LOCUS pGG-D-TurboID-HIS-E 3489 bp DNA circular UNA 10-FEB-2023 DEFINITION . ACCESSION urn.local...18-de9m5yj KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers GG 1..11 /created_by="mamee" /modified_by="mamee" /label="D*" CDS 14..994 /label="TurboID" tag 995..1012 /modified_by="mamee" /label="6xHIS" GG complement(1013..1023) /created_by="mamee" /modified_by="mamee" /label="E*" primer complement(1055..1071) /invitrogen=2010000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 reverse primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 reverse primer (pDONRP4-P3 - M13 reverse primer)" /label="M13 reverse primer" rep_origin complement(1457..2045) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" misc_feature 2377..2910 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="GmR" /Transferred_Similarity="99.81%" /Primary_Match="99.81%; Adjusted-Interval-0:0/0; GmR" /label="GmR" /note="Geneious type: Bacterial Selection" primer 3383..3399 /invitrogen=2000000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-40) forward primer (pDONRP4-P3 - M13 (-40) forward primer)" /label="M13 (-40) forward primer" primer 3403..3418 /invitrogen=1980000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-20) forward primer (pDONRP4-P3 - M13 (-20) forward primer)" /label="M13 (-20) forward primer" ORIGIN 1 ggtctcaaag ggaatgaaag acaatactgt gcctctgaag ctgatcgctc tcctggctaa 61 tggcgagttc catagtggcg aacagctggg agaaaccctg ggcatgtcca gggccgctat 121 caacaagcac attcagactc tgcgcgactg gggcgtggac gtgttcaccg tgcccggaaa 181 gggctactct ctgcccgagc ctatcccgct gctgaacgct aaacagattc tgggacagct 241 ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc accaatcagt acctgctgga 301 tcgaatcggc gagctgaaga gtggggatgc ttgcattgca gaatatcagc aggcagggag 361 aggaagcaga gggaggaaat ggttctctcc ttttggagct aacctgtacc tgagtatgtt 421 ttggcgcctg aagcggggac cagcagcaat cggcctgggc ccggtcatcg gaattgtcat 481 ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc ccaatgacct 541 gtatctgcag gatagaaagc tggcaggcat cctggtggag ctggccggaa taacaggcga 601 tgctgcacag atcgtcattg gcgccgggat taacgtggct atgaggcgcg tggaggaaag 661 cgtggtcaat cagggctgga tcacactgca ggaagcaggg attaacctgg acaggaatac 721 tctggccgct acgctgatcc gagagctgcg ggcagccctg gaactgttcg agcaggaagg 781 cctggctcca tatctgccac ggtgggagaa gctggataac ttcatcaata gacccgtgaa 841 gctgatcatt ggggacaaag agattttcgg gattagccgg gggattgata aacagggagc 901 cctgctgctg gaacaggacg gagttatcaa accctggatg ggcggagaaa tcagtctgcg 961 gtctgccgaa aagaagcttg cggccgcact cgagcaccac caccaccacc acaatctgag 1021 acctctgcct gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 1081 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 1141 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 1201 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 1261 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 1321 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 1381 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 1441 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 1501 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 1561 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 1621 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 1681 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 1741 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 1801 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 1861 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 1921 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 1981 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 2041 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 2101 gttaagggat tttggtcatg aggcgcacac cgtggaaacg gatgaaggca cgaacccagt 2161 tgacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact 2221 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt 2281 gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg 2341 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag caacgatgtt 2401 acgcagcagg gcagtcgccc taaaacaaag ttaggtggct caagtatggg catcattcgc 2461 acatgtaggc tcggccctga ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt 2521 cgtgagttcg gcgacgtagc cacctactcc caacatcagc cggactccga ttacctcggg 2581 aacttgctcc gtagtaagac attcatcgcg cttgctgcct tcgaccaaga agcggttgtt 2641 ggcgctctcg cggcttacgt tctgcccagg tttgagcagc cgcgtagtga gatctatatc 2701 tatgatctcg cagtctccgg cgagcaccgg aggcagggca ttgccaccgc gctcatcaat 2761 ctcctcaagc atgaggccaa cgcgcttggt gcttatgtga tctacgtgca agcagattac 2821 ggtgacgatc ccgcagtggc tctctataca aagttgggca tacgggaaga agtgatgcac 2881 tttgatatcg acccaagtac cgccacctaa caattcgttc aagccgagat cggcttcccg 2941 gccgcggagt tgttcggtaa attgtcacaa cgccgctcat gacattaacc tataaaaata 3001 ggcgtatcac gaggcccttt tgtctcgcgc gtttcggtga tgacggtgaa aacctctgac 3061 acatgcagct cccggagaag gtcacagctt gtctgtaagc ggatgccggg agcagacaag 3121 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat 3181 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 3241 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 3301 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 3361 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 3421 aattcgagct cggtacctcg cgaatgcatc tagatatcgg atcccgggcc cgtcgactgc 3481 agaggtacg //