LOCUS pK7m34GW-GFP-BioID2-FLAG 11178 bp ds-DNA circular SYN 20-MAR-2023 DEFINITION . ACCESSION VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid. REFERENCE 1 (bases 1 to 11178) AUTHORS VIB Network TITLE Direct Submission JOURNAL Exported Sep 10, 2020 from SnapGene 4.2.0 http://www.snapgene.com FEATURES Location/Qualifiers Promoter 8..289 /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="96.81%" /Primary_Match="96.81%; Adjusted-Interval-0:0/0; P1 (SpR)" /label="P1" misc_feature 290..1081 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" rep_origin 1325..1913 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.83%" /Primary_Match="99.83%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" regulatory complement(1949..2209) /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:30/150; bom" /label="pBR322 bom" rep_origin 2583..2777 /note="origin of replication from Pseudomonas pVS1 plasmid" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 oriV" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV" /label="pVS1 oriV" regulatory complement(2843..3915) /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" regulatory complement(4344..4973) /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" T-DNA_border 6159..6358 /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="RB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; RB" /label="RB" primer 6385..6405 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="95.24%" /Primary_Match="95.24%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 6512..7548 /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="p35S" /Transferred_Similarity="98.75%" /Primary_Match="98.75%; Adjusted-Interval-0:0/0; p35S" /label="p35S" CDS 7603..8319 /codon_start=1 /note="vntifkey=4" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK" /label="GFP" CDS 8347..8361 /codon_start=1 /translation="GGGGS" /label="GGGGS" CDS 8362..9057 /codon_start=1 /product="promiscuous R40G mutant of a biotin protein ligase from Aquifex aeolicus (Kim et al., 2016)" /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKW LSQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKK VSGVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRI SENLKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEG IKEILSGEFSLRRS" /label="BioID2" tag 9058..9081 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /translation="DYKDDDDK" /modified_by="mamee" /label="FLAG" Stop 9082..9084 /created_by="mamee" /label="STOP" primer complement(9114..9129) /invitrogen=1980000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-20) forward primer (pDONRP4-P3 - M13 (-20) forward primer)" /label="M13 (-20) forward primer" terminator 9173..9412 /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="35ST" /Transferred_Similarity="95.42%" /Primary_Match="95.42%; Adjusted-Interval-0:0/0; t35S (35ST)" /label="t35S" Plant_Selection 9437..10825 /created_by="mamee" /label="Kana" promoter 9437..9724 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pNOS" /Transferred_Similarity="94.79%" /Primary_Match="94.79%; Adjusted-Interval-0:0/0; pNOS" /label="pNOS" CDS 9737..10531 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="NptII" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; NptII" /label="NptII" terminator 10558..10825 /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="NOST" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (NOST)" /label="tNOS" T-DNA_border 10846..11178 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="LB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB" /label="LB" ORIGIN 1 attcgggcac gaacccagtg gacataagcc tcgttcggtt cgtaagctgt aatgcaagta 61 gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc 121 gcagtggcgg ttttcatggc ttcttgttat gacatgtttt tttggggtac agtctatgcc 181 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag 241 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc 301 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct 361 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc 421 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg 481 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg 541 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa 601 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc 661 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc 721 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga 781 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg 841 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc 901 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat 961 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga 1021 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata 1081 atgtctagct agaaattcgt tcaagccgac gccgcttcgc cggcgttaac tcaagcgatt 1141 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta 1201 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa 1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 1441 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 1981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 2041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 2101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc 2161 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg 2221 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc 2281 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 2341 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc 2401 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc 2461 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg 2521 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca 2581 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg 2641 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt 2701 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa 2761 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt 2821 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc 2881 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg 2941 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg 3001 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg 3061 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg 3121 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag 3181 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc 3241 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac 3301 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct 3361 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca 3421 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg 3481 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg 3541 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac 3601 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt 3661 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg 3721 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt 3781 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg 3841 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg 3901 gtttgcgacc gtcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg 3961 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc 4021 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt 4081 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag 4141 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc 4201 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt 4261 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct 4321 