LOCUS pGGIB-U2-A-ccdB-GmR-G-U3 3782 bp DNA circular 21-MAR-2023 DEFINITION . ACCESSION urn.local...9p-bn3sg4o VERSION urn.local...9p-bn3sg4o KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_difference 1..18 /created_by="laurenspauwels" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="I-SceI site" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; I-SceI site" /label="I-SceI site" misc_feature 19..58 /created_by="lapau" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="U2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; U2" /label="U2" /note="Geneious type: Golden Gibson Cloning site" GG complement(67..77) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="A" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; A" /label="A" CDS complement(84..389) /standard_name="ccdB" UTR complement(390..708) /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.37%" /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" misc_feature complement(731..1264) /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="GmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GmR" /label="GmR" /note="Geneious type: Bacterial Selection" promoter complement(1318..1348) /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="lacUV5" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="14 CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (14 CmR)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (CmR)" /label="lacUV5" GG 1373..1383 /%_Identity=100 /Motif="GGTCTCAGTAT" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="G" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; G" /label="G" misc_feature 1390..1429 /created_by="lapau" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="U3" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; U3" /label="U3" /note="Geneious type: Golden Gibson Cloning site" misc_difference complement(1430..1447) /created_by="laurenspauwels" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="I-SceI site" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; I-SceI site" /label="I-SceI site" primer complement(1475..1495) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 1556..1606 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 1607..2416 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 2563..3151 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 3751..3771 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 attaccctgt tatccctagc tgggagttcg tagacggaaa caaacgcaga atccaagcct 61 cgagatacct tgagaccgtc gacttatatt ccccagaaca tcaggttaat ggcgtttttg 121 atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga gactggcaca 181 ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa 241 agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt 301 cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata acggctctct 361 cttttatagg tgtaaacctt aaactgcatt tcaccagtcc ctgttctcgt cagcaaaaga 421 gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca 481 gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca gaccggagat 541 attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata 601 cgctgcttca tagcacacct ctttttgaca tacttcgggt atacatatca gtatatattc 661 ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt agtaagccgg 721 atccacgcgt ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta 781 tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga 841 tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa 901 tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg 961 gctgctcaaa cctgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga 1021 aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg 1081 gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag 1141 cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg 1201 agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta 1261 acattttagc ttccttagct cctgaaaatc tcgccggatc ctaactcaaa atccacacat 1321 tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgcggcc gcggtctcag 1381 tattctagag cactgaaggt cctcaatcgc actggaaaca tcaaggtcgt agggataaca 1441 gggtaatgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct 1501 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg 1561 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca 1621 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa 1681 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc 1741 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga 1801 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt 1861 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt 1921 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt 1981 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt 2041 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga 2101 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc 2161 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga 2221 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca 2281 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct 2341 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct 2401 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct 2461 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt 2521 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 2581 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 2641 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 2701 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 2761 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 2821 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 2881 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 2941 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 3001 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 3061 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 3121 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 3181 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 3241 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 3301 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 3361 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 3421 cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca 3481 aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg 3541 cgtcctgccc gccacccctt cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg 3601 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc 3661 atccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc 3721 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc 3781 cc //