Vector ID: 3_77
Application
Transcriptional Reporter
Fusion Protein
Species
Plants
Backbone
Binary
Bacterial selection
Spectinomycin
Vector type
Third Party
GW Destination
Plant selectable marker
Hygromycin
Fluorescent Seed
Bacterial strain
unknown
Created
Remarks
FAST-R: Some transgenic lines with FAST-R gave red fluorescence in true leaves, while none of transgenic lines with FAST-G gave fluorescence in true leaves.
The linker sequence between a gene of your interest and GFP is composed of 53 bp (see below), which is not a multiple number of 3. To avoid a frame shift, please add one base (G or C) to 3' side of the gene of your interest.
The linker sequence: [a gene of your interest] Gaagggtgggcgcgccgacccagctttcttgtacaaagtggtgatatcccgcgg [GFP]
Publications
Overhang
R1-R2