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc 4381 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg 4441 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac 4501 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg 4561 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc 4621 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg 4681 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg 4741 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca 4801 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg 4861 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg 4921 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc 4981 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc 5041 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca 5101 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc 5161 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct 5221 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg 5281 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc 5341 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc 5401 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc 5461 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg 5521 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat 5581 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg 5641 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat 5701 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac 5761 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg 5821 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc 5881 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc 5941 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat 6001 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt 6061 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc 6121 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg 6181 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat 6241 ccgttattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac 6301 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctaagcttg 6361 ggcccgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcta 6421 tcaactttgt atagaaaagt tgtcatttag gtgacactat agaatactca agctatgcat 6481 ccaacgcgtt gggagctctc ccatatggtc gactagagcc aagctgatct cctttgcccc 6541 ggagatcacc atggacgact ttctctatct ctacgatcta ggaagaaagt tcgacggaga 6601 aggtgacgat accatgttca ccaccgataa tgagaagatt agcctcttca atttcagaaa 6661 gaatgctgac ccacagatgg ttagagaggc ctacgcggca ggtctcatca agacgatcta 6721 cccgagtaat aatctccagg agatcaaata ccttcccaag aaggttaaag atgcagtcaa 6781 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag 6841 tactattcca gtatggacga ttcaaggctt gcttcataaa ccaaggcaag taatagagat 6901 tggagtctct aagaaagtag ttcctactga atcaaaggcc atggagtcaa aaattcagat 6961 cgaggatcta acagaactcg ccgtgaagac tggcgaacag ttcatacaga gtcttttacg 7021 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc tcgtctactc 7081 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag 7141 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag 7201 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat 7261 cgttcaagat gcccctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat 7321 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc 7381 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata 7441 aggaagttca tttcatttgg agaggactgc aggacgatcc gtatttttac aacaattacc 7501 acaacaaaac aaacaacaaa caacattaca atttactatt ctagtcgacc tgcaggcggc 7561 cgcactagtg atatcacaag tttgtacaaa aaagcaggct taatggtgag caagggcgag 7621 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 7681 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 7741 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc 7801 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag 7861 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 7921 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 7981 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 8041 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc 8101 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 8161 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc 8221 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 8281 gccgccggga tcactctcgg catggacgag ctgtacaagt acccagcttt cttgtacaaa 8341 gtggccggag gcggtggatc gttcaagaac ctgatctggc tgaaggaggt ggacagcacc 8401 caggagagac tgaaggagtg gaacgtgagc tacggcaccg ccctggtggc cgacagacag 8461 accaagggca gaggcggcct gggcagaaag tggctgagcc aggagggcgg cctgtacttc 8521 agcttcctgc tgaaccccaa ggagttcgag aacctgctgc agctgcccct ggtgctgggc 8581 ctgagcgtga gcgaggccct ggaggagatc accgagatcc ccttcagcct gaagtggccc 8641 aacgacgtgt acttccagga gaagaaggtg agcggcgtgc tgtgcgagct gagcaaggac 8701 aagctgatcg tgggcatcgg catcaacgtg aaccagagag agatccccga ggagatcaag 8761 gacagagcca ccaccctgta cgagatcacc ggcaaggact gggacagaaa ggaggtgctg 8821 ctgaaggtgc tgaagagaat cagcgagaac ctgaagaagt tcaaggagaa gagcttcaag 8881 gagttcaagg gcaagatcga gagcaagatg ctgtacctgg gcgaggaggt gaagctgctg 8941 ggcgagggca agatcaccgg caagctggtg ggcctgagcg agaagggcgg cgccctgatc 9001 ctgaccgagg agggcatcaa ggagatcctg agcggcgagt tcagcctgag aagaagcgac 9061 tacaaagacg atgacgataa atgacaactt tattatacat agttgataat tcactggccg 9121 tcgttttaca acgtcgtgac tgggaaaacg atctaactga ctagccgcgg ccatggcggc 9181 cgggagcggc catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct 9241 ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta 9301 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa 9361 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac ctgcaggtcg 9421 accatatggg agagctcgaa ttatcataca tgagaattaa gggagtcacg ttatgacccc 9481 cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag 9541 gagccactca gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat 9601 tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa 9661 atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc 9721 tcaactcgat cgaggcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 9781 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 9841 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 9901 tgccctgaat gaactccaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt 9961 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 10021 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 10081 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 10141 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 10201 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 10261 ggcgcggatg cccgacggcg aggatctcgt cgtgacccac ggcgatgcct gcttgccgaa 10321 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 10381 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 10441 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 10501 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgg actctagcta 10561 gagtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc 10621 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa 10681 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata 10741 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc 10801 ggtgtcatct atgttactag atcgaccggc atgcaagctg ataattcaat tcggcgttaa 10861 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta 10921 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa 10981 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg 11041 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc 11101 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag 11161 cgttgctgcc tgtgatca //