LOCUS pGGIB-U6-A-ccdB-G-U7 3900 bp DNA circular 06-FEB-2023 DEFINITION . ACCESSION p01678 VERSION urn.local...3dt-ak3eymm KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_difference 1..18 /created_by="laurenspauwels" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="I-SceI site" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; I-SceI site" /label="I-SceI site" misc_feature 19..58 /created_by="lapau" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="U6" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; U6" /label="U6" /note="Geneious type: Golden Gibson Cloning site" GG complement(67..77) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="A" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; A" /label="A" CDS complement(84..389) /Transferred_From="ccdB" /Transferred_Similarity="99.67%" /label="ccdB" UTR complement(390..708) /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.37%" /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" misc_feature complement(731..1390) /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="99.85%" /Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" promoter complement(1444..1474) /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="lacUV5" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (CmR)" /label="lacUV5" GG 1491..1501 /%_Identity=100 /Motif="GGTCTCAGTAT" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="G" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; G" /label="G" misc_feature 1508..1547 /created_by="lapau" /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="U7" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; U7" /label="U7" /note="Geneious type: Golden Gibson Cloning site" misc_difference complement(1548..1565) /created_by="laurenspauwels" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="I-SceI site" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; I-SceI site" /label="I-SceI site" primer complement(1596..1612) /invitrogen=2010000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 reverse primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 reverse primer (pDONRP4-P3 - M13 reverse primer)" /label="M13 reverse primer" promoter 1674..1724 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 1725..2534 /modified_by="wacae" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 2681..3269 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 3869..3884 /invitrogen=1980000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-20) forward primer (pDONRP4-P3 - M13 (-20) forward primer)" /label="M13 (-20) forward primer" ORIGIN 1 attaccctgt tatccctact cgttcgctgc cacctaagaa tactctacgg tcacatacct 61 cgagatacct tgagaccgtc gacttatatt ccccagaaca tcaggttaat ggcgtttttg 121 atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga gactggcaca 181 ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa 241 agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt 301 cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata acggctctct 361 cttttatagg tgtaaacctt aaactgcatt tcaccagtcc ctgttctcgt cagcaaaaga 421 gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca 481 gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca gaccggagat 541 attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata 601 cgctgcttca tagcacacct ctttttgaca tacttcgggt atacatatca gtatatattc 661 ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt agtaagccgg 721 atccacgcgt ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca 781 ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca 841 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt 901 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga 961 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca 1021 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg 1081 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata 1141 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg 1201 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa 1261 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg 1321 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt 1381 ttttctccat tttagcttcc ttagctcctg aaaatctcgc cggatcctaa ctcaaaatcc 1441 acacattata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat ggtctcagta 1501 ttctagacaa gacgctggct ctgacatttc cgctactgaa ctactcgtag ggataacagg 1561 gtaatgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct 1621 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa 1681 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac 1741 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat 1801 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg 1861 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg 1921 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta 1981 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc 2041 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc 2101 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc 2161 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg 2221 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat 2281 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg 2341 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg 2401 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt 2461 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg 2521 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga 2581 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc 2641 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 2701 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 2761 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 2821 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 2881 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 2941 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 3001 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 3061 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 3121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 3181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 3241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 3301 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 3361 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 3421 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 3481 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 3541 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa 3601 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg 3661 tcctgcccgc caccccttcg ggccgttgct tcacaacgtt caaatccgct cccggcggat 3721 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtcat 3781 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta 3841 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc //