LOCUS pEN-L4-Mito-GSrhino-R1 3329 bp DNA circular SYN 15-MAR-2023 DEFINITION . ACCESSION pDONRP4-P1R KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/ ORIGDB|GenBank VNTDBDATE|-12271295| LSOWNER| VNTNAME|pEN-L4R1-Mito-GSrhino| VNTAUTHORNAME|Demo User| VNTOAUTHORNAME|UNKNOWN| Vector_NTI_Display_Data_(Do_Not_Edit!) 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FEATURES Location/Qualifiers attL4 1..97 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attL4" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL4" CDS 126..221 /modified_by="mamee" /label="Mito" CDS 222..782 /label="NGSrhino" Cleavage_Site 600..647 /created_by="mamee" /label="2x 3C Rhino" Tag 654..767 /created_by="mamee" /label="SBP" attR1 784..940 /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR1" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1" primer complement(1003..1019) /invitrogen=2010000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 reverse primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 reverse primer (pDONRP4-P3 - M13 reverse primer)" /label="M13 reverse primer" promoter 1081..1131 /created_by="chgai" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 1132..1941 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" rep_origin 2088..2676 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.49%" /Primary_Match="99.49%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 3255..3271 /invitrogen=2000000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-40) forward primer (pDONRP4-P3 - M13 (-40) forward primer)" /label="M13 (-40) forward primer" primer 3275..3290 /invitrogen=1980000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-20) forward primer (pDONRP4-P3 - M13 (-20) forward primer)" /label="M13 (-20) forward primer" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa 61 tgctttttta taatgccaac tttgtataga aaagttgaac aacaaacaac aaacaacatt 121 caaccatgtt gtcactacgt caatctataa gatttttcaa gccagccaca agaactttgt 181 gtagctctag atatctgctt cagcaaaaac ccatggaatt catgggcacc cccgcagtca 241 ccacctacaa gctggtcatt aacggcaaga ctctgaaggg cgagaccacc accaaggccg 301 tggacgcaga aaccgcggaa aaggcgttca agcagtacgc gaacgacaac ggggtggacg 361 gagtctggac ctacgatgac gctacaaaaa cgttcaccgt gaccgaggtt aacacccccg 421 ctgtgactac gtacaagctg gtgatcaatg ggaagaccct gaagggcgag accaccacga 481 aagctgtaga cgccgagaca gccgagaagg ccttcaagca gtacgccaat gacaacggcg 541 tggatggcgt gtggacctat gacgacgcca cgaagacatt cactgtgact gagatcgatc 601 ttgaagttct ttttcaagga cctcttgaag ttctttttca aggacctggc agtatggacg 661 agaagaccac cggctggaga ggcggccacg tggtcgaggg actggccggt gagctggagc 721 agctacgggc ccgccttgag caccaccccc aggggcagcg cgagccgggt ggaggcggtt 781 cagcaagttt gtacaaaaaa gttgaacgag aaacgtaaaa tgatataaat atcaatatat 841 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatgcagt 901 cactatgaat caactactta gatggtatta gtgacctgta gaattcgagc tctagagctg 961 cagggcggcc gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg 1021 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca 1081 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat 1141 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg 1201 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg 1261 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt 1321 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag 1381 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca 1441 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca 1501 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc 1561 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat 1621 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt 1681 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt 1741 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga 1801 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa 1861 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg 1921 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt 1981 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg 2041 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 2101 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 2161 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 2221 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 2281 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 2341 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 2401 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 2461 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac 2521 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 2581 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 2641 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 2701 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 2761 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 2821 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 2881 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 2941 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa 3001 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg 3061 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg 3121 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc 3181 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta 3241 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc 3301 gggcccgcgt taacgctacc atggagctc //