LOCUS pEN-R2-LinkerTurboID-L3 4181 bp ds-DNA circular 10-FEB-2023 DEFINITION . ACCESSION VERSION . KEYWORDS pUC57. SOURCE synthetic DNA construct (unknown) ORGANISM synthetic DNA construct. REFERENCE 1 (bases 1 to 4181) AUTHORS . TITLE Direct Submission JOURNAL Exported 2020��9��11�� from SnapGene 2.3.2 http://www.snapgene.com COMMENT This file is created by Vector NTI http://www.invitrogen.com/. FEATURES Location/Qualifiers attR2 complement(1..126) /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2" Linker 128..322 /modified_by="mamee" /label="linker" CDS 326..1288 /label="TurboID" tag 1292..1372 /modified_by="mamee" /label="3xHA" attL3 complement(1377..1472) /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attL3" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL3" primer complement(1530..1546) /invitrogen=2010000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 reverse primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 reverse primer (pDONRP4-P3 - M13 reverse primer)" /label="M13 reverse primer" rep_origin complement(1932..2520) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" misc_feature complement(2691..3551) /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="99.77%" /Primary_Match="99.77%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" promoter complement(3552..3656) /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" primer 4110..4126 /invitrogen=2000000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-40) forward primer (pDONRP4-P3 - M13 (-40) forward primer)" /label="M13 (-40) forward primer" primer 4130..4145 /invitrogen=1980000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-20) forward primer (pDONRP4-P3 - M13 (-20) forward primer)" /label="M13 (-20) forward primer" ORIGIN 1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat 61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa 121 agtgggaggt ggcggaggtt ctggcggtgg aggatccgga ggtggcgggt ccggtggtgg 181 cggatcaggt ggaggaggtt cgggaggcgg aggtagtgga ggcggtggga gcggcggagg 241 tgggagcggt ggcggaggga gtggaggtgg aggctcgggc ggaggtggct ccggcggagg 301 tgggtctgga ggcggtggct cggacaaaga caataccgtg cccctcaaac taattgcatt 361 gcttgccaat ggtgagttcc attctgggga acaacttggg gagactctgg gcatgtcccg 421 agcggccatt aataagcaca tacagacttt gagggactgg ggtgtcgatg tgtttacagt 481 accgggtaag ggatattctc tgccggagcc gataccttta ctcaatgcaa agcagatttt 541 aggacagctg gatggcggga gcgtggctgt cctcccggtg gtcgatagta caaatcaata 601 tcttttggac aggatcggtg agctgaaatc tggagatgct tgcatagcgg aataccaaca 661 agctggtagg ggatctcgag gccgaaagtg gttttcaccc tttggggcca acttatactt 721 atccatgttc tggcgtctga aacgtggccc ggcagccata gggctagggc ccgtaattgg 781 gatcgttatg gcggaagcgt tacgaaagtt gggagccgat aaagtcagag taaagtggcc 841 aaatgatctt tatttacaag atagaaagct ggcgggcatt cttgttgagc ttgccggaat 901 aacaggcgat gccgcacaga tcgttatagg ggccgggatt aatgtggcta tgaggagggt 961 cgaagaaagt gttgtgaatc aaggatggat tactctccaa gaggcgggta taaatcttga 1021 tcgaaatact ctagctgcaa ccttgatacg agagctaaga gcagcactag aattgtttga 1081 acaagaaggg ctggccccat atctccctcg atgggagaag ttggacaatt ttataaacag 1141 accggtgaag ttaatcattg gagacaaaga aattttcggt ataagccgtg ggatcgataa 1201 acagggtgct cttctccttg agcaggacgg tgtcatcaag ccttggatgg gaggcgaaat 1261 ctctctccga agcgccgaga agaagcttgc ctacccatac gatgttccag attacgctta 1321 cccatacgat gttccagatt acgcttaccc atacgatgtt ccagattacg cttgaccaac 1381 tttattatac aaagttggca ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca 1441 ggtcactatc agtcaaaata aaatcattat ttatcggatc ccgggcccgt cgactgcaga 1501 ggcctgcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 1561 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 1621 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 1681 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 1741 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 1801 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 1861 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 1921 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 1981 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 2041 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 2101 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 2161 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 2221 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 2281 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 2341 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 2401 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 2461 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 2521 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 2581 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 2641 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 2701 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 2761 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 2821 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 2881 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 2941 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 3001 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 3061 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 3121 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 3181 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 3241 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 3301 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 3361 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 3421 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 3481 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 3541 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 3601 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 3661 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 3721 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac 3781 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 3841 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat 3901 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 3961 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg 4021 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct 4081 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg 4141 gccagtgaat tcgagctcgg tacctcgcga atgcatctag a //