LOCUS pGG-pUM140_0016-B-ccdB-CmR-E-NOST_PIBT3 7852 bp DNA circular UNA 11-APR-2023 DEFINITION . ACCESSION urn.local.jonasblomme.g-cn2ko84 VERSION urn.local.jonasblomme.g-cn2ko84 KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/. FEATURES Location/Qualifiers promoter 1..1934 /modified_by="jonasblomme" /modified_by="mamee" /label="pUM140_0016" GG complement(1935..1945) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="B" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; B" /label="B" CDS complement(1952..2257) /vntifkey=4 /label="ccdB" UTR complement(2258..2576) /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.37%" /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" misc_feature complement(2599..3258) /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="14 CmR" /Transferred_Similarity="99.85%" /Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR (14 CmR)" /Alternative_Match="99.85%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" promoter complement(3312..3342) /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="lacUV5" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="14 CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (14 CmR)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (CmR)" /label="lacUV5" GG 3367..3377 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="E" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; E" /label="E" terminator 3378..3645 /vntifkey=43 /modified_by="mamee" /label="tNOS" Linker 3650..3698 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker II" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II" /label="Linker II" promoter 3711..4256 /label="Ulva prbcS" CDS join(4257..4264,4762..5128) /modified_by="mamee" /label="ble" intron 4265..4761 /gene="ble" /note="Ulva mutabilis RbcS intron 1; enhancer" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="RbcsI" /Transferred_Similarity="99.40%" /Primary_Match="99.40%; Adjusted-Interval-0:0/0; RbcsI" /modified_by="jonasblomme" /label="RbcsI" terminator 5129..5537 /label="UmtRbcS" primer complement(5542..5562) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 5623..5673 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 5674..6483 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 6630..7218 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 7817..7837 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag 61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc 121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg 181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc 241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag 301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg 361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg 421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta 481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa 541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg 601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg 661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg 721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc 781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg 841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga 901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt 961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc 1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat 1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga 1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga 1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt 1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt 1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg 1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct 1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta 1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg 1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca 1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc 1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac 1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt 1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc 1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct 1921 atcagtgatt aacaaacatg agaccgtcga cttatattcc ccagaacatc aggttaatgg 1981 cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg ataacggaga 2041 ctggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg atatgcacca 2101 ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc agggggatca 2161 ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac agacgataac 2221 ggctctctct tttataggtg taaaccttaa actgcatttc accagtccct gttctcgtca 2281 gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc ctgattttcc 2341 gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt gcttaccaga 2401 ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg tcaactgtca 2461 ctgtaatacg ctgcttcata gcacacctct ttttgacata cttcgggtat acatatcagt 2521 atatattctt ataccgcaaa aatcagcgcg caaatacgca tactgttatc tggcttttag 2581 taagccggat ccacgcgttt acgccccgcc ctgccactca tcgcagtact gttgtaattc 2641 attaagcatt ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag 2701 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa 2761 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc 2821 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta 2881 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact 2941 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact 3001 atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat 3061 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt 3121 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga 3181 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc 3241 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgccg gatcctaact 3301 caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg tgcctaatgc 3361 ggccgcggtc tcactgccta gagtcaagca gatcgttcaa acatttggca ataaagtttc 3421 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac 3481 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg 3541 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac 3601 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgaactat aagctaggta 3661 gtaactagtg cgactgtcta gaatcgcgta tgcgagtgta tgagctcgat cgttgttcgt 3721 acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag tccagatacc 3781 atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac tgcactaatc 3841 tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga gtactggcac 3901 aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg ctccaataat 3961 gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac gtgccggggt 4021 tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg tgtggcacga 4081 gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca tgccgctcga 4141 gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct cctgtctctg 4201 ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca gcagacatgg 4261 ccaagtgcgc atcgcgtgtc aatgctggcg ctgctgacgt ccgctgccta aactgcatga 4321 aagctcctgc agtcgcaagt agtcctttta acttgttgta tcggaacaaa tggttgtagt 4381 ctgtgccgtg tgacgatctt gagccgcctg gtcggcaggt ccaatgtcgg cctggaccct 4441 gcacaccctg cccaccctgc cgctgctcac ccgttcacga ccccacaggt gctcgcagca 4501 gtaattgtcg atagatagca actgtgttct gactattctt tgaccttccg catttgggac 4561 tgcggcggca cacctactat ccgcaatgca caccctggtg ctggtactgc ctgcctttcc 4621 gcgcgagccc acaccatgca ggcgcatagt aatggactgt gattgattta ggcaactgta 4681 tgaaacctct tcatcgtgcc ttggttgctt gcgataccct cacgtgagtt catatgacca 4741 acgctggcac gacccctgca ggatcaccag cgccgttccg gtgctcaccg cgcgcgacgt 4801 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga 4861 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca 4921 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc 4981 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga 5041 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt 5101 gcacttcgtg gccgaggagc aggactaagc agtcaggcag agtcgttgtt gggtgtatgg 5161 ttgcacaaag ctagacctcg agctggtcgg ttagaaagag atgcttgtgc gactggatgt 5221 aacaggcaca atccgaggaa aatgcaacag aaacgttatg atgtaacatc tgagcacagt 5281 caataaaatt gcatgttgta tgccgcacac aaaagtgttg ttaaacatgc gatgtggttg 5341 tcaaatcatt attcgcacaa ccatggaccg aacctctggc acgctgcata cgctgtattc 5401 attcacatca tgttttctac ttgtcacatg ctctgcacca agtaactccg attagttagg 5461 ttcgcacatg tcatcggagt ggaccacttc gaatcatttg gttcgaagat aaaccggatc 5521 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct 5581 caaaatctct gatgttacat tgcacaagat aaaataatat catcatgaac aataaaactg 5641 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg 5701 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat 5761 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag 5821 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga 5881 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct 5941 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa 6001 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg 6061 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag 6121 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat 6181 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat 6241 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta 6301 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc 6361 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat 6421 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc 6481 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac 6541 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca 6601 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 6661 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 6721 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 6781 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 6841 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 6901 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 6961 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 7021 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 7081 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 7141 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 7201 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 7261 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 7321 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 7381 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 7441 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 7501 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg 7561 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc 7621 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca 7681 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagacttcc gactgagcct 7741 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt 7801 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggcccac ct //