LOCUS pGG-D-linker-TurboID-HA-E 3888 bp ds-DNA circular SYN 23-FEB-2023 DEFINITION . ACCESSION VERSION . KEYWORDS KF718967- pGGD000. SOURCE synthetic DNA construct (unknown) ORGANISM synthetic DNA construct. REFERENCE 1 (bases 1 to 3749) AUTHORS Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU and Forner J. TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis JOURNAL PLoS ONE 8 (12), E83043 (2013) PUBMED 24376629 REFERENCE 2 (bases 1 to 3749) AUTHORS Forner J. TITLE Direct Submission JOURNAL Submitted (28-SEP-2013) Centre for Organismal Studies, Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230, Heidelberg D-69221, Germany REFERENCE 3 (bases 1 to 3749) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Jan 11, 2018 from SnapGene Viewer 3.2.1 http://www.snapgene.com COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##. FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="D" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; D" /label="D" Linker 20..214 /cds_type="ORF" /modified_by="mamee" /label="13xGly4Ser" CDS 218..1180 /label="TurboID" tag 1184..1210 /cds_type="ORF" /modified_by="mamee" /label="1xHA" GG complement(1214..1224) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="E" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; E" /label="E" primer complement(1244..1264) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 1744..1848 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 1849..2709 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 2880..3468 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 3832..3853 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcatca gcagtgggag gtggcggagg ttctggcggt ggaggatccg gaggtggcgg 61 gtccggtggt ggcggatcag gtggaggagg ttcgggaggc ggaggtagtg gaggcggtgg 121 gagcggcgga ggtgggagcg gtggcggagg gagtggaggt ggaggctcgg gcggaggtgg 181 ctccggcgga ggtgggtctg gaggcggtgg ctcggacaaa gacaataccg tgcccctcaa 241 actaattgca ttgcttgcca atggtgagtt ccattctggg gaacaacttg gggagactct 301 gggcatgtcc cgagcggcca ttaataagca catacagact ttgagggact ggggtgtcga 361 tgtgtttaca gtaccgggta agggatattc tctgccggag ccgatacctt tactcaatgc 421 aaagcagatt ttaggacagc tggatggcgg gagcgtggct gtcctcccgg tggtcgatag 481 tacaaatcaa tatcttttgg acaggatcgg tgagctgaaa tctggagatg cttgcatagc 541 ggaataccaa caagctggta ggggatctcg aggccgaaag tggttttcac cctttggggc 601 caacttatac ttatccatgt tctggcgtct gaaacgtggc ccggcagcca tagggctagg 661 gcccgtaatt gggatcgtta tggcggaagc gttacgaaag ttgggagccg ataaagtcag 721 agtaaagtgg ccaaatgatc tttatttaca agatagaaag ctggcgggca ttcttgttga 781 gcttgccgga ataacaggcg atgccgcaca gatcgttata ggggccggga ttaatgtggc 841 tatgaggagg gtcgaagaaa gtgttgtgaa tcaaggatgg attactctcc aagaggcggg 901 tataaatctt gatcgaaata ctctagctgc aaccttgata cgagagctaa gagcagcact 961 agaattgttt gaacaagaag ggctggcccc atatctccct cgatgggaga agttggacaa 1021 ttttataaac agaccggtga agttaatcat tggagacaaa gaaattttcg gtataagccg 1081 tgggatcgat aaacagggtg ctcttctcct tgagcaggac ggtgtcatca agccttggat 1141 gggaggcgaa atctctctcc gaagcgccga gaagaagctt gcctacccat acgatgttcc 1201 agattacgct tagctgctga gaccgaattc tcgccctata gtgagtcgta ttacctggcc 1261 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 1321 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 1381 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat 1441 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca 1501 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg 1561 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg 1621 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta 1681 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat 1741 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 1801 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 1861 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 1921 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 1981 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 2041 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 2101 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 2161 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 2221 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 2281 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 2341 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 2401 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 2461 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 2521 gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg aggtatcatt 2581 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 2641 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 2701 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 2761 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 2821 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 2881 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 2941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 3001 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 3061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 3121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 3181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 3241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 3301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 3361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 3421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 3481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 3541 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 3601 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 3661 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 3721 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 3781 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 3841 taacaatttc acacgagcta tttaggtgac actatagaag tgaagctt //