LOCUS pGG-C-TurboID-HIS-D 3487 bp DNA circular UNA 10-FEB-2023 DEFINITION . ACCESSION urn.local...10-de9m5ye KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers GG 1..11 /created_by="mamee" /modified_by="mamee" /label="C*" CDS 12..992 /label="TurboID" tag 993..1010 /modified_by="mamee" /label="6xHIS" GG complement(1011..1021) /created_by="mamee" /modified_by="mamee" /label="D*" primer complement(1053..1069) /invitrogen=2010000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 reverse primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 reverse primer (pDONRP4-P3 - M13 reverse primer)" /label="M13 reverse primer" rep_origin complement(1455..2043) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" misc_feature 2375..2908 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="GmR" /Transferred_Similarity="99.81%" /Primary_Match="99.81%; Adjusted-Interval-0:0/0; GmR" /label="GmR" /note="Geneious type: Bacterial Selection" primer 3381..3397 /invitrogen=2000000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-40) forward primer (pDONRP4-P3 - M13 (-40) forward primer)" /label="M13 (-40) forward primer" primer 3401..3416 /invitrogen=1980000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-20) forward primer (pDONRP4-P3 - M13 (-20) forward primer)" /label="M13 (-20) forward primer" ORIGIN 1 ggtctcacac catgaaagac aatactgtgc ctctgaagct gatcgctctc ctggctaatg 61 gcgagttcca tagtggcgaa cagctgggag aaaccctggg catgtccagg gccgctatca 121 acaagcacat tcagactctg cgcgactggg gcgtggacgt gttcaccgtg cccggaaagg 181 gctactctct gcccgagcct atcccgctgc tgaacgctaa acagattctg ggacagctgg 241 acggcgggag cgtggcagtc ctgcctgtgg tcgactccac caatcagtac ctgctggatc 301 gaatcggcga gctgaagagt ggggatgctt gcattgcaga atatcagcag gcagggagag 361 gaagcagagg gaggaaatgg ttctctcctt ttggagctaa cctgtacctg agtatgtttt 421 ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg 481 cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt caaatggccc aatgacctgt 541 atctgcagga tagaaagctg gcaggcatcc tggtggagct ggccggaata acaggcgatg 601 ctgcacagat cgtcattggc gccgggatta acgtggctat gaggcgcgtg gaggaaagcg 661 tggtcaatca gggctggatc acactgcagg aagcagggat taacctggac aggaatactc 721 tggccgctac gctgatccga gagctgcggg cagccctgga actgttcgag caggaaggcc 781 tggctccata tctgccacgg tgggagaagc tggataactt catcaataga cccgtgaagc 841 tgatcattgg ggacaaagag attttcggga ttagccgggg gattgataaa cagggagccc 901 tgctgctgga acaggacgga gttatcaaac cctggatggg cggagaaatc agtctgcggt 961 ctgccgaaaa gaagcttgcg gccgcactcg agcaccacca ccaccaccac aaggtgagac 1021 ctctgcctgc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 1081 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 1141 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 1201 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 1261 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 1321 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 1381 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 1441 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 1501 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 1561 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 1621 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 1681 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 1741 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 1801 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 1861 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 1921 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 1981 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 2041 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 2101 taagggattt tggtcatgag gcgcacaccg tggaaacgga tgaaggcacg aacccagttg 2161 acataagcct gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg 2221 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt 2281 tatgactgtt tttttgtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc 2341 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcagca acgatgttac 2401 gcagcagggc agtcgcccta aaacaaagtt aggtggctca agtatgggca tcattcgcac 2461 atgtaggctc ggccctgacc aagtcaaatc catgcgggct gctcttgatc ttttcggtcg 2521 tgagttcggc gacgtagcca cctactccca acatcagccg gactccgatt acctcgggaa 2581 cttgctccgt agtaagacat tcatcgcgct tgctgccttc gaccaagaag cggttgttgg 2641 cgctctcgcg gcttacgttc tgcccaggtt tgagcagccg cgtagtgaga tctatatcta 2701 tgatctcgca gtctccggcg agcaccggag gcagggcatt gccaccgcgc tcatcaatct 2761 cctcaagcat gaggccaacg cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg 2821 tgacgatccc gcagtggctc tctatacaaa gttgggcata cgggaagaag tgatgcactt 2881 tgatatcgac ccaagtaccg ccacctaaca attcgttcaa gccgagatcg gcttcccggc 2941 cgcggagttg ttcggtaaat tgtcacaacg ccgctcatga cattaaccta taaaaatagg 3001 cgtatcacga ggcccttttg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 3061 atgcagctcc cggagaaggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 3121 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca 3181 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 3241 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 3301 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 3361 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa 3421 ttcgagctcg gtacctcgcg aatgcatcta gatatcggat cccgggcccg tcgactgcag 3481 aggtacg //