcaagg aggacggcaa catcctgggc cacaagctgg agtacaacta 1381 caactcccac aacgtgtaca tcatggccga caagcagaag aacggcatca aggtgaactt 1441 caagatccgc cacaacatcg aggacggctc cgtgcagctg gccgaccact accagcagaa 1501 cacccccatc ggcgatggcc ccgtgctgct gcccgacaac cactacctgt ccatccagtc 1561 cgccctgtcc aaggacccca acgagaagcg cgaccacatg gtcctgctgg agttcgtcac 1621 cgctgccggc atcacccacg gcatggacga gctgtacaag taatcaggtg gatcctagat 1681 aacctttacc ttcattccct gtaaattggt accctgcgca gtcaggcaga gtcgttgttg 1741 ggtgtatggt tgcacaaaac tagacctcga gctggtcggt tagaaagaga tgcttgtgcg 1801 actggatgta acaggcacaa tccgaggaaa atgcaacaga aacgttatga tgtaacatct 1861 gagcacagtc aataaaattg catgttgtat gccgcacaca aaagtgttgt taaacatgcg 1921 atgtggttgt caaatcatta ttcgcacaac catggaccga acctctggca cgctgcatac 1981 gctgtattca ttcacatcat gttttctact tgtcacatgc tctgcaccaa gtaactccga 2041 ttagttaggt tcgcacatgt catcggagtg gaccacttcg aatcatttgg ttcgaagata 2101 aaccggacta taagctaggt agtaactagt gcgactgtct agaatcgcgt atgcgagtgt 2161 atgagctcga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc 2221 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaata atatcatcat 2281 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc 2341 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata 2401 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc 2461 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag 2521 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt 2581 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat 2641 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt 2701 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat 2761 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg 2821 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc 2881 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg 2941 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc 3001 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc 3061 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc 3121 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc 3181 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt 3241 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 3301 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 3361 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 3421 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 3481 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 3541 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 3601 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 3661 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 3721 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 3781 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 3841 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 3901 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 3961 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 4021 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 4081 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 4141 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc 4201 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg 4261 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg 4321 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagac 4381 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc 4441 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc 4501 ccacct //