LOCUS pEN-L4-HA-TurboID-linker-R1 4080 bp DNA circular UNA 20-MAR-2023 DEFINITION . ACCESSION urn.local...ks-citr5zw KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers attL4 1..97 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attL4" /Transferred_Similarity="98.97%" /Primary_Match="98.97%; Adjusted-Interval-0:0/0; attL4" UTR 98..129 /modified_by="mamee" /label="omega leader" tag 130..213 /cds_type="ORF" /modified_by="mamee" /label="3xHA" CDS 217..1179 /cds_type="ORF" /label="TurboID" Linker 1183..1377 /cds_type="ORF" /modified_by="mamee" /label="13x Gly4Ser-linker" attR1 1378..1502 /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR1" /Transferred_Similarity="97.60%" /Primary_Match="97.60%; Adjusted-Interval-0:0/0; attR1" primer complement(1557..1577) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="95.24%" /Primary_Match="95.24%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" primer complement(1577..1598) /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" rep_origin complement(1962..2550) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" misc_feature complement(2728..3537) /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" promoter complement(3538..3588) /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" primer 4029..4044 /invitrogen=1980000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-20) forward primer (pDONRP4-P3 - M13 (-20) forward primer)" /label="M13 (-20) forward primer" ORIGIN 1 taaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa 61 tgctttttta taatgccaac tttgtataga aaagttgaac aacaaacaac aaacaacatt 121 acaattacaa tgtacccata cgatgttcca gattacgctt acccatacga tgttccagat 181 tacgcttacc catacgatgt tccagattac gctgccaaag acaataccgt gcccctcaaa 241 ctaattgcat tgcttgccaa tggtgagttc cattctgggg aacaacttgg ggagactctg 301 ggcatgtccc gagcggccat taataagcac atacagactt tgagggactg gggtgtcgat 361 gtgtttacag taccgggtaa gggatattct ctgccggagc cgataccttt actcaatgca 421 aagcagattt taggacagct ggatggcggg agcgtggctg tcctcccggt ggtcgatagt 481 acaaatcaat atcttttgga caggatcggt gagctgaaat ctggagatgc ttgcatagcg 541 gaataccaac aagctggtag gggatctcga ggccgaaagt ggttttcacc ctttggggcc 601 aacttatact tatccatgtt ctggcgtctg aaacgtggcc cggcagccat agggctaggg 661 cccgtaattg ggatcgttat ggcggaagcg ttacgaaagt tgggagccga taaagtcaga 721 gtaaagtggc caaatgatct ttatttacaa gatagaaagc tggcgggcat tcttgttgag 781 cttgccggaa taacaggcga tgccgcacag atcgttatag gggccgggat taatgtggct 841 atgaggaggg tcgaagaaag tgttgtgaat caaggatgga ttactctcca agaggcgggt 901 ataaatcttg atcgaaatac tctagctgca accttgatac gagagctaag agcagcacta 961 gaattgtttg aacaagaagg gctggcccca tatctccctc gatgggagaa gttggacaat 1021 tttataaaca gaccggtgaa gttaatcatt ggagacaaag aaattttcgg tataagccgt 1081 gggatcgata aacagggtgc tcttctcctt gagcaggacg gtgtcatcaa gccttggatg 1141 ggaggcgaaa tctctctccg aagcgccgag aagaagcttg acggtggcgg aggttctggc 1201 ggtggaggat ccggaggtgg cgggtccggt ggtggcggat caggtggagg aggttcggga 1261 ggcggaggta gtggaggcgg tgggagcggc ggaggtggga gcggtggcgg agggagtgga 1321 ggtggaggct cgggcggagg tggctccggc ggaggtgggt ctggaggcgg tggctcggca 1381 agtttgtaca aaaaagttga acgagaaacg taaaatgata taaatatcaa tatattaaat 1441 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat gcagtcacta 1501 tgatcggatc ccgggcccgt cgactgcaga ggcctgcatg caagcttggt gtaatcatgg 1561 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 1621 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 1681 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 1741 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 1801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 1861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 1921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 1981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 2041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 2101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 2161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 2221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 2281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 2341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 2401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 2461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 2521 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 2581 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 2641 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaagccc aatctgaata 2701 atgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg 2761 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga 2821 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat 2881 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc 2941 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat 3001 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc 3061 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt 3121 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc 3181 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg tttttccggg 3241 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg 3301 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc 3361 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaagcg 3421 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc 3481 agcatccatg ttggaattta atcgcggcct cgacgtttcc cgttgaatat ggctcataac 3541 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt 3601 atcttgtgca atgtaacatc agagattttg agacacgggc cagagctgca tcgcgcgttt 3661 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 3721 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 3781 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 3841 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 3901 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 3961 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 4021 acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa tacatctaga //