LOCUS       pCDB-Cas9-PDS           8959 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...r-cixsr3o
VERSION     urn.local...r-cixsr3o
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     attL1           1..100
                     /Color="#00cccc"
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL1"
                     /Transferred_Similarity="99.00%"
                     /Primary_Match="99.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:li-eztyqs1"">attL1</a>"
                     /label="attL1"
     promoter        107..970
                     /regulatory_class="promoter"
                     /gene="CaMV 35S"
                     /note="internal BsaI recognition site removed"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S - Golden Gate"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:6v-dgycryx"">pCaMV 35S - Golden
                     Gate</a>)"
                     /label="p35S"
     Linker          975..998
                     /gene="B-dummy"
                     /note="default module for GreenGate"
                     /modified_by="mamee"
                     /label="B-dummy 5'UTR"
     CDS             1005..5108
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AtCas9"
                     /Transferred_Similarity="100.00%"
                     /Adjusted_Transferred_Similarity="99.13%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/-36
                     (adjusted to 99.13%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:.:95-dgycryy"">AtCas9</a>"
                     /label="AtCAS9"
     NLS             5121..5141
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     Linker          5149..5194
                     /gene="D-dummy"
                     /note="default module for GreenGate"
                     /modified_by="mamee"
                     /label="D-dummy 3'UTR"
     terminator      5199..5845
                     /regulatory_class="terminator"
                     /note="RBCS terminator (from Pisum sativum)"
                     /modified_by="mamee"
                     /label="tRbcS"
     promoter        5852..6239
                     /vntifkey=29
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AtU6-26"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:79-dgycryx"">AtU6-26</a>"
                     /label="AtU6-26"
     N20             6240..6259
                     /created_by="cdebruyn"
                     /label="N20"
     Scaffold        6260..6342
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpCas9 Scaffold"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Scaffold
                     (<a href=""urn:local:.:7o-dgycryx"">SpCas9 Scaffold</a>)"
                     /label="Scaffold"
     attL2           complement(6348..6447)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ll-eztyzh8"">attL2</a>"
                     /label="attL2"
     primer          complement(6485..6505)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        6566..6616
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    6617..7426
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      7573..8161
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    8710..8877
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer          8928..8948
                     /label="GWM13F"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct taacctccga tccattcggt
      121 ccccagatta gccttttcaa tttcagaaag aatgctaacc cacagatggt tagagaggct
      181 tacgcagcac gtctcatcaa gacgatctac ccgagcaata atctccagga aatcaaatac
      241 cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat caagaacaca
      301 gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg
      361 cttcacaaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcccactgaa
      421 tcaaaggcca tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact
      481 ggcgaacagt tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac
      541 atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac
      601 caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat
      661 tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa
      721 tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc
      781 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
      841 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
      901 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gagaacacgg
      961 gggacgagct aacagtattc agtcgactgg taccaacagg ctccatggat aagaagtact
     1021 ctatcggact cgatatcgga actaactctg tgggatgggc tgtgatcacc gatgagtaca
     1081 aggtgccatc taagaagttc aaggttctcg gaaacaccga taggcactct atcaagaaaa
