LOCUS pGG-pUM140_0016-BleR-E2A-B-ccdB-CmR-G-UmtRbcS 7121 bp DNA circular UNA 11-APR-2023 DEFINITION . ACCESSION urn.local.jonasblomme.h-cn2ko84 VERSION urn.local.jonasblomme.h-cn2ko84 KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/. FEATURES Location/Qualifiers promoter 1..1934 /modified_by="jonasblomme" /label="pUM140_0016" CDS join(1939..1946,2444..2807) /gene="ble" /note="derived from Streptoalloteichus hindustanus, adapted to Chlamydomonas reinhardtii and Ulva mutabilis codon usage" /codon_start=1 /transl_table=11 /product="bleomycin resistance protein" /protein_id="ABY50194.1" /db_xref="GI:163962062" /translation="MAKITSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW GREFALRDPAGNCVHFVAEEQD" intron 1947..2443 /label="RbcsI" CDS 2808..2876 /label="E2A" GG complement(2882..2892) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="B" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; B" /label="B" CDS complement(2899..3204) /vntifkey=4 /label="ccdB" UTR complement(3205..3523) /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.37%" /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" misc_feature complement(3546..4205) /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="14 CmR" /Transferred_Similarity="99.85%" /Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR (14 CmR)" /Alternative_Match="99.85%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" promoter complement(4259..4289) /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="lacUV5" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="14 CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (14 CmR)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (CmR)" /label="lacUV5" GG 4314..4324 /%_Identity=100 /Motif="GGTCTCAGTAT" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="G" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; G overlap (G)" /modified_by="mamee" /label="G" terminator 4333..4721 /regulatory_class="terminator" /note="Ulva RbcS terminator" /modified_by="jonasblomme" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="UmtRbcS" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; UmtRbcS" /label="UmtRbcS" Linker 4726..4774 /created_by="wacae" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Linker II" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II" /label="Linker II" primer complement(4811..4831) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 4892..4942 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 4943..5752 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 5899..6487 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 7086..7106 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag 61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc 121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg 181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc 241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag 301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg 361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg 421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta 481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa 541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg 601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg 661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg 721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc 781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg 841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga 901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt 961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc 1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat 1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga 1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga 1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt 1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt 1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg 1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct 1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta 1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg 1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca 1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc 1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac 1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt 1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc 1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct 1921 atcagtgatt aacaaacaat ggccaagtgc gcatcgcgtg tcaatgctgg cgctgctgac 1981 gtccgctgcc taaactgcat gaaagctcct gcagtcgcaa gtagtccttt taacttgttg 2041 tatcggaaca aatggttgta gtctgtgccg tgtgacgatc ttgagccgcc tggtcggcag 2101 gtccaatgtc ggcctggacc ctgcacaccc tgcccaccct gccgctgctc acccgttcac 2161 gaccccacag gtgctcgcag cagtaattgt cgatagatag caactgtgtt ctgactattc 2221 tttgaccttc cgcatttggg actgcggcgg cacacctact atccgcaatg cacaccctgg 2281 tgctggtact gcctgccttt ccgcgcgagc ccacaccatg caggcgcata gtaatggact 2341 gtgattgatt taggcaactg tatgaaacct cttcatcgtg ccttggttgc ttgcgatacc 2401 ctcacgtgag ttcatatgac caacgctggc acgacccctg caggatcacc agcgccgttc 2461 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt 2521 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt 2581 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc 2641 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg 2701 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc 2761 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggacggc agcggccagt 2821 gcacgaacta cgccctgctg aagctggcgg gcgacgtgga gagcaacccg ggcccgggct 2881 caacatgaga ccgtcgactt atattcccca gaacatcagg ttaatggcgt ttttgatgtc 2941 attttcgcgg tggctgagat cagccacttc ttccccgata acggagactg gcacactggc 3001 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc 3061 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc 3121 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt 3181 ataggtgtaa accttaaact gcatttcacc agtccctgtt ctcgtcagca aaagagccgt 3241 tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt 3301 cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga 3361 catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg 3421 cttcatagca cacctctttt tgacatactt cgggtataca tatcagtata tattcttata 3481 ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcca 3541 cgcgtttacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg 3601 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc 3661 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata 3721 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac 3781 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct 3841 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa 3901 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc 3961 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga 4021 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc 4081 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca 4141 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc 4201 tccattttag cttccttagc tcctgaaaat ctcgccggat cctaactcaa aatccacaca 4261 ttatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgcggc cgcggtctca 4321 gtatctgact gcgcagtcag gcagagtcgt tgttgggtgt atggttgcac aaaactagac 4381 ctcgagctgg tcggttagaa agagatgctt gtgcgactgg atgtaacagg cacaatccga 4441 ggaaaatgca acagaaacgt tatgatgtaa catctgagca cagtcaataa aattgcatgt 4501 tgtatgccgc acacaaaagt gttgttaaac atgcgatgtg gttgtcaaat cattattcgc 4561 acaaccatgg accgaacctc tggcacgctg catacgctgt attcattcac atcatgtttt 4621 ctacttgtca catgctctgc accaagtaac tccgattagt taggttcgca catgtcatcg 4681 gagtggacca cttcgaatca tttggttcga agataaaccg gactataagc taggtagtaa 4741 ctagtgcgac tgtctagaat cgcgtatgcg agtgtatgag ctcgatatcc cctatagtga 4801 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg 4861 atgttacatt gcacaagata aaataatatc atcatgaaca ataaaactgt ctgcttacat 4921 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt 4981 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca 5041 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa 5101 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct 5161 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg 5221 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga 5281 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc 5341 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg 5401 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt 5461 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac 5521 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat 5581 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg 5641 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa 5701 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt 5761 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc 5821 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga 5881 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 5941 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 6001 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 6061 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 6121 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 6181 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 6241 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 6301 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 6361 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 6421 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 6481 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 6541 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 6601 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 6661 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 6721 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac 6781 gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc 6841 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc 6901 cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc 6961 accgacaaac aacagataaa acgaaaggcc cagacttccg actgagcctt tcgttttatt 7021 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg 7081 acgttgtaaa acgacggcca gtcttaagct cgggcccacc t //