LOCUS pEN-L4-Chl-GSyellow-R1 3860 bp DNA circular SYN 15-MAR-2023
DEFINITION .
ACCESSION pDONRP4-P1R
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDBDATE|-12271289|
LSOWNER|
VNTNAME|pEN-L4R1-Chl-GSyellow|
VNTAUTHORNAME|Demo User|
VNTOAUTHORNAME|UNKNOWN|
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FEATURES Location/Qualifiers
attL4 1..97
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attL4"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL4"
CDS 126..320
/modified_by="mamee"
/label="Chloro"
CDS 321..1298
/label="NGSyellow"
CDS 321..1037
/created_by="mamee"
/label="Citrine YFP"
Cleavage_Site 1038..1085
/created_by="mamee"
/label="2x 3C Rhino"
Cleavage_Site 1086..1184
/created_by="mamee"
/label="2x TEV"
Tag 1185..1298
/created_by="mamee"
/label="SBP"
attR1 1315..1471
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR1"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1"
primer complement(1534..1550)
/invitrogen=2010000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 reverse primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
reverse primer (pDONRP4-P3 - M13 reverse primer)"
/label="M13 reverse primer"
promoter 1612..1662
/created_by="chgai"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2023.0.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 1663..2472
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
rep_origin 2619..3207
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.49%"
/Primary_Match="99.49%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 3786..3802
/invitrogen=2000000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-40) forward primer (pDONRP4-P3 - M13 (-40) forward
primer)"
/label="M13 (-40) forward primer"
primer 3806..3821
/invitrogen=1980000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-20) forward primer (pDONRP4-P3 - M13 (-20) forward
primer)"
/label="M13 (-20) forward primer"
ORIGIN
1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
61 tgctttttta taatgccaac tttgtataga aaagttgaac aacaaacaac aaacaacatt
121 caaccatggc gacggctttc gctcccacta agctcactgc cacggttcct ctgcatggat
181 cccatgagaa tcgtctcttg ctcccgatcc gattggctcc tccttcttct ttcctcggat
241 ccacccgttc cctctccctt cgcagactca atcactccaa cgccacccgt cgatctcccg
301 tcgtctctgt ccaggaagtt atggtgagca agggcgagga gctgttcacc ggggtggtgc
361 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
421 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
481 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc
541 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
601 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
661 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
721 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
781 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
841 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
901 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
961 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
1021 tggacgagct gtacaagctt gaagttcttt ttcaaggacc tcttgaagtt ctttttcaag
1081 gacctgagaa tttgtatttt cagggtgcta ttcccgggac tactgagaat ttgtattttc
1141 agggtgagct caaaaccgcg gctcttgggc aacacgatga agccatggac gagaagacca
1201 ccggctggag aggcggccac gtggtcgagg gacttgccgg tgaacttgag cagctaagag
1261 cacgtcttga gcatcaccca caggggcaac gtgagcctgg tggaggcggt tcagcaagtt
1321 tgtacaaaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
1381 ttttgcataa aaaacagact acataatact gtaaaacaca acatatgcag tcactatgaa
1441 tcaactactt agatggtatt agtgacctgt agaattcgag ctctagagct gcagggcggc
1501 cgcgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
1561 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
1621 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
1681 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
1741 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
1801 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
1861 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
1921 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
1981 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
2041 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
2101 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
2161 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
2221 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
2281 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
2341 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
2401 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
2461 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
2521 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
2581 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
2641 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
2701 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
2761 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
2821 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
2881 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
2941 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
3001 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
3061 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
3121 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
3181 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
3241 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
3301 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
3361 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
3421 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
3481 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
3541 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
3601 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
3661 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
3721 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
3781 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggcccgcg
3841 ttaacgctac catggagctc
//