LOCUS pGG-C-mRuby3-D 3399 bp DNA circular SYN 23-FEB-2023 DEFINITION . ACCESSION p00238 KEYWORDS . SOURCE Cloning vector pGGC000 (unknown) ORGANISM Cloning vector pGGC000 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3749) AUTHORS Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U. and Forner,J. TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis JOURNAL PLoS ONE 8 (12), E83043 (2013) PUBMED 24376629 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 3749) AUTHORS Forner,J. TITLE Direct Submission JOURNAL Submitted (28-SEP-2013) Centre for Organismal Studies, Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230, Heidelberg D-69221, Germany COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="C" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; C" /label="C" CDS 14..724 /note="added by Rabuo" /modified_by="wacae" /Transferred_From="mRuby3" /Transferred_Similarity="100.00%" /label="mRuby3" Source join(<725..1159,1160..>7) /organism="Cloning vector pGGC000" /mol_type="other DNA" /db_xref="taxon:1439662" GG complement(725..735) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="D" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; D" /label="D" primer complement(755..775) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 1255..1359 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 1360..2220 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 2391..2979 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 3343..3364 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcaggc tccatggtgt ctaagggcga agagctgatc aaggaaaata tgcgtatgaa 61 ggtggtcatg gaaggttcgg tcaacggcca ccaattcaaa tgcacaggtg aaggagaagg 121 cagaccgtac gagggaactc aaaccatgag gatcaaagtc atcgagggag gacccctgcc 181 atttgccttt gacattcttg ccacgtcgtt catgtatggc agccgtactt ttatcaagta 241 cccggccgac atccctgatt tctttaaaca gtcctttcct gagggtttta cttgggaaag 301 agttacgaga tacgaagatg gtggagtcgt caccgtcacg caggacacca gccttgagga 361 tggcgagctc gtctacaacg tcaaggtcag aggggtaaac tttccctcca atggtcccgt 421 gatgcagaag aagaccaagg gttgggagcc taatacagag atgatgtatc cagcagatgg 481 tggtctgaga ggatacactg acatcgcact gaaagttgat ggtggtggcc atctgcactg 541 caacttcgtg acaacttaca ggtcaaaaaa gaccgtcggg aacatcaaga tgcccggtgt 601 ccatgccgtt gatcaccgcc tggaaaggat cgaggagagt gacaatgaaa cctacgtagt 661 gcaacgcgaa gtggcagttg ccaaatacag caaccttggt ggtggcatgg acgagctgta 721 caagtcagtg agaccgaatt ctcgccctat agtgagtcgt attacctggc cgtcgtttta 781 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 841 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 901 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt 961 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc 1021 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 1081 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 1141 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat 1201 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga 1261 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 1321 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 1381 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 1441 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 1501 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 1561 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 1621 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 1681 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 1741 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 1801 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 1861 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 1921 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 1981 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 2041 tttattgctg ataaatctgg agccggtgag cgtggctctc gaggtatcat tgcagcactg 2101 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 2161 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 2221 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 2281 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 2341 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 2401 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 2461 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 2521 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 2581 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 2641 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 2701 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 2761 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 2821 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 2881 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 2941 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 3001 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 3061 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 3121 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 3181 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 3241 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag 3301 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt 3361 cacacgagct atttaggtga cactatagaa gtgaagctt //