     1141 accttatcgg tgctctcctc ttcgattctg gtgaaactgc tgaggctacc agactcaaga
     1201 gaaccgctag aagaaggtac accagaagaa agaacaggat ctgctacctc caagagatct
     1261 tctctaacga gatggctaaa gtggatgatt cattcttcca caggctcgaa gagtcattcc
     1321 tcgtggaaga agataagaag cacgagaggc accctatctt cggaaacatc gttgatgagg
     1381 tggcatacca cgagaagtac cctactatct accacctcag aaagaagctc gttgattcta
     1441 ctgataaggc tgatctcagg ctcatctacc tcgctctcgc tcacatgatc aagttcagag
     1501 gacacttcct catcgagggt gatctcaacc ctgataactc tgatgtggat aagttgttca
     1561 tccagctcgt gcagacctac aaccagcttt tcgaagagaa ccctatcaac gcttcaggtg
     1621 tggatgctaa ggctatcctc tctgctaggc tctctaagtc aagaaggctt gagaacctca
     1681 ttgctcagct ccctggtgag aagaagaacg gacttttcgg aaacttgatc gctctctctc
     1741 tcggactcac ccctaacttc aagtctaact tcgatctcgc tgaggatgca aagctccagc
     1801 tctcaaagga tacctacgat gatgatctcg ataacctcct cgctcagatc ggagatcagt
     1861 acgctgattt gttcctcgct gctaagaacc tctctgatgc tatcctcctc agtgatatcc
     1921 tcagagtgaa caccgagatc accaaggctc cactctcagc ttctatgatc aagagatacg
     1981 atgagcacca ccaggatctc acacttctca aggctcttgt tagacagcag ctcccagaga
     2041 agtacaaaga gattttcttc gatcagtcta agaacggata cgctggttac atcgatggtg
     2101 gtgcatctca agaagagttc tacaagttca tcaagcctat cctcgagaag atggatggaa
     2161 ccgaggaact cctcgtgaag ctcaatagag aggatcttct cagaaagcag aggaccttcg
     2221 ataacggatc tatccctcat cagatccacc tcggagagtt gcacgctatc cttagaaggc
     2281 aagaggattt ctacccattc ctcaaggata acagggaaaa gattgagaag attctcacct
     2341 tcagaatccc ttactacgtg ggacctctcg ctagaggaaa ctcaagattc gcttggatga
     2401 ccagaaagtc tgaggaaacc atcacccctt ggaacttcga agaggtggtg gataagggtg
     2461 ctagtgctca gtctttcatc gagaggatga ccaacttcga taagaacctt ccaaacgaga
     2521 aggtgctccc taagcactct ttgctctacg agtacttcac cgtgtacaac gagttgacca
     2581 aggttaagta cgtgaccgag ggaatgagga agcctgcttt tttgtcaggt gagcaaaaga
     2641 aggctatcgt tgatctcttg ttcaagacca acagaaaggt gaccgtgaag cagctcaaag
     2701 aggattactt caagaaaatc gagtgcttcg attcagttga gatttctggt gttgaggata
     2761 ggttcaacgc atctctcgga acctaccacg atctcctcaa gatcattaag gataaggatt
     2821 tcttggataa cgaggaaaac gaggatatct tggaggatat cgttcttacc ctcaccctct
     2881 ttgaagatag agagatgatt gaagaaaggc tcaagaccta cgctcatctc ttcgatgata
     2941 aggtgatgaa gcagttgaag agaagaagat acactggttg gggaaggctc tcaagaaagc
     3001 tcattaacgg aatcagggat aagcagtctg gaaagacaat ccttgatttc ctcaagtctg
     3061 atggattcgc taacagaaac ttcatgcagc tcatccacga tgattctctc acctttaaag
     3121 aggatatcca gaaggctcag gtttcaggac agggtgatag tctccatgag catatcgcta
     3181 acctcgctgg atctcctgca atcaagaagg gaatcctcca gactgtgaag gttgtggatg
     3241 agttggtgaa ggtgatggga aggcataagc ctgagaacat cgtgatcgaa atggctagag
     3301 agaaccagac cactcagaag ggacagaaga actctaggga aaggatgaag aggatcgagg
     3361 aaggtatcaa agagcttgga tctcagatcc tcaaagagca ccctgttgag aacactcagc
     3421 tccagaatga gaagctctac ctctactacc tccagaacgg aagggatatg tatgtggatc
     3481 aagagttgga tatcaacagg ctctctgatt acgatgttga tcatatcgtg ccacagtcat
     3541 tcttgaagga tgattctatc gataacaagg tgctcaccag gtctgataag aacaggggta
     3601 agagtgataa cgtgccaagt gaagaggttg tgaagaaaat gaagaactat tggaggcagc
     3661 tcctcaacgc taagctcatc actcagagaa agttcgataa cttgactaag gctgagaggg
     3721 gaggactctc tgaattggat aaggcaggat tcatcaagag gcagcttgtg gaaaccaggc
     3781 agatcactaa gcacgttgca cagatcctcg attctaggat gaacaccaag tacgatgaga
     3841 acgataagtt gatcagggaa gtgaaggtta tcaccctcaa gtcaaagctc gtgtctgatt
     3901 tcagaaagga tttccaattc tacaaggtga gggaaatcaa caactaccac cacgctcacg
     3961 atgcttacct taacgctgtt gttggaaccg ctctcatcaa gaagtatcct aagctcgagt
     4021 cagagttcgt gtacggtgat tacaaggtgt acgatgtgag gaagatgatc gctaagtctg
     4081 agcaagagat cggaaaggct accgctaagt atttcttcta ctctaacatc atgaatttct
     4141 tcaagaccga gattaccctc gctaacggtg agatcagaaa gaggccactc atcgagacaa
     4201 acggtgaaac aggtgagatc gtgtgggata agggaaggga tttcgctacc gttagaaagg
     4261 tgctctctat gccacaggtg aacatcgtta agaaaaccga ggtgcagacc ggtggattct
     4321 ctaaagagtc tatcctccct aagaggaact ctgataagct cattgctagg aagaaggatt
     4381 gggaccctaa gaaatacggt ggtttcgatt ctcctaccgt ggcttactct gttctcgttg
     4441 tggctaaggt tgagaaggga aagagtaaga agctcaagtc tgttaaggaa cttctcggaa
     4501 tcactatcat ggaaaggtca tctttcgaga agaacccaat cgatttcctc gaggctaagg
     4561 gatacaaaga ggttaagaag gatctcatca tcaagctccc aaagtactca ctcttcgaac
     4621 tcgagaacgg tagaaagagg atgctcgctt ctgctggtga gcttcaaaag ggaaacgagc
     4681 ttgctctccc atctaagtac gttaactttc tttacctcgc ttctcactac gagaagttga
     4741 agggatctcc agaagataac gagcagaagc aacttttcgt tgagcagcac aagcactact
     4801 tggatgagat catcgagcag atctctgagt tctctaaaag ggtgatcctc gctgatgcaa
     4861 acctcgataa ggtgttgtct gcttacaaca agcacagaga taagcctatc agggaacagg
     4921 cagagaacat catccatctc ttcaccctta ccaacctcgg tgctcctgct gctttcaagt
     4981 acttcgatac aaccatcgat aggaagagat acacctctac caaagaagtg ctcgatgcta
     5041 ccctcatcca tcagtctatc actggactct acgagactag gatcgatctc tcacagctcg
     5101 gtggtgattc aagggctgat cctaagaaga agaggaaggt ttgatcaggt ggatcctaga
     5161 taacctttac cttcattccc tgtaaattgg taccctgcaa agctttcgtt cgtatcatcg
     5221 gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat tgcgcacaca ccagaatcct
     5281 actgagtttg agtattatgg cattgggaaa actgtttttc ttgtaccatt tgttgtgctt
     5341 gtaatttact gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg
     5401 agaagagtta atgaatgata tggtcctttt gttcattctc aaattaatat tatttgtttt
     5461 ttctcttatt tgttgtgtgt tgaatttgaa attataagag atatgcaaac attttgtttt
     5521 gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag gagtaaaaca
     5581 cttgtagttg taccattatg cttattcact aggcaacaaa tatattttca gacctagaaa
     5641 agctgcaaat gttactgaat acaagtatgt cctcttgtgt tttagacatt tatgaacttt
     5701 cctttatgta attttccaga atccttgtca gattctaatc attgctttat aattatagtt
     5761 atactcatgg atttgtagtt gagtatgaaa atatttttta atgcatttta tgacttgcca
     5821 attgattgac aacatgcatc aatcgactac tctttttttc ttcttcttcg ttcatacagt
     5881 ttttttttgt ttatcagctt acattttctt gaaccgtagc tttcgttttc ttctttttaa
     5941 ctttccattc ggagtttttg tatcttgttt catagtttgt cccaggatta gaatgattag
     6001 gcatcgaacc ttcaagaatt tgattgaata aaacatcttc attcttaaga tatgaagata
     6061 atcttcaaaa ggcccctggg aatctgaaag aagagaagca ggcccattta tatgggaaag
     6121 aacaatagta tttcttatat aggcccattt aagttgaaaa caatcttcaa aagtcccaca
     6181 tcgcttagat aagaaaacga agctgagttt atatacagct agagtcgaag tagtgattgt
     6241 ttggatgctg tgatgccatg ttttagagct agaaatagca agttaaaata aggctagtcc
     6301 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttgtattacc cagctttctt
     6361 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     6421 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     6481 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     6541 cattgcacaa gataaaataa tatcatcatg aacaataaaa ctgtctgctt acataaacag
     6601 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     6661 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     6721 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     6781 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     6841 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     6901 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     6961 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     7021 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     7081 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     7141 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     7201 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     7261 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     7321 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     7381 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     7441 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     7501 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     7561 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7621 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7681 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7741 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7801 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7861 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7921 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7981 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8041 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8101 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8161 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8221 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8281 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8341 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8401 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
     8461 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
     8521 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
     8581 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
     8641 aaacaacaga taaaacgaaa ggcccagact tccgactgag cctttcgttt tatttgatgc
     8701 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     8761 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     8821 aaacgaaagg cccagacttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     8881 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
     8941 cagtcttaag ctcgggccc
//